EY702597 | Citrus Genome Database

Overview


Name	EY702597
Unique Name	EY702597
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	871

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.422e-27
Identity = 61/76 (80.26%), Postives = 66/76 (86.84%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            MQIFVKTLTGKTI LEV  SD ++NVKAKIQDKEGIPPDQQRL FA KQLE+GRTL+DYN+ KE  LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 92.8189 bits (229), Expect = 6.955e-25
Identity = 44/46 (95.65%), Postives = 46/46 (100.00%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            QDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   31 QDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 42.743 bits (99), Expect = 6.955e-25
Identity = 21/23 (91.30%), Postives = 21/23 (91.30%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            MQIFVKTLTGKTI LEVE SDTI
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTI 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 7.057e-27
Identity = 60/76 (78.95%), Postives = 65/76 (85.53%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            MQIFVKTLTG TI LEV  SD ++NVKAKIQDKEGIPPDQQRL FA KQLE+GRTL+DYN+ KE  LHLVL LRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 90.1225 bits (222), Expect = 2.141e-23
Identity = 43/46 (93.48%), Postives = 45/46 (97.83%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            QDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   31 QDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 40.4318 bits (93), Expect = 2.141e-23
Identity = 20/23 (86.96%), Postives = 20/23 (86.96%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            MQIFVKTLTG TI LEVE SDTI
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTI 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.377e-22
Identity = 53/76 (69.74%), Postives = 59/76 (77.63%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            MQIFVKTLTGKTIT+E    D V  VK KI DKEG+P DQQRL +A KQLED +TLADYN+ KE  LH+VL LRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 83.9593 bits (206), Expect = 1.885e-21
Identity = 39/45 (86.67%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  149 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:   32 DKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 40.0466 bits (92), Expect = 1.885e-21
Identity = 18/23 (78.26%), Postives = 20/23 (86.96%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            MQIFVKTLTGKTIT+E E  DT+
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 104.375 bits (259), Expect = 8.927e-22
Identity = 50/76 (65.79%), Postives = 60/76 (78.95%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            MQIF+KTLTGKTIT E   ++ V ++K KI DKEG+P DQQRL FA KQLED +T+ADYN+ KE  LH+VL LRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 84.7297 bits (208), Expect = 5.405e-21
Identity = 39/45 (86.67%), Postives = 43/45 (95.56%), Query Frame = 2

Query:  149 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:   32 DKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 37.7354 bits (86), Expect = 5.405e-21
Identity = 16/23 (69.57%), Postives = 20/23 (86.96%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            MQIF+KTLTGKTIT E E ++T+
Sbjct:    1 MQIFIKTLTGKTITAETEPAETV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 5.786e-21
Identity = 50/63 (79.37%), Postives = 54/63 (85.71%), Query Frame = 2

Query:  317 TITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLR 505
            TITLEV  SD ++NVKAKIQDKEGIPPDQQRL FA KQLEDGRTL+DYN+ KE  LHLVL LR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 89.7373 bits (221), Expect = 2.294e-18
Identity = 43/44 (97.73%), Postives = 44/44 (100.00%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 277
            QDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:   20 QDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 3 Score: 23.8682 bits (50), Expect = 2.294e-18
Identity = 11/12 (91.67%), Postives = 11/12 (91.67%), Query Frame = 1

Query:   88 TITLEVESSDTI 123
            TITLEVE SDTI
Sbjct:    1 TITLEVEPSDTI 12

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 3.881e-17
Identity = 43/80 (53.75%), Postives = 56/80 (70.00%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGGMQIL 523
            M I VKTLTGK I +++  +D VD +K ++++KEGIPP QQRL F+ KQ+ D +T ADY +     LHLVL LRGG  IL
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSIL 80

HSP 2 Score: 68.9366 bits (167), Expect = 2.379e-13
Identity = 32/49 (65.31%), Postives = 38/49 (77.55%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 292
            ++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:   31 EEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 3 Score: 27.7202 bits (60), Expect = 2.379e-13
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I +++E +D +
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 1.129e-16
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGGMQIL 523
            I VKTLTGK I +++  +D +D +K ++++KEGIPP QQRL +A KQL D +T  DYN+     LHLVL LRGG  +L
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGFGLL 80

HSP 2 Score: 67.3958 bits (163), Expect = 2.379e-13
Identity = 32/46 (69.57%), Postives = 37/46 (80.43%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   31 EEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 3 Score: 29.261 bits (64), Expect = 2.379e-13
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:   61 IFVKTLTGKTITLEVESSDTI 123
            I VKTLTGK I +++E +DTI
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTI 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.475e-16
Identity = 43/76 (56.58%), Postives = 54/76 (71.05%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I L++  +D V+ +K K+++KEGIPP QQRL FA KQ+ D +T ADY +     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 66.6254 bits (161), Expect = 6.782e-13
Identity = 31/46 (67.39%), Postives = 36/46 (78.26%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:   31 EEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 3 Score: 28.4906 bits (62), Expect = 6.782e-13
Identity = 13/23 (56.52%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I L++E +D +
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.516e-16
Identity = 41/74 (55.41%), Postives = 53/74 (71.62%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            I VKTLTGK I +++  +D +D VK ++++KEGIPP QQRL +A KQL D +T  DYN+     LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 2.379e-13
Identity = 32/46 (69.57%), Postives = 37/46 (80.43%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   31 EEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 3 Score: 29.261 bits (64), Expect = 2.379e-13
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:   61 IFVKTLTGKTITLEVESSDTI 123
            I VKTLTGK I +++E +DTI
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTI 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.286e-16
Identity = 40/74 (54.05%), Postives = 53/74 (71.62%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            I VKTLTGK I +++  +D +D +K ++++KEGIPP QQRL +A KQL D +T  DYN+     LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 2.379e-13
Identity = 32/46 (69.57%), Postives = 37/46 (80.43%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   31 EEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 3 Score: 29.261 bits (64), Expect = 2.379e-13
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:   61 IFVKTLTGKTITLEVESSDTI 123
            I VKTLTGK I +++E +DTI
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTI 23

The following BLAST results are available for this feature:

BLAST of EY702597 vs. ExPASy Swiss-Prot

BLAST of EY702597 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 93


Match Name	E-value	Identity	Description
UBIQ_LEIMA	1.422e-27	80.26	Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1	[more]
UBIQ_LEITA	7.057e-27	78.95	Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3...	[more]
UBIL_NPVOP	1.377e-22	69.74	Ubiquitin-like protein OS=Orgyia pseudotsugata mul...	[more]
UBIL_NPVAC	8.927e-22	65.79	Ubiquitin-like protein OS=Autographa californica n...	[more]
UBIQ_LUMTE	5.786e-21	79.37	Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ...	[more]
NEDD8_DROME	3.881e-17	53.75	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]
RUB1_ARATH	1.129e-16	52.56	NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN...	[more]
NEDD8_CAEEL	1.475e-16	56.58	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB1_DESAN	2.516e-16	55.41	NEDD8-like protein RUB1 OS=Deschampsia antarctica ...	[more]
RUB2_ORYSJ	3.286e-16	54.05	NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-701-009-H06-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY702597 ID=EY702597; Name=EY702597; organism=Citrus sinensis; type=EST; length=871bp
CCACGCGTCCGGAAGGAGTCATCCCTTCATCTCGTCCTCAGGCTCCGTGG
TGGTATGCAGATCTTCGTCAAAACCCTAACTGGGAAGACGATCACTCTGG
AGGTGGAGAGTTCGGATACGATTGATAGGCGGGGGGGGCAGGATCCAGGA
CAAAGAGGGGATCCCGCCGGACCAGCAAAGATTGATCTTCGCCGGTAAGC
AGTTGGAAGATGGCCGCACCTTGGCCGATTACAACATTCAGAAAGAGTCA
ACTCTCCACCTTGTTCTTCGTCTCAGGGGAGGCATGCAGATATTTGTTAA
GACCCTTACTGGGAAAACAATTACTTTGGAAGTTCATAGCTCAGACGCGG
TTGACAATGTCAAGGCAAAAATCCAAGATAAGGAAGGAATTCCACCGGAT
CAGCAGCGTTTGACCTTCGCTCGCAAACAGCTTGAGGATGGAAGGACTCT
TGCGGATTATAACCTCCACAAGGAGGTCGCCCTTCATCTGGTTCTGCCCC
TGAGGGGGGGTATGCAGATACTTGTCAAGACCCTTGACTGGGAAGACTAT
TACTCTGGAGGTGGAGAGCTCCGATACTATTGATAATGTTAAGGCAAAGA
TTCAGGACAACGAAGGGCATTCCCCCAGATCCAGAAAGGCTCATCTTTCC
TGGAAACCACCTTGAAGGAGGGCCGTCTTTACCTGATTATCACTTTCCCA
AGGGGCCCCCCCTCCCTCTTCGGGCTCCCCCTTCTTGGGGGTGAATTCCT
AAAAGGGTATCTCCCTTGGCCGCCGGGCCGCCTCCCCCCCGCCTGCACCC
AAAAATCTATTTTCCCTGCGGGCGCCCCCCGTCCTCTTCCAACTCTTTGC
CCCAAGCTTTACCGCGCAAAC