EY702597 | Citrus Genome Database

Overview


Name	EY702597
Unique Name	EY702597
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	871

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.286e-16
Identity = 40/74 (54.05%), Postives = 53/74 (71.62%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            I VKTLTGK I +++  +D +D +K ++++KEGIPP QQRL +A KQL D +T  DYN+     LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 67.3958 bits (163), Expect = 2.379e-13
Identity = 32/46 (69.57%), Postives = 37/46 (80.43%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   31 EEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 3 Score: 29.261 bits (64), Expect = 2.379e-13
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:   61 IFVKTLTGKTITLEVESSDTI 123
            I VKTLTGK I +++E +DTI
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTI 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 1.631e-15
Identity = 39/75 (52.00%), Postives = 53/75 (70.67%), Query Frame = 2

Query:  290 IFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGGM 514
            I VKTLTGK I +++  +D +D +K ++++KEGIPP QQRL +A KQL D +T  DY +     LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 65.0846 bits (157), Expect = 1.145e-12
Identity = 31/47 (65.96%), Postives = 37/47 (78.72%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 286
            ++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:   31 EEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 3 Score: 29.261 bits (64), Expect = 1.145e-12
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1

Query:   61 IFVKTLTGKTITLEVESSDTI 123
            I VKTLTGK I +++E +DTI
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTI 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.130e-15
Identity = 39/76 (51.32%), Postives = 54/76 (71.05%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I +++  +D V+ +K ++++KEGIPP QQRL ++ KQ+ D +T ADY +     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 64.6994 bits (156), Expect = 4.239e-12
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   31 EEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 27.7202 bits (60), Expect = 4.239e-12
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I +++E +D +
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.130e-15
Identity = 39/76 (51.32%), Postives = 54/76 (71.05%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I +++  +D V+ +K ++++KEGIPP QQRL ++ KQ+ D +T ADY +     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 64.6994 bits (156), Expect = 4.239e-12
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   31 EEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 27.7202 bits (60), Expect = 4.239e-12
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I +++E +D +
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 2.130e-15
Identity = 39/76 (51.32%), Postives = 54/76 (71.05%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I +++  +D V+ +K ++++KEGIPP QQRL ++ KQ+ D +T ADY +     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 64.6994 bits (156), Expect = 4.239e-12
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   31 EEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 27.7202 bits (60), Expect = 4.239e-12
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I +++E +D +
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.130e-15
Identity = 39/76 (51.32%), Postives = 54/76 (71.05%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I +++  +D V+ +K ++++KEGIPP QQRL ++ KQ+ D +T ADY +     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 64.6994 bits (156), Expect = 4.239e-12
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   31 EEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 27.7202 bits (60), Expect = 4.239e-12
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I +++E +D +
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.130e-15
Identity = 39/76 (51.32%), Postives = 54/76 (71.05%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I +++  +D V+ +K ++++KEGIPP QQRL ++ KQ+ D +T ADY +     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 64.6994 bits (156), Expect = 4.239e-12
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   31 EEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 27.7202 bits (60), Expect = 4.239e-12
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I +++E +D +
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.380e-14
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            + + V+TLTGK I +++  +D VD +K +++++EGIPP QQRL +  KQL D +T  DY +     LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 62.3882 bits (150), Expect = 1.568e-11
Identity = 29/46 (63.04%), Postives = 35/46 (76.09%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            +++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:   31 EEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 3 Score: 28.1054 bits (61), Expect = 1.568e-11
Identity = 10/23 (43.48%), Postives = 18/23 (78.26%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            + + V+TLTGK I +++E +DT+
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: UBL1_SCHPO (Ubiquitin-like protein 1 OS=Schizosaccharomyces pombe GN=ubl1 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.803e-14
Identity = 39/76 (51.32%), Postives = 51/76 (67.11%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M I VKTLTGK I L++  +D V  +K ++++KEGIPP QQRL +A KQ+ D +    Y+L     LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76

HSP 2 Score: 60.8474 bits (146), Expect = 7.529e-11
Identity = 27/46 (58.70%), Postives = 35/46 (76.09%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:   31 EEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76

HSP 3 Score: 27.335 bits (59), Expect = 7.529e-11
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M I VKTLTGK I L+++ +D +
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKV 23

[Close]

BLAST of EY702597 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.075e-14
Identity = 38/76 (50.00%), Postives = 51/76 (67.11%), Query Frame = 2

Query:  284 MQIFVKTLTGKTITLEVHSSDAVDNVKAKIQDKEGIPPDQQRLTFARKQLEDGRTLADYNLHKEVALHLVLPLRGG 511
            M+I VKTLT K I +E+  +D ++ +K +I++KEGIPP  QR+ +  KQL D  T   YNL +   LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 59.3066 bits (142), Expect = 4.463e-11
Identity = 26/46 (56.52%), Postives = 34/46 (73.91%), Query Frame = 2

Query:  146 QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 283
            ++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:   31 EEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 3 Score: 29.6462 bits (65), Expect = 4.463e-11
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 1

Query:   55 MQIFVKTLTGKTITLEVESSDTI 123
            M+I VKTLT K I +E+E +DTI
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTI 23

The following BLAST results are available for this feature:

BLAST of EY702597 vs. ExPASy Swiss-Prot

BLAST of EY702597 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 93


Match Name	E-value	Identity	Description
RUB1_ORYSJ	3.286e-16	54.05	NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap...	[more]
RUB2_ARATH	1.631e-15	52.00	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	2.130e-15	51.32	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	2.130e-15	51.32	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	2.130e-15	51.32	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	2.130e-15	51.32	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	2.130e-15	51.32	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	1.380e-14	47.37	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
UBL1_SCHPO	1.803e-14	51.32	Ubiquitin-like protein 1 OS=Schizosaccharomyces po...	[more]
RUB3_ARATH	3.075e-14	50.00	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-701-009-H06-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY702597 ID=EY702597; Name=EY702597; organism=Citrus sinensis; type=EST; length=871bp
CCACGCGTCCGGAAGGAGTCATCCCTTCATCTCGTCCTCAGGCTCCGTGG
TGGTATGCAGATCTTCGTCAAAACCCTAACTGGGAAGACGATCACTCTGG
AGGTGGAGAGTTCGGATACGATTGATAGGCGGGGGGGGCAGGATCCAGGA
CAAAGAGGGGATCCCGCCGGACCAGCAAAGATTGATCTTCGCCGGTAAGC
AGTTGGAAGATGGCCGCACCTTGGCCGATTACAACATTCAGAAAGAGTCA
ACTCTCCACCTTGTTCTTCGTCTCAGGGGAGGCATGCAGATATTTGTTAA
GACCCTTACTGGGAAAACAATTACTTTGGAAGTTCATAGCTCAGACGCGG
TTGACAATGTCAAGGCAAAAATCCAAGATAAGGAAGGAATTCCACCGGAT
CAGCAGCGTTTGACCTTCGCTCGCAAACAGCTTGAGGATGGAAGGACTCT
TGCGGATTATAACCTCCACAAGGAGGTCGCCCTTCATCTGGTTCTGCCCC
TGAGGGGGGGTATGCAGATACTTGTCAAGACCCTTGACTGGGAAGACTAT
TACTCTGGAGGTGGAGAGCTCCGATACTATTGATAATGTTAAGGCAAAGA
TTCAGGACAACGAAGGGCATTCCCCCAGATCCAGAAAGGCTCATCTTTCC
TGGAAACCACCTTGAAGGAGGGCCGTCTTTACCTGATTATCACTTTCCCA
AGGGGCCCCCCCTCCCTCTTCGGGCTCCCCCTTCTTGGGGGTGAATTCCT
AAAAGGGTATCTCCCTTGGCCGCCGGGCCGCCTCCCCCCCGCCTGCACCC
AAAAATCTATTTTCCCTGCGGGCGCCCCCCGTCCTCTTCCAACTCTTTGC
CCCAAGCTTTACCGCGCAAAC