CX077956

Overview
NameCX077956
Unique NameCX077956
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length860
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSA5_SCHPO (Probable proteasome subunit alpha type-5 OS=Schizosaccharomyces pombe GN=SPAC323.02c PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.890e-33
Identity = 77/211 (36.49%), Postives = 113/211 (53.55%), Query Frame = 3
Query:   60 YDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYW------------WLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKD 656
            YD  V TFSP+GR+FQ+EYA +A+    T IG+K KD +V+GVEK + S +M   S  ++  +  H G A++GL AD R I+  A+ +  N+   Y EP  ++   Q +      C L                RPFG  +++ G D  GPQLY  EPSG  +RY   AIG G + AK+E+ K    +MT  +  + + K++  V +E  D
Sbjct:    8 YDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSI------CDLALRFGEGEDGEERIMSRPFGVALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLILKVLRQVMEEKLD 212          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_PYRHO (Proteasome subunit alpha OS=Pyrococcus horikoshii GN=psmA PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 3.775e-33
Identity = 67/184 (36.41%), Postives = 108/184 (58.70%), Query Frame = 3
Query:   57 GYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGV 608
            GYD ++T FSPDGR+FQ+ YA +AV    T +G+KC +G+V+ VEK I S+++ P S  +I  +  H   A +G+ AD R +V RA+ EA  +   YGEP P+  + +++     + T Y  +RPFG  +++ G + + P+LY  +PSG  + +   AIG GR  A    E+    +M+  + +
Sbjct:    9 GYDRAITVFSPDGRLFQVNYAREAVKRGATAVGVKCNEGVVLAVEKRITSRLIEPESYEKIFQIDDHIAAASSGIIADARVLVNRARLEAQIHRLTYGEPAPLAVIVKKICDLKQMHTQYGGVRPFGAALLMAGVN-EKPELYETDPSGAYFAWKAVAIGSGRNTAMAIFEEKYKDDMSLEEAI 191          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_THEK1 (Proteasome subunit alpha OS=Thermococcus sp. (strain KS-1) GN=psmA PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 4.930e-33
Identity = 69/172 (40.12%), Postives = 103/172 (59.88%), Query Frame = 3
Query:   57 GYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEK 572
            GYD ++T FSPDGR+FQ+ YA +AV    T +G+K KDG+V+ VEK I SK++ P S  +I  +  H   A +G+ AD R +V RA+ EA  Y   YGEP+P+  L +++       T Y  +RPFG  +++ G + + P+L+  +PSG  + +   AIG GR  A    E+
Sbjct:    9 GYDRAITVFSPDGRLFQVNYAREAVKRGATAVGVKWKDGVVLAVEKRITSKLIEPSSYEKIFLIDDHIAAAPSGIIADARVLVDRARLEAQIYRLTYGEPVPLTVLVKKICDLKQAHTQYGGVRPFGAALLMAGVN-EKPELFETDPSGAYFEWKAVAIGSGRNTAMAIFEE 179          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_PYRKO (Proteasome subunit alpha OS=Pyrococcus kodakaraensis GN=psmA PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 4.930e-33
Identity = 69/172 (40.12%), Postives = 103/172 (59.88%), Query Frame = 3
Query:   57 GYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEK 572
            GYD ++T FSPDGR+FQ+ YA +AV    T +G+K KDG+V+ VEK I SK++ P S  +I  +  H   A +G+ AD R +V RA+ EA  Y   YGEP+P+  L +++       T Y  +RPFG  +++ G + + P+L+  +PSG  + +   AIG GR  A    E+
Sbjct:    9 GYDRAITVFSPDGRLFQVNYAREAVKRGATAVGVKWKDGVVLAVEKRITSKLIEPSSYEKIFLIDDHIAAAPSGIIADARVLVDRARLEAQIYRLTYGEPVPLTVLVKKICDLKQAHTQYGGVRPFGAALLMAGVN-EKPELFETDPSGAYFEWKAVAIGSGRNTAMAIFEE 179          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_THEGJ (Proteasome subunit alpha OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=psmA PE=3 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 8.409e-33
Identity = 72/188 (38.30%), Postives = 110/188 (58.51%), Query Frame = 3
Query:   57 GYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVA 620
            GYD ++T FSPDGR+FQ+ YA +AV    T +G+K K G+V+ VEK I S+++ P S  +I  +  H   A +G+ AD R +V RA+ EA  Y   YGEP+P+  L +++       T Y  +RPFG  +++ G + + P+LY  +PSG  + +   AIG GR  A    E+   S+    +G I++A
Sbjct:    9 GYDRAITVFSPDGRLFQVNYAREAVKRGATAVGVKWKGGVVLAVEKRITSRLIEPSSYEKIFQIDDHIAAAPSGIIADARVLVDRARLEAQIYRLTYGEPVPLTVLVKKICDLKQAHTQYGGVRPFGAALLMAGVN-EKPELYETDPSGAYFEWKAVAIGSGRNTAMAIFEE-HYSDDIDMEGAIKLA 194          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_METM5 (Proteasome subunit alpha OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=psmA PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.873e-32
Identity = 71/185 (38.38%), Postives = 107/185 (57.84%), Query Frame = 3
Query:   54 TGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGV 608
            +GYD ++T FSP+GR++Q+EYA +AV    T +GIKCKDG+V+ V++ I SK++   S  +I  +  H   A +GL AD R ++ RA+ EA      YGE I V+ LA+++       T +   RPFG  +++ G DR   +L+  +PSG    Y   AIG GR  A   +E     +MT  +G+
Sbjct:    8 SGYDRAITIFSPEGRLYQVEYAREAVRRGTTAVGIKCKDGVVLAVDRRITSKLIDVSSIEKIFQIDDHIVAATSGLVADARVLIDRARLEAQMNRISYGEAITVEALAKKICDIKQAYTQHGGARPFGLALLITGIDRHSARLFETDPSGALIEYKATAIGSGRPIAMEVLESKYSEDMTVNEGM 192          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_METJA (Proteasome subunit alpha OS=Methanocaldococcus jannaschii GN=psmA PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.195e-32
Identity = 74/223 (33.18%), Postives = 124/223 (55.61%), Query Frame = 3
Query:   54 TGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDEAKDKAFELEMSWVCDESNRQHQKVP 722
            + YD ++T FSP+GR++Q+EYA +AV    T IGI CKDG+V+ V++ I SK++   S  +I  +  H   A +GL AD R ++ RA+ EA  Y   YGE I ++ LA+++       T +  +RPFG  +++ G D++  +L+  +PSG    Y   AIG GR      +EK    ++T  +G+      +   +++ K +  ++ +  V D    Q +K+P
Sbjct:    7 SAYDRAITVFSPEGRLYQVEYAREAVRRGTTAIGIACKDGVVLAVDRRITSKLVKIRSIEKIFQIDDHVAAATSGLVADARVLIDRARLEAQIYRLTYGEEISIEMLAKKICDIKQAYTQHGGVRPFGVSLLIAGIDKNEARLFETDPSGALIEYKATAIGSGRPVVMELLEKEYRDDITLDEGLELAITALTKANEDIKPENVDVCIITVKD---AQFKKIP 226          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSMA_METM7 (Proteasome subunit alpha OS=Methanococcus maripaludis (strain C7 / ATCC BAA-1331) GN=psmA PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.450e-32
Identity = 71/185 (38.38%), Postives = 106/185 (57.30%), Query Frame = 3
Query:   54 TGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGV 608
            +GYD ++T FSP+GR++Q+EYA +AV    T +GIKCKDG+V+ V++ I SK++   S  +I  +  H   A +GL AD R ++ RA+ EA      YGE I V+ LA+++       T +   RPFG  +++ G DR   +L+  +PSG    Y   AIG GR  A   +E      MT  +G+
Sbjct:    8 SGYDRAITIFSPEGRLYQVEYAREAVRRGTTAVGIKCKDGVVLAVDRRITSKLIDVSSIEKIFQIDDHIVAATSGLVADARVLIDRARLEAQMNRISYGEAITVEALAKKICDIKQAYTQHGGARPFGLALLITGIDRHSARLFETDPSGALIEYKATAIGSGRPIAMEVLESKYDENMTVNEGM 192          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSA5_SOYBN (Proteasome subunit alpha type-5 OS=Glycine max GN=PAE1 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.450e-32
Identity = 75/207 (36.23%), Postives = 114/207 (55.07%), Query Frame = 3
Query:   54 TGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYW---------WLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDE 647
            T YD  V TFSP+GR+FQ+EYA +A+    T IG+K K+G+V+ VEK I S ++ P S  +I  +  H G A++GL AD R +V  A+ E  N+   YGEP+ V+   Q       LC L             RPFG  +++ G+D +GP LY  +PSG  ++  G AIG G + A + +++    ++T ++       I+  V +E
Sbjct:    6 TEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQA------LCDLALRFGEGDEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNGKAIGSGSEGADSSLQEQFNKDLTLQEAETIALSILKQVMEE 206          
BLAST of CX077956 vs. ExPASy Swiss-Prot
Match: PSA5_ORYSJ (Proteasome subunit alpha type-5 OS=Oryza sativa subsp. japonica GN=PAE1 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.450e-32
Identity = 74/207 (35.75%), Postives = 113/207 (54.59%), Query Frame = 3
Query:   54 TGYDLSVTTFSPDGRVFQIEYAAKAVDNSGTVIGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHSGMAVAGLAADGRQIVTRAKSEATNYESVYGEPIPVKELAQRVASYVHLCTLYW---------WLRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGRQAAKTEIEKLKLSEMTCRQGVIEVAKIIYGVHDE 647
            T YD  V TFSP+GR+FQ+EYA +A+    T IG+K KDG+V+ VEK + S ++ P S  +I  +  H G A++GL AD R +V  A+ E  N+   YGEP+ V+   Q +      C L             RPFG  +++ G+D +GP LY  +PSG  ++    AIG G + A + +++    E+T ++       I+  V +E
Sbjct:    6 TEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKDGVVLAVEKRVTSPLLEPSSVEKIMEIDEHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQAI------CDLALRFGEGDEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKELTLQEAETIALSILKQVMEE 206          
The following BLAST results are available for this feature:
BLAST of CX077956 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 176
Match NameE-valueIdentityDescription
PSA5_SCHPO2.890e-3336.49Probable proteasome subunit alpha type-5 OS=Schizo... [more]
PSMA_PYRHO3.775e-3336.41Proteasome subunit alpha OS=Pyrococcus horikoshii ... [more]
PSMA_THEK14.930e-3340.12Proteasome subunit alpha OS=Thermococcus sp. (stra... [more]
PSMA_PYRKO4.930e-3340.12Proteasome subunit alpha OS=Pyrococcus kodakaraens... [more]
PSMA_THEGJ8.409e-3338.30Proteasome subunit alpha OS=Thermococcus gammatole... [more]
PSMA_METM51.873e-3238.38Proteasome subunit alpha OS=Methanococcus maripalu... [more]
PSMA_METJA3.195e-3233.18Proteasome subunit alpha OS=Methanocaldococcus jan... [more]
PSMA_METM75.450e-3238.38Proteasome subunit alpha OS=Methanococcus maripalu... [more]
PSA5_SOYBN5.450e-3236.23Proteasome subunit alpha type-5 OS=Glycine max GN=... [more]
PSA5_ORYSJ5.450e-3235.75Proteasome subunit alpha type-5 OS=Oryza sativa su... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_6A09_b Parent Washington Navel Orange Callus cDNA Library UCRCS08-2 Citrus sinensis cDNA clone UCRCS08-6A09-A18-1-5.b, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX077956 ID=CX077956; Name=CX077956; organism=Citrus sinensis; type=EST; length=860bp
CTTGACTCACAAATCGAAAAAGAAAGAAGAGAGCAAGAATGAGCAGCATA
GGCACAGGCTACGATCTGTCGGTGACCACATTCTCTCCCGACGGTCGCGT
TTTCCAGATCGAATACGCCGCCAAAGCCGTCGACAACAGCGGGACTGTGA
TTGGAATTAAATGCAAAGATGGCATCGTTATGGGAGTGGAGAAGCTAATA
GCGTCGAAGATGATGTTGCCTGGTTCCAATAGAAGAATCCATTCCGTTCA
TCGTCACTCCGGCATGGCTGTTGCAGGATTAGCAGCAGATGGGAGACAAA
TTGTTACCAGGGCCAAGTCTGAAGCTACAAACTATGAAAGTGTATATGGC
GAACCAATTCCTGTTAAAGAACTTGCTCAACGAGTTGCTAGCTATGTGCA
TCTATGTACTCTATATTGGTGGCTCAGACCTTTTGGATGTGGTGTAATTC
TTGGAGGTTATGACAGAGATGGACCGCAATTGTATATGATTGAACCTTCT
GGTATCTCCTATAGGTATTTTGGAGCTGCAATTGGAAAGGGCAGGCAGGC
TGCTAAAACAGAGATTGAAAAGTTGAAGCTTTCTGAAATGACCTGCCGAC
AAGGGGTCATTGAAGTAGCCAAGATCATTTATGGAGTGCACGATGAGGCT
AAGGACAAGGCCTTTGAACTGGAAATGAGCTGGGTCTGTGATGAATCAAA
CCGCCAGCATCAGAAGGTACCAGATGAGCTTCTTGAGGAAGCTAAGGCAG
CCGCCAGAGCAGCGTTGGAAGAGATGGATGCAGATTAGTGTAGCAGAGTT
GATAATATTTGGTTACTAGTTTGATGCCATTTCAGGGCTTGTCTTGGAAT
ACTTTTCGAA
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