DR908520 | Citrus Genome Database

Overview


Name	DR908520
Unique Name	DR908520
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	623

Libraries


Library Name	Type
Citrus sinensis phloem	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.720e-20
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGG 76

HSP 2 Score: 84.7297 bits (208), Expect = 4.064e-16
Identity = 37/67 (55.22%), Postives = 51/67 (76.12%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 203
            I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:   13 IEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 1.763e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = 3

Query:  201 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76

HSP 2 Score: 83.5741 bits (205), Expect = 9.054e-16
Identity = 38/65 (58.46%), Postives = 51/65 (78.46%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 197
            I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:   13 IEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.143e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 78.9518 bits (193), Expect = 2.230e-14
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   13 IEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.143e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 78.9518 bits (193), Expect = 2.230e-14
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   13 IEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.143e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 78.9518 bits (193), Expect = 2.230e-14
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   13 IEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.143e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 78.9518 bits (193), Expect = 2.230e-14
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   13 IEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.143e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 78.9518 bits (193), Expect = 2.230e-14
Identity = 34/64 (53.12%), Postives = 49/64 (76.56%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   13 IEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.492e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 80.4925 bits (197), Expect = 7.665e-15
Identity = 35/64 (54.69%), Postives = 49/64 (76.56%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:   13 IDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 9.674e-18
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 77.411 bits (189), Expect = 6.489e-14
Identity = 34/64 (53.12%), Postives = 48/64 (75.00%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 194
            I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:   13 IDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

BLAST of DR908520 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 2.155e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  195 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 422
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 2.466e-13
Identity = 32/65 (49.23%), Postives = 49/65 (75.38%), Query Frame = 3

Query:    3 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 197
            I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:   13 IEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

The following BLAST results are available for this feature:

BLAST of DR908520 vs. ExPASy Swiss-Prot

BLAST of DR908520 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98


Match Name	E-value	Identity	Description
NEDD8_DROME	2.720e-20	59.21	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]
RUB2_ARATH	1.763e-19	62.16	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	1.143e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	1.143e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	1.143e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	1.143e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	1.143e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	1.492e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	9.674e-18	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	2.155e-17	53.95	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_16648 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-41_G09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR908520 ID=DR908520; Name=DR908520; organism=Citrus sinensis; type=EST; length=623bp
CAATTACTCTTGAGGTTGAAAGTTCTGACACCATCGACAACGTGAAAGCA
AAAATTCAAGACAAGGAGGGTATCCCACCAGATCAGCAGAGGCTCATTTT
TGCTGGAAAGCAGCTTGAAGATGGAAGGACTCTTGCAGACTATAACATCC
AGAAAGAATCCACTCTTCACTTGGTTCTTCGCCTCCGTGGTGGTATGCAG
ATTTTTGTGAAGACACTCACTGGGAAAACCATCACATTGGAGGTTGAGAG
TTCGGACACAATTGACAATGTGAAGGCTAAGATCCAGGATAAGGAGGGGA
TTCCCCCAGACCAGCAGAGGTTGATCTTTGCTGGAAAACAACTTGAGGAT
GGAAGGACTCTTGCTGATTACAACATCCAGAAGGAGTCCACTCTGCATCT
TGTGCTCCGTCTCCGTGGAGGTTTCTGAGTTTGGTCATCTGGGTGTGTGT
GTGTCTTATTTGCTTCGGTTTGTCTTGGTTAATGTTTGCTTGCTATTGGT
GAAATGGCTTAAATAACTATTTGAAGTTTATTTTGGTTGTGGTAGTTGAA
CTCTGTTTCTAAGATATTGATATGGTCCCACTAACGTCCCATTATAATAT
CTAAGAATCTTTTTCTTGAATAA