DR908327

Overview
NameDR908327
Unique NameDR908327
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length665
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACP2 (S-adenosylmethionine synthetase OS=Bacillus pumilus (strain SAFR-032) GN=metK PE=3 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 3.363e-58
Identity = 100/180 (55.56%), Postives = 136/180 (75.56%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY ++N    PVR+ TV+ISTQH   VT ++I +DLK+HVI PV+P + +D++T + +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA  C VQ++YAIGV +P+S+ +D++GTGK  +++++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEYDDQNK---PVRIDTVVISTQHAPEVTLEQIQSDLKEHVISPVVPSELIDEQTKYFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLADSCEVQLAYAIGVAQPVSISIDTFGTGKASEEKLIEVVRANFD 349

HSP 2 Score: 38.1206 bits (87), Expect = 3.363e-58
Identity = 22/47 (46.81%), Postives = 25/47 (53.19%), Query Frame = 2 
Query:  521 EDCEGEF*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            E     F  RP  I   LDL+R     + +TAAYGHFGR D D  WE
Sbjct:  342 EVVRANFDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRHDLDLPWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACHK (S-adenosylmethionine synthetase OS=Bacillus thuringiensis subsp. konkukian GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 5.717e-58
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 40.4318 bits (93), Expect = 5.717e-58
Identity = 22/41 (53.66%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D TWE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLTWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACC7 (S-adenosylmethionine synthetase OS=Bacillus cereus (strain AH187) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 5.717e-58
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 40.4318 bits (93), Expect = 5.717e-58
Identity = 22/41 (53.66%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D TWE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLTWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACCZ (S-adenosylmethionine synthetase OS=Bacillus cereus (strain ZK / E33L) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACCR (S-adenosylmethionine synthetase OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELMDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACCQ (S-adenosylmethionine synthetase OS=Bacillus cereus (strain Q1) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACC4 (S-adenosylmethionine synthetase OS=Bacillus cereus (strain B4264) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELMDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACC3 (S-adenosylmethionine synthetase OS=Bacillus cereus (strain 03BB102) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACC1 (S-adenosylmethionine synthetase OS=Bacillus cereus (strain ATCC 10987) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
[Close]
BLAST of DR908327 vs. ExPASy Swiss-Prot
Match: METK_BACC0 (S-adenosylmethionine synthetase OS=Bacillus cereus (strain AH820) GN=metK PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.652e-57
Identity = 104/180 (57.78%), Postives = 134/180 (74.44%), Query Frame = 3 
Query:    6 WLRPDGKTQVTVEYYNENGAMVPVRVHTVLISTQHDETVTNDEIAADLKQHVIKPVIPDKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFD 545
            +LRPDGKTQVTVEY +ENG   PVRV T++ISTQH   VT +EI  DLK+HVIK V+P + +D +T F +NP+GRFVIGGP GDAGLTGRKII+DTY             KD TKVDRS AY  R  AK+IVA+GLA +  VQ++YAIGV +P+S+ VD++GTGK+ +  ++++V+ NFD
Sbjct:  173 YLRPDGKTQVTVEY-DENGK--PVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFD 349

HSP 2 Score: 38.891 bits (89), Expect = 1.652e-57
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2 
Query:  539 F*FRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 661
            F  RP  I   LDL+R     + +TAAYGHFGR D D +WE
Sbjct:  348 FDLRPAGIIKMLDLRRP---IYKQTAAYGHFGRTDVDLSWE 385
The following BLAST results are available for this feature:
BLAST of DR908327 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_BACP23.363e-5855.56S-adenosylmethionine synthetase OS=Bacillus pumilu... [more]
METK_BACHK5.717e-5857.78S-adenosylmethionine synthetase OS=Bacillus thurin... [more]
METK_BACC75.717e-5857.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACCZ1.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACCR1.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACCQ1.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACC41.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACC31.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACC11.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]
METK_BACC01.652e-5757.78S-adenosylmethionine synthetase OS=Bacillus cereus... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_16455 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-10_H05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR908327 ID=DR908327; Name=DR908327; organism=Citrus sinensis; type=EST; length=665bp
GTCCGTGGCTGAGGCCAGATGGCAAAACACAAGTCACTGTTGAGTATTAC
AATGAGAATGGCGCCATGGTTCCTGTTCGTGTCCATACTGTGCTCATCTC
AACTCAGCACGATGAGACTGTAACTAATGATGAGATTGCTGCAGATCTCA
AGCAACATGTGATCAAGCCTGTGATTCCTGACAAGTACTTGGATGACAAA
ACTATTTTCCATCTTAACCCATCTGGCCGTTTTGTGATTGGTGGCCCTCA
CGGTGATGCTGGGCTCACCGGCCGGAAGATTATTATTGATACTTATGGTG
GCTGGGGGGCACACGGTGGTGGGGCTTTCTCAGGGAAAGATCCTACCAAG
GTTGATAGAAGCGGTGCTTACATTGTAAGGCAAGCTGCTAAGAGTATTGT
GGCAAGCGGGCTTGCCAGGAGATGCATTGTTCAGGTTTCTTACGCTATTG
GTGTGCCCGAGCCTTTGTCAGTTTTTGTTGATAGTTATGGGACTGGGAAG
ATCCCTGACAAGGAAATTTTGAAGATTGTGAAGGAGAATTTTGATTTAGG
CCTGGGATGATTTCTATTAATCTTGATCTCAAGAGGGGTGGCAACGGCAG
GTTCTTGAAGACTGCCGCTTATGGGCACTTTGGCAGAGATGATCCTGATT
TCACTTGGGAGATTG
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