EY701063

Overview
NameEY701063
Unique NameEY701063
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1056
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: LATS2_MOUSE (Serine/threonine-protein kinase LATS2 OS=Mus musculus GN=Lats2 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 9.392e-19
Identity = 43/73 (58.90%), Postives = 59/73 (80.82%), Query Frame = 2 
Query:  104 VEHVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIESI 322
            V HV+AER++LAE  +  +VKLYYSFQD + LY +M+Y+PGGDMM+LL+R +   E++ARFYIA+  LAIES+
Sbjct:  668 VAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESV 740
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: LATS2_HUMAN (Serine/threonine-protein kinase LATS2 OS=Homo sapiens GN=LATS2 PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 9.392e-19
Identity = 43/73 (58.90%), Postives = 59/73 (80.82%), Query Frame = 2 
Query:  104 VEHVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIESI 322
            V HV+AER++LAE  +  +VKLYYSFQD + LY +M+Y+PGGDMM+LL+R +   E++ARFYIA+  LAIES+
Sbjct:  710 VAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESV 782
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: LATS1_MOUSE (Serine/threonine-protein kinase LATS1 OS=Mus musculus GN=Lats1 PE=1 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 7.950e-18
Identity = 41/73 (56.16%), Postives = 57/73 (78.08%), Query Frame = 2 
Query:  104 VEHVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIESI 322
            V HV+AER++LAE  +  +V+LYYSFQD + LY +M+Y+PGGDMM+LL+R     EN+ARFYIA+   A+ES+
Sbjct:  746 VAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPENLARFYIAELTCAVESV 818
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: PKGA_DICDI (Probable serine/threonine-protein kinase pkgA OS=Dictyostelium discoideum GN=pkgA PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.063e-17
Identity = 36/71 (50.70%), Postives = 53/71 (74.65%), Query Frame = 2 
Query:  104 VEHVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIE 316
            ++HV+ ERN+LA  ++  +VK+YYSFQ  EY YL+MEYL GGD  +LL    ++ EN+A+  IA++VLA+E
Sbjct:  852 LDHVKVERNILAYTSNPFVVKMYYSFQTKEYYYLVMEYLQGGDCFSLLQMLGSMDENMAKMIIAETVLALE 922

HSP 2 Score: 30.8018 bits (68), Expect = 2.063e-17
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  312 LSLSHQHSYMHRDIRPDDLLLGPK 383
            L   H H  +HRD++PD+LL+  K
Sbjct:  921 LEYLHSHGIVHRDVKPDNLLIDKK 944
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: LATS1_HUMAN (Serine/threonine-protein kinase LATS1 OS=Homo sapiens GN=LATS1 PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.021e-17
Identity = 40/73 (54.79%), Postives = 57/73 (78.08%), Query Frame = 2 
Query:  104 VEHVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIESI 322
            V HV+AER++LAE  +  +V+LYYSFQD + LY +M+Y+PGGDMM+LL+R     E++ARFYIA+   A+ES+
Sbjct:  747 VAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESV 819
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: COT1_NEUCR (Serine/threonine-protein kinase cot-1 OS=Neurospora crassa GN=cot-1 PE=2 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 3.946e-17
Identity = 39/71 (54.93%), Postives = 59/71 (83.10%), Query Frame = 2 
Query:  110 HVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIESI 322
            HVRAER++LAE  S  +VKLY +FQD  +LY++ME+LPGGD+MT+L++ +  +E++ RFYIA+ VLAI+++
Sbjct:  258 HVRAERDILAESDSPWVVKLYTTFQDANFLYMLMEFLPGGDLMTMLIKYEIFSEDITRFYIAEIVLAIDAV 328
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: MRCKB_RAT (Serine/threonine-protein kinase MRCK beta OS=Rattus norvegicus GN=Cdc42bpb PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 3.879e-15
Identity = 37/70 (52.86%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  116 RAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMR-EDTLTENVARFYIAQSVLAIESI 322
            R ER++L       I  L+Y+FQD  YLYL+M+Y  GGD++TLL + ED L E++ARFYI + VLAI+SI
Sbjct:  122 REERDVLVNGDCQWITALHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSI 191

HSP 2 Score: 29.6462 bits (65), Expect = 3.879e-15
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:  324 HQHSYMHRDIRPDDLLL 374
            HQ  Y+HRDI+PD++LL
Sbjct:  192 HQLHYVHRDIKPDNVLL 208
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: MRCKB_MOUSE (Serine/threonine-protein kinase MRCK beta OS=Mus musculus GN=Cdc42bpb PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 3.879e-15
Identity = 37/70 (52.86%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  116 RAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMR-EDTLTENVARFYIAQSVLAIESI 322
            R ER++L       I  L+Y+FQD  YLYL+M+Y  GGD++TLL + ED L E++ARFYI + VLAI+SI
Sbjct:  122 REERDVLVNGDCQWITALHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSI 191

HSP 2 Score: 29.6462 bits (65), Expect = 3.879e-15
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:  324 HQHSYMHRDIRPDDLLL 374
            HQ  Y+HRDI+PD++LL
Sbjct:  192 HQLHYVHRDIKPDNVLL 208
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: Y1558_DICDI (Probable serine/threonine-protein kinase DDB_G0278845 OS=Dictyostelium discoideum GN=DDB_G0278845 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 8.549e-15
Identity = 38/73 (52.05%), Postives = 55/73 (75.34%), Query Frame = 2 
Query:  104 VEHVRAERNLLAEVASH-CIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMREDTLTENVARFYIAQSVLAIES 319
            V H++ ER++LA+  +H  IV L YSFQD +YLYL MEY+PGGD+ +LL   ++L E+ ARFY+A+ V A++S
Sbjct:  798 VSHIKNERDILAQGKNHRWIVSLVYSFQDDQYLYLAMEYVPGGDLRSLLSALNSLDEDSARFYMAEMVEAVDS 870

HSP 2 Score: 24.2534 bits (51), Expect = 8.549e-15
Identity = 7/17 (41.18%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:  324 HQHSYMHRDIRPDDLLL 374
            H+  Y HRD++P++ L+
Sbjct:  872 HRLGYCHRDLKPENFLI 888
[Close]
BLAST of EY701063 vs. ExPASy Swiss-Prot
Match: MRCKB_HUMAN (Serine/threonine-protein kinase MRCK beta OS=Homo sapiens GN=CDC42BPB PE=1 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 1.436e-14
Identity = 36/70 (51.43%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  116 RAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDMMTLLMR-EDTLTENVARFYIAQSVLAIESI 322
            R ER++L       I  L+Y+FQD  +LYL+M+Y  GGD++TLL + ED L E++ARFYI + VLAI+SI
Sbjct:  122 REERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSI 191

HSP 2 Score: 29.6462 bits (65), Expect = 1.436e-14
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:  324 HQHSYMHRDIRPDDLLL 374
            HQ  Y+HRDI+PD++LL
Sbjct:  192 HQLHYVHRDIKPDNVLL 208
The following BLAST results are available for this feature:
BLAST of EY701063 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 49
Match NameE-valueIdentityDescription
LATS2_MOUSE9.392e-1958.90Serine/threonine-protein kinase LATS2 OS=Mus muscu... [more]
LATS2_HUMAN9.392e-1958.90Serine/threonine-protein kinase LATS2 OS=Homo sapi... [more]
LATS1_MOUSE7.950e-1856.16Serine/threonine-protein kinase LATS1 OS=Mus muscu... [more]
PKGA_DICDI2.063e-1750.70Probable serine/threonine-protein kinase pkgA OS=D... [more]
LATS1_HUMAN3.021e-1754.79Serine/threonine-protein kinase LATS1 OS=Homo sapi... [more]
COT1_NEUCR3.946e-1754.93Serine/threonine-protein kinase cot-1 OS=Neurospor... [more]
MRCKB_RAT3.879e-1552.86Serine/threonine-protein kinase MRCK beta OS=Rattu... [more]
MRCKB_MOUSE3.879e-1552.86Serine/threonine-protein kinase MRCK beta OS=Mus m... [more]
Y1558_DICDI8.549e-1552.05Probable serine/threonine-protein kinase DDB_G0278... [more]
MRCKB_HUMAN1.436e-1451.43Serine/threonine-protein kinase MRCK beta OS=Homo ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-102-F01-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY701063 ID=EY701063; Name=EY701063; organism=Citrus sinensis; type=EST; length=1056bp
CCCGGAGTGGACTAACTGTTCACACATTATTACTGTAGTGTCATTTGCAT
TTGCAGTTTGTGCTGGTTTATTAGTGCGGATAAGATGCCTTTCTGCTTTG
TAGGTAGAACATGTAAGAGCTGAGAGGAACTTACTGGCTGAGGTTGCGAG
TCATTGCATAGTGAAGCTCTACTACTCATTCCAGGATACTGAGTACCTAT
ATTTGATTATGGAATACCTACCTGGTGGCGATATGATGACTTTGTTGATG
AGGGAGGACACCTTAACTGAAAACGTGGCTAGGTTTTACATTGCCCAAAG
TGTCCTGGCTATTGAGTCTATCCCACCAACATAGCTACATGCACAGAGAT
ATTAGACCTGACGACCTTCTTCTGGGACCAAAATGGTCATATGAAGGTTA
TCAGATGTTGGGCCTTTGCCAAGCCTCTTGGACTGGGTCAACCTTTATTT
GCAATCGCATGAGCATTAGAGCCGGTGGATGGATGAGAAAGTATGGGCGC
GCCCCGATGGGATCTTCGAGTGGGATGCCTTTTCCTTGACCACGGGGTGG
TGGAGGAGGTGGTGGGGGGAGAGGGGCCCCGGTGGAGCCGGCGTTTCCAC
GCGATGTGGGGGGGAGGTGGGCGGCGGGGGGGAGAAGGTCGGGCATTTTT
CTCCGGAGTGGGGTGGGGGAACACGATCGGAAGCCTGCGGTTCGCGCGCC
CGTGGGGCGGTGGTGGTGCTCGCGGCGAAGACGGGACCGCGCTGCCTCGG
GCCACGCGCGGCGCGGAGGGGGGTCCCGCGGCGGCGCGCCCCGGGCGTGC
TCCGGGCGGCCGGCCCCGGCGGCGCCCGCCGCCCCCCGCGGGTGGGCGGG
GGACGAGGGGCGGCCCCGGCGGGAGCCTGGTCTTTGTGTGTGAGTGTGGG
GGCGGGGGGGCAGAGGGCCGCGGGTCGTCGNNCCGGACGGGGGAGGGGAG
AGCCTCGGGGGCCGCCGGCCCCCGCCGCGCGGGGCCCACCGCGCGAGCGC
TTGGGAGTCGCGAAGGGAGTGAAAACGCGCGGTTGCCGCTCCGCGNATGG
CGCCAC
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