EY701055

Overview
NameEY701055
Unique NameEY701055
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length957
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_CORA7 (Elongation factor Tu OS=Corynebacterium aurimucosum (strain ATCC 700975 / DSM 44827 / CN-1) GN=tuf PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 7.109e-12
Identity = 32/88 (36.36%), Postives = 50/88 (56.82%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            H+NI  IGHVD GK+TTT         I K + + + +E       +F +  ++DK   E+ERGITI+I+  ++ T + +   +DAPG
Sbjct:   12 HVNIGTIGHVDHGKTTTTA-------AITKVLADAYPEE-----NTAFAFD-MIDKAPEEKERGITINISHVEYSTPKRHYAHVDAPG 86

HSP 2 Score: 39.6614 bits (91), Expect = 7.109e-12
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++ +T G
Sbjct:   86 GHADYIKNMITGAAQMDGAILVVAATDG 113
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_ARATH (Elongation factor Tu, chloroplastic OS=Arabidopsis thaliana GN=TUFA PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.547e-11
Identity = 30/92 (32.61%), Postives = 45/92 (48.91%), Query Frame = 1 
Query:  139 KENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            ++  H+NI  IGHVD GK+T T  L   L  I   V +++++               +D    ER RGITI+ A  ++ET   +   +D PG
Sbjct:   75 RKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE---------------IDAAPEERARGITINTATVEYETENRHYAHVDCPG 151

HSP 2 Score: 37.7354 bits (86), Expect = 1.547e-11
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++    G
Sbjct:  151 GHADYVKNMITGAAQMDGAILVVSGADG 178
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_LACS1 (Elongation factor Tu OS=Lactobacillus salivarius subsp. salivarius (strain UCC118) GN=tuf PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.556e-11
Identity = 36/88 (40.91%), Postives = 48/88 (54.55%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            H+NI  IGHVD GK+T T         I K + E+   EA++       YA + D    ERERGITI+ A  ++ET + +   IDAPG
Sbjct:   12 HVNIGTIGHVDHGKTTLTA-------AITKVLAEKGLAEASD-------YASI-DAAPEERERGITINTAHVEYETEKRHYAHIDAPG 84

HSP 2 Score: 39.2762 bits (90), Expect = 1.556e-11
Identity = 15/28 (53.57%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++ +T G
Sbjct:   84 GHADYVKNMITGAAQMDGAILVVAATDG 111
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_YEAST (Elongation factor Tu, mitochondrial OS=Saccharomyces cerevisiae GN=TUF1 PE=1 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 2.613e-11
Identity = 33/98 (33.67%), Postives = 45/98 (45.92%), Query Frame = 1 
Query:  121 YICIMGKENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            Y     +   H+NI  IGHVD GK+T T  +   L              AA+       YA  +DK   ER RGITI  A  ++ET + + + +D PG
Sbjct:   38 YAAAFDRSKPHVNIGTIGHVDHGKTTLTAAITKTL--------------AAKGGANFLDYA-AIDKAPEERARGITISTAHVEYETAKRHYSHVDCPG 120

HSP 2 Score: 38.891 bits (89), Expect = 2.613e-11
Identity = 15/28 (53.57%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A++++ +T G
Sbjct:  120 GHADYIKNMITGAAQMDGAIIVVAATDG 147
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_STRSV (Elongation factor Tu OS=Streptococcus sanguinis (strain SK36) GN=tuf PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 2.621e-11
Identity = 35/92 (38.04%), Postives = 46/92 (50.00%), Query Frame = 1 
Query:  139 KENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            +   H+NI  IGHVD GK+T T  +          V+ R    A    K    YA + D    ERERGITI+ A  ++ET + +   IDAPG
Sbjct:    8 RSKPHVNIGTIGHVDHGKTTLTAAIT--------TVLARRLPSAVNQPK---DYASI-DAAPEERERGITINTAHVEYETAKRHYAHIDAPG 87

HSP 2 Score: 40.4318 bits (93), Expect = 2.621e-11
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++ ST G
Sbjct:   87 GHADYVKNMITGAAQMDGAILVVASTDG 114
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_SYMTH (Elongation factor Tu OS=Symbiobacterium thermophilum GN=tuf PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.622e-11
Identity = 32/88 (36.36%), Postives = 42/88 (47.73%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            H+NI  IGHVD GK+T T  +                K  AE  K  F     +DK   ERERGITI+ +  ++ET   +   +D PG
Sbjct:   12 HVNIGTIGHVDHGKTTLTAAIT---------------KVLAEKGKAQFMAYDAIDKAPEERERGITINTSHVEYETDARHYAHVDCPG 84

HSP 2 Score: 38.1206 bits (87), Expect = 2.622e-11
Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++ +  G
Sbjct:   84 GHADYVKNMITGAAQMDGAILVVSAADG 111
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_EHRRW (Elongation factor Tu OS=Ehrlichia ruminantium (strain Welgevonden) GN=tuf1 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 2.622e-11
Identity = 32/88 (36.36%), Postives = 42/88 (47.73%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            HIN+  IGHVD GK+T T  L   L            K  +    +S KY   +DK   E+ RGITI  A  ++ET   +   +D PG
Sbjct:    8 HINVGTIGHVDHGKTTLTAALTTVLA-----------KRLSGEGNKSVKYD-EIDKAPEEKARGITISTAHVEYETENRHYAHVDCPG 83

HSP 2 Score: 41.5874 bits (96), Expect = 2.622e-11
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++ +T G
Sbjct:   83 GHADYIKNMITGAAQMDAAILVVSATDG 110
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_EHRRG (Elongation factor Tu OS=Ehrlichia ruminantium (strain Gardel) GN=tuf1 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 2.622e-11
Identity = 32/88 (36.36%), Postives = 42/88 (47.73%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            HIN+  IGHVD GK+T T  L   L            K  +    +S KY   +DK   E+ RGITI  A  ++ET   +   +D PG
Sbjct:    8 HINVGTIGHVDHGKTTLTAALTTVLA-----------KRLSGEGNKSVKYD-EIDKAPEEKARGITISTAHVEYETENRHYAHVDCPG 83

HSP 2 Score: 41.5874 bits (96), Expect = 2.622e-11
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++ +T G
Sbjct:   83 GHADYIKNMITGAAQMDAAILVVSATDG 110
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_EHRCR (Elongation factor Tu OS=Ehrlichia chaffeensis (strain Arkansas) GN=tuf1 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 2.622e-11
Identity = 32/88 (36.36%), Postives = 42/88 (47.73%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            HIN+  IGHVD GK+T T  L   L            K  +    +S KY   +DK   E+ RGITI  A  ++ET   +   +D PG
Sbjct:    8 HINVGTIGHVDHGKTTLTAALTTVLA-----------KRLSGEGNKSVKYD-EIDKAPEEKARGITISTAHVEYETENRHYAHVDCPG 83

HSP 2 Score: 41.5874 bits (96), Expect = 2.622e-11
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++ +T G
Sbjct:   83 GHADYIKNMITGAAQMDAAILVVSATDG 110
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_EHRCJ (Elongation factor Tu OS=Ehrlichia canis (strain Jake) GN=tuf1 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 2.622e-11
Identity = 32/88 (36.36%), Postives = 42/88 (47.73%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            HIN+  IGHVD GK+T T  L   L            K  +    +S KY   +DK   E+ RGITI  A  ++ET   +   +D PG
Sbjct:    8 HINVGTIGHVDHGKTTLTAALTTVLA-----------KRLSGEGNKSVKYD-EIDKAPEEKARGITISTAHVEYETENRHYAHVDCPG 83

HSP 2 Score: 41.5874 bits (96), Expect = 2.622e-11
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++ +T G
Sbjct:   83 GHADYIKNMITGAAQMDAAILVVSATDG 110
The following BLAST results are available for this feature:
BLAST of EY701055 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 279
Match NameE-valueIdentityDescription
EFTU_CORA77.109e-1236.36Elongation factor Tu OS=Corynebacterium aurimucosu... [more]
EFTU_ARATH1.547e-1132.61Elongation factor Tu, chloroplastic OS=Arabidopsis... [more]
EFTU_LACS11.556e-1140.91Elongation factor Tu OS=Lactobacillus salivarius s... [more]
EFTU_YEAST2.613e-1133.67Elongation factor Tu, mitochondrial OS=Saccharomyc... [more]
EFTU_STRSV2.621e-1138.04Elongation factor Tu OS=Streptococcus sanguinis (s... [more]
EFTU_SYMTH2.622e-1136.36Elongation factor Tu OS=Symbiobacterium thermophil... [more]
EFTU_EHRRW2.622e-1136.36Elongation factor Tu OS=Ehrlichia ruminantium (str... [more]
EFTU_EHRRG2.622e-1136.36Elongation factor Tu OS=Ehrlichia ruminantium (str... [more]
EFTU_EHRCR2.622e-1136.36Elongation factor Tu OS=Ehrlichia chaffeensis (str... [more]
EFTU_EHRCJ2.622e-1136.36Elongation factor Tu OS=Ehrlichia canis (strain Ja... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-102-E02-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY701055 ID=EY701055; Name=EY701055; organism=Citrus sinensis; type=EST; length=957bp
TTGCGGCCGCGCCGGCCCCCCAACNTGTGTTCNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGAACCATTTTCGCGAATAT
CATTTTAATAAGGTTTTTTCTACATTTGCATAATGGGTAAGGAGAACACT
CACATCAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCAC
CACCGGACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCG
AGAGGTTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATAC
GCCTGGGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCAT
TGATATTGCCTTGTGGAAGTTCGAGACCACCAGGTACTACTGCACCGTCA
TTGATGCTCCTGGGACATCGTGACTTTATCAAGAACATGATTACCGGTAC
CTCACAGGCTGACTGTGCCGTCCTTATCATTGACTCTACCACCGGTGGTT
TTGAAGCTGGTATCTCCCGGGCAGGGTCAGACTCCTGGACCATGCTCGTT
GCTTGGCTTCCACCCTTGGGTGTGGAAGCCAGACTGAGGTGGGCTGGCGG
GTACGGACGGATGGATTGCCGGGCGACCCCCCAAGGTACGCCACAGGCGG
TACGCGTCCGACGCAAAATTTCGTGAAAGGGAGCGTCCTCCTTTCCGTGC
CCTTGAAGGAACCGGCGTTGGAGTGCCCCCCCCCCGCAGCACATTTCTCC
CCTTCCGTGGCCCCCACCTCCCGCGGTTTGCAGGGCGTTACCAGCCTGCA
GTCGCTTAAGTGGCCCACCCGGCGTTCGGCCGGGGGTTAGGGGGGGGGCG
TGGGGGGGGGGGGGGGGGCCGCGCACGCCGCGCCGCCCCGCCCCGGAGGG
GGCGGGCCCGCCCGGCCCGCCGCGCGGGCTGGCCGGCCCCCCCCCCCAGC
GCCGGCC
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