EY701055

Overview
NameEY701055
Unique NameEY701055
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length957
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_LACSS (Elongation factor Tu OS=Lactobacillus sakei subsp. sakei (strain 23K) GN=tuf PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 7.442e-11
Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            H+NI  IGHVD GK+T T         I K + ++   EA +           +D    ERERGITI+ A  ++ET   +   IDAPG
Sbjct:   13 HVNIGTIGHVDHGKTTLTA-------AITKMLADKGLAEAQDYAN--------IDAAPEERERGITINTAHVEYETENRHYAHIDAPG 85

HSP 2 Score: 39.2762 bits (90), Expect = 7.442e-11
Identity = 15/28 (53.57%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++ +T G
Sbjct:   85 GHADYVKNMITGAAQMDGAILVVAATDG 112
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_MICLU (Elongation factor Tu OS=Micrococcus luteus GN=tuf PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 7.442e-11
Identity = 31/93 (33.33%), Postives = 51/93 (54.84%), Query Frame = 1 
Query:  139 KENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNK-RSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            +   H+NI  IGHVD GK+T T         I K + +++     ++N+ R F     +D    ER+RGITI+I+  +++T + +   +DAPG
Sbjct:    8 RTKAHVNIGTIGHVDHGKTTLTA-------AISKVLYDKYP----DLNEARDFA---TIDSAPEERQRGITINISHVEYQTEKRHYAHVDAPG 86

HSP 2 Score: 39.6614 bits (91), Expect = 7.442e-11
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++ +T G
Sbjct:   86 GHADYIKNMITGAAQMDGAILVVAATDG 113
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_CHLPB (Elongation factor Tu OS=Chlorobium phaeobacteroides (strain BS1) GN=tuf PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 7.444e-11
Identity = 32/92 (34.78%), Postives = 44/92 (47.83%), Query Frame = 1 
Query:  139 KENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            ++  H+NI  IGHVD GK+T T  +   L             +A     R F     +DK   ERERGITI  A  +++T + +   ID PG
Sbjct:    8 RDKPHVNIGTIGHVDHGKTTLTAAITSVLA------------KAGNAEMREFGD---IDKAPEERERGITISTAHVEYQTEKRHYAHIDCPG 84

HSP 2 Score: 39.2762 bits (90), Expect = 7.444e-11
Identity = 16/28 (57.14%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A+L++  T G
Sbjct:   84 GHADYIKNMITGAAQMDGAILVVAGTDG 111
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: NODQ_RHITR (Bifunctional enzyme nodQ OS=Rhizobium tropici GN=nodQ PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 9.519e-11
Identity = 34/95 (35.79%), Postives = 50/95 (52.63%), Query Frame = 1 
Query:  154 INIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEM--------NKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            +  +  G VD GKST  G L+      D +++  FE + A++        N     ++ VLD L+AERE+GITID+A   F T++    V D PG
Sbjct:   25 LRFITCGSVDDGKSTLIGRLL-----CDAQLV--FEDQLADLRHGGIRGGNGEEIDFSLVLDGLEAEREQGITIDVAYRYFSTSKRKFIVADTPG 112

HSP 2 Score: 35.039 bits (79), Expect = 9.519e-11
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH ++ +NM TG S AD AV+++DS  G
Sbjct:  112 GHFEYTRNMATGASTADLAVILVDSRQG 139
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: CYSN_RHIME (Sulfate adenylyltransferase subunit 1 OS=Rhizobium meliloti GN=cysN PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 9.588e-11
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 1 
Query:  139 KENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAA-EMNKRSFK-YAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            ++   + ++  G VD GKST  G L++    + +      +++++ + N      +A +LD L+AERE+GITID+A   F T +    V D PG
Sbjct:   28 RDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKEDQAASLQRDSSGKQNDLGLPDFALLLDGLQAEREQGITIDVAYRYFATDKRSFIVADTPG 121

HSP 2 Score: 35.8094 bits (81), Expect = 9.588e-11
Identity = 15/31 (48.39%), Postives = 20/31 (64.52%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTGGFE 505
            GH  + +NM TG S AD AVL++D+  G  E
Sbjct:  121 GHEQYTRNMATGASTADLAVLLVDARVGLLE 151
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: CYSN_SHEWM (Sulfate adenylyltransferase subunit 1 OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=cysN PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 9.605e-11
Identity = 32/89 (35.96%), Postives = 49/89 (55.06%), Query Frame = 1 
Query:  154 INIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKR--SFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            + ++  G VD GKST  G L++    I +  +   EK++   N    SF  A ++D L++ERE+GITID+A   F T +    + D PG
Sbjct:   25 LRVLTCGSVDDGKSTLIGRLLFDSKMIFEDQMAAIEKDSKRFNTTDDSFDLALLVDGLQSEREQGITIDVAYRYFTTEQRKFIIADTPG 113

HSP 2 Score: 32.3426 bits (72), Expect = 9.605e-11
Identity = 12/28 (42.86%), Postives = 18/28 (64.29%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH  + +NM TG S  D A+++ID+  G
Sbjct:  113 GHEQYTRNMATGASTCDLAIILIDARHG 140
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_NEOSM (Elongation factor Tu OS=Neorickettsia sennetsu (strain Miyayama) GN=tuf PE=3 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 9.632e-11
Identity = 30/91 (32.97%), Postives = 43/91 (47.25%), Query Frame = 1 
Query:  142 ENTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            +  H+NI  IGHVD GK+T T              I +F  E     +  F+    +DK   ER+RGITI  A  +++T   +   +D PG
Sbjct:   12 DRPHLNIGTIGHVDHGKTTLTA------------AITKFCSEEGGGYEADFRAYDNIDKAPEERQRGITISTAHVEYKTPERHYAHVDCPG 90

HSP 2 Score: 40.0466 bits (92), Expect = 9.632e-11
Identity = 16/31 (51.61%), Postives = 22/31 (70.97%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTGGFE 505
            GH D+IKNMITG +Q D A+L++  T G  +
Sbjct:   90 GHADYIKNMITGAAQMDGAILVVAGTDGAMQ 120
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_DESRM (Elongation factor Tu OS=Desulfotomaculum reducens (strain MI-1) GN=tuf PE=3 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 9.655e-11
Identity = 29/88 (32.95%), Postives = 43/88 (48.86%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            H+NI  IGHVD GK+T T  +   L       ++R+++               +D    ERERGITI+ A  ++ET   +   +D PG
Sbjct:   12 HVNIGTIGHVDHGKTTLTAAITVVLSTSGGASVKRYDE---------------IDNAPEERERGITINTAHVEYETANRHYAHVDCPG 84

HSP 2 Score: 38.1206 bits (87), Expect = 9.655e-11
Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++ +  G
Sbjct:   84 GHADYVKNMITGAAQMDGAILVVSAADG 111
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_CLOAB (Elongation factor Tu OS=Clostridium acetobutylicum GN=tuf PE=3 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 9.657e-11
Identity = 32/88 (36.36%), Postives = 44/88 (50.00%), Query Frame = 1 
Query:  151 HINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            H+NI  IGHVD GK+T T  +   L              A E   ++F Y  + DK   E+ERGITI+ A  ++ET   +   +D PG
Sbjct:   12 HVNIGTIGHVDHGKTTLTAAITTIL--------------AKEGKAKAFNYEEI-DKAPEEKERGITINTAHVEYETENRHYAHVDCPG 84

HSP 2 Score: 38.1206 bits (87), Expect = 9.657e-11
Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D++KNMITG +Q D A+L++ +  G
Sbjct:   84 GHADYVKNMITGAAQMDGAILVVSAADG 111
[Close]
BLAST of EY701055 vs. ExPASy Swiss-Prot
Match: EFTU_DESDG (Elongation factor Tu OS=Desulfovibrio desulfuricans (strain G20) GN=tuf PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 9.657e-11
Identity = 35/99 (35.35%), Postives = 49/99 (49.49%), Query Frame = 1 
Query:  133 MGKEN-----THINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCTVIDAPG 414
            MGKE       H+NI  IGH+D GK+T T   I K+  +             +M  ++  Y  + DK   E+ERGITI  A  ++ETT  +   +D PG
Sbjct:    1 MGKEKFERTKPHVNIGTIGHIDHGKTTLTA-AITKIASL-------------KMGSKAVAYDEI-DKAPEEKERGITIATAHVEYETTLRHYAHVDCPG 84

HSP 2 Score: 38.891 bits (89), Expect = 9.657e-11
Identity = 15/28 (53.57%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  413 GHRDFIKNMITGTSQADCAVLIIDSTTG 496
            GH D+IKNMITG +Q D A++++ +T G
Sbjct:   84 GHADYIKNMITGAAQMDGAIIVVAATDG 111
The following BLAST results are available for this feature:
BLAST of EY701055 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 279
Match NameE-valueIdentityDescription
EFTU_LACSS7.442e-1137.50Elongation factor Tu OS=Lactobacillus sakei subsp.... [more]
EFTU_MICLU7.442e-1133.33Elongation factor Tu OS=Micrococcus luteus GN=tuf ... [more]
EFTU_CHLPB7.444e-1134.78Elongation factor Tu OS=Chlorobium phaeobacteroide... [more]
NODQ_RHITR9.519e-1135.79Bifunctional enzyme nodQ OS=Rhizobium tropici GN=n... [more]
CYSN_RHIME9.588e-1131.91Sulfate adenylyltransferase subunit 1 OS=Rhizobium... [more]
CYSN_SHEWM9.605e-1135.96Sulfate adenylyltransferase subunit 1 OS=Shewanell... [more]
EFTU_NEOSM9.632e-1132.97Elongation factor Tu OS=Neorickettsia sennetsu (st... [more]
EFTU_DESRM9.655e-1132.95Elongation factor Tu OS=Desulfotomaculum reducens ... [more]
EFTU_CLOAB9.657e-1136.36Elongation factor Tu OS=Clostridium acetobutylicum... [more]
EFTU_DESDG9.657e-1135.35Elongation factor Tu OS=Desulfovibrio desulfurican... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-102-E02-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY701055 ID=EY701055; Name=EY701055; organism=Citrus sinensis; type=EST; length=957bp
TTGCGGCCGCGCCGGCCCCCCAACNTGTGTTCNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGAACCATTTTCGCGAATAT
CATTTTAATAAGGTTTTTTCTACATTTGCATAATGGGTAAGGAGAACACT
CACATCAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCAC
CACCGGACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCG
AGAGGTTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATAC
GCCTGGGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCAT
TGATATTGCCTTGTGGAAGTTCGAGACCACCAGGTACTACTGCACCGTCA
TTGATGCTCCTGGGACATCGTGACTTTATCAAGAACATGATTACCGGTAC
CTCACAGGCTGACTGTGCCGTCCTTATCATTGACTCTACCACCGGTGGTT
TTGAAGCTGGTATCTCCCGGGCAGGGTCAGACTCCTGGACCATGCTCGTT
GCTTGGCTTCCACCCTTGGGTGTGGAAGCCAGACTGAGGTGGGCTGGCGG
GTACGGACGGATGGATTGCCGGGCGACCCCCCAAGGTACGCCACAGGCGG
TACGCGTCCGACGCAAAATTTCGTGAAAGGGAGCGTCCTCCTTTCCGTGC
CCTTGAAGGAACCGGCGTTGGAGTGCCCCCCCCCCGCAGCACATTTCTCC
CCTTCCGTGGCCCCCACCTCCCGCGGTTTGCAGGGCGTTACCAGCCTGCA
GTCGCTTAAGTGGCCCACCCGGCGTTCGGCCGGGGGTTAGGGGGGGGGCG
TGGGGGGGGGGGGGGGGGCCGCGCACGCCGCGCCGCCCCGCCCCGGAGGG
GGCGGGCCCGCCCGGCCCGCCGCGCGGGCTGGCCGGCCCCCCCCCCCAGC
GCCGGCC
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