DC900381 | Citrus Genome Database

Overview


Name	DC900381
Unique Name	DC900381
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	656

Libraries


Library Name	Type
VSS	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig10814	contig	Csv1_Contig10814:273..927.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2G2_BOVIN (Ubiquitin-conjugating enzyme E2 G2 OS=Bos taurus GN=UBE2G2 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 1.798e-22
Identity = 48/78 (61.54%), Postives = 61/78 (78.21%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRS 267
            +GRVCISILH PGDDP GYE ++ERW PV +VE I+LS++SML+ PNDES ANV+A+K WRD R+ F K     V++S
Sbjct:   85 DGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKVAKQIVQKS 162

HSP 2 Score: 22.7126 bits (47), Expect = 1.798e-22
Identity = 6/10 (60.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:    6 EIWHPNVYPE 35
            E++HPN+YP+
Sbjct:   76 EMFHPNIYPD 85

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2G2_PONAB (Ubiquitin-conjugating enzyme E2 G2 OS=Pongo abelii GN=UBE2G2 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.340e-22
Identity = 48/78 (61.54%), Postives = 61/78 (78.21%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRS 267
            +GRVCISILH PGDDP GYE ++ERW PV +VE I+LS++SML+ PNDES ANV+A+K WRD R+ F K     V++S
Sbjct:   85 DGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKS 162

HSP 2 Score: 22.7126 bits (47), Expect = 2.340e-22
Identity = 6/10 (60.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:    6 EIWHPNVYPE 35
            E++HPN+YP+
Sbjct:   76 EMFHPNIYPD 85

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2G2_MOUSE (Ubiquitin-conjugating enzyme E2 G2 OS=Mus musculus GN=Ube2g2 PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.340e-22
Identity = 48/78 (61.54%), Postives = 61/78 (78.21%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRS 267
            +GRVCISILH PGDDP GYE ++ERW PV +VE I+LS++SML+ PNDES ANV+A+K WRD R+ F K     V++S
Sbjct:   85 DGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKS 162

HSP 2 Score: 22.7126 bits (47), Expect = 2.340e-22
Identity = 6/10 (60.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:    6 EIWHPNVYPE 35
            E++HPN+YP+
Sbjct:   76 EMFHPNIYPD 85

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2G2_HUMAN (Ubiquitin-conjugating enzyme E2 G2 OS=Homo sapiens GN=UBE2G2 PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.340e-22
Identity = 48/78 (61.54%), Postives = 61/78 (78.21%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRS 267
            +GRVCISILH PGDDP GYE ++ERW PV +VE I+LS++SML+ PNDES ANV+A+K WRD R+ F K     V++S
Sbjct:   85 DGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKS 162

HSP 2 Score: 22.7126 bits (47), Expect = 2.340e-22
Identity = 6/10 (60.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:    6 EIWHPNVYPE 35
            E++HPN+YP+
Sbjct:   76 EMFHPNIYPD 85

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UBC7_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC7 PE=1 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 1.477e-21
Identity = 47/78 (60.26%), Postives = 59/78 (75.64%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRS 267
            NG VCISILH PGDDPN YELA ERW PV +VE I+LS++SMLS PN ES AN++A   WRD R +F+++V   + +S
Sbjct:   85 NGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLSEPNIESGANIDACILWRDNRPEFERQVKLSILKS 162

HSP 2 Score: 22.7126 bits (47), Expect = 1.477e-21
Identity = 7/10 (70.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:    3 PEIWHPNVYP 32
            P I HPN+YP
Sbjct:   75 PSILHPNIYP 84

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UBC7_SCHPO (Ubiquitin-conjugating enzyme E2-18 kDa OS=Schizosaccharomyces pombe GN=ubc3 PE=2 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 2.324e-20
Identity = 44/79 (55.70%), Postives = 61/79 (77.22%), Query Frame = 1

Query:   31 RNGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRS 267
            ++G VCISILH PGDDPN YE +SERW PV +VE I+LS++SML+ PNDES AN++A K WR+ R+++ + V    R++
Sbjct:   85 KDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKILLSVMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLARKT 163

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2R2_RABIT (Ubiquitin-conjugating enzyme E2 R2 OS=Oryctolagus cuniculus GN=UBE2R2 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.106e-15
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAA---KEWRDRRDDFKK 240
            NG VCISILHPP DDP   EL SERW P   V +I+LS+IS+L+ PN  SPANV+A+   ++WRD +   K+
Sbjct:   89 NGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKE 160

HSP 2 Score: 21.557 bits (44), Expect = 2.106e-15
Identity = 5/8 (62.50%), Postives = 8/8 (100.00%), Query Frame = 3

Query:    6 EIWHPNVY 29
            ++WHPN+Y
Sbjct:   80 KMWHPNIY 87

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2R2_MOUSE (Ubiquitin-conjugating enzyme E2 R2 OS=Mus musculus GN=Ube2r2 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.106e-15
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAA---KEWRDRRDDFKK 240
            NG VCISILHPP DDP   EL SERW P   V +I+LS+IS+L+ PN  SPANV+A+   ++WRD +   K+
Sbjct:   89 NGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKE 160

HSP 2 Score: 21.557 bits (44), Expect = 2.106e-15
Identity = 5/8 (62.50%), Postives = 8/8 (100.00%), Query Frame = 3

Query:    6 EIWHPNVY 29
            ++WHPN+Y
Sbjct:   80 KMWHPNIY 87

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UB2R2_HUMAN (Ubiquitin-conjugating enzyme E2 R2 OS=Homo sapiens GN=UBE2R2 PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.106e-15
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = 1

Query:   34 NGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAA---KEWRDRRDDFKK 240
            NG VCISILHPP DDP   EL SERW P   V +I+LS+IS+L+ PN  SPANV+A+   ++WRD +   K+
Sbjct:   89 NGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKE 160

HSP 2 Score: 21.557 bits (44), Expect = 2.106e-15
Identity = 5/8 (62.50%), Postives = 8/8 (100.00%), Query Frame = 3

Query:    6 EIWHPNVY 29
            ++WHPN+Y
Sbjct:   80 KMWHPNIY 87

[Close]

BLAST of DC900381 vs. ExPASy Swiss-Prot
Match: UBC3_YEAST (Ubiquitin-conjugating enzyme E2-34 kDa OS=Saccharomyces cerevisiae GN=CDC34 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.007e-14
Identity = 40/81 (49.38%), Postives = 57/81 (70.37%), Query Frame = 1

Query:   31 RNGRVCISILHPPGDDPNGYELASERWMPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDRRDDFKKKVGXCVRRSQE 273
            R+GR+CISILH  GD P   E  +E W PV TVES+++SI+S+L  PN  SPANV+AA ++R   + +K++V   V RS++
Sbjct:   90 RDGRLCISILHQSGD-PMTDEPDAETWSPVQTVESVLISIVSLLEDPNINSPANVDAAVDYRKNPEQYKQRVKMEVERSKQ 169

HSP 2 Score: 21.557 bits (44), Expect = 1.007e-14
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 3

Query:    3 PEIWHPNVY 29
            P I+HPNVY
Sbjct:   81 PAIYHPNVY 89

The following BLAST results are available for this feature:

BLAST of DC900381 vs. ExPASy Swiss-Prot

BLAST of DC900381 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 22


Match Name	E-value	Identity	Description
UB2G2_BOVIN	1.798e-22	61.54	Ubiquitin-conjugating enzyme E2 G2 OS=Bos taurus G...	[more]
UB2G2_PONAB	2.340e-22	61.54	Ubiquitin-conjugating enzyme E2 G2 OS=Pongo abelii...	[more]
UB2G2_MOUSE	2.340e-22	61.54	Ubiquitin-conjugating enzyme E2 G2 OS=Mus musculus...	[more]
UB2G2_HUMAN	2.340e-22	61.54	Ubiquitin-conjugating enzyme E2 G2 OS=Homo sapiens...	[more]
UBC7_YEAST	1.477e-21	60.26	Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo...	[more]
UBC7_SCHPO	2.324e-20	55.70	Ubiquitin-conjugating enzyme E2-18 kDa OS=Schizosa...	[more]
UB2R2_RABIT	2.106e-15	55.56	Ubiquitin-conjugating enzyme E2 R2 OS=Oryctolagus ...	[more]
UB2R2_MOUSE	2.106e-15	55.56	Ubiquitin-conjugating enzyme E2 R2 OS=Mus musculus...	[more]
UB2R2_HUMAN	2.106e-15	55.56	Ubiquitin-conjugating enzyme E2 R2 OS=Homo sapiens...	[more]
UBC3_YEAST	1.007e-14	49.38	Ubiquitin-conjugating enzyme E2-34 kDa OS=Saccharo...	[more]

Pages

Properties


Property Name	Value
Genbank description	DC900381 VSS Citrus sinensis cDNA clone VS28953 3, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DC900381 ID=DC900381; Name=DC900381; organism=Citrus sinensis; type=EST; length=656bp
CACCAGAGATTTGGCATCCTAATGTCTATCCGGAATGGGCGTGTCTGCAT
ATCAATCCTTCATCCTCCAGGTGACGACCCTAATGGATATGAGCTTGCAA
GTGAGCGCTGGATGCCTGTTCATACTGTTGAAAGTATCGTTTTGAGTATC
ATATCAATGCTTTCCGGTCCTAATGATGAATCTCCTGCAAATGTCGAAGC
TGCAAAAGAATGGAGAGACAGGAGAGATGACTTCAAGAAGAAAGTAGGCC
NCTGTGTGAGAAGATCNCAAGAAATGTTGTAATCCAACTGCAGGCACTCT
TTTTTTCTCTACCTATGTGTTTGTACGGGAAATGTCTTGATTTAATTGTG
TGGGGGGAACTGGGTTCTGTGGGTAATGTCAATGTGCTTGGTCTTGTGTA
GACTGGCATTTTGAAGTTCTCTCAACCATCTCTCTCGTTTTCTCTGCATG
GTCAAGTCNCTGTAACGACTGGGAACATCCATGTATTCAAAAGCGNNNTT
TGGTTAGTTTTAATCNCAAATGTTCTGTCATTCTCTTAAGTTCGTGTGGA
GATTGCTCTACTCAGAATGGCTTGTGGTTTGATTTCAAAAGGATTCCCCC
GTGATATTTGCTGCTTTTGAATCTTACCGAGCCCNCAAGCATTAGATGTT
TTCTTT