DC900339

Overview
NameDC900339
Unique NameDC900339
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length689
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC16_ARATH (Probable ubiquitin carrier protein E2 16 OS=Arabidopsis thaliana GN=UBC16 PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 6.563e-14
Identity = 22/37 (59.46%), Postives = 30/37 (81.08%), Query Frame = 1 
Query:  277 HPNINSNGSICLDILKEQWSPALTISKVLLSICSLLT 387
            HP+I SNG ICLDIL + WSPA+T+S + +SI S+L+
Sbjct:   89 HPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLS 125

HSP 2 Score: 45.0542 bits (105), Expect = 6.563e-14
Identity = 21/71 (29.58%), Postives = 38/71 (53.52%), Query Frame = 3 
Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S++A+ R+ KEL + Q +PP       V D++  W   ++G   + +A   + + + FP  YP + P+V F
Sbjct:   13 SKIATNRLQKELVEWQMNPPTGFKH-KVTDNLQRWIIEVIGAPGTLYANDTYQLQVDFPEHYPMESPQVIF 82
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UB12L_ARATH (Probable NEDD8-conjugating enzyme Ubc12-like OS=Arabidopsis thaliana GN=RCE2 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 8.458e-14
Identity = 29/69 (42.03%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  265 TKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTAR 471
            TKV+HPNI+  G++CL+IL+E W P L I+ V+  +  L T+PN +DPL  E A + + +   +E   R
Sbjct:  100 TKVYHPNIDLEGNVCLNILREDWKPVLNINTVIYGLFHLFTEPNYEDPLNHEAAAVLRDNPKTFEYNVR 168

HSP 2 Score: 31.5722 bits (70), Expect = 8.458e-14
Identity = 19/82 (23.17%), Postives = 36/82 (43.90%), Query Frame = 3 
Query:    9 QTQTLRPQSLLRNSQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKV 254
            Q+   R  S ++       R++K++ +L        S     +D+ +++ TI  P +  +  G F+ S      YP + PKV
Sbjct:   16 QSNNGRGASTVKKQSAGELRLHKDISELNLPKSCKISFPNGKNDLMNFEVTIK-PDEGYYLSGNFVFSFQVSNMYPHEAPKV 96
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC8_SCHPO (Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe GN=ubc8 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.099e-13
Identity = 27/79 (34.18%), Postives = 43/79 (54.43%), Query Frame = 1 
Query:  262 VTKVFHPNINS-NGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            V ++FHPNI+  +GS+CLD++ + WSP   +  +  + +  LL  PN  DPL  E A +   +   Y    R +  +YA
Sbjct:   69 VNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNASDPLNGEAAALLLREPNTYYAKVRDYIARYA 147

HSP 2 Score: 43.8986 bits (102), Expect = 1.099e-13
Identity = 16/44 (36.36%), Postives = 28/44 (63.64%), Query Frame = 3 
Query:  129 VADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            V D+M  +     GPS++P++GG++ V +  P +YP+K P + F
Sbjct:   25 VNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGF 68
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC18_ARATH (Probable ubiquitin carrier protein E2 18 OS=Arabidopsis thaliana GN=UBC18 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.870e-13
Identity = 23/37 (62.16%), Postives = 30/37 (81.08%), Query Frame = 1 
Query:  277 HPNINSNGSICLDILKEQWSPALTISKVLLSICSLLT 387
            HP+I SNG ICLDIL + WSPA+T+S V +SI S+L+
Sbjct:   89 HPHIYSNGHICLDILYDSWSPAMTVSSVCISILSMLS 125

HSP 2 Score: 43.1282 bits (100), Expect = 1.870e-13
Identity = 21/71 (29.58%), Postives = 36/71 (50.70%), Query Frame = 3 
Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S++A  R+ KEL + Q +PP       V D++  W   + G   + +A   + + + FP  YP + P+V F
Sbjct:   13 SKIACNRLQKELSEWQVNPPTGFKHR-VTDNLQKWVIEVTGAPGTLYANETYNLQVEFPQHYPMEAPQVIF 82
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC15_ARATH (Ubiquitin-conjugating enzyme 15 OS=Arabidopsis thaliana GN=UBC15 PE=2 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 1.870e-13
Identity = 22/37 (59.46%), Postives = 30/37 (81.08%), Query Frame = 1 
Query:  277 HPNINSNGSICLDILKEQWSPALTISKVLLSICSLLT 387
            HP+I SNG ICLDIL + WSPA+T++ V +SI S+L+
Sbjct:   89 HPHIYSNGHICLDILYDSWSPAMTVNSVCISILSMLS 125

HSP 2 Score: 44.2838 bits (103), Expect = 1.870e-13
Identity = 21/71 (29.58%), Postives = 36/71 (50.70%), Query Frame = 3 
Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S++A  R+ KEL + Q +PP       V D++  W   + G   + +A   + + + FP  YP + P+V F
Sbjct:   13 SKIACNRLQKELSEWQLNPPTGFRH-KVTDNLQKWTIDVTGAPGTLYANETYQLQVEFPEHYPMEAPQVVF 82
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UB2L6_MOUSE (Ubiquitin/ISG15-conjugating enzyme E2 L6 OS=Mus musculus GN=Ube2l6 PE=2 SV=3)

HSP 1 Score: 48.9062 bits (115), Expect = 1.876e-13
Identity = 22/78 (28.21%), Postives = 44/78 (56.41%), Query Frame = 1 
Query:  265 TKVFHPNINSNGSICLDIL-KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYAM 495
            TK++HPN+  +G +CL ++  E W P     +VL ++  L++ PN ++P+  E+A +   +   +   A  +T K+ +
Sbjct:   72 TKIYHPNVREDGLVCLPLISNENWKPYTKPYQVLEALNVLVSKPNLEEPVRLELADLLTQNPEMFRKKAEEFTLKFGV 149

HSP 2 Score: 47.7506 bits (112), Expect = 1.876e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 3 
Query:   54 MASKRINKELKDLQKD-PPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            MASKR+ KEL+ L K+ PP          ++  W   ++ P   P+    F V I FP +YPFKPP + F
Sbjct:    2 MASKRVAKELESLSKELPPYLRQLSSDDANVLVWHMLLL-PDQLPYGLKAFQVRIDFPREYPFKPPTLRF 70
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2H_MOUSE (Ubiquitin-conjugating enzyme E2 H OS=Mus musculus GN=Ube2h PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.130e-13
Identity = 28/79 (35.44%), Postives = 47/79 (59.49%), Query Frame = 1 
Query:  262 VTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            + K+FHPNI+ ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   71 MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 38.1206 bits (87), Expect = 3.130e-13
Identity = 14/31 (45.16%), Postives = 19/31 (61.29%), Query Frame = 3 
Query:  168 GPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            GP  +P+ GGV+ V +  P  YPFK P + F
Sbjct:   40 GPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF 70
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2H_HUMAN (Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens GN=UBE2H PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.130e-13
Identity = 28/79 (35.44%), Postives = 47/79 (59.49%), Query Frame = 1 
Query:  262 VTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            + K+FHPNI+ ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   71 MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 38.1206 bits (87), Expect = 3.130e-13
Identity = 14/31 (45.16%), Postives = 19/31 (61.29%), Query Frame = 3 
Query:  168 GPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            GP  +P+ GGV+ V +  P  YPFK P + F
Sbjct:   40 GPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF 70
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2H_BOVIN (Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN=UBE2H PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.130e-13
Identity = 28/79 (35.44%), Postives = 47/79 (59.49%), Query Frame = 1 
Query:  262 VTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            + K+FHPNI+ ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   71 MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 38.1206 bits (87), Expect = 3.130e-13
Identity = 14/31 (45.16%), Postives = 19/31 (61.29%), Query Frame = 3 
Query:  168 GPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            GP  +P+ GGV+ V +  P  YPFK P + F
Sbjct:   40 GPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF 70
[Close]
BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2W_DICDI (Probable ubiquitin-conjugating enzyme E2 W OS=Dictyostelium discoideum GN=ube2w PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.127e-13
Identity = 25/41 (60.98%), Postives = 30/41 (73.17%), Query Frame = 1 
Query:  265 TKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLT 387
            T   HP+I SNG ICL IL + WSPALT+S V LSI S+L+
Sbjct:   74 TPPIHPHIYSNGHICLSILYDNWSPALTVSSVCLSILSMLS 114

HSP 2 Score: 41.9726 bits (97), Expect = 4.127e-13
Identity = 25/74 (33.78%), Postives = 36/74 (48.65%), Query Frame = 3 
Query:   60 SKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRY*GFPP 281
            +KR+ KEL DL+ +PP  C +    D++  W   + G   S + G  F +   F   YP   P+V F   G PP
Sbjct:    6 AKRLQKELLDLKTNPPP-CISITEGDNLDKWVIAVDGTEGSIYQGEHFKLQFKFSSGYPLDSPEVIFI--GTPP 76
The following BLAST results are available for this feature:
BLAST of DC900339 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 230
Match NameE-valueIdentityDescription
UBC16_ARATH6.563e-1459.46Probable ubiquitin carrier protein E2 16 OS=Arabid... [more]
UB12L_ARATH8.458e-1442.03Probable NEDD8-conjugating enzyme Ubc12-like OS=Ar... [more]
UBC8_SCHPO1.099e-1334.18Ubiquitin-conjugating enzyme E2 8 OS=Schizosacchar... [more]
UBC18_ARATH1.870e-1362.16Probable ubiquitin carrier protein E2 18 OS=Arabid... [more]
UBC15_ARATH1.870e-1359.46Ubiquitin-conjugating enzyme 15 OS=Arabidopsis tha... [more]
UB2L6_MOUSE1.876e-1328.21Ubiquitin/ISG15-conjugating enzyme E2 L6 OS=Mus mu... [more]
UBE2H_MOUSE3.130e-1335.44Ubiquitin-conjugating enzyme E2 H OS=Mus musculus ... [more]
UBE2H_HUMAN3.130e-1335.44Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens ... [more]
UBE2H_BOVIN3.130e-1335.44Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN... [more]
UBE2W_DICDI4.127e-1360.98Probable ubiquitin-conjugating enzyme E2 W OS=Dict... [more]

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Properties
Property NameValue
Genbank descriptionDC900339 VSS Citrus sinensis cDNA clone VS28690 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900339 ID=DC900339; Name=DC900339; organism=Citrus sinensis; type=EST; length=689bp
AATAACACCAGACCCAAACTCTCAGGCCACAAAGTTTGTTGAGAAATTCC
CAGATGGCTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGA
CCCTCCTGCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATT
GGCAAGCAACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTA
TTTCTTGTATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAA
GGTGGCCTTCCGTTACTAAGGTTTTCCACCCTAACATCAACAGCAATGGT
AGCATCTGTCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAAT
ATCCAAGGTACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAG
ATGACCCACTTGTGCCTGAGATTGCTCACATGTACAAGACTGACAGAGCC
AAATATGAGACTACTGCTCGATCCTGGACCCAGAAGTATGCCATGGGTTA
AGAGGGGCTAGGCTGTACGCCCCTGAGAACATTAATGGCTTGTGGTCCTA
TTAGTAAATAATAGAAATAAGTTTGTTTCAGATATTAAAAGACGATATTT
GGTTTTGTTTACTAAGTTGTTAAAAGGTTTTATGTGGGGTTACTTTCCAA
TTTAGCATTGTCCTTCAATACTTAAATTATAATTTACTG
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