DC900339 | Citrus Genome Database

Overview


Name	DC900339
Unique Name	DC900339
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	689

Libraries


Library Name	Type
VSS	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.391e-26
Identity = 31/66 (46.97%), Postives = 49/66 (74.24%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYE 459
            ++K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ ++ +YE
Sbjct:   73 LSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 65.4698 bits (158), Expect = 1.391e-26
Identity = 29/71 (40.85%), Postives = 40/71 (56.34%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K LQ+DPP   S  P  +++  W A I GP  +PF  G F + I F  +YP KPP V F
Sbjct:    2 STPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.391e-26
Identity = 31/66 (46.97%), Postives = 49/66 (74.24%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYE 459
            ++K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ ++ +YE
Sbjct:   73 LSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 65.4698 bits (158), Expect = 1.391e-26
Identity = 29/71 (40.85%), Postives = 40/71 (56.34%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K LQ+DPP   S  P  +++  W A I GP  +PF  G F + I F  +YP KPP V F
Sbjct:    2 STPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.391e-26
Identity = 31/66 (46.97%), Postives = 49/66 (74.24%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYE 459
            ++K+FHPN+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ ++ +YE
Sbjct:   73 LSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 65.4698 bits (158), Expect = 1.391e-26
Identity = 29/71 (40.85%), Postives = 40/71 (56.34%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K LQ+DPP   S  P  +++  W A I GP  +PF  G F + I F  +YP KPP V F
Sbjct:    2 STPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2T_XENLA (Ubiquitin-conjugating enzyme E2 T OS=Xenopus laevis GN=ube2t PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.793e-26
Identity = 41/82 (50.00%), Postives = 59/82 (71.95%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYAM 495
            +T ++HPNI+S G ICLDILK      W PAL IS VL SI  L+++PNPDDPL+ +I+  +K +RA + + A+ WT+K+A+
Sbjct:   71 LTPIYHPNIDSAGRICLDILKLPPKGAWRPALNISTVLTSIQLLMSEPNPDDPLMADISSEFKYNRAVFFSNAKKWTEKHAL 152

HSP 2 Score: 54.299 bits (129), Expect = 1.793e-26
Identity = 26/65 (40.00%), Postives = 38/65 (58.46%), Query Frame = 3

Query:   66 RINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            R+ +EL+ L K+PP          +M   +A I+G S SP+ GG+F + I  P  YPF+PPK+ F
Sbjct:    6 RLKRELQLLNKEPPPGVICWQNESNMDDLRAQIIGGSGSPYEGGIFNLEIIVPERYPFEPPKIRF 70

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_USTMA (Ubiquitin-conjugating enzyme E2 2 OS=Ustilago maydis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.338e-26
Identity = 33/77 (42.86%), Postives = 49/77 (63.64%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKY-----ETTARSW 477
            ++K+FHPN+ +NG +CLDIL+ +WSP   ++ +L SI SLL DPNP+ P   E A +Y+ +  +Y      T   SW
Sbjct:   73 LSKMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLHDPNPNSPANAEAASLYRENMKEYVRRVKATVEASW 149

HSP 2 Score: 67.0106 bits (162), Expect = 2.338e-26
Identity = 30/71 (42.25%), Postives = 40/71 (56.34%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  + KR+ ++ K L  DPP   S  P AD++  W A I GP+D+PF  G F + + F   YP KPP V F
Sbjct:    2 STPSKKRLIRDFKRLSTDPPGGISGAPCADNLMIWNAVIFGPADTPFEDGTFKLVLTFDESYPNKPPTVKF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_DICDI (Ubiquitin-conjugating enzyme E2 2 OS=Dictyostelium discoideum GN=ubc2 PE=3 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 2.357e-26
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKY-----ETTARSWT 480
            ++K+FHPN+ ++GSICLDIL+ QWSP   I+ +L SI SLL DPNP+ P   E A +++ ++ +Y     E   +SW+
Sbjct:   73 LSKMFHPNVYADGSICLDILQNQWSPIYDIAAILTSIQSLLCDPNPNSPANSESARLFRENKREYNRKVKEIVEQSWS 150

HSP 2 Score: 63.929 bits (154), Expect = 2.357e-26
Identity = 29/71 (40.85%), Postives = 41/71 (57.75%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K LQ DPPA  S  P+ +++  WQA I GP DS + G  F +S+ F  +YP   P V F
Sbjct:    2 STAARRRLMRDFKRLQSDPPAGISGAPLENNILMWQAVIFGPDDSIWEGATFKLSLQFSEEYPNDAPTVKF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_CANGA (Ubiquitin-conjugating enzyme E2 2 OS=Candida glabrata GN=UBC2 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.176e-26
Identity = 32/77 (41.56%), Postives = 50/77 (64.94%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKY-----ETTARSW 477
            ++++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K  +++Y     ET  +SW
Sbjct:   73 LSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYIKRVKETVEKSW 149

HSP 2 Score: 65.855 bits (159), Expect = 5.176e-26
Identity = 28/71 (39.44%), Postives = 44/71 (61.97%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  DYP KPP V F
Sbjct:    2 STPARRRLMRDFKRMKEDSPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEDYPNKPPHVKF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_YEAST (Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces cerevisiae GN=RAD6 PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.484e-25
Identity = 32/77 (41.56%), Postives = 50/77 (64.94%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKY-----ETTARSW 477
            ++++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K  +++Y     ET  +SW
Sbjct:   73 LSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSW 149

HSP 2 Score: 64.3142 bits (155), Expect = 1.484e-25
Identity = 27/71 (38.03%), Postives = 44/71 (61.97%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  +YP KPP V F
Sbjct:    2 STPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_ASHGO (Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossypii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.485e-25
Identity = 32/77 (41.56%), Postives = 50/77 (64.94%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKY-----ETTARSW 477
            ++++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K  +++Y     ET  +SW
Sbjct:   73 LSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSW 149

HSP 2 Score: 64.3142 bits (155), Expect = 1.485e-25
Identity = 27/71 (38.03%), Postives = 44/71 (61.97%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  +YP KPP V F
Sbjct:    2 STPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC27_ARATH (Ubiquitin carrier protein E2 27 OS=Arabidopsis thaliana GN=UBC27 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.504e-25
Identity = 40/77 (51.95%), Postives = 55/77 (71.43%), Query Frame = 1

Query:  265 TKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            TKV+HPNI+S +G+ICLDILK+QWSPALT+   L+SI +LL+ P P DP    +A  Y  +   + +TAR WT+ +A
Sbjct:   73 TKVWHPNISSQSGAICLDILKDQWSPALTLKTALVSIQALLSAPEPKDPQDAVVAEQYMKNYQVFVSTARYWTETFA 149

HSP 2 Score: 54.299 bits (129), Expect = 2.504e-25
Identity = 26/66 (39.39%), Postives = 39/66 (59.09%), Query Frame = 3

Query:   66 RINKELKDLQKDPPAS-CSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            RI KEL+D +++  +S     P +D++     TI GP  +P+ GG F + I  P  YPF+PPK+ F
Sbjct:    6 RIQKELQDCERNQDSSGIRVCPKSDNLTRLTGTIPGPIGTPYEGGTFQIDITMPDGYPFEPPKMQF 71

The following BLAST results are available for this feature:

BLAST of DC900339 vs. ExPASy Swiss-Prot

BLAST of DC900339 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 230


Match Name	E-value	Identity	Description
UBE2B_MOUSE	1.391e-26	46.97	Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ...	[more]
UBE2B_HUMAN	1.391e-26	46.97	Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ...	[more]
UBE2B_BOVIN	1.391e-26	46.97	Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN...	[more]
UBE2T_XENLA	1.793e-26	50.00	Ubiquitin-conjugating enzyme E2 T OS=Xenopus laevi...	[more]
UBC2_USTMA	2.338e-26	42.86	Ubiquitin-conjugating enzyme E2 2 OS=Ustilago mayd...	[more]
UBC2_DICDI	2.357e-26	44.87	Ubiquitin-conjugating enzyme E2 2 OS=Dictyostelium...	[more]
UBC2_CANGA	5.176e-26	41.56	Ubiquitin-conjugating enzyme E2 2 OS=Candida glabr...	[more]
UBC2_YEAST	1.484e-25	41.56	Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces...	[more]
UBC2_ASHGO	1.485e-25	41.56	Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossyp...	[more]
UBC27_ARATH	2.504e-25	51.95	Ubiquitin carrier protein E2 27 OS=Arabidopsis tha...	[more]

Pages

Properties


Property Name	Value
Genbank description	DC900339 VSS Citrus sinensis cDNA clone VS28690 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DC900339 ID=DC900339; Name=DC900339; organism=Citrus sinensis; type=EST; length=689bp
AATAACACCAGACCCAAACTCTCAGGCCACAAAGTTTGTTGAGAAATTCC
CAGATGGCTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGA
CCCTCCTGCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATT
GGCAAGCAACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTA
TTTCTTGTATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAA
GGTGGCCTTCCGTTACTAAGGTTTTCCACCCTAACATCAACAGCAATGGT
AGCATCTGTCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAAT
ATCCAAGGTACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAG
ATGACCCACTTGTGCCTGAGATTGCTCACATGTACAAGACTGACAGAGCC
AAATATGAGACTACTGCTCGATCCTGGACCCAGAAGTATGCCATGGGTTA
AGAGGGGCTAGGCTGTACGCCCCTGAGAACATTAATGGCTTGTGGTCCTA
TTAGTAAATAATAGAAATAAGTTTGTTTCAGATATTAAAAGACGATATTT
GGTTTTGTTTACTAAGTTGTTAAAAGGTTTTATGTGGGGTTACTTTCCAA
TTTAGCATTGTCCTTCAATACTTAAATTATAATTTACTG