DC900339 | Citrus Genome Database

Overview


Name	DC900339
Unique Name	DC900339
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	689

Libraries


Library Name	Type
VSS	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_KLULA (Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces lactis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.268e-25
Identity = 31/77 (40.26%), Postives = 50/77 (64.94%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKY-----ETTARSW 477
            ++++FHPN+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A +++  +++Y     ET  +SW
Sbjct:   73 LSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFQDHKSQYVKRVKETVEKSW 149

HSP 2 Score: 64.3142 bits (155), Expect = 4.268e-25
Identity = 27/71 (38.03%), Postives = 44/71 (61.97%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K +++D P   SA P+ D++  W A I+GP+D+P+  G F + + F  +YP KPP V F
Sbjct:    2 STPARRRLMRDFKRMKEDSPPGVSASPLPDNVMIWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBC2_CRYNE (Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus neoformans GN=UBC2 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.734e-24
Identity = 33/77 (42.86%), Postives = 49/77 (63.64%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYE-----TTARSW 477
            ++K+FHPNI +NG +CLDIL+ +WSP   ++ +L S+ SLL DPNP  P   + A ++K +  +YE     T   SW
Sbjct:   73 ISKMFHPNIYANGELCLDILQNRWSPTYDVAAILTSVQSLLNDPNPASPANVDAAQLFKENLKEYERRVKKTVELSW 149

HSP 2 Score: 58.151 bits (139), Expect = 7.734e-24
Identity = 27/71 (38.03%), Postives = 38/71 (53.52%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            S  A +R+ ++ K L  D P   S  P  D++  W A I GP ++PF  G F +++ F   YP KPP V F
Sbjct:    2 STAAKRRLIRDFKRLTSDAPIGISGSPNPDNIMVWNAVIFGPPETPFEDGSFRLTLTFTDAYPNKPPTVRF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2T_BOVIN (Ubiquitin-conjugating enzyme E2 T OS=Bos taurus GN=UBE2T PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.303e-23
Identity = 35/81 (43.21%), Postives = 54/81 (66.67%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            +T ++HPNI+S G ICLD+LK      W P+L I+ +L  I  L+ +PNPDDPL+ +I+  +K ++  +   AR WT+K+A
Sbjct:   71 LTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATLLTCIQQLMAEPNPDDPLMADISSEFKYNKPVFFKNARQWTEKHA 151

HSP 2 Score: 53.5286 bits (127), Expect = 1.303e-23
Identity = 23/67 (34.33%), Postives = 39/67 (58.21%), Query Frame = 3

Query:   60 SKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            + R+ +EL  L  +PP   +     D M   +A I+G +++P+  GVF + +H P  YPF+PP++ F
Sbjct:    4 TSRLKRELSLLAAEPPPGITCWQDGDRMEDLRAQILGGANTPYEKGVFKLEVHIPERYPFEPPQIRF 70

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UE2NL_HUMAN (Putative ubiquitin-conjugating enzyme E2 N-like OS=Homo sapiens GN=UBE2NL PE=5 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.316e-23
Identity = 42/78 (53.85%), Postives = 57/78 (73.08%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYAM 495
            +TK++HPN++    I LDILK++WSPAL I  VLLSI +LL  PNPDDPL  ++   +KT+ A+   TAR+WT+ YAM
Sbjct:   73 MTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVEQWKTNEAQAIETARAWTRLYAM 150

HSP 2 Score: 40.0466 bits (92), Expect = 1.316e-23
Identity = 24/66 (36.36%), Postives = 31/66 (46.97%), Query Frame = 3

Query:   66 RINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPS-DSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            RI KE + L  +P     A P   +  ++   I G S DSPF GG F   +    +YP   PKV F
Sbjct:    7 RIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRF 72

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2T_HUMAN (Ubiquitin-conjugating enzyme E2 T OS=Homo sapiens GN=UBE2T PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.206e-23
Identity = 37/81 (45.68%), Postives = 56/81 (69.14%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            +T ++HPNI+S G ICLD+LK      W P+L I+ VL SI  L+++PNPDDPL+ +I+  +K ++  +   AR WT+K+A
Sbjct:   71 LTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHA 151

HSP 2 Score: 49.6766 bits (117), Expect = 2.206e-23
Identity = 22/67 (32.84%), Postives = 38/67 (56.72%), Query Frame = 3

Query:   60 SKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            + R+ +EL  L  +PP   +     D M   +A I+G +++P+  GVF + +  P  YPF+PP++ F
Sbjct:    4 ASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRF 70

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2C_DICDI (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum GN=ube2c PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.773e-23
Identity = 32/77 (41.56%), Postives = 48/77 (62.34%), Query Frame = 1

Query:  265 TKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT--------DRAKYETTAR 471
            T+ FHPN+++NG+ICLDILK++WSP   +  +L+SI SLL +PN + PL    A ++          D+   E T+R
Sbjct:   76 TQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNESPLNSYAASLWSNQDEYKKVLDKRYQEATSR 152

HSP 2 Score: 62.7734 bits (151), Expect = 3.773e-23
Identity = 31/74 (41.89%), Postives = 41/74 (55.41%), Query Frame = 3

Query:   60 SKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRY*GFPP 281
            SKR+  EL +L   P    SA P  D++F+W  TI G  D+ +    F +S+ FP DYP+KPP V F    F P
Sbjct:    8 SKRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKFPTDYPYKPPTVKFETQCFHP 81

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2K_MOUSE (Ubiquitin-conjugating enzyme E2 K OS=Mus musculus GN=Ube2k PE=2 SV=3)

HSP 1 Score: 80.4925 bits (197), Expect = 4.859e-23
Identity = 38/78 (48.72%), Postives = 54/78 (69.23%), Query Frame = 1

Query:  262 VTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            +TK++HPNI+S  G+ICLDILK+QW+ A+T+  VLLS+ +LL    PDDP    +A+ YK +   ++ TAR W   YA
Sbjct:   76 ITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 48.521 bits (114), Expect = 4.859e-23
Identity = 25/74 (33.78%), Postives = 38/74 (51.35%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPAS---CSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            + +A +RI +E K++ K    S        V ++    +  I GP D+P+ GG + + I  P  YPF PPKV F
Sbjct:    2 ANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRF 75

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2K_HUMAN (Ubiquitin-conjugating enzyme E2 K OS=Homo sapiens GN=UBE2K PE=1 SV=3)

HSP 1 Score: 80.4925 bits (197), Expect = 4.859e-23
Identity = 38/78 (48.72%), Postives = 54/78 (69.23%), Query Frame = 1

Query:  262 VTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            +TK++HPNI+S  G+ICLDILK+QW+ A+T+  VLLS+ +LL    PDDP    +A+ YK +   ++ TAR W   YA
Sbjct:   76 ITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 48.521 bits (114), Expect = 4.859e-23
Identity = 25/74 (33.78%), Postives = 38/74 (51.35%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPAS---CSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            + +A +RI +E K++ K    S        V ++    +  I GP D+P+ GG + + I  P  YPF PPKV F
Sbjct:    2 ANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRF 75

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2K_BOVIN (Ubiquitin-conjugating enzyme E2 K OS=Bos taurus GN=UBE2K PE=1 SV=3)

HSP 1 Score: 80.4925 bits (197), Expect = 4.859e-23
Identity = 38/78 (48.72%), Postives = 54/78 (69.23%), Query Frame = 1

Query:  262 VTKVFHPNINS-NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            +TK++HPNI+S  G+ICLDILK+QW+ A+T+  VLLS+ +LL    PDDP    +A+ YK +   ++ TAR W   YA
Sbjct:   76 ITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYA 153

HSP 2 Score: 48.521 bits (114), Expect = 4.859e-23
Identity = 25/74 (33.78%), Postives = 38/74 (51.35%), Query Frame = 3

Query:   48 SQMASKRINKELKDLQKDPPAS---CSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            + +A +RI +E K++ K    S        V ++    +  I GP D+P+ GG + + I  P  YPF PPKV F
Sbjct:    2 ANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRF 75

[Close]

BLAST of DC900339 vs. ExPASy Swiss-Prot
Match: UBE2T_MOUSE (Ubiquitin-conjugating enzyme E2 T OS=Mus musculus GN=Ube2t PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.069e-22
Identity = 36/81 (44.44%), Postives = 55/81 (67.90%), Query Frame = 1

Query:  262 VTKVFHPNINSNGSICLDILK----EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYA 492
            +T ++HPNI+S+G ICLDILK      W P+L I+ VL SI  L+ +PNPDDPL+ +I+  +K ++  +   A+ WT+ +A
Sbjct:   71 LTPIYHPNIDSSGRICLDILKLPPKGAWRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFLKKAKQWTEAHA 151

HSP 2 Score: 50.0618 bits (118), Expect = 1.069e-22
Identity = 23/67 (34.33%), Postives = 38/67 (56.72%), Query Frame = 3

Query:   60 SKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAF 260
            + R+ KEL  L  +PP   +     D +   +A I+G +++P+  GVF + +  P  YPF+PP+V F
Sbjct:    4 ASRLKKELHMLAIEPPPGITCWQEKDQVADLRAQILGGANTPYEKGVFTLEVIIPERYPFEPPQVRF 70

The following BLAST results are available for this feature:

BLAST of DC900339 vs. ExPASy Swiss-Prot

BLAST of DC900339 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 230


Match Name	E-value	Identity	Description
UBC2_KLULA	4.268e-25	40.26	Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces...	[more]
UBC2_CRYNE	7.734e-24	42.86	Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus ...	[more]
UBE2T_BOVIN	1.303e-23	43.21	Ubiquitin-conjugating enzyme E2 T OS=Bos taurus GN...	[more]
UE2NL_HUMAN	1.316e-23	53.85	Putative ubiquitin-conjugating enzyme E2 N-like OS...	[more]
UBE2T_HUMAN	2.206e-23	45.68	Ubiquitin-conjugating enzyme E2 T OS=Homo sapiens ...	[more]
UBE2C_DICDI	3.773e-23	41.56	Probable ubiquitin-conjugating enzyme E2 C OS=Dict...	[more]
UBE2K_MOUSE	4.859e-23	48.72	Ubiquitin-conjugating enzyme E2 K OS=Mus musculus ...	[more]
UBE2K_HUMAN	4.859e-23	48.72	Ubiquitin-conjugating enzyme E2 K OS=Homo sapiens ...	[more]
UBE2K_BOVIN	4.859e-23	48.72	Ubiquitin-conjugating enzyme E2 K OS=Bos taurus GN...	[more]
UBE2T_MOUSE	1.069e-22	44.44	Ubiquitin-conjugating enzyme E2 T OS=Mus musculus ...	[more]

Pages

Properties


Property Name	Value
Genbank description	DC900339 VSS Citrus sinensis cDNA clone VS28690 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DC900339 ID=DC900339; Name=DC900339; organism=Citrus sinensis; type=EST; length=689bp
AATAACACCAGACCCAAACTCTCAGGCCACAAAGTTTGTTGAGAAATTCC
CAGATGGCTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGA
CCCTCCTGCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATT
GGCAAGCAACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTA
TTTCTTGTATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAA
GGTGGCCTTCCGTTACTAAGGTTTTCCACCCTAACATCAACAGCAATGGT
AGCATCTGTCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAAT
ATCCAAGGTACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAG
ATGACCCACTTGTGCCTGAGATTGCTCACATGTACAAGACTGACAGAGCC
AAATATGAGACTACTGCTCGATCCTGGACCCAGAAGTATGCCATGGGTTA
AGAGGGGCTAGGCTGTACGCCCCTGAGAACATTAATGGCTTGTGGTCCTA
TTAGTAAATAATAGAAATAAGTTTGTTTCAGATATTAAAAGACGATATTT
GGTTTTGTTTACTAAGTTGTTAAAAGGTTTTATGTGGGGTTACTTTCCAA
TTTAGCATTGTCCTTCAATACTTAAATTATAATTTACTG