DC900307

Overview
NameDC900307
Unique NameDC900307
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length635
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:162..796. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK2_PICSI (S-adenosylmethionine synthetase 2 OS=Picea sitchensis GN=METK2 PE=2 SV=1)

HSP 1 Score: 345.895 bits (886), Expect = 1.019e-94
Identity = 164/183 (89.62%), Postives = 172/183 (93.99%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA +DYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVT EY N+ GAM
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLIQDPDSKVACETCTKTNMVMVFGEITTKADIDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYRNEGGAM 183
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK2_DAUCA (S-adenosylmethionine synthetase 2 OS=Daucus carota GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 345.895 bits (886), Expect = 1.019e-94
Identity = 166/183 (90.71%), Postives = 171/183 (93.44%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            M+TFL TSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETC+KTNMVMVFGEITTKA VDYEKIVR TCR IGFVSDDVGLDAD+CKVLV IEQQSPDIAQGVHGH TKRPEDIGAGDQGHMFGYATDET E MPLSHVLATKLGA+LTEVRKN TC WLRPDGKTQVT EYYND GAM
Sbjct:    1 MDTFLNTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCSKTNMVMVFGEITTKANVDYEKIVRKTCRDIGFVSDDVGLDADNCKVLVQIEQQSPDIAQGVHGHLTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYYNDKGAM 183
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK2_HORVU (S-adenosylmethionine synthetase 2 OS=Hordeum vulgare GN=SAM2 PE=2 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 1.331e-94
Identity = 163/182 (89.56%), Postives = 172/182 (94.51%), Query Frame = 3 
Query:   90 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE++GAGDQG MFGYATDET E MPL+H+LATKLGARLTEVRKN TC+WLRPDGKTQVT EY N+ GAM
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRDIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEVGAGDQGIMFGYATDETPELMPLTHMLATKLGARLTEVRKNGTCAWLRPDGKTQVTIEYLNEGGAM 185
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 1.331e-94
Identity = 163/182 (89.56%), Postives = 174/182 (95.60%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGA 632
            ++TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVR+TCR+IGFVS DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN TC+WLRPDGKTQVT EY N++GA
Sbjct:    4 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYRNESGA 185
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 345.51 bits (885), Expect = 1.331e-94
Identity = 163/182 (89.56%), Postives = 174/182 (95.60%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGA 632
            ++TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVR+TCR+IGFVS DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN TC+WLRPDGKTQVT EY N++GA
Sbjct:    4 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYRNESGA 185
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 1.331e-94
Identity = 163/182 (89.56%), Postives = 172/182 (94.51%), Query Frame = 3 
Query:   90 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE++GAGDQG MFGYATDET E MPL+H+LATKLGARLTEVRKN TC+WLRPDGKTQVT EY N+ GAM
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRDIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEVGAGDQGIMFGYATDETPELMPLTHMLATKLGARLTEVRKNGTCAWLRPDGKTQVTIEYLNEGGAM 185
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK1_TOBAC (S-adenosylmethionine synthetase 1 OS=Nicotiana tabacum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 345.125 bits (884), Expect = 1.739e-94
Identity = 164/183 (89.62%), Postives = 171/183 (93.44%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HV ATKLGA+LTEVRKN TC WLRPDGKTQVT EY NDNGAM
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVWATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDNGAM 183
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK_PINBN (S-adenosylmethionine synthetase OS=Pinus banksiana GN=METK PE=2 SV=1)

HSP 1 Score: 344.739 bits (883), Expect = 2.271e-94
Identity = 165/183 (90.16%), Postives = 170/183 (92.90%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MP  HVLATKLGA+LTEVRKN TC WLRP GKTQVT EY NDNGAM
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETLELMPKCHVLATKLGAKLTEVRKNGTCPWLRPHGKTQVTVEYRNDNGAM 183
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK2_ACTCH (S-adenosylmethionine synthetase 2 OS=Actinidia chinensis GN=SAM2 PE=2 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 2.966e-94
Identity = 162/183 (88.52%), Postives = 173/183 (94.54%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            ME+FLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH +K+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN+TC WLRPDGKTQVT EY N+ GAM
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNNTCPWLRPDGKTQVTVEYRNEGGAM 183
[Close]
BLAST of DC900307 vs. ExPASy Swiss-Prot
Match: METK1_VITVI (S-adenosylmethionine synthetase 1 OS=Vitis vinifera GN=METK1 PE=3 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 2.966e-94
Identity = 163/183 (89.07%), Postives = 173/183 (94.54%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTXEYYNDNGAM 635
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN TC WLRPDGKTQVT EY N+ GAM
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTVEYRNEGGAM 183
The following BLAST results are available for this feature:
BLAST of DC900307 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK2_PICSI1.019e-9489.62S-adenosylmethionine synthetase 2 OS=Picea sitchen... [more]
METK2_DAUCA1.019e-9490.71S-adenosylmethionine synthetase 2 OS=Daucus carota... [more]
METK2_HORVU1.331e-9489.56S-adenosylmethionine synthetase 2 OS=Hordeum vulga... [more]
METK1_ORYSJ1.331e-9489.56S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK1_ORYSI1.331e-9489.56S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK1_HORVU1.331e-9489.56S-adenosylmethionine synthetase 1 OS=Hordeum vulga... [more]
METK1_TOBAC1.739e-9489.62S-adenosylmethionine synthetase 1 OS=Nicotiana tab... [more]
METK_PINBN2.271e-9490.16S-adenosylmethionine synthetase OS=Pinus banksiana... [more]
METK2_ACTCH2.966e-9488.52S-adenosylmethionine synthetase 2 OS=Actinidia chi... [more]
METK1_VITVI2.966e-9489.07S-adenosylmethionine synthetase 1 OS=Vitis vinifer... [more]

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Properties
Property NameValue
Genbank descriptionDC900307 VSS Citrus sinensis cDNA clone VS28659 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900307 ID=DC900307; Name=DC900307; organism=Citrus sinensis; type=EST; length=635bp
TTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCTTAGAT
CTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACCTTTCT
ATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTGCGACC
AGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTGAGAGC
AAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTCTTTGG
AGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCGCGACA
CTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACGCCGAT
CACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATTGCCCA
GGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGCTGGTG
ACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAATTTATG
CCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACGGAGGT
TAGGAAGAATAGTACCTGCTCTTGGCTAAGGCCCGATGGGAAGACCCAGG
TCACTGNTGAGTACTACAACGACAATGGTGCCATG
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