CN186084

Overview
NameCN186084
Unique NameCN186084
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length718
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: CD48A_ARATH (Cell division control protein 48 homolog A OS=Arabidopsis thaliana GN=CDC48A PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.947e-18
Identity = 38/67 (56.72%), Postives = 58/67 (86.57%), Query Frame = 1
Query:  517 EKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            E++ D  YD +GG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+GKTL+ARAVA+ T
Sbjct:  199 ERLDDVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANET 265          

HSP 2 Score: 77.411 bits (189), Expect = 8.467e-14
Identity = 33/66 (50.00%), Postives = 51/66 (77.27%), Query Frame = 1
Query:  514 VEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V +VP+ +++ IGGL+   +E++E ++ P++HPE FE  G++  KGVL YGPPG GKTLLA+A+A+
Sbjct:  471 VVEVPNVSWNDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKTLLAKAIAN 536          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6A_SCHPO (26S protease regulatory subunit 6A OS=Schizosaccharomyces pombe GN=tbp1 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.322e-18
Identity = 45/95 (47.37%), Postives = 62/95 (65.26%), Query Frame = 1
Query:  424 PSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            P   + +  DSY++   LPS+ D  V  M+V++ P   Y  IGGL +QI+E+ E I LP++  + F  LG+  PKG L++GPPGTGKTLLARA A
Sbjct:  146 PGDLIGVNKDSYLIIDKLPSEYDSRVKAMEVDEKPTERYSDIGGLSKQIEELFEAIVLPMQQADKFRKLGVKPPKGCLMFGPPGTGKTLLARACA 240          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: TERA2_CAEEL (Transitional endoplasmic reticulum ATPase homolog 2 OS=Caenorhabditis elegans GN=cdc-48.2 PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 5.666e-18
Identity = 38/76 (50.00%), Postives = 61/76 (80.26%), Query Frame = 1
Query:  490 DPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            DP+    + E + +  YD +GG+ +Q+ +IKE++ELP++HP+LF+++G+  P+G+LL+GPPGTGKTL+ARAVA+ T
Sbjct:  192 DPIKREEEEEALNEVGYDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLIARAVANET 267          

HSP 2 Score: 71.633 bits (174), Expect = 4.646e-12
Identity = 30/66 (45.45%), Postives = 48/66 (72.73%), Query Frame = 1
Query:  514 VEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V + P++T+  IGGL    +E++E+++ P++HPE +   G+   +GVL YGPPG GKTLLA+A+A+
Sbjct:  473 VVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIAN 538          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: CDC48_SOYBN (Cell division cycle protein 48 homolog OS=Glycine max GN=CDC48 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.666e-18
Identity = 37/67 (55.22%), Postives = 58/67 (86.57%), Query Frame = 1
Query:  517 EKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            E++ +  YD +GG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+GKTL+ARAVA+ T
Sbjct:  200 ERLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANET 266          

HSP 2 Score: 77.7962 bits (190), Expect = 6.483e-14
Identity = 33/66 (50.00%), Postives = 51/66 (77.27%), Query Frame = 1
Query:  514 VEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V +VP+ +++ IGGL+   +E++E ++ P++HPE FE  G++  KGVL YGPPG GKTLLA+A+A+
Sbjct:  472 VVEVPNVSWEDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKTLLAKAIAN 537          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: CDC48_CAPAN (Cell division cycle protein 48 homolog OS=Capsicum annuum GN=CAFP PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.666e-18
Identity = 37/67 (55.22%), Postives = 58/67 (86.57%), Query Frame = 1
Query:  517 EKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            E++ +  YD +GG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+GKTL+ARAVA+ T
Sbjct:  200 ERLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANET 266          

HSP 2 Score: 73.9442 bits (180), Expect = 9.361e-13
Identity = 32/66 (48.48%), Postives = 50/66 (75.76%), Query Frame = 1
Query:  514 VEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V +VP+ +++ IGGL+   +E++E ++ P++ PE FE  G++  KGVL YGPPG GKTLLA+A+A+
Sbjct:  472 VVEVPNVSWEDIGGLENVKRELQETVQYPVEPPEKFEKFGMSPSKGVLFYGPPGCGKTLLAKAIAN 537          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: CD48E_ARATH (Cell division control protein 48 homolog E OS=Arabidopsis thaliana GN=CDC48E PE=2 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 5.666e-18
Identity = 37/67 (55.22%), Postives = 58/67 (86.57%), Query Frame = 1
Query:  517 EKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            E++ +  YD +GG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+GKTL+ARAVA+ T
Sbjct:  199 ERLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANET 265          

HSP 2 Score: 77.7962 bits (190), Expect = 6.483e-14
Identity = 33/66 (50.00%), Postives = 51/66 (77.27%), Query Frame = 1
Query:  514 VEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V +VP+ +++ IGGL+   +E++E ++ P++HPE FE  G++  KGVL YGPPG GKTLLA+A+A+
Sbjct:  471 VVEVPNVSWEDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKTLLAKAIAN 536          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: CD48D_ARATH (Cell division control protein 48 homolog D OS=Arabidopsis thaliana GN=CDC48D PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.666e-18
Identity = 37/67 (55.22%), Postives = 58/67 (86.57%), Query Frame = 1
Query:  517 EKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            E++ +  YD +GG+ +Q+ +I+E++ELP++HP+LF+S+G+  PKG+LLYGPPG+GKTL+ARAVA+ T
Sbjct:  200 ERLDEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANET 266          

HSP 2 Score: 77.7962 bits (190), Expect = 6.483e-14
Identity = 33/66 (50.00%), Postives = 51/66 (77.27%), Query Frame = 1
Query:  514 VEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V +VP+ +++ IGGL+   +E++E ++ P++HPE FE  G++  KGVL YGPPG GKTLLA+A+A+
Sbjct:  472 VVEVPNVSWEDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKTLLAKAIAN 537          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: TERA_DANRE (Transitional endoplasmic reticulum ATPase OS=Danio rerio GN=vcp PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.400e-18
Identity = 39/76 (51.32%), Postives = 60/76 (78.95%), Query Frame = 1
Query:  490 DPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            +P+    + E + +  YD IGG+ +Q+ +IKE++ELP++HP LF+++G+  P+G+LLYGPPGTGKTL+ARAVA+ T
Sbjct:  187 EPIKREDEEESLNEVGYDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANET 262          

HSP 2 Score: 77.0258 bits (188), Expect = 1.106e-13
Identity = 35/77 (45.45%), Postives = 54/77 (70.13%), Query Frame = 1
Query:  481 SKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            S+ +P      V +VP+ T++ IGGLD   +E++E+++ P++HP+ F   G+   KGVL YGPPG GKTLLA+A+A+
Sbjct:  457 SQSNPSALRETVVEVPNITWEDIGGLDDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIAN 533          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: CDC48_EMENI (Cell division control protein 48 OS=Emericella nidulans GN=cdc48 PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.400e-18
Identity = 40/82 (48.78%), Postives = 63/82 (76.83%), Query Frame = 1
Query:  472 ILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            I+ S+ +P+    +   + +  YD IGG  +Q+ +I+E++ELP++HP+LF+S+GI  P+G+L+YGPPGTGKTL+ARAVA+ T
Sbjct:  206 IIHSEGEPIQREDEENNLNEVGYDDIGGCRKQMAQIRELVELPLRHPQLFKSIGIKPPRGILMYGPPGTGKTLMARAVANET 287          

HSP 2 Score: 77.7962 bits (190), Expect = 6.483e-14
Identity = 39/95 (41.05%), Postives = 61/95 (64.21%), Query Frame = 1
Query:  427 STRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            S  V + N  Y L +  PS +  +     V +VP+  ++ IGGL++  +E+ E ++ P+ HPE F+  G++  +GVL YGPPGTGKT+LA+AVA+
Sbjct:  469 SLGVTMENFRYALGVSNPSALREVA----VVEVPNVRWEDIGGLEEVKRELIESVQYPVDHPEKFQKFGLSPSRGVLFYGPPGTGKTMLAKAVAN 559          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: TERA_XENTR (Transitional endoplasmic reticulum ATPase OS=Xenopus tropicalis GN=vcp PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.262e-17
Identity = 39/76 (51.32%), Postives = 59/76 (77.63%), Query Frame = 1
Query:  490 DPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            +P+    + E + +  YD IGG  +Q+ +IKE++ELP++HP LF+++G+  P+G+LLYGPPGTGKTL+ARAVA+ T
Sbjct:  187 EPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANET 262          

HSP 2 Score: 75.0998 bits (183), Expect = 4.202e-13
Identity = 34/77 (44.16%), Postives = 53/77 (68.83%), Query Frame = 1
Query:  481 SKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            S+ +P      V +VP  T++ IGGL+   +E++E+++ P++HP+ F   G+   KGVL YGPPG GKTLLA+A+A+
Sbjct:  457 SQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIAN 533          
The following BLAST results are available for this feature:
BLAST of CN186084 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 183
Match NameE-valueIdentityDescription
CD48A_ARATH1.947e-1856.72Cell division control protein 48 homolog A OS=Arab... [more]
PRS6A_SCHPO3.322e-1847.3726S protease regulatory subunit 6A OS=Schizosaccha... [more]
TERA2_CAEEL5.666e-1850.00Transitional endoplasmic reticulum ATPase homolog ... [more]
CDC48_SOYBN5.666e-1855.22Cell division cycle protein 48 homolog OS=Glycine ... [more]
CDC48_CAPAN5.666e-1855.22Cell division cycle protein 48 homolog OS=Capsicum... [more]
CD48E_ARATH5.666e-1855.22Cell division control protein 48 homolog E OS=Arab... [more]
CD48D_ARATH5.666e-1855.22Cell division control protein 48 homolog D OS=Arab... [more]
TERA_DANRE7.400e-1851.32Transitional endoplasmic reticulum ATPase OS=Danio... [more]
CDC48_EMENI7.400e-1848.78Cell division control protein 48 OS=Emericella nid... [more]
TERA_XENTR1.262e-1751.32Transitional endoplasmic reticulum ATPase OS=Xenop... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002F11_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002F11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186084 ID=CN186084; Name=CN186084; organism=Citrus sinensis; type=EST; length=718bp
GGAGACTTGATCCCAAAGACAATTAGATTCGAACAAATCGAACTCGTTCA
CTCGGAAAGAATGGCCGCTGTAGGAATCGAAACGAAGCAGCCGGAATCGG
CGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGCATCGAAACAAGGAGAG
GGATTGAGGCAATACTACCTCCAACACATTCACGAACTCCAGCTCCAAGT
CCGTCAAAAAACCCACAATCTCAATCGCCTCGAAGCCCAGCGTAACGAAC
TCAATTCTAGAGTGCGGATGCTTAAAGAAGAATTACAGCTTCTTCAAGAG
CCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTATGGGAAAAAATAAGGT
TCTTGTTAAGGTTCATCCTGAGGGGAAATATGTTGTTGATATTGATAAAA
GTATTGATATAACCAAGATCACTCCGTCAACAAGAGTTGCACTTCGTAAT
GACAGCTATGTTCTTCATTTGATCCTGCCAAGCAAAGTAGATCCCCTGGT
CAACCTTATGAAGGTTGAAAAGGTCCCTGATTCTACATATGACATGATTG
GTGGCCTTGATCAGCAAATTAAGGAGATCAAGGAGGTCATTGAGCTTCCT
ATCAAACATCCCGAATTGTTCGAGTCTCTTGGAATAGCTCAACCCAAGGG
TGTATTGCTATATGGACCACCTGGTACTGGCAAAACACTGTTGGCTAGAG
CTGTGGCTCATCATACTG
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