CN186084

Overview
NameCN186084
Unique NameCN186084
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length718
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PEX6_PICPA (Peroxisomal biogenesis factor 6 OS=Pichia pastoris GN=PEX6 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.085e-12
Identity = 33/63 (52.38%), Postives = 48/63 (76.19%), Query Frame = 1
Query:  520 KVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            ++P+  ++ +GGLD    EI + I++P+KHPELF + GI +  G+L YGPPGTGKTLLA+A+A
Sbjct:  812 RIPNVKWEDVGGLDVVKDEILDTIDMPMKHPELFSN-GIKKRSGILFYGPPGTGKTLLAKAIA 873          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: TBP7_CAEBR (TAT-binding homolog 7 OS=Caenorhabditis briggsae GN=lex-1 PE=3 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 3.557e-12
Identity = 31/58 (53.45%), Postives = 43/58 (74.14%), Query Frame = 1
Query:  538 YDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            +D +GGL   I+ +KEV+  P+ +PE+F    I  PKGV+ YGPPGTGKTL+ARA+A+
Sbjct:  404 FDQVGGLSHHIQSLKEVVLFPMLYPEVFAKFKINPPKGVVFYGPPGTGKTLVARALAN 461          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: RIX7_YEAST (Ribosome biogenesis ATPase RIX7 OS=Saccharomyces cerevisiae GN=RIX7 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.557e-12
Identity = 35/65 (53.85%), Postives = 46/65 (70.77%), Query Frame = 1
Query:  523 VPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            VPD T+  +G L +   E+   I  PIK PEL+E +GI+ P GVLL+GPPG GKTLLA+AVA+ +
Sbjct:  527 VPDVTWANVGALQRVRLELNMAIVQPIKRPELYEKVGISAPGGVLLWGPPGCGKTLLAKAVANES 591          

HSP 2 Score: 71.633 bits (174), Expect = 4.646e-12
Identity = 31/61 (50.82%), Postives = 45/61 (73.77%), Query Frame = 1
Query:  526 PDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            P+S+   +GG+D  + ++ E+I LPI HPE+F S G+  P+GVLL+GPPG GKT +A A+A
Sbjct:  200 PNSSLKSLGGMDDVVAQLMELIGLPILHPEIFLSTGVEPPRGVLLHGPPGCGKTSIANALA 260          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: ATD2B_HUMAN (ATPase family AAA domain-containing protein 2B OS=Homo sapiens GN=ATAD2B PE=1 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 3.557e-12
Identity = 34/71 (47.89%), Postives = 48/71 (67.61%), Query Frame = 1
Query:  499 VNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            V+ M ++K     +D IGGL   I  +KE++  P+ +PE+FE   I  P+G L YGPPGTGKTL+ARA+A+
Sbjct:  388 VDPMNIDK--SVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALAN 456          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: NVL_DICDI (Putative ribosome biogenesis ATPase nvl OS=Dictyostelium discoideum GN=nvl PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 6.068e-12
Identity = 29/62 (46.77%), Postives = 46/62 (74.19%), Query Frame = 1
Query:  523 VPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            +P   +  +GG++  +++I+E IE PI HPE++  LG+  P+G+LL+GP G GKTLLA+A+A
Sbjct:  210 IPTINFSNLGGVESCLRDIREHIEYPICHPEIYSHLGVEPPRGILLHGPSGCGKTLLAKAIA 271          

HSP 2 Score: 67.3958 bits (163), Expect = 8.761e-11
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 1
Query:  484 KVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            KV P         +P+ T+D +G L    +E+   I  PI++P+ ++++GI  P GVL+YGPPG GKTLLA+A+A
Sbjct:  547 KVVPAAKREGFATIPNVTWDDVGALSGVREELTNSILRPIRYPKKYKNMGIDSPAGVLMYGPPGCGKTLLAKAIA 621          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: ATAD2_PONAB (ATPase family AAA domain-containing protein 2 OS=Pongo abelii GN=ATAD2 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.924e-12
Identity = 30/58 (51.72%), Postives = 42/58 (72.41%), Query Frame = 1
Query:  538 YDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            +D +GGL   I  +KE++  P+ +PE+FE   I  P+G L YGPPGTGKTL+ARA+A+
Sbjct:  256 FDSVGGLSNHIAALKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALAN 313          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: ATAD2_MOUSE (ATPase family AAA domain-containing protein 2 OS=Mus musculus GN=Atad2 PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.924e-12
Identity = 30/58 (51.72%), Postives = 42/58 (72.41%), Query Frame = 1
Query:  538 YDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            +D +GGL   I  +KE++  P+ +PE+FE   I  P+G L YGPPGTGKTL+ARA+A+
Sbjct:   80 FDSVGGLSSHIAALKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALAN 137          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: ATAD2_HUMAN (ATPase family AAA domain-containing protein 2 OS=Homo sapiens GN=ATAD2 PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.924e-12
Identity = 30/58 (51.72%), Postives = 42/58 (72.41%), Query Frame = 1
Query:  538 YDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 711
            +D +GGL   I  +KE++  P+ +PE+FE   I  P+G L YGPPGTGKTL+ARA+A+
Sbjct:  425 FDSVGGLSNHIAALKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALAN 482          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PEX6_HUMAN (Peroxisome assembly factor 2 OS=Homo sapiens GN=PEX6 PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 1.035e-11
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 1
Query:  520 KVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            K+P  ++  +GGL +  KEI E I+LP++HPEL  SLG+ +  G+LL+GPPGTGKTLLA+AVA
Sbjct:  698 KIPSVSWHDVGGLQEVKKEILETIQLPLEHPELL-SLGLRR-SGLLLHGPPGTGKTLLAKAVA 758          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PEX1_MOUSE (Peroxisome biogenesis factor 1 OS=Mus musculus GN=Pex1 PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 1.035e-11
Identity = 35/70 (50.00%), Postives = 47/70 (67.14%), Query Frame = 1
Query:  508 MKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            + + K  D  +D IGGL +  + + + I+LP K+PELF +L I Q  G+LLYGPPGTGKTLLA  VA  +
Sbjct:  830 VNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVVARES 899          
The following BLAST results are available for this feature:
BLAST of CN186084 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 183
Match NameE-valueIdentityDescription
PEX6_PICPA2.085e-1252.38Peroxisomal biogenesis factor 6 OS=Pichia pastoris... [more]
TBP7_CAEBR3.557e-1253.45TAT-binding homolog 7 OS=Caenorhabditis briggsae G... [more]
RIX7_YEAST3.557e-1253.85Ribosome biogenesis ATPase RIX7 OS=Saccharomyces c... [more]
ATD2B_HUMAN3.557e-1247.89ATPase family AAA domain-containing protein 2B OS=... [more]
NVL_DICDI6.068e-1246.77Putative ribosome biogenesis ATPase nvl OS=Dictyos... [more]
ATAD2_PONAB7.924e-1251.72ATPase family AAA domain-containing protein 2 OS=P... [more]
ATAD2_MOUSE7.924e-1251.72ATPase family AAA domain-containing protein 2 OS=M... [more]
ATAD2_HUMAN7.924e-1251.72ATPase family AAA domain-containing protein 2 OS=H... [more]
PEX6_HUMAN1.035e-1157.14Peroxisome assembly factor 2 OS=Homo sapiens GN=PE... [more]
PEX1_MOUSE1.035e-1150.00Peroxisome biogenesis factor 1 OS=Mus musculus GN=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002F11_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002F11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186084 ID=CN186084; Name=CN186084; organism=Citrus sinensis; type=EST; length=718bp
GGAGACTTGATCCCAAAGACAATTAGATTCGAACAAATCGAACTCGTTCA
CTCGGAAAGAATGGCCGCTGTAGGAATCGAAACGAAGCAGCCGGAATCGG
CGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGCATCGAAACAAGGAGAG
GGATTGAGGCAATACTACCTCCAACACATTCACGAACTCCAGCTCCAAGT
CCGTCAAAAAACCCACAATCTCAATCGCCTCGAAGCCCAGCGTAACGAAC
TCAATTCTAGAGTGCGGATGCTTAAAGAAGAATTACAGCTTCTTCAAGAG
CCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTATGGGAAAAAATAAGGT
TCTTGTTAAGGTTCATCCTGAGGGGAAATATGTTGTTGATATTGATAAAA
GTATTGATATAACCAAGATCACTCCGTCAACAAGAGTTGCACTTCGTAAT
GACAGCTATGTTCTTCATTTGATCCTGCCAAGCAAAGTAGATCCCCTGGT
CAACCTTATGAAGGTTGAAAAGGTCCCTGATTCTACATATGACATGATTG
GTGGCCTTGATCAGCAAATTAAGGAGATCAAGGAGGTCATTGAGCTTCCT
ATCAAACATCCCGAATTGTTCGAGTCTCTTGGAATAGCTCAACCCAAGGG
TGTATTGCTATATGGACCACCTGGTACTGGCAAAACACTGTTGGCTAGAG
CTGTGGCTCATCATACTG
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