CN186084

Overview
NameCN186084
Unique NameCN186084
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length718
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6B_MANSE (26S protease regulatory subunit 6B OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 3.314e-26
Identity = 65/185 (35.14%), Postives = 99/185 (53.51%), Query Frame = 1
Query:  184 ELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            +L  + ++    L  LE Q   +    R LK       EE++ +Q     +G+ ++ + +N  +V       Y V            PS  VAL   S  L  +LP + D  +++++ ++ PD  Y  IGG+D Q +EI+E +ELP+ H EL+  +GI  P+GVL+YGPPG GKT+LA AVAHHT
Sbjct:   36 DLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVELYRQIGIEPPRGVLMYGPPGCGKTMLANAVAHHT 220          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PSMR_SULTO (Proteasome-activating nucleotidase OS=Sulfolobus tokodaii GN=pan PE=3 SV=2)

HSP 1 Score: 117.472 bits (293), Expect = 7.383e-26
Identity = 70/185 (37.84%), Postives = 98/185 (52.97%), Query Frame = 1
Query:  154 LRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVA 708
            L  Y  +H +     VR     +  L  +  +L   +   K EL+ L  P      V+ ++   KV+V+       +V            P + VAL      +  +LP + D  V   +V + P+  Y  IGGL++QI EI+EVIELP+K+PELF+ +GI  PKGVLLYGPPGTGKTLLA+AVA
Sbjct:    5 LDSYRAKHYNSDDPIVRLLEEKIESLTKELEKLRQDLNWYKGELEKLLAPPYIEAIVLDILPDGKVIVRSSSGPNLIVNVSSNIDIKKLKPGSLVALTQRGSTIVEVLPEREDAYVKSFEVIEKPNVHYSDIGGLNEQINEIREVIELPLKNPELFKEIGIDPPKGVLLYGPPGTGKTLLAKAVA 189          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS7_DICDI (26S protease regulatory subunit 7 OS=Dictyostelium discoideum GN=psmC2 PE=1 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 7.383e-26
Identity = 69/193 (35.75%), Postives = 107/193 (55.44%), Query Frame = 1
Query:  166 YLQHIHELQLQVRQKTHNLNRLEAQRNELN-----SRVRMLKEELQLLQEPGSYVGEVVKVM--GKN--KVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            Y + I  L+  +++ T  +N L   +         S+  ++ ++    +EP   V    K++  GK   K ++ V    K+VV               RV +  + Y + + LP K+D  V +M+VE+ PD TY  +GG  +QI++++EV+E+P+ HPE F +LGI  PKGVL+YGPPGTGKTL ARAVA+ T
Sbjct:   36 YSKSIRILEEDIKKMTSKVNELCGIKESDTGIGPPSQWDLVVDKTSAHEEPPLQVARCTKIIDVGKPNAKYIITVKQIAKFVVALGDKLSPTDVEEGIRVGVDRNKYQIQIPLPPKIDASVTMMQVEEKPDITYKDVGGCKEQIEKLREVVEMPLLHPEKFVNLGIDPPKGVLMYGPPGTGKTLCARAVANRT 228          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6B_ARATH (26S protease regulatory subunit 6B homolog OS=Arabidopsis thaliana GN=RPT3 PE=1 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.259e-25
Identity = 60/160 (37.50%), Postives = 94/160 (58.75%), Query Frame = 1
Query:  238 QRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            ++  L   +   +EE++ +Q     +G+ ++++ +N  +V       Y V            PS  VAL   S  L  +LP + D  ++L+   + PD +Y+ IGG D Q +EI+E +ELP+ H EL++ +GI  P+GVLLYGPPGTGKT+LA+AVA+HT
Sbjct:   54 EQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVSYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANHT 213          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PSMR1_HALSA (Proteasome-activating nucleotidase 1 OS=Halobacterium salinarium GN=pan1 PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.645e-25
Identity = 61/166 (36.75%), Postives = 96/166 (57.83%), Query Frame = 1
Query:  220 LNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            L+ +EA+R EL   V  L+ E + L+    Y+  V  +      ++K H   + V+               RVA+ NDS+ +  +L  + D     M+V++ P  TY  IGGLD Q++E++E +E P+ +PE F+++G+  P GVLL+GPPGTGKT+LA+AVA+ T
Sbjct:   46 LDDVEARREELREEVNRLQRENETLKTASLYLATVEDLPEDGSAVIKQHGNNQEVLTELSPRLADTLEVGDRVAI-NDSFSVQRVLDDETDARAQAMEVDESPSVTYADIGGLDDQLREVREAVEDPLVNPEKFDAVGVEPPSGVLLHGPPGTGKTMLAKAVANQT 210          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS7_SCHPO (26S protease regulatory subunit 7 homolog OS=Schizosaccharomyces pombe GN=rpt1 PE=1 SV=2)

HSP 1 Score: 115.546 bits (288), Expect = 2.805e-25
Identity = 57/126 (45.24%), Postives = 77/126 (61.11%), Query Frame = 1
Query:  340 KNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            KN  ++ +    K+VV               RV    + Y + L LP K+DP V +M+VE+ PD TY  +GG  +QI+ ++EV+ELP+  PE F  LGI  PKG++LYGPPGTGKTL ARAVA+ T
Sbjct:  112 KNAYVINLKQIAKFVVSLGERVSPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTMMQVEEKPDVTYGDVGGCKEQIERLREVVELPLLSPERFVKLGIDPPKGIMLYGPPGTGKTLCARAVANRT 237          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6B_RAT (26S protease regulatory subunit 6B OS=Rattus norvegicus GN=Psmc4 PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 8.163e-25
Identity = 62/189 (32.80%), Postives = 101/189 (53.44%), Query Frame = 1
Query:  172 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H EL++ +GI  P+GVL+YGPPG GKT+LA+AVAHHT
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHT 223          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6B_MACFA (26S protease regulatory subunit 6B OS=Macaca fascicularis GN=PSMC4 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 8.163e-25
Identity = 62/189 (32.80%), Postives = 101/189 (53.44%), Query Frame = 1
Query:  172 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H EL++ +GI  P+GVL+YGPPG GKT+LA+AVAHHT
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHT 223          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6B_HUMAN (26S protease regulatory subunit 6B OS=Homo sapiens GN=PSMC4 PE=1 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 8.163e-25
Identity = 62/189 (32.80%), Postives = 101/189 (53.44%), Query Frame = 1
Query:  172 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H EL++ +GI  P+GVL+YGPPG GKT+LA+AVAHHT
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHT 223          
BLAST of CN186084 vs. ExPASy Swiss-Prot
Match: PRS6B_BOVIN (26S protease regulatory subunit 6B OS=Bos taurus GN=PSMC4 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 8.163e-25
Identity = 62/189 (32.80%), Postives = 101/189 (53.44%), Query Frame = 1
Query:  172 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHT 717
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H EL++ +GI  P+GVL+YGPPG GKT+LA+AVAHHT
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHT 223          
The following BLAST results are available for this feature:
BLAST of CN186084 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 183
Match NameE-valueIdentityDescription
PRS6B_MANSE3.314e-2635.1426S protease regulatory subunit 6B OS=Manduca sext... [more]
PSMR_SULTO7.383e-2637.84Proteasome-activating nucleotidase OS=Sulfolobus t... [more]
PRS7_DICDI7.383e-2635.7526S protease regulatory subunit 7 OS=Dictyostelium... [more]
PRS6B_ARATH1.259e-2537.5026S protease regulatory subunit 6B homolog OS=Arab... [more]
PSMR1_HALSA1.645e-2536.75Proteasome-activating nucleotidase 1 OS=Halobacter... [more]
PRS7_SCHPO2.805e-2545.2426S protease regulatory subunit 7 homolog OS=Schiz... [more]
PRS6B_RAT8.163e-2532.8026S protease regulatory subunit 6B OS=Rattus norve... [more]
PRS6B_MACFA8.163e-2532.8026S protease regulatory subunit 6B OS=Macaca fasci... [more]
PRS6B_HUMAN8.163e-2532.8026S protease regulatory subunit 6B OS=Homo sapiens... [more]
PRS6B_BOVIN8.163e-2532.8026S protease regulatory subunit 6B OS=Bos taurus G... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002F11_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002F11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186084 ID=CN186084; Name=CN186084; organism=Citrus sinensis; type=EST; length=718bp
GGAGACTTGATCCCAAAGACAATTAGATTCGAACAAATCGAACTCGTTCA
CTCGGAAAGAATGGCCGCTGTAGGAATCGAAACGAAGCAGCCGGAATCGG
CGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGCATCGAAACAAGGAGAG
GGATTGAGGCAATACTACCTCCAACACATTCACGAACTCCAGCTCCAAGT
CCGTCAAAAAACCCACAATCTCAATCGCCTCGAAGCCCAGCGTAACGAAC
TCAATTCTAGAGTGCGGATGCTTAAAGAAGAATTACAGCTTCTTCAAGAG
CCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTATGGGAAAAAATAAGGT
TCTTGTTAAGGTTCATCCTGAGGGGAAATATGTTGTTGATATTGATAAAA
GTATTGATATAACCAAGATCACTCCGTCAACAAGAGTTGCACTTCGTAAT
GACAGCTATGTTCTTCATTTGATCCTGCCAAGCAAAGTAGATCCCCTGGT
CAACCTTATGAAGGTTGAAAAGGTCCCTGATTCTACATATGACATGATTG
GTGGCCTTGATCAGCAAATTAAGGAGATCAAGGAGGTCATTGAGCTTCCT
ATCAAACATCCCGAATTGTTCGAGTCTCTTGGAATAGCTCAACCCAAGGG
TGTATTGCTATATGGACCACCTGGTACTGGCAAAACACTGTTGGCTAGAG
CTGTGGCTCATCATACTG
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