CN186026 | Citrus Genome Database

Overview


Name	CN186026
Unique Name	CN186026
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	775

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Pulp cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 2.961e-43
Identity = 60/100 (60.00%), Postives = 78/100 (78.00%), Query Frame = -1

Query:  467 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 766
            ++KRI +EL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F  DYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK
Sbjct:   62 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISK 161

HSP 2 Score: 60.4622 bits (145), Expect = 2.961e-43
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = -3

Query:  237 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 374
            KVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  161 KVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.961e-43
Identity = 60/100 (60.00%), Postives = 78/100 (78.00%), Query Frame = -1

Query:  467 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 766
            ++KRI +EL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F  DYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK
Sbjct:   62 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISK 161

HSP 2 Score: 60.4622 bits (145), Expect = 2.961e-43
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = -3

Query:  237 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 374
            KVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  161 KVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.961e-43
Identity = 60/100 (60.00%), Postives = 78/100 (78.00%), Query Frame = -1

Query:  467 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 766
            ++KRI +EL ++  DPP +CSAGP  ++++ W++TI+GP  S Y GGVF + I F  DYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK
Sbjct:   62 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISK 161

HSP 2 Score: 60.4622 bits (145), Expect = 2.961e-43
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = -3

Query:  237 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 374
            KVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  161 KVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: COP10_ARATH (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 2.333e-36
Identity = 53/100 (53.00%), Postives = 78/100 (78.00%), Query Frame = -1

Query:  467 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 766
            + KRI RE+ +L  DPP  CSAGP  ++++HW ATI+GPS +PY GG+F + I FP DYPFKPPK+ F+T+++H N+++ G + ++IL++ WSPALTI+K
Sbjct:   37 SGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITK 136

HSP 2 Score: 43.5134 bits (101), Expect = 2.333e-36
Identity = 18/46 (39.13%), Postives = 29/46 (63.04%), Query Frame = -3

Query:  237 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 374
            KVL +I S+   P P  P +P IA +Y +D+ K++  A+ WT ++A
Sbjct:  136 KVLQAIRSIFLKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFA 181

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC31_ARATH (Probable ubiquitin carrier protein E2 31 OS=Arabidopsis thaliana GN=UBC31 PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.181e-32
Identity = 42/80 (52.50%), Postives = 59/80 (73.75%), Query Frame = -1

Query:  467 SAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 706
            S G  + +++ W A I GP  +PY GG+F ++I FP DYPFKPPK  F+T ++HPNIN  GSIC++ILK++W+PAL + K
Sbjct:   28 SIGQNSNNIYEWTAVIRGPDGTPYEGGMFNLSIKFPTDYPFKPPKFTFKTPIYHPNINDEGSICMNILKDKWTPALMVEK 107

HSP 2 Score: 54.6842 bits (130), Expect = 3.181e-32
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = -3

Query:  237 KVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 374
            KVLLSI  LL  PNPDDPLVPEI  ++K+++ +++  AR +T ++A
Sbjct:  107 KVLLSILLLLEKPNPDDPLVPEIGQLFKNNRFQFDQRAREFTARHA 152

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBCB_DICDI (Ubiquitin conjugating enzyme E2 B OS=Dictyostelium discoideum GN=ubcB PE=1 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 9.167e-32
Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = -1

Query:  473 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNI-NSNGSICLDILKEQWSPALTI 766
            A KR+ +E+ D+ K PP+ CSA  V +++  W+AT+ GP  SP+  GVF + I  P DYPFKPP + F TK++HPNI  S+G+IC ++    WSP L I
Sbjct:    3 AHKRLQKEITDMLKTPPSWCSAHLVDDNLQKWKATVQGPEGSPFEKGVFSMDIDIPADYPFKPPTLKFTTKIYHPNIKTSDGAICAEVF-STWSPQLKI 100

HSP 2 Score: 58.151 bits (139), Expect = 9.167e-32
Identity = 25/45 (55.56%), Postives = 34/45 (75.56%), Query Frame = -3

Query:  237 VLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 371
            VL +I S+LTDPNPD+PL  EIA  +K+D+  +  TA+ WT+KYA
Sbjct:  103 VLTTIRSILTDPNPDNPLETEIAQQFKTDRNAFNKTAKEWTKKYA 147

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC36_ARATH (Ubiquitin carrier protein E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.021e-31
Identity = 52/109 (47.71%), Postives = 69/109 (63.30%), Query Frame = -1

Query:  434 KRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK*WRTTLWSCTA 760
            +RI +E + L  +P    SA P  E+M ++   I+GP+ SPY GGVF + +  P +YP   PKV F TK++HPNI+  G ICLDILK++WSPAL I    RT L S  A
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQI----RTVLLSIQA 112

HSP 2 Score: 53.1434 bits (126), Expect = 2.021e-31
Identity = 26/47 (55.32%), Postives = 34/47 (72.34%), Query Frame = -3

Query:  231 VLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG 371
            VLLSI +LL+ PNPDDPL   IA  +KS++A+   TA+ WT+ YA G
Sbjct:  106 VLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAKEWTRLYASG 152

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC35_ARATH (Ubiquitin carrier protein E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 4.464e-31
Identity = 51/109 (46.79%), Postives = 69/109 (63.30%), Query Frame = -1

Query:  434 KRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK*WRTTLWSCTA 760
            +RI +E + L  +P    SA P  ++M ++   I+GP+ SPY GGVF + +  P +YP   PKV F TK++HPNI+  G ICLDILK++WSPAL I    RT L S  A
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQI----RTVLLSIQA 112

HSP 2 Score: 53.1434 bits (126), Expect = 4.464e-31
Identity = 26/47 (55.32%), Postives = 34/47 (72.34%), Query Frame = -3

Query:  231 VLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG 371
            VLLSI +LL+ PNPDDPL   IA  +KS++A+   TA+ WT+ YA G
Sbjct:  106 VLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYASG 152

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBC3_ARATH (Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis thaliana GN=UBC3 PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.179e-30
Identity = 51/99 (51.52%), Postives = 67/99 (67.68%), Query Frame = -1

Query:  470 ASKRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTIS 766
            A KR+  + K LQKDPP   S  P   ++ HW A I GP D+P+ GG F +T+HF  DYP KPP V F +++FHPNI ++GSICLDIL+ QWSP   ++
Sbjct:    5 AKKRLMWDFKRLQKDPPVGISGAPQDNNIMHWNALIFGPEDTPWDGGTFKLTLHFTEDYPNKPPIVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVA 103

HSP 2 Score: 37.7354 bits (86), Expect = 2.179e-30
Identity = 18/39 (46.15%), Postives = 23/39 (58.97%), Query Frame = -3

Query:  273 IFGSCKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY 389
            I+    VL SI SLL DPNPD P   E A ++  +K +Y
Sbjct:   99 IYDVAAVLTSIQSLLCDPNPDSPANAEAARLFSENKREY 137

[Close]

BLAST of CN186026 vs. ExPASy Swiss-Prot
Match: UBE2N_DROME (Ubiquitin-conjugating enzyme E2 N OS=Drosophila melanogaster GN=ben PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.062e-29
Identity = 48/109 (44.04%), Postives = 68/109 (62.39%), Query Frame = -1

Query:  434 KRI*RELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK*WRTTLWSCTA 760
            +RI +E + L ++P    +A P   +  ++   + GP+DSP+ GGVF + +  P DYP   PKV F TK++HPNI+  G ICLD+LK++WSPAL I    RT L S  A
Sbjct:    6 RRIIKETQRLMQEPVPGINAIPDENNARYFHVIVTGPNDSPFEGGVFKLELFLPEDYPMSAPKVRFITKIYHPNIDRLGRICLDVLKDKWSPALQI----RTILLSIQA 110

HSP 2 Score: 54.299 bits (129), Expect = 1.062e-29
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = -3

Query:  234 VLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 371
            +LLSI +LL+ PNPDDPL  ++A ++K ++A+    AR WTQKYA+
Sbjct:  104 ILLSIQALLSAPNPDDPLANDVAELWKVNEAEAIRNAREWTQKYAV 149

The following BLAST results are available for this feature:

BLAST of CN186026 vs. ExPASy Swiss-Prot

BLAST of CN186026 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 245


Match Name	E-value	Identity	Description
UB2E3_MOUSE	2.961e-43	60.00	Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus...	[more]
UB2E3_HUMAN	2.961e-43	60.00	Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens...	[more]
UB2E3_BOVIN	2.961e-43	60.00	Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus G...	[more]
COP10_ARATH	2.333e-36	53.00	Constitutive photomorphogenesis protein 10 OS=Arab...	[more]
UBC31_ARATH	3.181e-32	52.50	Probable ubiquitin carrier protein E2 31 OS=Arabid...	[more]
UBCB_DICDI	9.167e-32	47.47	Ubiquitin conjugating enzyme E2 B OS=Dictyostelium...	[more]
UBC36_ARATH	2.021e-31	47.71	Ubiquitin carrier protein E2 36 OS=Arabidopsis tha...	[more]
UBC35_ARATH	4.464e-31	46.79	Ubiquitin carrier protein E2 35 OS=Arabidopsis tha...	[more]
UBC3_ARATH	2.179e-30	51.52	Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis t...	[more]
UBE2N_DROME	1.062e-29	44.04	Ubiquitin-conjugating enzyme E2 N OS=Drosophila me...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS05_0002D24_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002D24, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN186026 ID=CN186026; Name=CN186026; organism=Citrus sinensis; type=EST; length=775bp
TTTTTTTTTTTTTTTTTACTTAAGACCATTAACTACGTTCCATCGAAGAA
GCTTCAAACACAAACATTCAACATTAACATAAAAACGTCCCGCCCTTGCA
ACTCCTCCCAACACCATTCTAAAACACCAACGTCTGCCCTCCCCAAAGCA
AGTTAAAGAAAATAGATAGACAACACACACAACATATTTCAATAAATGCT
CCTTTTTCTGCAATATCCTAACAACCTTCATCCCATGGCATACTTCTGTG
TCCAGCTCCTGGCCGTGGATTCATATTTGGCCTTGTCGGACTTGTACATG
TGGGCAATCTCCGGTACAAGAGGGTCGTCGGGATTTGGATCAGTAAGCAA
GGAGCAGATAGAGAGCAGGACTTTGCAAGATCCAAAGATGCACTTGGGAG
TACAACTTTATGATCCCAGGGTCAGTTTTACCTGGCTGTGCAAGACCAGA
GAGTTGTACGCCACTACTTTGAAATGGTAAGCGCTGGACTCCACTGTTCT
TTAAGGATATCAAGACAGATGCTTCCATTGCTGTTGATGTTCGGGTGGAA
GACCTTGGTCCTAAAAGCAACCTTTGGGGGCTTGAAAGGATAATCAGGAG
GAAAATGAATGGTAACTAAAAATACACCTCCTGCATATGGGCTATCAGAA
GGGCCCATAATTGTTGCTTGCCAGTGGAACATGTCTTCAGCCACAGGGCC
AGCGCTGCAAGACGTGGGAGGATCCTTCTGCAAATCCTTGAGTTCCCTTC
AAATACGCTTTGAAGCCATGACTCT