DC900091

Overview
NameDC900091
Unique NameDC900091
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1042
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4107 contig Csv1_Contig4107:287..1325. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: UBL1_SCHPO (Ubiquitin-like protein 1 OS=Schizosaccharomyces pombe GN=ubl1 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 8.639e-17
Identity = 40/76 (52.63%), Postives = 56/76 (73.68%), Query Frame = 1
Query:  226 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          

HSP 2 Score: 88.1965 bits (217), Expect = 8.639e-17
Identity = 40/76 (52.63%), Postives = 56/76 (73.68%), Query Frame = 2
Query:  683 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 910
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          

HSP 3 Score: 83.5741 bits (205), Expect = 2.128e-15
Identity = 36/71 (50.70%), Postives = 53/71 (74.65%), Query Frame = 1
Query:   13 ETLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            +TLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    6 KTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          

HSP 4 Score: 69.3218 bits (168), Expect = 4.227e-13
Identity = 30/56 (53.57%), Postives = 44/56 (78.57%), Query Frame = 1
Query:  454 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGKDS 621
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D K++
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNA 56          

HSP 5 Score: 26.9498 bits (58), Expect = 4.227e-13
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = 2
Query:  611 ERTLADYNIQKESTLHLVLRLRGG 682
            ++    Y+++  S LHLVL LRGG
Sbjct:   53 DKNAESYHLEGGSVLHLVLALRGG 76          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: RUB1_YEAST (NEDD8-like protein RUB1 OS=Saccharomyces cerevisiae GN=RUB1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 2.128e-15
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 1
Query:  226 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          

HSP 2 Score: 83.5741 bits (205), Expect = 2.128e-15
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 2
Query:  683 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 910
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          

HSP 3 Score: 79.7221 bits (195), Expect = 3.073e-14
Identity = 37/71 (52.11%), Postives = 54/71 (76.06%), Query Frame = 1
Query:   13 ETLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 225
            +TLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    6 KTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          

HSP 4 Score: 65.0846 bits (157), Expect = 3.616e-11
Identity = 29/52 (55.77%), Postives = 42/52 (80.77%), Query Frame = 1
Query:  454 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED 609
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDD 52          

HSP 5 Score: 24.6386 bits (52), Expect = 3.616e-11
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 2
Query:  617 TLADYNIQKESTLHLVLRLRGG 682
            T+ D ++ +   LHLVL LRGG
Sbjct:   55 TVTDAHLVEGMQLHLVLTLRGG 76          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: UCRP_MOUSE (Ubiquitin cross-reactive protein OS=Mus musculus GN=Isg15 PE=1 SV=4)

HSP 1 Score: 80.4925 bits (197), Expect = 1.801e-14
Identity = 49/146 (33.56%), Postives = 77/146 (52.74%), Query Frame = 1
Query:   19 LTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLR-LRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            L G    + V +S T+  +K +I  K G+P  QQRL      L+DG TL+   +   ST+ LV++     + I V+   G +   EV  + T+D +K K+  +E +  DQ  L F G+ +ED   L +Y ++ + T+   LRLRGG
Sbjct:   10 LGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGG 155          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: UBD_HUMAN (Ubiquitin D OS=Homo sapiens GN=UBD PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 2.353e-14
Identity = 48/146 (32.88%), Postives = 77/146 (52.74%), Query Frame = 1
Query:   34 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFVKTL--TGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            +T +    D++  +K  ++ K  +P   Q L+   K L+  R+L+ Y I KE T+HL L++       + +F+       K   L+V  S ++  VKA I+ K GI P+ Q +   GK+LEDG+ +ADY I+K + L L     GG
Sbjct:   20 MTFDANPYDSVKKIKEHVRSKTKVPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGIRKGNLLFLACYCIGG 165          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: UCRP_HUMAN (Interferon-induced 17 kDa protein OS=Homo sapiens GN=ISG15 PE=1 SV=5)

HSP 1 Score: 75.0998 bits (183), Expect = 7.568e-13
Identity = 50/148 (33.78%), Postives = 79/148 (53.38%), Query Frame = 1
Query:   19 LTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF--AGKQLEDGRTLADYNIQKESTLHLVL-RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            L G    + + SS ++  +KA+I  K G+   QQRL    +G  L+D   LA   +   ST+ LV+ +    + I V+   G++ T EV  + T+ ++K ++   EG+  D   L F GK LED   L +Y ++  ST+ + LRLRGG
Sbjct:   10 LAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG 157          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: UBD_RAT (Ubiquitin D OS=Rattus norvegicus GN=Ubd PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.876e-12
Identity = 44/146 (30.14%), Postives = 72/146 (49.32%), Query Frame = 1
Query:   34 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL------RGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            +T +   SD +  +   I+ +  +    Q L+   K L+  R L+ Y I KE+T+HL L++         + +      G+   L V  S ++  VK  I++   +PP +Q +   GK+LEDG+ +ADYNI+  S L L     GG
Sbjct:   16 MTFDTTMSDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRALSSYGIDKENTIHLTLKVVKPSDEELPLSLVESGDEGQRHLLRVRRSSSVAQVKEMIENVTAVPPKKQIVNCNGKRLEDGKIMADYNIKSGSLLFLTAHCIGG 161          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: UBD_MOUSE (Ubiquitin D OS=Mus musculus GN=Ubd PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.435e-11
Identity = 44/148 (29.73%), Postives = 73/148 (49.32%), Query Frame = 1
Query:   28 KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFV--KTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            + +T E   +D +  +   I+ +  +    Q L+   K L+  R L+ Y I KE+T+HL L++       + +F+      G+   L V  S ++  VK  I+    + P +Q +   GK+LEDG+ +ADYNI+  S L L     GG
Sbjct:   15 RLMTFETTENDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRKLSSYGIDKETTIHLTLKVVKPSDEELPLFLVESKNEGQRHLLRVRRSSSVAQVKEMIESVTSVIPKKQVVNCNGKKLEDGKIMADYNIKSGSLLFLTTHCTGG 162          
BLAST of DC900091 vs. ExPASy Swiss-Prot
Match: ANUB1_HUMAN (AN1-type zinc finger and ubiquitin domain-containing protein 1 OS=Homo sapiens GN=ANUBL1 PE=2 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 9.252e-11
Identity = 36/76 (47.37%), Postives = 50/76 (65.79%), Query Frame = 1
Query:  226 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 453
            M++F++TLTG    L V   +T+ +VKAKI+  EGIP  +Q LI+   +LE+   L DYNI +  TL LVL +RGG
Sbjct:   28 MELFIETLTGTCFELRVSPFETVISVKAKIRRLEGIPICRQHLIWNNMELENDYCLNDYNISEGCTLKLVLAMRGG 103          

HSP 2 Score: 68.1662 bits (165), Expect = 9.252e-11
Identity = 36/76 (47.37%), Postives = 50/76 (65.79%), Query Frame = 2
Query:  683 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 910
            M++F++TLTG    L V   +T+ +VKAKI+  EGIP  +Q LI+   +LE+   L DYNI +  TL LVL +RGG
Sbjct:   28 MELFIETLTGTCFELRVSPFETVISVKAKIRRLEGIPICRQHLIWNNMELENDYCLNDYNISEGCTLKLVLAMRGG 103          
The following BLAST results are available for this feature:
BLAST of DC900091 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98
Match NameE-valueIdentityDescription
UBL1_SCHPO8.639e-1752.63Ubiquitin-like protein 1 OS=Schizosaccharomyces po... [more]
RUB1_YEAST2.128e-1552.63NEDD8-like protein RUB1 OS=Saccharomyces cerevisia... [more]
UCRP_MOUSE1.801e-1433.56Ubiquitin cross-reactive protein OS=Mus musculus G... [more]
UBD_HUMAN2.353e-1432.88Ubiquitin D OS=Homo sapiens GN=UBD PE=1 SV=2[more]
UCRP_HUMAN7.568e-1333.78Interferon-induced 17 kDa protein OS=Homo sapiens ... [more]
UBD_RAT2.876e-1230.14Ubiquitin D OS=Rattus norvegicus GN=Ubd PE=2 SV=1[more]
UBD_MOUSE2.435e-1129.73Ubiquitin D OS=Mus musculus GN=Ubd PE=2 SV=1[more]
ANUB1_HUMAN9.252e-1147.37AN1-type zinc finger and ubiquitin domain-containi... [more]

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Properties
Property NameValue
Genbank descriptionDC900091 VSS Citrus sinensis cDNA clone VS27V07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900091 ID=DC900091|Name=DC900091|organism=Citrus sinensis|type=EST|length=1042bp
AATTCGGCACGAGAAACCCTAACCGGGAAGACGATAACCCTCGAGGTTGA
GAGCTCAGACACGATCGATAACGTTAAGGCGAAGATTCAAGACAAGGAGG
GAATCCCCCCGGACCAGCAGAGGCTGATCTTTGCTGGCAAGCAACTGGAA
GACGGCCGCACCTTGGCTGATTACAATATCCAGAAGGAGTCAACCCTTCA
TCTCGTCCTCAGGCTCCGTGGTGGTATGCAGATCTTCGTCAAAACCCTAA
CTGGGAAGACGATCACTCTGGAGGTGGAGAGTTCGGATACGATTGATAAC
GTCAAGGCCAAGATCCAGGACAAAGAGGGGATACCGCCGGACCAGCAAAG
ATTGATCTTCGCCGGTAAGCAGTTGGAAGATGGCCGCACCTTGGCCGATT
ACAACATTCAGAAAGAGTCAACTCTCCACCTTGTTCTTCGTCTCAGGGGA
GGCATGCAGATATTTGTTAAGACCCTTACTGGGAAAACAATTACTTTGGA
AGTTGAGAGCTCAGACACAATTGACAATGTCAAGGCAAAAATCCAAGACA
AGGAAGGAATTCCACCGGATCAGCAGCGTTTGATCTTCGCTGGCAAACAG
CTTGAGGATGGAAAGGACTCTTGCGGATTATAACATCCAGAAGGAGTCGA
CCCTTCATCTGGTTCTGCGCCTGAGGGGTGGTATGCAGATATTTGTCAAG
ACCTTGACTGGGAAGACTATTACTCTGGAGGTGGAGAGCTCAGATACTAT
TGATAATGTTAAGGCAAAGATTCAGGACAAGGAAGGGATTCCACCAGATC
AGCAGAGGCTCATCTTTGCTGGGAAGCAGCTTGAGGATGGCCGTACTTTA
GCTGATTATAACATTCAGAAGGAGTCCACCCTCCATCTTGTGCTCCGCCT
TCGTGGTGGTGAATTCTAAGAATGTTATCTGCTTGCCGCCGTGTCTGTTC
TGCTCTGCTGTAGCAGTAATATTTATTTTTCATTTGCTAGTCCTGCTTCT
GTTTCAGTCTTTTTGTCACATCAAGTTTTATGTGGAAATGAA
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