CN185706

Overview
NameCN185706
Unique NameCN185706
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig6145 contig Csv1_Contig6145:33..745. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_SULAC (Superoxide dismutase [Fe] OS=Sulfolobus acidocaldarius GN=sod PE=1 SV=3)

HSP 1 Score: 96.6709 bits (239), Expect = 5.476e-27
Identity = 53/130 (40.77%), Postives = 70/130 (53.85%), Query Frame = 1 
Query:  322 GHVNHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYASDVYQK 711
            GH  H+I+W N+AP  +GGG+P   +L   I+  +GS +   Q  S    +L GSGW  L  D E   L + T  N    +     L  +L +D +EHAYYLQYKN + DYL   WNV+NW  A    QK
Sbjct:   81 GHKLHAIYWNNMAPAGKGGGKPG-GALADLINKQYGSFDRFKQVFSESANSLPGSGWTVLYYDNESGNLQIMTVENH--FMNHIAELPVILIVDEFEHAYYLQYKNKRGDYLNAWWNVVNWDDAEKRLQK 207

HSP 2 Score: 45.4394 bits (106), Expect = 5.476e-27
Identity = 22/67 (32.84%), Postives = 35/67 (52.24%), Query Frame = 2 
Query:  110 LQTVTLPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVEQLFQALNKVDTSTVVQLQGAIK 310
            L+    P LPY   ALEP IS +I+ +H+  HH+ YV   N  +++L + +          LQG ++
Sbjct:    7 LKRYEFPQLPYKVDALEPYISKDIIDVHYNGHHKGYVNGANSLLDRLEKLIKGDLPQGQYDLQGILR 73
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_SHIFL (Superoxide dismutase [Fe] OS=Shigella flexneri GN=sodB PE=3 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 5.490e-27
Identity = 47/122 (38.52%), Postives = 68/122 (55.74%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYAS 696
            NH+ +W  LAP    GGEP    +  AI   FGS      + +       GSGW WL  +++ K  +V T+    PL T A    PLL +DVWEHAYY+ Y+N +P YL++ W ++NW++ +
Sbjct:   73 NHTFYWNCLAP--NAGGEPT-GKVAEAIAASFGSFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDA---TPLLTVDVWEHAYYIDYRNARPGYLEHFWALVNWEFVA 188

HSP 2 Score: 48.1358 bits (113), Expect = 5.490e-27
Identity = 21/40 (52.50%), Postives = 26/40 (65.00%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVE 244
            LP LPY   AL P IS E ++ H+ KHHQ YVTN N  ++
Sbjct:    5 LPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK 44
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_ECOLI (Superoxide dismutase [Fe] OS=Escherichia coli (strain K12) GN=sodB PE=1 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 5.490e-27
Identity = 47/122 (38.52%), Postives = 68/122 (55.74%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYAS 696
            NH+ +W  LAP    GGEP    +  AI   FGS      + +       GSGW WL  +++ K  +V T+    PL T A    PLL +DVWEHAYY+ Y+N +P YL++ W ++NW++ +
Sbjct:   73 NHTFYWNCLAP--NAGGEPT-GKVAEAIAASFGSFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDA---TPLLTVDVWEHAYYIDYRNARPGYLEHFWALVNWEFVA 188

HSP 2 Score: 48.1358 bits (113), Expect = 5.490e-27
Identity = 21/40 (52.50%), Postives = 26/40 (65.00%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVE 244
            LP LPY   AL P IS E ++ H+ KHHQ YVTN N  ++
Sbjct:    5 LPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK 44
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_ECOL6 (Superoxide dismutase [Fe] OS=Escherichia coli O6 GN=sodB PE=3 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 5.490e-27
Identity = 47/122 (38.52%), Postives = 68/122 (55.74%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYAS 696
            NH+ +W  LAP    GGEP    +  AI   FGS      + +       GSGW WL  +++ K  +V T+    PL T A    PLL +DVWEHAYY+ Y+N +P YL++ W ++NW++ +
Sbjct:   73 NHTFYWNCLAP--NAGGEPT-GKVAEAIAASFGSFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDA---TPLLTVDVWEHAYYIDYRNARPGYLEHFWALVNWEFVA 188

HSP 2 Score: 48.1358 bits (113), Expect = 5.490e-27
Identity = 21/40 (52.50%), Postives = 26/40 (65.00%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVE 244
            LP LPY   AL P IS E ++ H+ KHHQ YVTN N  ++
Sbjct:    5 LPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK 44
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_ECO57 (Superoxide dismutase [Fe] OS=Escherichia coli O157:H7 GN=sodB PE=3 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 5.490e-27
Identity = 47/122 (38.52%), Postives = 68/122 (55.74%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYAS 696
            NH+ +W  LAP    GGEP    +  AI   FGS      + +       GSGW WL  +++ K  +V T+    PL T A    PLL +DVWEHAYY+ Y+N +P YL++ W ++NW++ +
Sbjct:   73 NHTFYWNCLAP--NAGGEPT-GKVAEAIAASFGSFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDA---TPLLTVDVWEHAYYIDYRNARPGYLEHFWALVNWEFVA 188

HSP 2 Score: 48.1358 bits (113), Expect = 5.490e-27
Identity = 21/40 (52.50%), Postives = 26/40 (65.00%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVE 244
            LP LPY   AL P IS E ++ H+ KHHQ YVTN N  ++
Sbjct:    5 LPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK 44
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_PSEAE (Superoxide dismutase [Fe] OS=Pseudomonas aeruginosa GN=sodB PE=3 SV=3)

HSP 1 Score: 89.7373 bits (221), Expect = 7.147e-27
Identity = 45/122 (36.89%), Postives = 68/122 (55.74%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYAS 696
            NH+ +W  L+P   GGG+P   +L  AI+  FGS +   ++ +       GSGW WL    +    +  T    +PL T   T  PLL  DVWEHAYY+ Y+N++P Y++  WN++NW + +
Sbjct:   73 NHTFYWNCLSP--NGGGQPT-GALADAINAAFGSFDKFKEEFTKTSVGTFGSGWGWLVKKADGSLALASTIGAGNPL-TSGDT--PLLTCDVWEHAYYIDYRNLRPKYVEAFWNLVNWDFVA 188

HSP 2 Score: 51.9878 bits (123), Expect = 7.147e-27
Identity = 21/39 (53.85%), Postives = 26/39 (66.67%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAV 241
            LP LPY+ +ALEP IS E ++ HH KHH  YV N N  +
Sbjct:    5 LPPLPYEKNALEPHISAETLEYHHDKHHNTYVVNLNNLI 43
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODM_KLEPL (Superoxide dismutase [Fe] (Fragment) OS=Klebsiella planticola GN=sodA PE=1 SV=3)

HSP 1 Score: 84.3445 bits (207), Expect = 1.216e-26
Identity = 43/101 (42.57%), Postives = 59/101 (58.42%), Query Frame = 1 
Query:  322 GHVNHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYY 624
            GH NH++FW+ +AP   GG   P   +  AID  FGS +A  ++ +       GSGW WL +D +   + V +T NQD  V +  T  P+LG+DVWEHAYY
Sbjct:   77 GHANHTLFWEVIAP---GGSNTPVGEVAKAIDAKFGSFDAFKEEFAKAATTRFGSGWAWLIVDGD--SVAVTSTPNQDSPVMEGKT--PVLGLDVWEHAYY 170

HSP 2 Score: 56.6102 bits (135), Expect = 1.216e-26
Identity = 24/40 (60.00%), Postives = 27/40 (67.50%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVE 244
            LP LPY Y ALEP I  +  ++HH KHH  YVTN N AVE
Sbjct:    5 LPQLPYAYDALEPHIDAKTXEIHHSKHHNTYVTNLNAAVE 44
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_PSEPK (Superoxide dismutase [Fe] OS=Pseudomonas putida (strain KT2440) GN=sodB PE=3 SV=3)

HSP 1 Score: 90.1225 bits (222), Expect = 2.050e-26
Identity = 45/126 (35.71%), Postives = 70/126 (55.56%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYASDVYQ 708
            NH+ +W  L+P   GGG+P   +L  AI+  FGS +   ++ +       GSGW WL    +    +  T     PL T   T  PLL  DVWEHAYY+ Y+N++P Y++  WN++NW + ++ ++
Sbjct:   73 NHTFYWNCLSP--NGGGQPT-GALADAINAAFGSFDKFKEEFTKTSVGTFGSGWGWLVKKADGSLALASTIGAGCPL-TSGDT--PLLTCDVWEHAYYIDYRNLRPKYVEAFWNLVNWAFVAEQFE 192

HSP 2 Score: 50.0618 bits (118), Expect = 2.050e-26
Identity = 21/39 (53.85%), Postives = 25/39 (64.10%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAV 241
            LP LPY + AL+P IS E ++ HH KHH  YV N N  V
Sbjct:    5 LPPLPYAHDALQPHISKETLEYHHDKHHNTYVVNLNNLV 43
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_PSEPU (Superoxide dismutase [Fe] OS=Pseudomonas putida GN=sodB PE=1 SV=3)

HSP 1 Score: 88.9669 bits (219), Expect = 3.474e-26
Identity = 45/126 (35.71%), Postives = 69/126 (54.76%), Query Frame = 1 
Query:  331 NHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYASDVYQ 708
            NH+ +W  LAP    GG+P   +L  AI+  FGS +   ++ +       GSGW WL    +    +  T     PL T   T  PLL  DVWEHAYY+ Y+N++P Y++  WN++NW + ++ ++
Sbjct:   73 NHTFYWNCLAP--NAGGQPT-GALADAINAAFGSFDKFKEEFTKTSVGTFGSGWGWLVKKADGSLALASTIGAGCPL-TSGDT--PLLTCDVWEHAYYIDYRNLRPKYVEAFWNLVNWAFVAEQFE 192

HSP 2 Score: 50.447 bits (119), Expect = 3.474e-26
Identity = 21/39 (53.85%), Postives = 25/39 (64.10%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAV 241
            LP LPY + AL+P IS E ++ HH KHH  YV N N  V
Sbjct:    5 LPPLPYAHDALQPHISKETLEFHHDKHHNTYVVNLNNLV 43
[Close]
BLAST of CN185706 vs. ExPASy Swiss-Prot
Match: SODF_SULSO (Superoxide dismutase [Fe] OS=Sulfolobus solfataricus GN=sod PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 5.877e-26
Identity = 49/130 (37.69%), Postives = 71/130 (54.62%), Query Frame = 1 
Query:  322 GHVNHSIFWKNLAPVHEGGGEPPHSSLGWAIDTHFGSLEALIQKMSAEGAALQGSGWVWLGLDTEFKRLVVETTANQDPLVTKAPTLVPLLGIDVWEHAYYLQYKNVKPDYLKNIWNVMNWKYASDVYQK 711
            GH  H+++W+N+AP  +GGG+P   +L   I+  +GS +   Q  +    +L G+GW  L  DTE   L + T  N          +  +L +D +EHAYYLQYKN + DY+   WNV+NW  A    QK
Sbjct:   81 GHKLHALYWENMAPSGKGGGKPG-GALADLINKQYGSFDRFKQVFTETANSLPGTGWAVLYYDTESGNLQIMTFENH--FQNHIAEIPIILILDEFEHAYYLQYKNKRADYVNAWWNVVNWDAAEKKLQK 207

HSP 2 Score: 47.3654 bits (111), Expect = 5.877e-26
Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 2 
Query:  125 LPDLPYDYSALEPAISGEIMQLHHQKHHQAYVTNYNKAVEQLFQALNKVDTSTVVQLQGAIK 310
            LP LPY   ALEP IS +I+ +H+  HH+ YV   N  +E+L + +     +    +QG I+
Sbjct:   12 LPPLPYKIDALEPYISKDIIDVHYNGHHKGYVNGANSLLERLEKVVKGDLQTGQYDIQGIIR 73
The following BLAST results are available for this feature:
BLAST of CN185706 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 221
Match NameE-valueIdentityDescription
SODF_SULAC5.476e-2740.77Superoxide dismutase [Fe] OS=Sulfolobus acidocalda... [more]
SODF_SHIFL5.490e-2738.52Superoxide dismutase [Fe] OS=Shigella flexneri GN=... [more]
SODF_ECOLI5.490e-2738.52Superoxide dismutase [Fe] OS=Escherichia coli (str... [more]
SODF_ECOL65.490e-2738.52Superoxide dismutase [Fe] OS=Escherichia coli O6 G... [more]
SODF_ECO575.490e-2738.52Superoxide dismutase [Fe] OS=Escherichia coli O157... [more]
SODF_PSEAE7.147e-2736.89Superoxide dismutase [Fe] OS=Pseudomonas aeruginos... [more]
SODM_KLEPL1.216e-2642.57Superoxide dismutase [Fe] (Fragment) OS=Klebsiella... [more]
SODF_PSEPK2.050e-2635.71Superoxide dismutase [Fe] OS=Pseudomonas putida (s... [more]
SODF_PSEPU3.474e-2635.71Superoxide dismutase [Fe] OS=Pseudomonas putida GN... [more]
SODF_SULSO5.877e-2637.69Superoxide dismutase [Fe] OS=Sulfolobus solfataric... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001M01_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001M01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185706 ID=CN185706; Name=CN185706; organism=Citrus sinensis; type=EST; length=712bp
TTTCGAACGACTCTTAAACGTCACCGTTTCATTCGCGATGGCTCTCCGTT
CGCTCGCCACCAGAAAAGCTATAGGCTTGGGTAAATCCGTAGGATTAGGG
CTACGTGGACTGCAGACGGTCACGCTGCCTGATCTTCCGTACGATTACAG
CGCTTTGGAGCCGGCGATTAGCGGAGAGATTATGCAGCTTCATCACCAGA
AGCATCACCAGGCTTACGTTACTAACTACAACAAGGCGGTCGAGCAGCTT
TTTCAAGCTTTGAACAAAGTCGACACTTCCACCGTCGTCCAGTTGCAGGG
CGCTATCAAGTTCAACGGCGAGGTCATGTCAACCACTCAATTTTCTGGAA
GAATCTTGCTCCTGTTCACGAAGGAGGTGGCGAGCCACCACATAGTTCCC
TTGGTTGGGCTATTGACACGCATTTTGGTTCTCTGGAAGCATTGATACAA
AAGATGAGTGCTGAGGGTGCAGCTTTGCAGGGCTCTGGATGGGTGTGGCT
TGGTTTAGATACAGAGTTTAAAAGGCTTGTGGTTGAAACTACTGCAAATC
AGGATCCACTGGTGACTAAAGCCCCAACTCTGGTTCCTTTGCTTGGTATA
GATGTTTGGGAGCATGCATACTACCTACAGTACAAGAATGTCAAACCAGA
TTATCTGAAAAACATTTGGAACGTAATGAACTGGAAGTATGCTAGTGATG
TGTATCAGAAAG
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