CN185577

Overview
NameCN185577
Unique NameCN185577
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length648
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN185577_ssr91 microsatellite CN185577_ssr91:91..102. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2493 contig Csv1_Contig2493:1..648. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_STAEQ (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=gnd PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 5.942e-21
Identity = 52/111 (46.85%), Postives = 72/111 (64.86%), Query Frame = 2 
Query:  314 RRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            ++IG+ GLAVMG+NLA NI  +G+ +SVYNR+  K DE V+ +      P Y     E FV S++KPR I+++VKAGP  D TI  L   ++  D +IDGGN  Y++T RR
Sbjct:    3 QQIGVVGLAVMGKNLAWNIESRGYSVSVYNRSRQKTDEMVKESPGREIYPTYSL---EEFVESLEKPRKILLMVKAGPATDATIDGLLPLLDDDDILIDGGNTNYQDTIRR 110
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_CANAL (6-phosphogluconate dehydrogenase, decarboxylating OS=Candida albicans GN=DOR14 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 5.942e-21
Identity = 51/109 (46.79%), Postives = 73/109 (66.97%), Query Frame = 2 
Query:  320 IGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            IGL GLAVMGQNL LN+A+ G+ +  YNRTT+KVD  +E   +  ++   G    +  V  +++PR I++LVKAG PVD+ I  L  Y+E+GD +IDGGN  + ++ RR
Sbjct:   31 IGLIGLAVMGQNLILNMADHGYTVVAYNRTTAKVDRFLENEAKGKSI--LGAHSIKELVDQLKRPRRIMLLVKAGAPVDEFINQLLPYLEEGDIIIDGGNSHFPDSNRR 137
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAW (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MW2) GN=gnd PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.760e-21
Identity = 51/111 (45.95%), Postives = 76/111 (68.47%), Query Frame = 2 
Query:  314 RRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            ++IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P Y     E FV+S++KPR I+++V+AG   D TI +L   ++ GD +IDGGN  Y++T RR
Sbjct:    3 QQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRR 110
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAS (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MSSA476) GN=gnd PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.760e-21
Identity = 51/111 (45.95%), Postives = 76/111 (68.47%), Query Frame = 2 
Query:  314 RRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            ++IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P Y     E FV+S++KPR I+++V+AG   D TI +L   ++ GD +IDGGN  Y++T RR
Sbjct:    3 QQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRR 110
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAR (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MRSA252) GN=gnd PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.760e-21
Identity = 51/111 (45.95%), Postives = 76/111 (68.47%), Query Frame = 2 
Query:  314 RRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            ++IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P Y     E FV+S++KPR I+++V+AG   D TI +L   ++ GD +IDGGN  Y++T RR
Sbjct:    3 QQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRR 110
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAN (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain N315) GN=gnd PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.760e-21
Identity = 51/111 (45.95%), Postives = 76/111 (68.47%), Query Frame = 2 
Query:  314 RRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            ++IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P Y     E FV+S++KPR I+++V+AG   D TI +L   ++ GD +IDGGN  Y++T RR
Sbjct:    3 QQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRR 110
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAC (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain COL) GN=gnd PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.760e-21
Identity = 51/111 (45.95%), Postives = 76/111 (68.47%), Query Frame = 2 
Query:  314 RRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            ++IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P Y     E FV+S++KPR I+++V+AG   D TI +L   ++ GD +IDGGN  Y++T RR
Sbjct:    3 QQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRR 110
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_SHIFL (6-phosphogluconate dehydrogenase, decarboxylating OS=Shigella flexneri GN=gnd PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.760e-21
Identity = 47/114 (41.23%), Postives = 77/114 (67.54%), Query Frame = 2 
Query:  305 MVQRRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            M +++IG+ G+AVMG+NLALNI  +G+ +S++NR+  K +E +     +   P Y  ++   FV S++ PR I+++VKAG   D  I +L  Y++KGD +IDGGN ++++T RR
Sbjct:    1 MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPGKKLAPYYTVKE---FVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTFFQDTIRR 111
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_AGGAC (6-phosphogluconate dehydrogenase, decarboxylating OS=Aggregatibacter actinomycetemcomitans GN=gnd PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.014e-20
Identity = 53/113 (46.90%), Postives = 73/113 (64.60%), Query Frame = 2 
Query:  308 VQRRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            V+  IG+ GLAVMGQNL LN+ + GF +  YNRTTSKVDE +E A +  N  + G    E   + ++KPR ++++V+AG  VD  I  L  ++E GD +IDGGN  Y +T RR
Sbjct:    3 VKGDIGVIGLAVMGQNLILNMNDHGFKVVAYNRTTSKVDEFLEGAAKGTN--IIGAYSLEDLANKLEKPRKVMLMVRAGEVVDHFIDALLPHLEAGDIIIDGGNSNYPDTNRR 113
[Close]
BLAST of CN185577 vs. ExPASy Swiss-Prot
Match: 6PGD_LACLM (6-phosphogluconate dehydrogenase, decarboxylating OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=gnd PE=1 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 1.324e-20
Identity = 52/114 (45.61%), Postives = 77/114 (67.54%), Query Frame = 2 
Query:  305 MVQRRIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKQEGNLPLYGFRDPESFVHSIQKPRVIIMLVKAGPPVDQTIKTLSVYMEKGDCVIDGGNEWYENTERR 646
            M Q   G+ G+AVMG+NLALN+  +G+ +++YNRTTSK +E V +  Q+ NL     +  E FV S++KPR I+++V+AG   D TIK+L   ++ GD +IDGGN  + +T RR
Sbjct:    1 MAQANFGVVGMAVMGKNLALNVESRGYTVAIYNRTTSKTEE-VYKEHQDKNLVFT--KTLEEFVGSLEKPRRIMLMVQAGAATDATIKSLLPLLDIGDILIDGGNTHFPDTMRR 111
The following BLAST results are available for this feature:
BLAST of CN185577 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 54
Match NameE-valueIdentityDescription
6PGD_STAEQ5.942e-2146.856-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_CANAL5.942e-2146.796-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAW7.760e-2145.956-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAS7.760e-2145.956-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAR7.760e-2145.956-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAN7.760e-2145.956-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAC7.760e-2145.956-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_SHIFL7.760e-2141.236-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_AGGAC1.014e-2046.906-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_LACLM1.324e-2045.616-phosphogluconate dehydrogenase, decarboxylating ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001I23_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001I23, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185577 ID=CN185577; Name=CN185577; organism=Citrus sinensis; type=EST; length=648bp
CGTATGAGAACCAAAAAGTTGGCGTATGGGTTGGATTTTGTCCTCTGAGT
CAGAGATCAACTACCGAAGCCACGAAGAGAAACTAAGACAAATAATAATA
ATAAAAGGTTAAAAACAATCCGCAGAGTTTGACCTGTACATTTACAACAA
CTAACAAGGGCTTAAGAGCCAGGCCAATTAATAAATAGACTACATTCAAT
CAAATTGTTTTTTTTTTTCGCAAAACCCAGATGCTCAAATTTCCTAAAGT
CAGTTCATTTTCAAGCATTCTTCGCAGGGTTGAATTTTTCTCAGGTGACT
AAAGATGGTCCAGAGAAGAATTGGCCTAGCTGGCCTTGCTGTCATGGGTC
AAAATCTTGCCCTCAACATTGCTGAGAAAGGATTCCCAATTTCTGTTTAC
AATCGGACGACCTCTAAAGTTGACGAGACTGTTGAACGAGCCAAACAAGA
AGGGAATCTGCCCTTATATGGTTTCCGTGATCCTGAATCCTTTGTTCATT
CAATCCAGAAACCTCGTGTTATAATCATGCTTGTTAAAGCTGGTCCTCCT
GTTGATCAAACAATTAAAACATTGTCTGTTTACATGGAGAAAGGAGATTG
TGTCATTGATGGTGGTAATGAATGGTATGAGAATACCGAGAGGAGACA
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