CN185553 | Citrus Genome Database

Overview


Name	CN185553
Unique Name	CN185553
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	682

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Pulp cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig3195	contig	Csv1_Contig3195:81..761.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: FAL1_CANGA (ATP-dependent RNA helicase FAL1 OS=Candida glabrata GN=FAL1 PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.519e-32
Identity = 40/63 (63.49%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  249 SFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            +F++M L+E LLRGIY+YGFE PSAIQ R I     G++VI QAQSGTGKTATF  G+LQ +D
Sbjct:   24 TFESMDLKEGLLRGIYSYGFEAPSAIQSRAITQIISGKDVIAQAQSGTGKTATFTIGMLQAID 86

HSP 2 Score: 76.6406 bits (187), Expect = 1.519e-32
Identity = 38/76 (50.00%), Postives = 56/76 (73.68%), Query Frame = 1

Query:  457 QALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVRED-QRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            QALVL+PTRELA QI KV+  LGDY+ VK +A  GG ++++D ++I + G QV  GTPGRV DM++R+ +   +++
Sbjct:   93 QALVLSPTRELAAQIGKVVTNLGDYMNVKAYAMTGGKTMKDDLKKIQKHGCQVISGTPGRVLDMIKRRLIETRHVQ 168

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: FAL1_KLULA (ATP-dependent RNA helicase FAL1 OS=Kluyveromyces lactis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.978e-32
Identity = 39/69 (56.52%), Postives = 49/69 (71.01%), Query Frame = 1

Query:  457 QALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSL 663
            QALVL+PTRELA Q E V+  LGDYL V  HAC GG ++++D + +    QV  GTPGRV DM++RQ L
Sbjct:   93 QALVLSPTRELASQSESVISNLGDYLNVTAHACTGGKALQQDIKKVSKNCQVVSGTPGRVLDMIKRQVL 161

HSP 2 Score: 80.1073 bits (196), Expect = 1.978e-32
Identity = 39/67 (58.21%), Postives = 50/67 (74.63%), Query Frame = 3

Query:  237 EVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            +V  +F++M L+ +LLRGIY YGFE PS+IQ R I     G++VI QAQSGTGKTATF  G+LQ +D
Sbjct:   20 KVSATFESMNLKPDLLRGIYFYGFEYPSSIQSRAISQIISGKDVIAQAQSGTGKTATFTIGLLQAID 86

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: FAL1_YEAST (ATP-dependent RNA helicase FAL1 OS=Saccharomyces cerevisiae GN=FAL1 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.775e-31
Identity = 39/67 (58.21%), Postives = 52/67 (77.61%), Query Frame = 3

Query:  237 EVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            +V  +F++M L+++LLRGIY+YGFE PS+IQ R I     G++VI QAQSGTGKTATF  G+LQ +D
Sbjct:   20 KVSSTFESMNLKDDLLRGIYSYGFEAPSSIQSRAITQIISGKDVIAQAQSGTGKTATFTIGLLQAID 86

HSP 2 Score: 72.4034 bits (176), Expect = 2.775e-31
Identity = 35/71 (49.30%), Postives = 51/71 (71.83%), Query Frame = 1

Query:  457 QALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVQVAVGTPGRVFDMLRRQSLR 666
            QAL+L+PTRELA QI +V++ LGDY+ V   A  GG ++++D + +Q  G Q   GTPGRV DM+++Q L+
Sbjct:   93 QALILSPTRELASQIGQVVKNLGDYMNVNAFAITGGKTLKDDLKKMQKHGCQAVSGTPGRVLDMIKKQMLQ 163

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: FAL1_YEAS7 (ATP-dependent RNA helicase FAL1 OS=Saccharomyces cerevisiae (strain YJM789) GN=FAL1 PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.775e-31
Identity = 39/67 (58.21%), Postives = 52/67 (77.61%), Query Frame = 3

Query:  237 EVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            +V  +F++M L+++LLRGIY+YGFE PS+IQ R I     G++VI QAQSGTGKTATF  G+LQ +D
Sbjct:   20 KVSSTFESMNLKDDLLRGIYSYGFEAPSSIQSRAITQIISGKDVIAQAQSGTGKTATFTIGLLQAID 86

HSP 2 Score: 72.4034 bits (176), Expect = 2.775e-31
Identity = 35/71 (49.30%), Postives = 51/71 (71.83%), Query Frame = 1

Query:  457 QALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVQVAVGTPGRVFDMLRRQSLR 666
            QAL+L+PTRELA QI +V++ LGDY+ V   A  GG ++++D + +Q  G Q   GTPGRV DM+++Q L+
Sbjct:   93 QALILSPTRELASQIGQVVKNLGDYMNVNAFAITGGKTLKDDLKKMQKHGCQAVSGTPGRVLDMIKKQMLQ 163

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: FAL1_VANPO (ATP-dependent RNA helicase FAL1 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) GN=FAL1 PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 7.977e-31
Identity = 39/67 (58.21%), Postives = 50/67 (74.63%), Query Frame = 3

Query:  237 EVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            ++  +F++M L+++LLRGIY YGFE PSAIQ R I     G +VI QAQSGTGKTATF  G+LQ +D
Sbjct:   20 KISPTFESMNLKDDLLRGIYGYGFEAPSAIQSRAITQIISGTDVIAQAQSGTGKTATFTIGMLQAID 86

HSP 2 Score: 72.4034 bits (176), Expect = 7.977e-31
Identity = 37/70 (52.86%), Postives = 50/70 (71.43%), Query Frame = 1

Query:  457 QALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVRED-QRILQAGVQVAVGTPGRVFDMLRRQSL 663
            QALVL+PTRELA QI +V+  LGDY+ V+ +A  GG ++++D  R+ + G QV  GTPGRV DM +R  L
Sbjct:   93 QALVLSPTRELASQINQVVSNLGDYMNVQSYAMTGGKTMKDDLNRMQKNGCQVVSGTPGRVLDMFKRHLL 162

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A_DROME (Eukaryotic initiation factor 4A OS=Drosophila melanogaster GN=eIF-4a PE=2 SV=3)

HSP 1 Score: 114.005 bits (284), Expect = 7.476e-25
Identity = 56/126 (44.44%), Postives = 78/126 (61.90%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  R     ++     GK    ++     +   + +CQAL+LAPTRELA QI++V+ ALG+Y+ V  HAC+GGT+VRED RIL++G  V VGTPGRV+DM+ R+ LR  YIK
Sbjct:   49 GFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIK 174

HSP 2 Score: 106.301 bits (264), Expect = 1.545e-22
Identity = 51/71 (71.83%), Postives = 59/71 (83.10%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            +++ EVYD+FD M LRE LLRGIY YGFEKPSAIQQR I+P  +GR+VI QAQSGTGKTATF   ILQQ+D
Sbjct:   23 STWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQID 93

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A_SCLS1 (ATP-dependent RNA helicase eIF4A OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) GN=tif1 PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.410e-23
Identity = 55/126 (43.65%), Postives = 79/126 (62.70%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    ++     L   + +CQAL+LAPTRELAQQI+KV+ A+GD++ V+ HAC+GGTSVR+D + LQ G QV VGTPGRV DM++R+ L+ D +K
Sbjct:   44 GFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKLDPNVKQCQALILAPTRELAQQIQKVVVAIGDFMNVECHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRFLKTDSMK 169

HSP 2 Score: 102.834 bits (255), Expect = 1.708e-21
Identity = 49/76 (64.47%), Postives = 59/76 (77.63%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGLVQ 452
            ++YDE  DSFD M L+  LLRG+YAYGFE+PSAIQQR I+P  KG +VI QAQSGTGKTATF   +LQ+LD  + Q
Sbjct:   18 SNYDETVDSFDTMNLKPELLRGVYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKLDPNVKQ 93

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A_BOTFB (ATP-dependent RNA helicase eIF4A OS=Botryotinia fuckeliana (strain B05.10) GN=tif1 PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.410e-23
Identity = 55/126 (43.65%), Postives = 79/126 (62.70%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    ++     L   + +CQAL+LAPTRELAQQI+KV+ A+GD++ V+ HAC+GGTSVR+D + LQ G QV VGTPGRV DM++R+ L+ D +K
Sbjct:   44 GFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKLDPNVKQCQALILAPTRELAQQIQKVVVAIGDFMNVECHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRFLKTDSMK 169

HSP 2 Score: 102.834 bits (255), Expect = 1.708e-21
Identity = 49/76 (64.47%), Postives = 59/76 (77.63%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGLVQ 452
            ++YDE  DSFD M L+  LLRG+YAYGFE+PSAIQQR I+P  KG +VI QAQSGTGKTATF   +LQ+LD  + Q
Sbjct:   18 SNYDETVDSFDTMNLKPELLRGVYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKLDPNVKQ 93

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A_NEUCR (ATP-dependent RNA helicase eIF4A OS=Neurospora crassa GN=tif-1 PE=3 SV=2)

HSP 1 Score: 109.383 bits (272), Expect = 1.841e-23
Identity = 54/126 (42.86%), Postives = 78/126 (61.90%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    ++     +   L  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGTSVR+D + LQ G QV VGTPGRV DM++R+ L+ D +K
Sbjct:   43 GFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPSLKACQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRFLKTDSMK 168

HSP 2 Score: 102.064 bits (253), Expect = 2.914e-21
Identity = 49/74 (66.22%), Postives = 58/74 (78.38%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++YDE  DSFD M L+  LLRGIYAYGFE+PSAIQQR I+P  KG +VI QAQSGTGKTATF   +LQ++D  L
Sbjct:   17 SNYDETVDSFDEMNLKPELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPSL 90

[Close]

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A_CHAGB (ATP-dependent RNA helicase eIF4A OS=Chaetomium globosum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.841e-23
Identity = 53/126 (42.06%), Postives = 78/126 (61.90%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    ++     +   +  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGTSVR+D + LQ G QV VGTPGRV DM++R+ L+ D +K
Sbjct:   43 GFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPTVKSCQALILAPTRELAQQIQKVVIAIGDFMNIECHACIGGTSVRDDMKALQDGPQVIVGTPGRVHDMIQRRILKTDQMK 168

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 48/71 (67.61%), Postives = 57/71 (80.28%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            ++YDE  DSFD M L+  LLRGIYAYGFE+PSAIQQR I+P  KG +VI QAQSGTGKTATF   +LQ++D
Sbjct:   17 SNYDETVDSFDDMNLKSELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKID 87

The following BLAST results are available for this feature:

BLAST of CN185553 vs. ExPASy Swiss-Prot

BLAST of CN185553 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 478


Match Name	E-value	Identity	Description
FAL1_CANGA	1.519e-32	63.49	ATP-dependent RNA helicase FAL1 OS=Candida glabrat...	[more]
FAL1_KLULA	1.978e-32	56.52	ATP-dependent RNA helicase FAL1 OS=Kluyveromyces l...	[more]
FAL1_YEAST	2.775e-31	58.21	ATP-dependent RNA helicase FAL1 OS=Saccharomyces c...	[more]
FAL1_YEAS7	2.775e-31	58.21	ATP-dependent RNA helicase FAL1 OS=Saccharomyces c...	[more]
FAL1_VANPO	7.977e-31	58.21	ATP-dependent RNA helicase FAL1 OS=Vanderwaltozyma...	[more]
IF4A_DROME	7.476e-25	44.44	Eukaryotic initiation factor 4A OS=Drosophila mela...	[more]
IF4A_SCLS1	1.410e-23	43.65	ATP-dependent RNA helicase eIF4A OS=Sclerotinia sc...	[more]
IF4A_BOTFB	1.410e-23	43.65	ATP-dependent RNA helicase eIF4A OS=Botryotinia fu...	[more]
IF4A_NEUCR	1.841e-23	42.86	ATP-dependent RNA helicase eIF4A OS=Neurospora cra...	[more]
IF4A_CHAGB	1.841e-23	42.06	ATP-dependent RNA helicase eIF4A OS=Chaetomium glo...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS05_0001I07_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001I07, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN185553 ID=CN185553; Name=CN185553; organism=Citrus sinensis; type=EST; length=682bp
GCAGGCCGAGGAGTTTGGTAACCCTAGCAACTATTTTCTCTTAGATCAAT
CTTCTCCTTCTCTTTGAAGAAAAAAGAAAACCAACTCTTTCTCTCGATTT
TAATCGCCAGCATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAG
GATCTCAATTTGATGCTCGTCAGTATGACACCCAGATGAATGAACTGCTC
TCTACTGATGGGCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAG
TTTTGATGCAATGGGACTGCGGGAAAACCTTTTGAGGGGAATCTATGCCT
ATGGGTTTGAGAAGCCCTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTC
TGCAAGGGACGTGAGGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAAC
AGCAACTTTCTGCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCC
AAGTGTCAGGCCCTGGTATTGGCACCCACTAGGGAACTAGCTCAACAGAT
TGAGAAAGTAATGAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATG
CTTGTGTCGGTGGGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCT
GGTGTTCAGGTTGCTGTTGGCACTCCGGGTCGTGTGTTTGACATGCTGCG
CCGACAGTCTTTACGCCCTGATTACATTAAGA