CN185553 | Citrus Genome Database

Overview


Name	CN185553
Unique Name	CN185553
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	682

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Pulp cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig3195	contig	Csv1_Contig3195:81..761.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A1_RABIT (Eukaryotic initiation factor 4A-I (Fragment) OS=Oryctolagus cuniculus GN=EIF4A1 PE=1 SV=2)

HSP 1 Score: 107.071 bits (266), Expect = 9.138e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     + + L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++      + VGTPGRVFDML R+ L P YIK
Sbjct:   43 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIK 169

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 50/74 (67.57%), Postives = 60/74 (81.08%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQ++  L
Sbjct:   17 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDL 90

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A1_PANTR (Eukaryotic initiation factor 4A-I OS=Pan troglodytes GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.138e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     + + L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++      + VGTPGRVFDML R+ L P YIK
Sbjct:   51 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIK 177

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 50/74 (67.57%), Postives = 60/74 (81.08%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQ++  L
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDL 98

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A1_MOUSE (Eukaryotic initiation factor 4A-I OS=Mus musculus GN=Eif4a1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.138e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     + + L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++      + VGTPGRVFDML R+ L P YIK
Sbjct:   51 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIK 177

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 50/74 (67.57%), Postives = 60/74 (81.08%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQ++  L
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDL 98

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A1_MACFA (Eukaryotic initiation factor 4A-I OS=Macaca fascicularis GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.138e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     + + L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++      + VGTPGRVFDML R+ L P YIK
Sbjct:   51 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIK 177

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 50/74 (67.57%), Postives = 60/74 (81.08%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQ++  L
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDL 98

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A1_HUMAN (Eukaryotic initiation factor 4A-I OS=Homo sapiens GN=EIF4A1 PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.138e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     + + L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++      + VGTPGRVFDML R+ L P YIK
Sbjct:   51 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIK 177

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 50/74 (67.57%), Postives = 60/74 (81.08%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQ++  L
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDL 98

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A1_BOVIN (Eukaryotic initiation factor 4A-I OS=Bos taurus GN=EIF4A1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 9.138e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVR-EDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     + + L   QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR E Q++      + VGTPGRVFDML R+ L P YIK
Sbjct:   51 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIK 177

HSP 2 Score: 101.679 bits (252), Expect = 3.806e-21
Identity = 50/74 (67.57%), Postives = 60/74 (81.08%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++++E+ DSFD M L E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQ++  L
Sbjct:   25 SNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDL 98

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: DHH1_MAGGR (ATP-dependent RNA helicase DHH1 OS=Magnaporthe grisea GN=DHH1 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.070e-22
Identity = 33/67 (49.25%), Postives = 45/67 (67.16%), Query Frame = 1

Query:  445 LSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDM 645
            +SK Q L+L PTRELA Q  +V + LG +LG+ V    GGT++R+D   LQ  V + VGTPGR+ D+
Sbjct:  102 VSKIQCLILVPTRELAMQTSQVCKTLGKHLGINVMVTTGGTTLRDDILRLQDPVHIVVGTPGRILDL 168

HSP 2 Score: 57.7658 bits (138), Expect = 1.070e-22
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 3

Query:  252 FDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            F+  GL+ +LL GI+  GFEKPS IQ+  I     GR+++ +A++GTGKTA F    L+ ++
Sbjct:   38 FENFGLKRDLLMGIFEAGFEKPSPIQEESIPVALTGRDILARAKNGTGKTAAFVVPALETIN 99

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A_COCIM (ATP-dependent RNA helicase eIF4A OS=Coccidioides immitis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.193e-22
Identity = 54/126 (42.86%), Postives = 77/126 (61.11%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    ++     +   L  CQAL+LAPTRELAQQI+KV+ A+GD++ V+ HAC+GGT+VRED + L  G QV VGTPGRV DM++R+ L+ D +K
Sbjct:   44 GFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNLKACQALILAPTRELAQQIQKVVVAIGDFMSVECHACIGGTNVREDIKALNDGPQVVVGTPGRVHDMIQRRVLKTDQMK 169

HSP 2 Score: 103.605 bits (257), Expect = 1.001e-21
Identity = 50/74 (67.57%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLDYGL 446
            ++YDE+ DSFDAM LR  LLRG+YAYGFE+PSAIQQR I+P  KG +VI QAQSGTGKTATF    LQ++D  L
Sbjct:   18 SNYDEITDSFDAMNLRAELLRGVYAYGFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNL 91

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A2_RAT (Eukaryotic initiation factor 4A-II OS=Rattus norvegicus GN=Eif4a2 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.193e-22
Identity = 57/127 (44.88%), Postives = 75/127 (59.06%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     L +   + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA    + VGTPGRVFDML R+ L P +IK
Sbjct:   52 GFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIK 178

HSP 2 Score: 102.449 bits (254), Expect = 2.231e-21
Identity = 49/71 (69.01%), Postives = 60/71 (84.51%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQL+
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLE 96

BLAST of CN185553 vs. ExPASy Swiss-Prot
Match: IF4A2_PONAB (Eukaryotic initiation factor 4A-II OS=Pongo abelii GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.193e-22
Identity = 57/127 (44.88%), Postives = 75/127 (59.06%), Query Frame = 1

Query:  304 GLRSPLLFSKGELFPSARDVRSFSRLSLELGKQQLSALEFCSNLIMVLSKCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVQVAVGTPGRVFDMLRRQSLRPDYIK 681
            G   P    +  + P  +     ++     GK    A+     L +   + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA    + VGTPGRVFDML R+ L P +IK
Sbjct:   52 GFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIK 178

HSP 2 Score: 102.449 bits (254), Expect = 2.231e-21
Identity = 49/71 (69.01%), Postives = 60/71 (84.51%), Query Frame = 3

Query:  225 TSYDEVYDSFDAMGLRENLLRGIYAYGFEKPSAIQQRGIVPFCKGREVIQQAQSGTGKTATFCSGILQQLD 437
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG +VI QAQSGTGKTATF   ILQQL+
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLE 96

The following BLAST results are available for this feature:

BLAST of CN185553 vs. ExPASy Swiss-Prot

BLAST of CN185553 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 478


Match Name	E-value	Identity	Description
IF4A1_RABIT	9.138e-23	44.88	Eukaryotic initiation factor 4A-I (Fragment) OS=Or...	[more]
IF4A1_PANTR	9.138e-23	44.88	Eukaryotic initiation factor 4A-I OS=Pan troglodyt...	[more]
IF4A1_MOUSE	9.138e-23	44.88	Eukaryotic initiation factor 4A-I OS=Mus musculus ...	[more]
IF4A1_MACFA	9.138e-23	44.88	Eukaryotic initiation factor 4A-I OS=Macaca fascic...	[more]
IF4A1_HUMAN	9.138e-23	44.88	Eukaryotic initiation factor 4A-I OS=Homo sapiens ...	[more]
IF4A1_BOVIN	9.138e-23	44.88	Eukaryotic initiation factor 4A-I OS=Bos taurus GN...	[more]
DHH1_MAGGR	1.070e-22	49.25	ATP-dependent RNA helicase DHH1 OS=Magnaporthe gri...	[more]
IF4A_COCIM	1.193e-22	42.86	ATP-dependent RNA helicase eIF4A OS=Coccidioides i...	[more]
IF4A2_RAT	1.193e-22	44.88	Eukaryotic initiation factor 4A-II OS=Rattus norve...	[more]
IF4A2_PONAB	1.193e-22	44.88	Eukaryotic initiation factor 4A-II OS=Pongo abelii...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS05_0001I07_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001I07, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN185553 ID=CN185553; Name=CN185553; organism=Citrus sinensis; type=EST; length=682bp
GCAGGCCGAGGAGTTTGGTAACCCTAGCAACTATTTTCTCTTAGATCAAT
CTTCTCCTTCTCTTTGAAGAAAAAAGAAAACCAACTCTTTCTCTCGATTT
TAATCGCCAGCATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAG
GATCTCAATTTGATGCTCGTCAGTATGACACCCAGATGAATGAACTGCTC
TCTACTGATGGGCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAG
TTTTGATGCAATGGGACTGCGGGAAAACCTTTTGAGGGGAATCTATGCCT
ATGGGTTTGAGAAGCCCTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTC
TGCAAGGGACGTGAGGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAAC
AGCAACTTTCTGCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCC
AAGTGTCAGGCCCTGGTATTGGCACCCACTAGGGAACTAGCTCAACAGAT
TGAGAAAGTAATGAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATG
CTTGTGTCGGTGGGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCT
GGTGTTCAGGTTGCTGTTGGCACTCCGGGTCGTGTGTTTGACATGCTGCG
CCGACAGTCTTTACGCCCTGATTACATTAAGA