CN185528

Overview
NameCN185528
Unique NameCN185528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP5_ARATH (Polyadenylate-binding protein 5 OS=Arabidopsis thaliana GN=PAB5 PE=1 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 3.254e-13
Identity = 48/168 (28.57%), Postives = 87/168 (51.79%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++GDL P + ES++  +F       + +V R+  T+ S GY ++ F +   A R +++ N    P  ++  R+  +      R    G    +F+ +L A + +  L ETF + + ++   KV  D   GRSKGYGFV+F  E     ++ ++NG+L + + + +G
Sbjct:   46 SLYVGDLDPSVNESHLLDLFNQVAPVHNLRVCRDL-THRSLGYAYVNFANPEDASRAMESLN--YAPIRDRPIRIMLSNRDPSTRLSGKG---NVFIKNLDASIDNKALYETFSS-FGTILSCKVAMD-VVGRSKGYGFVQFEKEETAQAAIDKLNGMLLNDKQVFVG 205          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP4_HUMAN (Polyadenylate-binding protein 4 OS=Homo sapiens GN=PABPC4 PE=1 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.254e-13
Identity = 53/175 (30.29%), Postives = 87/175 (49.71%), Query Frame = 2
Query:  101 ASSDGIRSLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            ASS  + SL++GDL   + E+ +   F   G  +S +V R+  T  S GY ++ F   A AER L T N   +    +  R+ W+      R+   G    +F+ +L   + +  L +TF A + ++   KVV D     SKGY FV F  +    +++ +MNG+L + R + +G
Sbjct:    5 ASSYPMASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKG--KPIRIMWSQRDPSLRKSGVG---NVFIKNLDKSIDNKALYDTFSA-FGNILSCKVVCDE--NGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVG 171          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_LODEL (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Lodderomyces elongisporus GN=PAB1 PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.250e-13
Identity = 48/168 (28.57%), Postives = 84/168 (50.00%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++G+L P + E+ +  IF   G+  S +V R+  +  S GY ++ +      E+ ++  N T  P   +  R+ W+      RR  DG    IF+ +L   + +  L +TF A +  +   KV TD   G+SK +GFV +  E     ++  +NG+L + R + +G
Sbjct:   68 SLYVGELNPSVNEATLFEIFSPIGQVASIRVCRDAVSKKSLGYAYVNYHKLEDGEKAIEELNYT--PVEGRPCRIMWSQRDPSARRSGDG---NIFIKNLHPAIDNKALHDTFSA-FGKILSVKVATD-DLGQSKCFGFVHYETEEAAQAAIESVNGMLLNDREVYVG 228          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: ELAV4_HUMAN (ELAV-like protein 4 OS=Homo sapiens GN=ELAVL4 PE=1 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 4.250e-13
Identity = 45/163 (27.61%), Postives = 84/163 (51.53%), Query Frame = 2
Query:  104 SSDGIRSLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNG 592
            + D   +L +  L   M +    S+FG  GE  S K++R+K T  S GYGF+ ++    AE+ + T NG ++ +  +  ++++A   +   R     D  ++V  L   +T   L++ F + Y  +  ++++ D+ TG S+G GF+RF    E   ++  +NG
Sbjct:   41 TDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQT--KTIKVSYARPSSASIR-----DANLYVSGLPKTMTQKELEQLF-SQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNG 195          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_KLULA (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Kluyveromyces lactis GN=PAB1 PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.551e-13
Identity = 45/159 (28.30%), Postives = 82/159 (51.57%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVL 598
            SL++G+L P + E+ +  +F   G   S +V R+  T  S GY ++ +  + A ++ +Q  N  ++    +  R+ W+      R++  G    IF+ +L   + +  L ETF + +  V   KV  D   G S+G+GFV F +ES+   ++  +NG+L
Sbjct:   51 SLYVGELDPNITEALLYDVFSPLGPISSIRVCRDAVTKASLGYAYVNYTDYEAGKKAIQELNYAEI--NGRPCRIMWSERDPAIRKKGSG---NIFIKNLHPAIDNKALHETF-STFGEVLSCKVALDE-NGNSRGFGFVHFKEESDAKDAIEAVNGML 202          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: ELAV2_RAT (ELAV-like protein 2 OS=Rattus norvegicus GN=Elavl2 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.551e-13
Identity = 42/147 (28.57%), Postives = 79/147 (53.74%), Query Frame = 2
Query:  152 MEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNG 592
            M +  + S+FG  GE  S K++R+K T  S GYGF+ ++    AE+ + T NG ++ +  +  ++++A   +   R     D  ++V  L   +T   L++ F + Y  +  ++++ D+ TG S+G GF+RF    E   ++  +NG
Sbjct:   50 MTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQT--KTIKVSYARPSSASIR-----DANLYVSGLPKTMTQKELEQLF-SQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNG 188          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: RBM42_RAT (RNA-binding protein 42 OS=Rattus norvegicus GN=Rbm42 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.250e-13
Identity = 37/79 (46.84%), Postives = 51/79 (64.56%), Query Frame = 2
Query:  386 DGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            D  DF IF GDL  +V D +L   F    S +K AKV+ D+ TG++KGYGFV F D S+ +R+M EMNG    +RP+++
Sbjct:  371 DADDFRIFCGDLGNEVNDDILARAFSRFPSFLK-AKVIRDKRTGKTKGYGFVSFKDPSDYVRAMREMNGKYVGSRPIKL 448          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: RBM42_MOUSE (RNA-binding protein 42 OS=Mus musculus GN=Rbm42 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.250e-13
Identity = 37/79 (46.84%), Postives = 51/79 (64.56%), Query Frame = 2
Query:  386 DGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            D  DF IF GDL  +V D +L   F    S +K AKV+ D+ TG++KGYGFV F D S+ +R+M EMNG    +RP+++
Sbjct:  371 DADDFRIFCGDLGNEVNDDILARAFSRFPSFLK-AKVIRDKRTGKTKGYGFVSFKDPSDYVRAMREMNGKYVGSRPIKL 448          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: RBM42_HUMAN (RNA-binding protein 42 OS=Homo sapiens GN=RBM42 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.250e-13
Identity = 37/79 (46.84%), Postives = 51/79 (64.56%), Query Frame = 2
Query:  386 DGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            D  DF IF GDL  +V D +L   F    S +K AKV+ D+ TG++KGYGFV F D S+ +R+M EMNG    +RP+++
Sbjct:  377 DADDFRIFCGDLGNEVNDDILARAFSRFPSFLK-AKVIRDKRTGKTKGYGFVSFKDPSDYVRAMREMNGKYVGSRPIKL 454          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: RBM42_BOVIN (RNA-binding protein 42 OS=Bos taurus GN=RBM42 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.250e-13
Identity = 37/79 (46.84%), Postives = 51/79 (64.56%), Query Frame = 2
Query:  386 DGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            D  DF IF GDL  +V D +L   F    S +K AKV+ D+ TG++KGYGFV F D S+ +R+M EMNG    +RP+++
Sbjct:  345 DADDFRIFCGDLGNEVNDDILARAFSRFPSFLK-AKVIRDKRTGKTKGYGFVSFKDPSDYVRAMREMNGKYVGSRPIKL 422          
The following BLAST results are available for this feature:
BLAST of CN185528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 107
Match NameE-valueIdentityDescription
PABP5_ARATH3.254e-1328.57Polyadenylate-binding protein 5 OS=Arabidopsis tha... [more]
PABP4_HUMAN3.254e-1330.29Polyadenylate-binding protein 4 OS=Homo sapiens GN... [more]
PABP_LODEL4.250e-1328.57Polyadenylate-binding protein, cytoplasmic and nuc... [more]
ELAV4_HUMAN4.250e-1327.61ELAV-like protein 4 OS=Homo sapiens GN=ELAVL4 PE=1... [more]
PABP_KLULA5.551e-1328.30Polyadenylate-binding protein, cytoplasmic and nuc... [more]
ELAV2_RAT5.551e-1328.57ELAV-like protein 2 OS=Rattus norvegicus GN=Elavl2... [more]
RBM42_RAT7.250e-1346.84RNA-binding protein 42 OS=Rattus norvegicus GN=Rbm... [more]
RBM42_MOUSE7.250e-1346.84RNA-binding protein 42 OS=Mus musculus GN=Rbm42 PE... [more]
RBM42_HUMAN7.250e-1346.84RNA-binding protein 42 OS=Homo sapiens GN=RBM42 PE... [more]
RBM42_BOVIN7.250e-1346.84RNA-binding protein 42 OS=Bos taurus GN=RBM42 PE=2... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001H18_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001H18, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185528 ID=CN185528; Name=CN185528; organism=Citrus sinensis; type=EST; length=625bp
GCAACAACATCAAACTCCGGTGCCACCTCCGGTTGGATGGACTCCGCAGC
CGGTGCCTCCTCCATCTCAGCAGACGCAGCCCTACGGTGTCGCCCCCGAT
GCTTCCTCCGACGGGATCCGTTCTTTGTGGATCGGAGACTTGCAGCCGTG
GATGGAGGAGAGTTATATCGCCAGCATCTTCGGCCATACTGGAGAGTTTG
TTTCAGGAAAGGTTATTCGCAACAAGCAAACAAATTTTTCAGAGGGTTAT
GGTTTTATTGAGTTTGTTAGTCATGCTGCTGCAGAGAGGGTTTTGCAGAC
TTTTAATGGCACCCAAATGCCAAGCACTGAGCAAAACTTCAGACTGAATT
GGGCAACCTATGGGGCTGGTGAGAGGCGTCAAGATGATGGTCCTGATTTT
ACAATTTTTGTGGGAGATTTGGCTGCCGATGTAACTGACTATGTGCTACA
AGAAACATTTAGAGCTGTCTACTCGTCAGTGAAGGGTGCTAAAGTGGTCA
CTGATAGAACCACTGGACGCTCTAAGGGGTATGGTTTTGTGAGATTTGGG
GATGAAAGTGAACAATTGCGTTCCATGACCGAAATGAATGGCGTGCTCTG
CTCTACTAGACCTATGCGGATTGGG
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