CN185528

Overview
NameCN185528
Unique NameCN185528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_COCIM (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Coccidioides immitis GN=PAB1 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.250e-13
Identity = 46/168 (27.38%), Postives = 84/168 (50.00%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++G+L P + E+ +  +F   G+  S +V R+  T  S GY ++ + + A  ER L+  N T +    +  R+ W+      R+   G    +F+ +L   + +  L +TF A + ++   KV  D   G SKGYGFV +        ++  +NG+L + + + +G
Sbjct:   56 SLYVGELDPSVTEAMLFELFSSIGQVASIRVCRDAVTRRSLGYAYVNYNNTADGERALEDLNYTLIKG--RPCRIMWSQRDPALRKTGQG---NVFIKNLDTAIDNKALHDTF-AAFGNILSCKVAQDE-FGNSKGYGFVHYETAEAAQNAIKHVNGMLLNDKKVFVG 216          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_ASPCL (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Aspergillus clavatus GN=pab1 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.250e-13
Identity = 46/168 (27.38%), Postives = 85/168 (50.60%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++G+L P + E+ +  +F   G+  S +V R+  T  S GY ++ + + A  ER L+  N T +    +  R+ W+      R+   G    +F+ +L + + +  L +TF A + ++   KV  D   G SKGYGFV +        ++  +NG+L + + + +G
Sbjct:   52 SLYVGELDPSVTEAMLYELFSSIGQVASIRVCRDAVTRRSLGYAYVNYNNTADGERALEDLNYTLIKG--KPCRIMWSQRDPALRKTGQG---NVFIKNLDSAIDNKALHDTF-AAFGNILSCKVAQDE-FGNSKGYGFVHYETAEAANNAIKHVNGMLLNDKKVFVG 212          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_USTMA (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Ustilago maydis GN=PAB1 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 9.468e-13
Identity = 48/168 (28.57%), Postives = 85/168 (50.60%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++G+L P + E+ +  IF   G   S +V R+  T  S GY ++ F++ A  ER ++  N + +    +  R+ W+      RR   G    IF+ +L A + +  L +TF A + ++   KV T+ T   S GYGFV +        ++  +NG+L + + + +G
Sbjct:   48 SLYVGELDPSVTEAMLFEIFSMIGTVASIRVCRDAVTRRSLGYAYVNFLNAADGERAMEQLNYSLI--RNRPCRIMWSQRDPALRRTGQG---NIFIKNLDAGIDNKALHDTF-AAFGNILSCKVATNET--GSLGYGFVHYETAEAAEAAIKHVNGMLLNDKKVYVG 207          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: ELAV1_MOUSE (ELAV-like protein 1 OS=Mus musculus GN=Elavl1 PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.237e-12
Identity = 48/169 (28.40%), Postives = 87/169 (51.48%), Query Frame = 2
Query:   89 VAPDASSD-GIRSLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNG 592
            +A D   D G  +L +  L   M +  + S+F   GE  S K+IR+K    S GYGF+ +V+   AER + T NG ++ S  +  ++++A   +   +     D  +++  L   +T   +++ F + +  +  ++V+ D+TTG S+G  F+RF   SE   ++T   G
Sbjct:    9 MAEDCRGDIGRTNLIVNYLPQNMTQEELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAISTLNGLRLQS--KTIKVSYARPSSEVIK-----DANLYISGLPRTMTQKDVEDMF-SRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFIG 169          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: ROC3_ARATH (31 kDa ribonucleoprotein, chloroplastic OS=Arabidopsis thaliana GN=RBP31 PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.615e-12
Identity = 53/182 (29.12%), Postives = 90/182 (49.45%), Query Frame = 2
Query:   95 PDASSDGIRSLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDG------PDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            P+ S +    L++G+L   +    +A +F   G     +VI N++T+ S G+GF+   S   AE  ++ FN   +     N RL      A    + +       P F ++VG+L  DV +  L++ F + +  V  A+VV DR TGRS+G+GFV   D  E   +++ ++G     R +R+
Sbjct:  144 PEPSEEA--KLFVGNLAYDVNSQALAMLFEQAGTVEIAEVIYNRETDQSRGFGFVTMSSVDEAETAVEKFNRYDL-----NGRLLTVNKAAPRGSRPERAPRVYEPAFRVYVGNLPWDVDNGRLEQLF-SEHGKVVEARVVYDRETGRSRGFGFVTMSDVDELNEAISALDGQNLEGRAIRV 317          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_CRYNE (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Cryptococcus neoformans GN=PAB1 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.615e-12
Identity = 46/168 (27.38%), Postives = 85/168 (50.60%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++G+L P + E+ +  IF   G   S +V R+  T  S GY ++ +++ A  ER L+  N + +    Q+ R+ W+      R+   G    IF+ +L   + +  L +TF A +  +   KV TD   G+S+G+ FV +        ++  +NG+L + + + +G
Sbjct:   47 SLYVGELDPSVTEAMLFEIFNMIGPVASIRVCRDAVTRRSLGYAYVNYLNAADGERALEHLNYSLIKG--QSCRIMWSQRDPALRKTGQG---NIFIKNLDQSIDNKALHDTF-AAFGDILSCKVGTDE-NGKSRGFAFVHYSTGEAADAAIKAVNGMLLNDKKVYVG 207          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: PABP_CHAGB (Polyadenylate-binding protein, cytoplasmic and nuclear OS=Chaetomium globosum GN=PAB1 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.615e-12
Identity = 44/168 (26.19%), Postives = 84/168 (50.00%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRIG 625
            SL++G+L P + E+ +  +F   G   S +V R+  T  S GY ++ + S +  E+ L+  N T +    +  R+ W+      R+   G    +F+ +L   + +  L +TF A + ++   KV  D   G SKGYGFV +  +    +++  +N +L + + + +G
Sbjct:   66 SLYVGELDPSVTEAMLFELFSQIGSVASIRVCRDTITRRSLGYAYVNYNSTSDGEKALEELNYTLIKG--RPCRIMWSQRDPALRKTGQG---NVFIKNLDVAIDNKALHDTF-AAFGNILSCKVAQDE-NGNSKGYGFVHYETDEAAAQAIKHVNNMLLNEKKVYVG 226          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: SF3B4_RAT (Splicing factor 3B subunit 4 OS=Rattus norvegicus GN=Sf3b4 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.109e-12
Identity = 45/167 (26.95%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            ++++G L   + E  +  +F   G  V+  + +++ T   +GYGF+EF+S   A+  ++  N  ++    +  R+N A+  A  +  D G +  IF+G+L  ++ + +L +TF A    ++  K++ D  TG SKGY F+ F        ++  MNG     RP+ +
Sbjct:   14 TVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYG--KPIRVNKAS--AHNKNLDVGAN--IFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITV 174          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: SF3B4_MOUSE (Splicing factor 3B subunit 4 OS=Mus musculus GN=Sf3b4 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.109e-12
Identity = 45/167 (26.95%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            ++++G L   + E  +  +F   G  V+  + +++ T   +GYGF+EF+S   A+  ++  N  ++    +  R+N A+  A  +  D G +  IF+G+L  ++ + +L +TF A    ++  K++ D  TG SKGY F+ F        ++  MNG     RP+ +
Sbjct:   14 TVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYG--KPIRVNKAS--AHNKNLDVGAN--IFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITV 174          
BLAST of CN185528 vs. ExPASy Swiss-Prot
Match: SF3B4_HUMAN (Splicing factor 3B subunit 4 OS=Homo sapiens GN=SF3B4 PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.109e-12
Identity = 45/167 (26.95%), Postives = 86/167 (51.50%), Query Frame = 2
Query:  122 SLWIGDLQPWMEESYIASIFGHTGEFVSGKVIRNKQTNFSEGYGFIEFVSHAAAERVLQTFNGTQMPSTEQNFRLNWATYGAGERRQDDGPDFTIFVGDLAADVTDYVLQETFRAVYSSVKGAKVVTDRTTGRSKGYGFVRFGDESEQLRSMTEMNGVLCSTRPMRI 622
            ++++G L   + E  +  +F   G  V+  + +++ T   +GYGF+EF+S   A+  ++  N  ++    +  R+N A+  A  +  D G +  IF+G+L  ++ + +L +TF A    ++  K++ D  TG SKGY F+ F        ++  MNG     RP+ +
Sbjct:   14 TVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYG--KPIRVNKAS--AHNKNLDVGAN--IFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITV 174          
The following BLAST results are available for this feature:
BLAST of CN185528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 107
Match NameE-valueIdentityDescription
PABP_COCIM7.250e-1327.38Polyadenylate-binding protein, cytoplasmic and nuc... [more]
PABP_ASPCL7.250e-1327.38Polyadenylate-binding protein, cytoplasmic and nuc... [more]
PABP_USTMA9.468e-1328.57Polyadenylate-binding protein, cytoplasmic and nuc... [more]
ELAV1_MOUSE1.237e-1228.40ELAV-like protein 1 OS=Mus musculus GN=Elavl1 PE=1... [more]
ROC3_ARATH1.615e-1229.1231 kDa ribonucleoprotein, chloroplastic OS=Arabido... [more]
PABP_CRYNE1.615e-1227.38Polyadenylate-binding protein, cytoplasmic and nuc... [more]
PABP_CHAGB1.615e-1226.19Polyadenylate-binding protein, cytoplasmic and nuc... [more]
SF3B4_RAT2.109e-1226.95Splicing factor 3B subunit 4 OS=Rattus norvegicus ... [more]
SF3B4_MOUSE2.109e-1226.95Splicing factor 3B subunit 4 OS=Mus musculus GN=Sf... [more]
SF3B4_HUMAN2.109e-1226.95Splicing factor 3B subunit 4 OS=Homo sapiens GN=SF... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001H18_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001H18, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185528 ID=CN185528; Name=CN185528; organism=Citrus sinensis; type=EST; length=625bp
GCAACAACATCAAACTCCGGTGCCACCTCCGGTTGGATGGACTCCGCAGC
CGGTGCCTCCTCCATCTCAGCAGACGCAGCCCTACGGTGTCGCCCCCGAT
GCTTCCTCCGACGGGATCCGTTCTTTGTGGATCGGAGACTTGCAGCCGTG
GATGGAGGAGAGTTATATCGCCAGCATCTTCGGCCATACTGGAGAGTTTG
TTTCAGGAAAGGTTATTCGCAACAAGCAAACAAATTTTTCAGAGGGTTAT
GGTTTTATTGAGTTTGTTAGTCATGCTGCTGCAGAGAGGGTTTTGCAGAC
TTTTAATGGCACCCAAATGCCAAGCACTGAGCAAAACTTCAGACTGAATT
GGGCAACCTATGGGGCTGGTGAGAGGCGTCAAGATGATGGTCCTGATTTT
ACAATTTTTGTGGGAGATTTGGCTGCCGATGTAACTGACTATGTGCTACA
AGAAACATTTAGAGCTGTCTACTCGTCAGTGAAGGGTGCTAAAGTGGTCA
CTGATAGAACCACTGGACGCTCTAAGGGGTATGGTTTTGTGAGATTTGGG
GATGAAAGTGAACAATTGCGTTCCATGACCGAAATGAATGGCGTGCTCTG
CTCTACTAGACCTATGCGGATTGGG
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