CN185479 | Citrus Genome Database

Overview


Name	CN185479
Unique Name	CN185479
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	577

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Pulp cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CN185479_ssr300	microsatellite	CN185479_ssr300:300..314.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig760	contig	Csv1_Contig760:78..651.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJB4_HUMAN (DnaJ homolog subfamily B member 4 OS=Homo sapiens GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.321e-19
Identity = 45/69 (65.22%), Postives = 56/69 (81.16%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDP---EKFKELAQAYEVLSDPQKREIYDQYGEDALK 290
            YY ILG+ K AS +D+KKAYR+ A++ HPDK   P   EKFKE+A+AYEVLSDP+KREIYDQ+GE+ LK
Sbjct:    5 YYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLK 73

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_SHIFL (Chaperone protein dnaJ OS=Shigella flexneri GN=dnaJ PE=2 SV=3)

HSP 1 Score: 78.9518 bits (193), Expect = 3.972e-19
Identity = 39/71 (54.93%), Postives = 52/71 (73.24%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVSK A + +++KAY+  A++ HPD+  GD E   KFKE+ +AYEVL+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.4242 bits (80), Expect = 3.972e-19
Identity = 16/53 (30.19%), Postives = 28/53 (52.83%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + +E ++E+  +G +K++ +     C  C G G+K G     C  C GSG
Sbjct:  116 DLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCHGSG 168

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_SHIF8 (Chaperone protein dnaJ OS=Shigella flexneri serotype 5b (strain 8401) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.972e-19
Identity = 39/71 (54.93%), Postives = 52/71 (73.24%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVSK A + +++KAY+  A++ HPD+  GD E   KFKE+ +AYEVL+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.4242 bits (80), Expect = 3.972e-19
Identity = 16/53 (30.19%), Postives = 28/53 (52.83%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + +E ++E+  +G +K++ +     C  C G G+K G     C  C GSG
Sbjct:  116 DLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCHGSG 168

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_ECOLI (Chaperone protein dnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3)

HSP 1 Score: 78.9518 bits (193), Expect = 3.972e-19
Identity = 39/71 (54.93%), Postives = 52/71 (73.24%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVSK A + +++KAY+  A++ HPD+  GD E   KFKE+ +AYEVL+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.4242 bits (80), Expect = 3.972e-19
Identity = 16/53 (30.19%), Postives = 28/53 (52.83%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + +E ++E+  +G +K++ +     C  C G G+K G     C  C GSG
Sbjct:  116 DLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCHGSG 168

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_ECODH (Chaperone protein dnaJ OS=Escherichia coli (strain K12 / DH10B) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.972e-19
Identity = 39/71 (54.93%), Postives = 52/71 (73.24%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVSK A + +++KAY+  A++ HPD+  GD E   KFKE+ +AYEVL+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.4242 bits (80), Expect = 3.972e-19
Identity = 16/53 (30.19%), Postives = 28/53 (52.83%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + +E ++E+  +G +K++ +     C  C G G+K G     C  C GSG
Sbjct:  116 DLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCHGSG 168

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_ECOBW (Chaperone protein dnaJ OS=Escherichia coli (strain K12 / BW2952) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.972e-19
Identity = 39/71 (54.93%), Postives = 52/71 (73.24%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVSK A + +++KAY+  A++ HPD+  GD E   KFKE+ +AYEVL+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.4242 bits (80), Expect = 3.972e-19
Identity = 16/53 (30.19%), Postives = 28/53 (52.83%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + +E ++E+  +G +K++ +     C  C G G+K G     C  C GSG
Sbjct:  116 DLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCHGSG 168

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_SERP5 (Chaperone protein dnaJ OS=Serratia proteamaculans (strain 568) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.165e-19
Identity = 35/70 (50.00%), Postives = 51/70 (72.86%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVSK A + ++KKAY+  A++ HPD+  + +   +FKE+ +AYE+L+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSKTAEEREIKKAYKRLAMKFHPDRNQEQDAEARFKEIKEAYEILTDAQKRAAYDQYGHAAFEQ 75

HSP 2 Score: 36.965 bits (84), Expect = 5.165e-19
Identity = 17/53 (32.08%), Postives = 30/53 (56.60%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + +E S+E+  +G +K++ +     C  C G G+K G+S + C  C G G
Sbjct:  115 DLRYNMELSLEEAVRGVTKEIRIPTLEECGVCHGSGAKPGSSPVTCPTCHGQG 167

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_EDWI9 (Chaperone protein dnaJ OS=Edwardsiella ictaluri (strain 93-146) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 6.716e-19
Identity = 38/71 (53.52%), Postives = 52/71 (73.24%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYEILGVS+ A + ++KKAY+  A++ HPD+  GD E   KFKE+ +AYE+L+D QKR  YDQYG  A ++
Sbjct:    6 YYEILGVSREADEREIKKAYKRLAMKYHPDRNQGDKEAEDKFKEIKEAYEILTDAQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.039 bits (79), Expect = 6.716e-19
Identity = 14/53 (26.42%), Postives = 30/53 (56.60%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + ++ ++E+  +G +K++ +     C  C G G+K G+  + C  C G+G
Sbjct:  117 DLRYNMDLTLEEAVRGVTKEIRIPTLAECDVCHGSGAKKGSEPITCPTCHGAG 169

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ_SALAR (Chaperone protein dnaJ OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 6.717e-19
Identity = 39/71 (54.93%), Postives = 51/71 (71.83%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKG-GDPE---KFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            YYE+LGVSK A + ++KKAY+  A++ HPD+  GD E   KFKE+  AYEVL+D QKR  YDQYG  A ++
Sbjct:    6 YYEVLGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAEAKFKEIKAAYEVLTDAQKRAAYDQYGHAAFEQ 76

HSP 2 Score: 35.039 bits (79), Expect = 6.717e-19
Identity = 15/53 (28.30%), Postives = 29/53 (54.72%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGAS-MKCSGCQGSG 564
            D+ + ++ ++E+  +G +K++ +     C  C G G+K+G     C  C GSG
Sbjct:  115 DLRYNMDLTLEEAVRGVTKEIRIPTLEECDVCHGSGAKAGTQPQTCPTCHGSG 167

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJB4_BOVIN (DnaJ homolog subfamily B member 4 OS=Bos taurus GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 7.399e-19
Identity = 44/69 (63.77%), Postives = 56/69 (81.16%), Query Frame = 3

Query:   93 YYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDP---EKFKELAQAYEVLSDPQKREIYDQYGEDALK 290
            YY ILG+ K AS +D+KKAYR+ A++ HPDK   P   E+FKE+A+AYEVLSDP+KREIYDQ+GE+ LK
Sbjct:    5 YYCILGIEKGASDEDIKKAYRKQALRFHPDKNKSPQAEERFKEVAEAYEVLSDPKKREIYDQFGEEGLK 73

The following BLAST results are available for this feature:

BLAST of CN185479 vs. ExPASy Swiss-Prot

BLAST of CN185479 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNJB4_HUMAN	3.321e-19	65.22	DnaJ homolog subfamily B member 4 OS=Homo sapiens ...	[more]
DNAJ_SHIFL	3.972e-19	54.93	Chaperone protein dnaJ OS=Shigella flexneri GN=dna...	[more]
DNAJ_SHIF8	3.972e-19	54.93	Chaperone protein dnaJ OS=Shigella flexneri seroty...	[more]
DNAJ_ECOLI	3.972e-19	54.93	Chaperone protein dnaJ OS=Escherichia coli (strain...	[more]
DNAJ_ECODH	3.972e-19	54.93	Chaperone protein dnaJ OS=Escherichia coli (strain...	[more]
DNAJ_ECOBW	3.972e-19	54.93	Chaperone protein dnaJ OS=Escherichia coli (strain...	[more]
DNAJ_SERP5	5.165e-19	50.00	Chaperone protein dnaJ OS=Serratia proteamaculans ...	[more]
DNAJ_EDWI9	6.716e-19	53.52	Chaperone protein dnaJ OS=Edwardsiella ictaluri (s...	[more]
DNAJ_SALAR	6.717e-19	54.93	Chaperone protein dnaJ OS=Salmonella arizonae (str...	[more]
DNJB4_BOVIN	7.399e-19	63.77	DnaJ homolog subfamily B member 4 OS=Bos taurus GN...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS05_0001G11_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001G11, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN185479 ID=CN185479; Name=CN185479; organism=Citrus sinensis; type=EST; length=577bp
AGTGCCGTCTCGCGTCTACTCTGAAGATCTGAAATTTTTCTAGATACAAG
AAGATGTTCGGAAGAGCGCCCAAGAAAAGCGATAACCCAAAGTACTATGA
GATTCTCGGAGTCTCGAAAAACGCTTCGCAAGATGATCTGAAGAAGGCTT
ATAGGGAAGCCGCCATCCAAAACCATCCTGACAAGGGCGGTGATCCTGAG
AAGTTTAAAGAGTTGGCGCAAGCGTATGAGGTTCTGAGTGACCCCCAGAA
GCGTGAGATATATGATCAATACGGCGAGGATGCGCTTAAAGAGGGAATGG
GTGGTGGTGGTGGTGCCCATGACCCATTCGACATATTCCAATCCTTCTTC
CGTGGCAGCCCATTTGGGGGTGGGAGCAGCAGAGGAGAGAAGGCGAAGGA
GGGGAGAGGACGTACTCCATCCCCTCGAGGGTTCGGTGGAGGATCTGTAC
AAGGGGACATCTAAGAAGCTCTCGCTCTCCCGCAATGGAGTCTGCACCAA
GTGCAGAGGGAAGGGGTCTAAGTCAGGGGCATCGATGAAATGTTCTGGGT
GCCAGGGATCTGGAATGACAGTTTCAA