CN185254

Overview
NameCN185254
Unique NameCN185254
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length683
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2D3_HUMAN (Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens GN=UBE2D3 PE=1 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 8.494e-45
Identity = 81/101 (80.20%), Postives = 90/101 (89.11%), Query Frame = -3 
Query:  376 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 678
            GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   47 GGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2D2_PIG (Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 8.494e-45
Identity = 81/101 (80.20%), Postives = 90/101 (89.11%), Query Frame = -3 
Query:  376 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 678
            GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   47 GGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 5.506e-44
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = -3 
Query:  376 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 678
            GGVF +TIHFP DYPFKPPKVAF T+++HPNINSN SICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   47 GGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 5.153e-42
Identity = 77/101 (76.24%), Postives = 87/101 (86.14%), Query Frame = -3 
Query:  376 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 678
            GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ KY  TAR WTQKYAM
Sbjct:   47 GGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 5.153e-42
Identity = 77/101 (76.24%), Postives = 87/101 (86.14%), Query Frame = -3 
Query:  376 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 678
            GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YK+D+ KY  TAR WTQKYAM
Sbjct:   47 GGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.968e-35
Identity = 68/100 (68.00%), Postives = 81/100 (81.00%), Query Frame = -3 
Query:  379 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 678
            GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  101 GGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.968e-35
Identity = 68/100 (68.00%), Postives = 81/100 (81.00%), Query Frame = -3 
Query:  379 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 678
            GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  101 GGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.775e-34
Identity = 67/100 (67.00%), Postives = 80/100 (80.00%), Query Frame = -3 
Query:  379 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 678
            GGVF + IHF P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y  ++ +++  AR WT++YA
Sbjct:  132 GGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLQNREEHDRIARLWTKRYA 231
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.318e-34
Identity = 67/100 (67.00%), Postives = 81/100 (81.00%), Query Frame = -3 
Query:  379 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 678
            GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   93 GGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192
[Close]
BLAST of CN185254 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.318e-34
Identity = 67/100 (67.00%), Postives = 81/100 (81.00%), Query Frame = -3 
Query:  379 GGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 678
            GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:   93 GGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192
The following BLAST results are available for this feature:
BLAST of CN185254 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 235
Match NameE-valueIdentityDescription
UB2D3_HUMAN8.494e-4580.20Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens... [more]
UB2D2_PIG8.494e-4580.20Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa G... [more]
UB2D3_BOVIN5.506e-4479.21Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G... [more]
U2D2B_RAT5.153e-4276.24Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv... [more]
U2D2B_MOUSE5.153e-4276.24Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu... [more]
UB2E2_MOUSE7.968e-3568.00Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN7.968e-3568.00Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UBCD2_DROME1.775e-3467.00Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi... [more]
UB2E1_MOUSE2.318e-3467.00Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN2.318e-3467.00Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001A21_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001A21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185254 ID=CN185254; Name=CN185254; organism=Citrus sinensis; type=EST; length=683bp
TTTTTTTCCCTTTTACATGATTCGCCTATTTTATCACAAGTTTTCTATTA
TGGAAGAAATTTCCAAGGAAGGAAGCGAAGGCGTTTGTTTCTTCATGATT
ATTGCATGAACACAGACGCGCGCGATGTAAGAATGTTTGGTGCAAAATAG
TAAACAAGTACTTAAGACCATTAACTACCTTCCATCGAACAAGCTTCAAA
CACAAACATTCAACATTAACATAAAAACGTCCCGCCCTTGCAACTCCTCC
CAACACCATTCTAAAACACCAACGTCTGCCCTCCCCAAAGCAAGTTAAAG
AAAATAGATAGACAACACACACAACATATTTCAATAAATGCTCCTTTTTC
TGCAATATCCTAACAACCTTCATCCCATGGCATACTTCTGTGTCCAGCTC
CTGGCCGTGGATTCATATTTGGCCTTGTCGGACTTGTACATGTGGGCAAT
CTCCGGTACAAGAGGGTCGTCGGGATTTGGATCAGTAAGCAAGGAGCAGA
TAGAGAGCAGGACCTTTGAAATGGTAAGCGCTGGACTCCACTGTTCTTTA
AGGATATCAAGACAGATGCTTCCATTGCTGTTGATGTTCGGGTGGAAGAC
CTTGGTCCTAAAAGCAACCTTTGGGGGCTTGAAAGGATAATCAGGAGGAA
AATGAATGGTAACTAAAAATACACCTCCTGCAT
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