CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2462 contig Csv1_Contig2462:1..756. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNS3 (Tryptophan synthase beta chain OS=Synechococcus sp. (strain CC9311) GN=trpB PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.099e-50
Identity = 84/139 (60.43%), Postives = 107/139 (76.98%), Query Frame = -1 
Query:  235 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYRYNQ 651
            +EAAG G+ +G+HAAT+ +G VGV HGAMS LLQD++GQ+   HS+  GL+YPGVGPE S+LRD GRAE+   TD EA+ A + +  LEGI PALE +HA A+LE LCPTLPNG+++V+NCSG GDKDV+TV     NQ
Sbjct:  279 VEAAGDGVATGRHAATITEGRVGVLHGAMSLLLQDQDGQVQEAHSISAGLDYPGVGPEHSYLRDIGRAEYGAVTDDEAITALRLVSELEGIIPALETAHAFAWLEMLCPTLPNGSEIVINCSGRGDKDVNTVAEKLGNQ 417

HSP 2 Score: 33.8834 bits (76), Expect = 2.099e-50
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ D  VRLIGV
Sbjct:  260 NAMGLFHPFVQDTSVRLIGV 279

HSP 3 Score: 28.4906 bits (62), Expect = 2.099e-50
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVLL CVG GS  +G
Sbjct:  245 GRLPDVLLACVGGGSNAMG 263
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROMA (Tryptophan synthase beta chain OS=Prochlorococcus marinus GN=trpB PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 1.325e-49
Identity = 82/132 (62.12%), Postives = 103/132 (78.03%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+ +G+HAAT+ +G +GV HGAMS LLQD  GQ+   HS+  GL+YPGVGPE S+L++ GRAE+   TDQEA+ A Q L   EGI PALE +HALAFLEKLCPTL +G ++V+NCSG GDKDV+TV
Sbjct:  277 VEAAGDGVHTGRHAATITEGRIGVLHGAMSLLLQDSNGQVQEAHSISAGLDYPGVGPEHSYLKEIGRAEYAAVTDQEALSALQLLSEKEGIIPALETAHALAFLEKLCPTLSSGKEIVINCSGRGDKDVNTV 408

HSP 2 Score: 33.8834 bits (76), Expect = 1.325e-49
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH FI D  VR+IGV
Sbjct:  258 NAMGLFHPFIEDRSVRMIGV 277

HSP 3 Score: 29.261 bits (64), Expect = 1.325e-49
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVLL CVG GS  +G
Sbjct:  243 GRSPDVLLACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROM1 (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain NATL1A) GN=trpB PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 2.242e-49
Identity = 79/132 (59.85%), Postives = 107/132 (81.06%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++G+HAAT+ +G VGV HGAMS LLQD++GQ+   HS+  GL+YPGVGPE S+L++ GRAE+   TD EA++A Q + +LEGI PALE +HA A+LEKLCPTL + +++V+NCSG GDKDV+TV
Sbjct:  278 VEAAGDGVETGRHAATITEGRVGVLHGAMSLLLQDKDGQVEEAHSISAGLDYPGVGPEHSYLKEIGRAEYAAVTDTEAIEALQLVSKLEGIIPALETAHAFAYLEKLCPTLNHNSEIVINCSGRGDKDVNTV 409

HSP 2 Score: 34.2686 bits (77), Expect = 2.242e-49
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ D+ VR+IGV
Sbjct:  259 NAMGLFHSFVEDKSVRMIGV 278

HSP 3 Score: 29.261 bits (64), Expect = 2.242e-49
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVLL CVG GS  +G
Sbjct:  244 GRSPDVLLACVGGGSNAMG 262
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROMT (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain NATL2A) GN=trpB PE=3 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 2.917e-49
Identity = 78/132 (59.09%), Postives = 107/132 (81.06%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++G+HAAT+ +G +GV HGAMS LLQD++GQ+   HS+  GL+YPGVGPE S+L++ GRAE+   TD EA++A Q + +LEGI PALE +HA A+LEKLCPTL + +++V+NCSG GDKDV+TV
Sbjct:  278 VEAAGDGVETGRHAATITEGRIGVLHGAMSLLLQDKDGQVEEAHSISAGLDYPGVGPEHSYLKEIGRAEYAAVTDTEAIEALQLVSKLEGIIPALETAHAFAYLEKLCPTLNHNSEIVINCSGRGDKDVNTV 409

HSP 2 Score: 34.2686 bits (77), Expect = 2.917e-49
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ D+ VR+IGV
Sbjct:  259 NAMGLFHSFVEDKSVRMIGV 278

HSP 3 Score: 29.261 bits (64), Expect = 2.917e-49
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVLL CVG GS  +G
Sbjct:  244 GRSPDVLLACVGGGSNAMG 262
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_TRIEI (Tryptophan synthase beta chain OS=Trichodesmium erythraeum (strain IMS101) GN=trpB PE=3 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 8.487e-49
Identity = 91/146 (62.33%), Postives = 112/146 (76.71%), Query Frame = -1 
Query:  256 FMNS*MMKM*D*LELEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 693
            F+N   ++M   + +EAAG G+D+ KHAATL  G VGV HGAMSYLLQDEEGQI+  HS+  GL+YPGVGPE SFL+D+GR E+Y+ TD EAV A+QRL +LEGI PALE +HA+A+LE LCP L    ++V NCSG GDKDV TV
Sbjct:  261 FINDPSVRM---IGVEAAGKGVDTNKHAATLTLGRVGVLHGAMSYLLQDEEGQIIEPHSISAGLDYPGVGPEHSFLKDSGRVEYYSVTDNEAVAAFQRLSQLEGIIPALETAHAIAYLETLCPQLSGSPRIVFNCSGRGDKDVQTV 403

HSP 2 Score: 28.1054 bits (61), Expect = 8.487e-49
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = -2 
Query:  699 GKPDVLLLCVGSGSKCVG 752
            G PD+L+ CVG GS  +G
Sbjct:  239 GLPDILMACVGGGSNAMG 256
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNSC (Tryptophan synthase beta chain OS=Synechococcus sp. (strain CC9605) GN=trpB PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 5.269e-48
Identity = 80/132 (60.61%), Postives = 104/132 (78.79%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+ SG+HAAT+ +G  GV HGAMS LLQD +GQ++  HS+  GL+YPGVGPE S+LR+ GRAE+   TDQ+A+ A + +  LEGI PALE +HA A+LE+LCPTL +G +VV+NCSG GDKDV+TV
Sbjct:  279 VEAAGDGVASGRHAATITEGRAGVLHGAMSLLLQDGDGQVMEAHSISAGLDYPGVGPEHSYLREIGRAEYAAVTDQQALDALRLVSELEGIIPALETAHAFAWLEQLCPTLADGTEVVINCSGRGDKDVNTV 410

HSP 2 Score: 33.4982 bits (75), Expect = 5.269e-48
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ D  VRLIGV
Sbjct:  260 NAMGLFHPFVQDMSVRLIGV 279

HSP 3 Score: 27.7202 bits (60), Expect = 5.269e-48
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVL+ CVG GS  +G
Sbjct:  245 GRLPDVLMACVGGGSNAMG 263
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNPW (Tryptophan synthase beta chain OS=Synechococcus sp. (strain WH7803) GN=trpB PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 6.855e-48
Identity = 82/132 (62.12%), Postives = 104/132 (78.79%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+ +G+HAAT+ +G VGV HGAMS LLQD++GQ+   HS+  GL+YPGVGPE S+LR+ GRAE+   TD EA++A Q + RLEGI PALE +HA A+LE LCPTL  G +VV+NCSG GDKDV+TV
Sbjct:  277 VEAAGDGVATGRHAATITEGRVGVLHGAMSLLLQDQDGQVQEAHSISAGLDYPGVGPEHSYLREIGRAEYGAVTDAEALEALQLVSRLEGIIPALETAHAFAWLETLCPTLSAGTEVVLNCSGRGDKDVNTV 408

HSP 2 Score: 32.7278 bits (73), Expect = 6.855e-48
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+   DVRLIGV
Sbjct:  258 NAMGLFHPFVECTDVRLIGV 277

HSP 3 Score: 27.7202 bits (60), Expect = 6.855e-48
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVL+ CVG GS  +G
Sbjct:  243 GRLPDVLMACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROM9 (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain MIT 9312) GN=trpB PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 6.855e-48
Identity = 80/134 (59.70%), Postives = 102/134 (76.12%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+D+ KHAAT+ KG VG+ HG+MS LLQD+ GQ+   HS+  GL+YPGVGPE S L+D GRAE+ + TDQEA+ A + +  LEGI PALE SHA A+L+KLCPTL     +V+NCSG GDKDV+TV +
Sbjct:  277 VEAAGSGVDTDKHAATITKGSVGILHGSMSLLLQDDNGQVQEAHSISAGLDYPGVGPEHSHLKDIGRAEYGSVTDQEALDALRLVSELEGIIPALETSHAFAWLDKLCPTLEKDTHIVINCSGRGDKDVNTVAS 410

HSP 2 Score: 32.7278 bits (73), Expect = 6.855e-48
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH FI +  VRLIGV
Sbjct:  258 NAMGLFHPFIKETSVRLIGV 277

HSP 3 Score: 28.8758 bits (63), Expect = 6.855e-48
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+LL CVG GS  +G
Sbjct:  243 GSLPDILLACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROMM (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain MIT 9313) GN=trpB PE=3 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 1.159e-47
Identity = 81/139 (58.27%), Postives = 105/139 (75.54%), Query Frame = -1 
Query:  235 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYRYNQ 651
            +EAAG G+++G+HAAT+ +G VGV HGAMS LLQD+EGQ+   HS+  GL+YPGVGPE S+L + GRAE+   +DQ+A+ A + +  LEGI PALE +HA A+LE LCPTL  G +VV+NCSG GDKDV+TV     NQ
Sbjct:  297 VEAAGDGVETGRHAATITEGRVGVLHGAMSLLLQDDEGQVQEAHSISAGLDYPGVGPEHSYLCEIGRAEYAAVSDQQALDALRLVSELEGIIPALETAHAFAWLETLCPTLAAGTEVVINCSGRGDKDVNTVAEKLGNQ 435

HSP 2 Score: 33.4982 bits (75), Expect = 1.159e-47
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ D  VR+IGV
Sbjct:  278 NAMGLFHPFVQDRSVRMIGV 297

HSP 3 Score: 28.4906 bits (62), Expect = 1.159e-47
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVLL CVG GS  +G
Sbjct:  263 GRLPDVLLACVGGGSNAMG 281
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROMS (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain AS9601) GN=trpB PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 1.509e-47
Identity = 79/134 (58.96%), Postives = 102/134 (76.12%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+D+ KHAAT+ KG VG+ HG+MS LLQD+ GQ+   HS+  GL+YPGVGPE S L+D GRAE+ + TDQEA+ A + +  LEGI PALE +HA A+L+KLCPTL     +V+NCSG GDKDV+TV +
Sbjct:  277 VEAAGSGVDTDKHAATITKGSVGILHGSMSLLLQDDNGQVQEAHSISAGLDYPGVGPEHSHLKDIGRAEYGSVTDQEALDALKLVSELEGIIPALETAHAFAWLDKLCPTLEKDTNIVINCSGRGDKDVNTVAS 410

HSP 2 Score: 32.3426 bits (72), Expect = 1.509e-47
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ +  VRLIGV
Sbjct:  258 NAMGLFHPFVKETSVRLIGV 277

HSP 3 Score: 28.8758 bits (63), Expect = 1.509e-47
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+LL CVG GS  +G
Sbjct:  243 GSLPDILLACVGGGSNAMG 261
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_SYNS32.099e-5060.43Tryptophan synthase beta chain OS=Synechococcus sp... [more]
TRPB_PROMA1.325e-4962.12Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_PROM12.242e-4959.85Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_PROMT2.917e-4959.09Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_TRIEI8.487e-4962.33Tryptophan synthase beta chain OS=Trichodesmium er... [more]
TRPB_SYNSC5.269e-4860.61Tryptophan synthase beta chain OS=Synechococcus sp... [more]
TRPB_SYNPW6.855e-4862.12Tryptophan synthase beta chain OS=Synechococcus sp... [more]
TRPB_PROM96.855e-4859.70Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_PROMM1.159e-4758.27Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_PROMS1.509e-4758.96Tryptophan synthase beta chain OS=Prochlorococcus ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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