CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_XANCB (Tryptophan synthase beta chain OS=Xanthomonas campestris pv. campestris (strain B100) GN=trpB PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.258e-36
Identity = 70/131 (53.44%), Postives = 91/131 (69.47%), Query Frame = -1 
Query:  256 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 648
            EAAG G+ SG+HAA++A G  GV HG  +Y++ D++GQI+ THSV  GL+YPGVGPE +FL D+GRA +   TD EA+ A+  L   EGI  ALE+SHA+A   KL   +P  A V+ N SG GDKDV T+
Sbjct:  267 EAAGDGIASGRHAASIAAGRPGVLHGNRTYVICDDDGQIIETHSVSAGLDYPGVGPEHAFLSDSGRAVYQGITDDEALAAFHLLAHTEGILAALESSHAVAQSIKLAREMPKDALVLCNLSGRGDKDVHTI 397

HSP 2 Score: 30.0314 bits (66), Expect = 2.258e-36
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+GLFH F+ND  V++ G
Sbjct:  247 NAIGLFHAFLNDPGVKIYG 265

HSP 3 Score: 24.6386 bits (52), Expect = 2.258e-36
Identity = 9/19 (47.37%), Postives = 11/19 (57.89%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD +  CVG GS  +G
Sbjct:  232 GRLPDAISACVGGGSNAIG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_XANC8 (Tryptophan synthase beta chain OS=Xanthomonas campestris pv. campestris (strain 8004) GN=trpB PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.258e-36
Identity = 70/131 (53.44%), Postives = 91/131 (69.47%), Query Frame = -1 
Query:  256 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 648
            EAAG G+ SG+HAA++A G  GV HG  +Y++ D++GQI+ THSV  GL+YPGVGPE +FL D+GRA +   TD EA+ A+  L   EGI  ALE+SHA+A   KL   +P  A V+ N SG GDKDV T+
Sbjct:  267 EAAGDGIASGRHAASIAAGRPGVLHGNRTYVICDDDGQIIETHSVSAGLDYPGVGPEHAFLSDSGRAVYQGITDDEALAAFHLLAHTEGILAALESSHAVAQSIKLAREMPKDALVLCNLSGRGDKDVHTI 397

HSP 2 Score: 30.0314 bits (66), Expect = 2.258e-36
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+GLFH F+ND  V++ G
Sbjct:  247 NAIGLFHAFLNDPGVKIYG 265

HSP 3 Score: 24.6386 bits (52), Expect = 2.258e-36
Identity = 9/19 (47.37%), Postives = 11/19 (57.89%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD +  CVG GS  +G
Sbjct:  232 GRLPDAISACVGGGSNAIG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_RHIME (Tryptophan synthase beta chain OS=Rhizobium meliloti GN=trpB PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.935e-36
Identity = 68/132 (51.52%), Postives = 88/132 (66.67%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GLD  +H A+L  G  GV HG  +YLLQD +GQI   HS+  GL+YPG+GPE ++L D GR E+    D EA++A+Q L RLEGI PALE SHALA + K  P +     +++N SG GDKD+ TV
Sbjct:  267 VEAGGKGLDGDEHCASLTAGSPGVLHGNRTYLLQDGDGQIKEGHSISAGLDYPGIGPEHAWLNDIGRVEYVPIMDHEALEAFQTLTRLEGIIPALEPSHALAEVIKRAPKMGKDEIILMNLSGRGDKDIFTV 398

HSP 2 Score: 34.2686 bits (77), Expect = 2.935e-36
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH F++DE VR++GV
Sbjct:  248 NAIGIFHSFLDDEGVRIVGV 267

HSP 3 Score: 22.3274 bits (46), Expect = 2.935e-36
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+++  VG GS  +G
Sbjct:  233 GRLPDLVVAAVGGGSNAIG 251
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_XANC5 (Tryptophan synthase beta chain OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=trpB PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 3.814e-36
Identity = 70/131 (53.44%), Postives = 90/131 (68.70%), Query Frame = -1 
Query:  256 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 648
            EAAG G+ +G+HAA++A G  GV HG  +Y++ D++GQI  THS+  GL+YPGVGPE SFL D+GRA +   TD EA+ A+  L   EGI  ALE+SHA+A   KL   LP  A V+ N SG GDKDV T+
Sbjct:  267 EAAGDGIATGRHAASIAAGRPGVLHGNRTYVICDDDGQITETHSISAGLDYPGVGPEHSFLSDSGRAVYQGITDDEAMAAFHLLAHTEGILAALESSHAVAQSIKLARELPRDALVLCNLSGRGDKDVHTI 397

HSP 2 Score: 30.0314 bits (66), Expect = 3.814e-36
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+GLFH F+ND  V++ G
Sbjct:  247 NAIGLFHAFLNDPGVKIYG 265

HSP 3 Score: 24.6386 bits (52), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 11/19 (57.89%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD +  CVG GS  +G
Sbjct:  232 GRLPDAISACVGGGSNAIG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_XANAC (Tryptophan synthase beta chain OS=Xanthomonas axonopodis pv. citri GN=trpB PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 3.814e-36
Identity = 70/131 (53.44%), Postives = 90/131 (68.70%), Query Frame = -1 
Query:  256 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 648
            EAAG G+ +G+HAA++A G  GV HG  +Y++ D++GQI  THS+  GL+YPGVGPE SFL D+GRA +   TD EA+ A+  L   EGI  ALE+SHA+A   KL   LP  A V+ N SG GDKDV T+
Sbjct:  267 EAAGDGIATGRHAASIAAGRPGVLHGNRTYVICDDDGQITETHSISAGLDYPGVGPEHSFLSDSGRAVYQGITDDEAMAAFHLLAHTEGILAALESSHAVAQSIKLARELPRDALVLCNLSGRGDKDVHTI 397

HSP 2 Score: 30.0314 bits (66), Expect = 3.814e-36
Identity = 11/19 (57.89%), Postives = 15/19 (78.95%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+GLFH F+ND  V++ G
Sbjct:  247 NAIGLFHAFLNDPGVKIYG 265

HSP 3 Score: 24.6386 bits (52), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 11/19 (57.89%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD +  CVG GS  +G
Sbjct:  232 GRLPDAISACVGGGSNAIG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BRUSU (Tryptophan synthase beta chain OS=Brucella suis GN=trpB PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.814e-36
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL+  +H A+++ G  GV HG  +YLLQ+ +GQIL  HSV  GL+YPGVGPE S+L+D+GR ++    D EA+ A+Q   R EGI PALE++HA+A   K+ PT+     ++VN SG GDKDV TV
Sbjct:  267 VEAGGRGLEGEEHCASMSAGRPGVLHGNRTYLLQNADGQILEGHSVSAGLDYPGVGPEHSWLKDSGRVDYVPILDNEALDAFQLCTRTEGIIPALESAHAIAQAVKMAPTMGKDKVMIVNLSGRGDKDVHTV 398

HSP 2 Score: 31.5722 bits (70), Expect = 3.814e-36
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F++D  V+++GV
Sbjct:  248 NAIGLFHPFLDDASVKIVGV 267

HSP 3 Score: 23.8682 bits (50), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV++  VG GS  +G
Sbjct:  233 GRLPDVIVAAVGGGSNAIG 251
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BRUME (Tryptophan synthase beta chain OS=Brucella melitensis GN=trpB PE=3 SV=2)

HSP 1 Score: 137.887 bits (346), Expect = 3.814e-36
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL+  +H A+++ G  GV HG  +YLLQ+ +GQIL  HSV  GL+YPGVGPE S+L+D+GR ++    D EA+ A+Q   R EGI PALE++HA+A   K+ PT+     ++VN SG GDKDV TV
Sbjct:  267 VEAGGRGLEGEEHCASMSAGRPGVLHGNRTYLLQNADGQILEGHSVSAGLDYPGVGPEHSWLKDSGRVDYVPILDNEALDAFQLCTRTEGIIPALESAHAIAQAVKMAPTMGKDKVMIVNLSGRGDKDVHTV 398

HSP 2 Score: 31.5722 bits (70), Expect = 3.814e-36
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F++D  V+++GV
Sbjct:  248 NAIGLFHPFLDDASVKIVGV 267

HSP 3 Score: 23.8682 bits (50), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV++  VG GS  +G
Sbjct:  233 GRLPDVIVAAVGGGSNAIG 251
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BRUMB (Tryptophan synthase beta chain OS=Brucella melitensis biotype 2 (strain ATCC 23457) GN=trpB PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.814e-36
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL+  +H A+++ G  GV HG  +YLLQ+ +GQIL  HSV  GL+YPGVGPE S+L+D+GR ++    D EA+ A+Q   R EGI PALE++HA+A   K+ PT+     ++VN SG GDKDV TV
Sbjct:  267 VEAGGRGLEGEEHCASMSAGRPGVLHGNRTYLLQNADGQILEGHSVSAGLDYPGVGPEHSWLKDSGRVDYVPILDNEALDAFQLCTRTEGIIPALESAHAIAQAVKMAPTMGKDKVMIVNLSGRGDKDVHTV 398

HSP 2 Score: 31.5722 bits (70), Expect = 3.814e-36
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F++D  V+++GV
Sbjct:  248 NAIGLFHPFLDDASVKIVGV 267

HSP 3 Score: 23.8682 bits (50), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV++  VG GS  +G
Sbjct:  233 GRLPDVIVAAVGGGSNAIG 251
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BRUC2 (Tryptophan synthase beta chain OS=Brucella canis (strain ATCC 23365 / NCTC 10854) GN=trpB PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.814e-36
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL+  +H A+++ G  GV HG  +YLLQ+ +GQIL  HSV  GL+YPGVGPE S+L+D+GR ++    D EA+ A+Q   R EGI PALE++HA+A   K+ PT+     ++VN SG GDKDV TV
Sbjct:  267 VEAGGRGLEGEEHCASMSAGRPGVLHGNRTYLLQNADGQILEGHSVSAGLDYPGVGPEHSWLKDSGRVDYVPILDNEALDAFQLCTRTEGIIPALESAHAIAQAVKMAPTMGKDKVMIVNLSGRGDKDVHTV 398

HSP 2 Score: 31.5722 bits (70), Expect = 3.814e-36
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F++D  V+++GV
Sbjct:  248 NAIGLFHPFLDDASVKIVGV 267

HSP 3 Score: 23.8682 bits (50), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV++  VG GS  +G
Sbjct:  233 GRLPDVIVAAVGGGSNAIG 251
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BRUAB (Tryptophan synthase beta chain OS=Brucella abortus GN=trpB PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.814e-36
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL+  +H A+++ G  GV HG  +YLLQ+ +GQIL  HSV  GL+YPGVGPE S+L+D+GR ++    D EA+ A+Q   R EGI PALE++HA+A   K+ PT+     ++VN SG GDKDV TV
Sbjct:  267 VEAGGRGLEGEEHCASMSAGRPGVLHGNRTYLLQNADGQILEGHSVSAGLDYPGVGPEHSWLKDSGRVDYVPILDNEALDAFQLCTRTEGIIPALESAHAIAQAVKMAPTMGKDKVMIVNLSGRGDKDVHTV 398

HSP 2 Score: 31.5722 bits (70), Expect = 3.814e-36
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F++D  V+++GV
Sbjct:  248 NAIGLFHPFLDDASVKIVGV 267

HSP 3 Score: 23.8682 bits (50), Expect = 3.814e-36
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV++  VG GS  +G
Sbjct:  233 GRLPDVIVAAVGGGSNAIG 251
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_XANCB2.258e-3653.44Tryptophan synthase beta chain OS=Xanthomonas camp... [more]
TRPB_XANC82.258e-3653.44Tryptophan synthase beta chain OS=Xanthomonas camp... [more]
TRPB_RHIME2.935e-3651.52Tryptophan synthase beta chain OS=Rhizobium melilo... [more]
TRPB_XANC53.814e-3653.44Tryptophan synthase beta chain OS=Xanthomonas camp... [more]
TRPB_XANAC3.814e-3653.44Tryptophan synthase beta chain OS=Xanthomonas axon... [more]
TRPB_BRUSU3.814e-3650.76Tryptophan synthase beta chain OS=Brucella suis GN... [more]
TRPB_BRUME3.814e-3650.76Tryptophan synthase beta chain OS=Brucella meliten... [more]
TRPB_BRUMB3.814e-3650.76Tryptophan synthase beta chain OS=Brucella meliten... [more]
TRPB_BRUC23.814e-3650.76Tryptophan synthase beta chain OS=Brucella canis (... [more]
TRPB_BRUAB3.814e-3650.76Tryptophan synthase beta chain OS=Brucella abortus... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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