CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROMP (Tryptophan synthase beta chain OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) GN=trpB PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 1.963e-47
Identity = 78/134 (58.21%), Postives = 104/134 (77.61%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+++ KHAAT+ KG VG+ HG+MS LLQD++GQ+   HS+  GL+YPGVGPE S+L+D GRAE+ + TD EA+ A + +  LEGI PALE +HA A+LEKLCPTL    ++V+NCSG GDKDV+TV +
Sbjct:  276 VEAAGSGVNTEKHAATITKGSVGILHGSMSLLLQDKDGQVQEAHSISAGLDYPGVGPEHSYLKDIGRAEYGSVTDAEALDALKLVSELEGIIPALETAHAFAWLEKLCPTLDKDTEIVINCSGRGDKDVNTVAS 409

HSP 2 Score: 33.113 bits (74), Expect = 1.963e-47
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ ++ VRLIGV
Sbjct:  257 NAMGLFHPFVKEKSVRLIGV 276

HSP 3 Score: 28.8758 bits (63), Expect = 1.963e-47
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+LL CVG GS  +G
Sbjct:  242 GSLPDILLACVGGGSNAMG 260
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROM5 (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain MIT 9515) GN=trpB PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 1.963e-47
Identity = 78/134 (58.21%), Postives = 103/134 (76.87%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+++ KHAAT+ KG VG+ HG+MS LLQD+ GQ+   HS+  GL+YPGVGPE S+L+D GRAE+ + TD EA+ A + +  LEGI PALE +HA A+LEKLCPTL    ++V+NCSG GDKDV+TV +
Sbjct:  277 VEAAGSGVNTDKHAATITKGSVGILHGSMSLLLQDKNGQVQEAHSISAGLDYPGVGPEHSYLKDIGRAEYGSVTDTEALNALKLVSELEGIIPALETAHAFAWLEKLCPTLEKNTEIVINCSGRGDKDVNTVAS 410

HSP 2 Score: 33.113 bits (74), Expect = 1.963e-47
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ ++ VRLIGV
Sbjct:  258 NAMGLFHPFVKEKSVRLIGV 277

HSP 3 Score: 28.8758 bits (63), Expect = 1.963e-47
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+LL CVG GS  +G
Sbjct:  243 GSLPDILLACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNJB (Tryptophan synthase beta chain OS=Synechococcus sp. GN=trpB PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.552e-47
Identity = 83/143 (58.04%), Postives = 103/143 (72.03%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCP--------TLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG GL + +HAATL +G +GV HGAMSYLLQDEEGQ+   HS+  GL+YPGVGPE S+L++ GRAE+Y  TD EAV+A   L RLEGI PALE +HALA+L+ L P         +    +VVVNCSG GDKD++TV  Y
Sbjct:  275 IEAAGEGLHTPRHAATLNRGRIGVLHGAMSYLLQDEEGQVQEAHSISAGLDYPGVGPEHSYLKEIGRAEYYAVTDDEAVEALLLLSRLEGIIPALETAHALAYLKTLAPQQGLRPANAIGPQQRVVVNCSGRGDKDLNTVFKY 417

HSP 2 Score: 33.8834 bits (76), Expect = 2.552e-47
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NALGLF+EF+ D  VRLIG+
Sbjct:  256 NALGLFNEFVRDPQVRLIGI 275

HSP 3 Score: 31.5722 bits (70), Expect = 2.552e-47
Identity = 13/19 (68.42%), Postives = 14/19 (73.68%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PDVLL CVG GS  +G
Sbjct:  241 GGLPDVLLACVGGGSNALG 259
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNPX (Tryptophan synthase beta chain OS=Synechococcus sp. (strain WH8102) GN=trpB PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 2.553e-47
Identity = 80/132 (60.61%), Postives = 105/132 (79.55%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+ +G+HAAT+ +G  GV HGAMS LLQD +GQ+   HS+  GL+YPGVGPE S+LR+ GRAE+   TDQ+A+ A + +  LEGI PALE +HA A+LE+LCPTLP+G++VV+NCSG GDKDV+TV
Sbjct:  279 VEAAGDGVATGRHAATITEGRAGVLHGAMSLLLQDSDGQVQEAHSISAGLDYPGVGPEHSYLREIGRAEYGAVTDQQALGALRLVSELEGIIPALETAHAFAWLEQLCPTLPDGSEVVINCSGRGDKDVNTV 410

HSP 2 Score: 30.8018 bits (68), Expect = 2.553e-47
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+   +VRLIGV
Sbjct:  260 NAMGLFHPFVECTNVRLIGV 279

HSP 3 Score: 27.7202 bits (60), Expect = 2.553e-47
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVL+ CVG GS  +G
Sbjct:  245 GRLPDVLMACVGGGSNAMG 263
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROM2 (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain MIT 9215) GN=trpB PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 2.553e-47
Identity = 79/134 (58.96%), Postives = 102/134 (76.12%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+D+ KHAAT+ KG VG+ HG+MS LLQD+ GQ+   HS+  GL+YPGVGPE S L+D GRAE+ + TDQEA+ + + +  LEGI PALE SHA A+L+KLCPTL     +V+NCSG GDKDV+TV +
Sbjct:  277 VEAAGGGVDTDKHAATITKGSVGILHGSMSLLLQDDNGQVQEAHSISAGLDYPGVGPEHSHLKDIGRAEYGSVTDQEALDSLRLVSELEGIIPALETSHAFAWLDKLCPTLEKDTHIVINCSGRGDKDVNTVAS 410

HSP 2 Score: 32.3426 bits (72), Expect = 2.553e-47
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ +  VRLIGV
Sbjct:  258 NAMGLFHPFVKETSVRLIGV 277

HSP 3 Score: 28.8758 bits (63), Expect = 2.553e-47
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+LL CVG GS  +G
Sbjct:  243 GSLPDILLACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROM0 (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain MIT 9301) GN=trpB PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.322e-47
Identity = 79/134 (58.96%), Postives = 102/134 (76.12%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+D+ KHAAT+ KG VG+ HG+MS LLQD+ GQ+   HS+  GL+YPGVGPE S L++ GRAE+ + TDQEA+ A + +  LEGI PALE SHA A+L+KLCPTL     +V+NCSG GDKDV+TV +
Sbjct:  277 VEAAGSGVDTDKHAATITKGSVGILHGSMSLLLQDDNGQVQEAHSISAGLDYPGVGPEHSHLKEIGRAEYGSVTDQEALDALKLVSELEGIIPALETSHAFAWLDKLCPTLEKDTHIVINCSGRGDKDVNTVAS 410

HSP 2 Score: 32.3426 bits (72), Expect = 3.322e-47
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ +  VRLIGV
Sbjct:  258 NAMGLFHPFVKETSVRLIGV 277

HSP 3 Score: 28.4906 bits (62), Expect = 3.322e-47
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD+LL CVG GS  +G
Sbjct:  243 GSFPDILLACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNS9 (Tryptophan synthase beta chain OS=Synechococcus sp. (strain CC9902) GN=trpB PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.237e-46
Identity = 76/132 (57.58%), Postives = 102/132 (77.27%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++ +HAAT+ +G  GV HGAMS LLQD +GQ+   HS+  GL+YPGVGPE S+L++ GRAE+   TD++A+   + +  LEGI PALE +HA A+LE+LCPTLP G +VV+NCSG GDKDV+TV
Sbjct:  279 VEAAGDGVNTPRHAATITEGRAGVLHGAMSLLLQDSDGQVQEAHSISAGLDYPGVGPEHSYLKEIGRAEYAAVTDEQALDGLRLVSELEGIIPALETAHAFAWLEQLCPTLPQGCEVVINCSGRGDKDVNTV 410

HSP 2 Score: 33.8834 bits (76), Expect = 1.237e-46
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ D  VRLIGV
Sbjct:  260 NAMGLFHPFVQDTSVRLIGV 279

HSP 3 Score: 27.7202 bits (60), Expect = 1.237e-46
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVL+ CVG GS  +G
Sbjct:  245 GRLPDVLMACVGGGSNAMG 263
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_PROM4 (Tryptophan synthase beta chain OS=Prochlorococcus marinus (strain MIT 9211) GN=trpB PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 1.318e-45
Identity = 75/132 (56.82%), Postives = 102/132 (77.27%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++ +HAAT+ +G VGV HGAMS LLQD +GQ+   HS+  GL+YPGVGPE S+L + GRAE+   TD+EA+ A + + +LEGI PALE +HA A+L+ LCP+L  G ++V+NCSG GDKDV+TV
Sbjct:  277 VEAAGDGVNTKRHAATITQGSVGVLHGAMSLLLQDSDGQVQEAHSISAGLDYPGVGPEHSYLNEIGRAEYVAVTDKEALNALELVSKLEGIIPALETAHAFAWLDTLCPSLAPGTEIVINCSGRGDKDVNTV 408

HSP 2 Score: 31.9574 bits (71), Expect = 1.318e-45
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH FI D  V++IGV
Sbjct:  258 NAMGLFHSFIEDLSVKMIGV 277

HSP 3 Score: 29.261 bits (64), Expect = 1.318e-45
Identity = 12/19 (63.16%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVLL CVG GS  +G
Sbjct:  243 GRSPDVLLACVGGGSNAMG 261
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_METPP (Tryptophan synthase beta chain OS=Methylibium petroleiphilum (strain PM1) GN=trpB PE=3 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 2.230e-45
Identity = 80/134 (59.70%), Postives = 102/134 (76.12%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG GLDSGKHAA+L+ G  GV HG  +YLLQD  GQI+ THS+  GL+YPGVGPE ++L+D GRAE+   TD EA+QA+ RLCR EGI PALE+SHA+A+  KL PT+ +   ++VN SG GDKD+ TV +
Sbjct:  260 VEAAGHGLDSGKHAASLSAGSPGVLHGNRTYLLQDANGQIIETHSISAGLDYPGVGPEHAYLKDIGRAEYVGITDDEALQAFHRLCRTEGIIPALESSHAVAYAMKLAPTMRSDQSLLVNLSGRGDKDIGTVAD 393

HSP 2 Score: 29.261 bits (64), Expect = 2.230e-45
Identity = 12/20 (60.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +I  E VRLIGV
Sbjct:  241 NAMGIFYPYIRHEGVRLIGV 260

HSP 3 Score: 26.1794 bits (56), Expect = 2.230e-45
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  226 GRQPDAVIACVGGGSNAMG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_GLOVI (Tryptophan synthase beta chain OS=Gloeobacter violaceus GN=trpB PE=3 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 1.826e-44
Identity = 77/132 (58.33%), Postives = 97/132 (73.48%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++GKHAATL  G  GV HGAMSY+LQDE+GQ+   HS+  GL+YPGVGPE S+L+D GRAE+Y+ TD EA+ A   +C  EGI PALE +HA A+L  L   L +   VV+NCSG GDKD+ TV
Sbjct:  273 IEAAGEGIETGKHAATLTAGRAGVLHGAMSYVLQDEQGQVQEAHSLSAGLDYPGVGPEHSYLKDIGRAEYYSVTDSEALAALSLVCSTEGIIPALETAHAFAYLGILASQLHSEQIVVLNCSGRGDKDMGTV 404

HSP 2 Score: 32.3426 bits (72), Expect = 1.826e-44
Identity = 11/20 (55.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH+F+++  VRL+G+
Sbjct:  254 NAIGLFHDFLDERAVRLVGI 273

HSP 3 Score: 28.4906 bits (62), Expect = 1.826e-44
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDVL+ CVG GS  +G
Sbjct:  239 GRLPDVLIACVGGGSNAIG 257
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_PROMP1.963e-4758.21Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_PROM51.963e-4758.21Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_SYNJB2.552e-4758.04Tryptophan synthase beta chain OS=Synechococcus sp... [more]
TRPB_SYNPX2.553e-4760.61Tryptophan synthase beta chain OS=Synechococcus sp... [more]
TRPB_PROM22.553e-4758.96Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_PROM03.322e-4758.96Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_SYNS91.237e-4657.58Tryptophan synthase beta chain OS=Synechococcus sp... [more]
TRPB_PROM41.318e-4556.82Tryptophan synthase beta chain OS=Prochlorococcus ... [more]
TRPB_METPP2.230e-4559.70Tryptophan synthase beta chain OS=Methylibium petr... [more]
TRPB_GLOVI1.826e-4458.33Tryptophan synthase beta chain OS=Gloeobacter viol... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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