CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NITEC (Tryptophan synthase beta chain OS=Nitrosomonas eutropha (strain C91) GN=trpB PE=3 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.227e-44
Identity = 78/132 (59.09%), Postives = 97/132 (73.48%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+D+ KHAATL  G  GV HG  +YL+QDE GQI+ THS+  GL+YPGVGPE ++L+D GRAE+   TD EA+ A+  LCR EGI PALE+SHALA+  KL PTL     ++VN SG GDKD+ TV
Sbjct:  260 VEAAGKGIDTQKHAATLVTGRPGVLHGNRTYLIQDENGQIIETHSISAGLDYPGVGPEHAWLKDCGRAEYVAITDGEALAAFHALCRFEGIMPALESSHALAYAAKLAPTLRKDQLLLVNLSGRGDKDMATV 391

HSP 2 Score: 31.187 bits (69), Expect = 5.227e-44
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ +I +E+VR+IGV
Sbjct:  241 NAIGLFYPYIGEENVRMIGV 260

HSP 3 Score: 28.1054 bits (61), Expect = 5.227e-44
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD L+ CVG GS  +G
Sbjct:  226 GRQPDALIACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NITEU (Tryptophan synthase beta chain OS=Nitrosomonas europaea GN=trpB PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 5.228e-44
Identity = 77/132 (58.33%), Postives = 97/132 (73.48%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+D+ +HAATL  G  GV HG  +YL+QDE GQI+ THS+  GL+YPGVGPE ++L+D GRAE+   TD EA+ A+  LCR EGI PALE+SHALA+  KL PTL     ++VN SG GDKD+ TV
Sbjct:  258 VEAAGKGIDTHEHAATLVTGRPGVLHGNRTYLIQDENGQIIETHSISAGLDYPGVGPEHAWLKDCGRAEYVAVTDDEALAAFHALCRFEGIIPALESSHALAYAAKLAPTLNKDQLLLVNLSGRGDKDMATV 389

HSP 2 Score: 31.5722 bits (70), Expect = 5.228e-44
Identity = 12/20 (60.00%), Postives = 19/20 (95.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ +I++E+VR+IGV
Sbjct:  239 NAIGLFYPYIDEENVRMIGV 258

HSP 3 Score: 28.1054 bits (61), Expect = 5.228e-44
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD L+ CVG GS  +G
Sbjct:  224 GRQPDALIACVGGGSNAIG 242
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_RALSO (Tryptophan synthase beta chain OS=Ralstonia solanacearum GN=trpB PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 6.797e-44
Identity = 80/132 (60.61%), Postives = 103/132 (78.03%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG GL+SG+HAA+L  G  GV HG  +YLLQDE+GQI+ THS+  GL+YPGVGPE ++L+D GRA++   TD+EA+QA+  LCR+EGI PALE+SHALA+  KL PTLP    ++VN SG GDKD+ TV
Sbjct:  264 VEAAGEGLESGRHAASLTGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGPEHAWLKDAGRAQYVGITDKEALQAFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTV 395

HSP 2 Score: 26.1794 bits (56), Expect = 6.797e-44
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  230 GRQPDAVIACVGGGSNAMG 248

HSP 3 Score: 26.1794 bits (56), Expect = 6.797e-44
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +I+   V+LIGV
Sbjct:  245 NAMGIFYPYIDHASVQLIGV 264
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_DECAR (Tryptophan synthase beta chain OS=Dechloromonas aromatica (strain RCB) GN=trpB PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 1.495e-43
Identity = 77/132 (58.33%), Postives = 100/132 (75.76%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++G+H+A+L  G  GV HG  +YLLQDE+GQI+ THSV  GL+YPGVGPE ++L+D GRAE+   TD EA+ A+  LCR+EGI PALE+SHALA+  KL PTL     ++VN SG GDKD+ TV
Sbjct:  260 VEAAGDGMETGRHSASLTAGVPGVLHGNRTYLLQDEDGQIIETHSVSAGLDYPGVGPEHAWLKDLGRAEYQPVTDSEALAAFHTLCRIEGIIPALESSHALAYAAKLAPTLAKDKILLVNLSGRGDKDMHTV 391

HSP 2 Score: 30.8018 bits (68), Expect = 1.495e-43
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +IN E VRLIGV
Sbjct:  241 NAMGIFYPYINVEGVRLIGV 260

HSP 3 Score: 26.1794 bits (56), Expect = 1.495e-43
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  226 GRQPDAVIACVGGGSNAMG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_CLOK5 (Tryptophan synthase beta chain OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=trpB PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.945e-43
Identity = 82/132 (62.12%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+D+ KHAA++AKG VGV HG M+YL+QD+EGQIL  +SV  GL+YPGVGPE ++L+DT RAE+   TDQEA+ A+  L R EGI PALE+SHALA+  KL P L     VVVN SG GDKDVDT+
Sbjct:  258 VEAAGLGVDTDKHAASMAKGSVGVLHGMMTYLIQDDEGQILPVYSVSAGLDYPGVGPEHAYLKDTKRAEYTYVTDQEALDAFGYLSRCEGIIPALESSHALAYTMKLAPELSKEEIVVVNISGRGDKDVDTI 389

HSP 2 Score: 29.261 bits (64), Expect = 1.945e-43
Identity = 10/20 (50.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            N++G+F+ F+ D+ V+LIGV
Sbjct:  239 NSMGIFYPFVEDKSVKLIGV 258

HSP 3 Score: 22.7126 bits (47), Expect = 1.945e-43
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  P+ ++ CVG GS  +G
Sbjct:  224 GKLPNYIIACVGGGSNSMG 242
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_CLOK1 (Tryptophan synthase beta chain OS=Clostridium kluyveri (strain NBRC 12016) GN=trpB PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.945e-43
Identity = 82/132 (62.12%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+D+ KHAA++AKG VGV HG M+YL+QD+EGQIL  +SV  GL+YPGVGPE ++L+DT RAE+   TDQEA+ A+  L R EGI PALE+SHALA+  KL P L     VVVN SG GDKDVDT+
Sbjct:  258 VEAAGLGVDTDKHAASMAKGSVGVLHGMMTYLIQDDEGQILPVYSVSAGLDYPGVGPEHAYLKDTKRAEYTYVTDQEALDAFGYLSRCEGIIPALESSHALAYTMKLAPELSKEEIVVVNISGRGDKDVDTI 389

HSP 2 Score: 29.261 bits (64), Expect = 1.945e-43
Identity = 10/20 (50.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            N++G+F+ F+ D+ V+LIGV
Sbjct:  239 NSMGIFYPFVEDKSVKLIGV 258

HSP 3 Score: 22.7126 bits (47), Expect = 1.945e-43
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  P+ ++ CVG GS  +G
Sbjct:  224 GKLPNYIIACVGGGSNSMG 242
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_THETN (Tryptophan synthase beta chain OS=Thermoanaerobacter tengcongensis GN=trpB PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 7.234e-43
Identity = 78/132 (59.09%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++GKHA+ +AKG VGV HG M+YLLQDEEG+IL  HS+  GL+YPGVGPE ++L++ GRAE+  ATD+EA+ A+  L R EGI PALE++HALA+  KL   L     +VVN SG GDKDV+TV
Sbjct:  252 VEAAGEGIETGKHASAMAKGSVGVLHGMMTYLLQDEEGKILPVHSISAGLDYPGVGPEHAYLKEIGRAEYVYATDEEALAAFMDLSRTEGIIPALESAHALAYAMKLAKKLDEDKIIVVNLSGRGDKDVNTV 383

HSP 2 Score: 30.0314 bits (66), Expect = 7.234e-43
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ FI D+ V+L+GV
Sbjct:  233 NAMGIFYPFIEDKGVKLVGV 252

HSP 3 Score: 24.2534 bits (51), Expect = 7.234e-43
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  218 GRLPDYVVACVGGGSNAMG 236
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_THEPX (Tryptophan synthase beta chain OS=Thermoanaerobacter sp. (strain X514) GN=trpB PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 9.407e-43
Identity = 75/132 (56.82%), Postives = 102/132 (77.27%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++GKHAA +AKG VGV HG M+YLLQDEEG+I+  +S+  GL+YPGVGPE +FL+++ RA++  ATD+EA+ A+  L + EGI PALE++HALA+  KL P L     ++VN SG GDKDV+TV
Sbjct:  252 VEAAGEGIETGKHAAAMAKGSVGVLHGMMTYLLQDEEGRIMPVYSISAGLDYPGVGPEHAFLKESNRAQYVYATDEEALAAFMDLSQTEGIIPALESAHALAYAMKLAPNLTKDNIIIVNLSGRGDKDVNTV 383

HSP 2 Score: 31.5722 bits (70), Expect = 9.407e-43
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ FI D++V+LIGV
Sbjct:  233 NAMGIFYPFIEDKEVKLIGV 252

HSP 3 Score: 24.6386 bits (52), Expect = 9.407e-43
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  218 GRLPDYVIACVGGGSNAMG 236
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_THEP3 (Tryptophan synthase beta chain OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=trpB PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 9.407e-43
Identity = 75/132 (56.82%), Postives = 102/132 (77.27%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++GKHAA +AKG VGV HG M+YLLQDEEG+I+  +S+  GL+YPGVGPE +FL+++ RA++  ATD+EA+ A+  L + EGI PALE++HALA+  KL P L     ++VN SG GDKDV+TV
Sbjct:  252 VEAAGEGIETGKHAAAMAKGSVGVLHGMMTYLLQDEEGRIMPVYSISAGLDYPGVGPEHAFLKESNRAQYVYATDEEALAAFMDLSQTEGIIPALESAHALAYAMKLAPNLTKDNIIIVNLSGRGDKDVNTV 383

HSP 2 Score: 31.5722 bits (70), Expect = 9.407e-43
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ FI D++V+LIGV
Sbjct:  233 NAMGIFYPFIEDKEVKLIGV 252

HSP 3 Score: 24.6386 bits (52), Expect = 9.407e-43
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  218 GRLPDYVIACVGGGSNAMG 236
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_RHOFD (Tryptophan synthase beta chain OS=Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) GN=trpB PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 2.683e-42
Identity = 75/134 (55.97%), Postives = 99/134 (73.88%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+DSGKH+A+L +G  GV HG  +Y+LQD+ GQ+  THS+  GL+YPGVGPE +FL+D GRAE+   TDQEA+ A+  LCR EGI PALE+SHA+A+  KL  T+     ++VN SG GDKD+ TV +
Sbjct:  266 VEAAGLGMDSGKHSASLQRGSPGVLHGNRTYVLQDDNGQVTETHSISAGLDYPGVGPEHAFLKDIGRAEYVGITDQEALAAFHYLCRTEGIIPALESSHAVAYAMKLAKTMRPDQSILVNLSGRGDKDIGTVAD 399

HSP 2 Score: 28.8758 bits (63), Expect = 2.683e-42
Identity = 12/20 (60.00%), Postives = 15/20 (75.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH +I  E  RLIGV
Sbjct:  247 NAMGIFHPYIAHEATRLIGV 266

HSP 3 Score: 24.2534 bits (51), Expect = 2.683e-42
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = -2 
Query:  699 KPDVLLLCVGSGSKCVG 749
            +PD ++ CVG GS  +G
Sbjct:  234 QPDAVVACVGGGSNAMG 250
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_NITEC5.227e-4459.09Tryptophan synthase beta chain OS=Nitrosomonas eut... [more]
TRPB_NITEU5.228e-4458.33Tryptophan synthase beta chain OS=Nitrosomonas eur... [more]
TRPB_RALSO6.797e-4460.61Tryptophan synthase beta chain OS=Ralstonia solana... [more]
TRPB_DECAR1.495e-4358.33Tryptophan synthase beta chain OS=Dechloromonas ar... [more]
TRPB_CLOK51.945e-4362.12Tryptophan synthase beta chain OS=Clostridium kluy... [more]
TRPB_CLOK11.945e-4362.12Tryptophan synthase beta chain OS=Clostridium kluy... [more]
TRPB_THETN7.234e-4359.09Tryptophan synthase beta chain OS=Thermoanaerobact... [more]
TRPB_THEPX9.407e-4356.82Tryptophan synthase beta chain OS=Thermoanaerobact... [more]
TRPB_THEP39.407e-4356.82Tryptophan synthase beta chain OS=Thermoanaerobact... [more]
TRPB_RHOFD2.683e-4255.97Tryptophan synthase beta chain OS=Rhodoferax ferri... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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