CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_POLNA (Tryptophan synthase beta chain OS=Polaromonas naphthalenivorans (strain CJ2) GN=trpB PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 5.899e-42
Identity = 73/134 (54.48%), Postives = 101/134 (75.37%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG GLDSGKH+A+L +G  GV HG  +Y+LQ+++GQ+  THS+  GL+YPGVGPE ++L+D GRAE+   TD EA+QA+  LCR EGI PALE++HA+A+  KL  T+ +   ++VN SG GDKD+ TV +
Sbjct:  261 VEAAGEGLDSGKHSASLQRGLPGVLHGNRTYVLQNDDGQVTETHSISAGLDYPGVGPEHAYLKDIGRAEYVGITDTEALQAFHYLCRTEGIIPALESAHAVAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVAD 394

HSP 2 Score: 30.0314 bits (66), Expect = 5.899e-42
Identity = 12/20 (60.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH +I  E+ RLIGV
Sbjct:  242 NAMGIFHPYIAHENTRLIGV 261

HSP 3 Score: 25.7942 bits (55), Expect = 5.899e-42
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  227 GRQPDAVVACVGGGSNAMG 245
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_ACIF5 (Tryptophan synthase beta chain OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=trpB PE=3 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.495e-41
Identity = 76/132 (57.58%), Postives = 100/132 (75.76%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL +G+HAA L  G  GV HG  +YL++DE+GQIL THS+  GL+YPGVGPE ++L+DTGRAE+  ATD+EA+ A+ RLCR EGI PALE++HALA   +L PT+ +   ++VN SG GDKD+ TV
Sbjct:  260 VEAGGLGLGTGQHAAPLTTGRPGVLHGNRTYLMEDEDGQILPTHSISAGLDYPGVGPEHAYLKDTGRAEYVAATDEEALAAFHRLCRTEGIIPALESAHALAHAFRLAPTMRSDQTIIVNLSGRGDKDIHTV 391

HSP 2 Score: 26.5646 bits (57), Expect = 1.495e-41
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD L+ CVG GS  +G
Sbjct:  226 GRLPDCLIACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_ACIF2 (Tryptophan synthase beta chain OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=trpB PE=3 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.495e-41
Identity = 76/132 (57.58%), Postives = 100/132 (75.76%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL +G+HAA L  G  GV HG  +YL++DE+GQIL THS+  GL+YPGVGPE ++L+DTGRAE+  ATD+EA+ A+ RLCR EGI PALE++HALA   +L PT+ +   ++VN SG GDKD+ TV
Sbjct:  260 VEAGGLGLGTGQHAAPLTTGRPGVLHGNRTYLMEDEDGQILPTHSISAGLDYPGVGPEHAYLKDTGRAEYVAATDEEALAAFHRLCRTEGIIPALESAHALAHAFRLAPTMRSDQTIIVNLSGRGDKDIHTV 391

HSP 2 Score: 26.5646 bits (57), Expect = 1.495e-41
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD L+ CVG GS  +G
Sbjct:  226 GRLPDCLIACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LEPCP (Tryptophan synthase beta chain OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=trpB PE=3 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 2.193e-41
Identity = 76/134 (56.72%), Postives = 98/134 (73.13%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G++SGKH+A+L +G  GV HG  +Y+LQD  GQI  THSV  GL+YPGVGPE ++L+D GRAE+   TD EA+QA+  LCR EGI PALE+SHA+A+  KL  T+     V+VN SG GDKD+ TV +
Sbjct:  260 VEAAGEGMESGKHSASLQRGSPGVLHGNRTYVLQDANGQITETHSVSAGLDYPGVGPEHAYLKDIGRAEYVGITDAEALQAFHYLCRAEGIIPALESSHAVAYAMKLAKTMRPDQHVLVNLSGRGDKDIGTVAD 393

HSP 2 Score: 28.4906 bits (62), Expect = 2.193e-41
Identity = 12/20 (60.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +I  E VRLIGV
Sbjct:  241 NAMGIFYPYIPHEGVRLIGV 260

HSP 3 Score: 25.7942 bits (55), Expect = 2.193e-41
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  226 GRQPDAVVACVGGGSNAMG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_ACICJ (Tryptophan synthase beta chain OS=Acidiphilium cryptum (strain JF-5) GN=trpB PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 2.196e-41
Identity = 74/135 (54.81%), Postives = 99/135 (73.33%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG GLDSG+HAA+L++G  GV HG  +YLLQD++GQIL  HS+  GL+YPG+GPE S+L D GR E+ +ATD EA+ A+Q  CR EGI PALE SHALA + K+ P +   + +++N  G GDKD+ TV  +
Sbjct:  265 VEAAGRGLDSGEHAASLSRGRPGVLHGNRTYLLQDDDGQILEAHSISAGLDYPGIGPEHSYLHDIGRVEYVSATDDEALAAFQLCCRTEGIIPALEPSHALAHVAKIAPGMDKESIILMNLCGRGDKDIFTVARH 399

HSP 2 Score: 30.0314 bits (66), Expect = 2.196e-41
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ DE V+LIGV
Sbjct:  247 NAIGLFHAFL-DEQVKLIGV 265

HSP 3 Score: 22.7126 bits (47), Expect = 2.196e-41
Identity = 8/19 (42.11%), Postives = 11/19 (57.89%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV +  +G GS  +G
Sbjct:  232 GRLPDVAIAAIGGGSNAIG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BORPD (Tryptophan synthase beta chain OS=Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448) GN=trpB PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 2.856e-41
Identity = 75/132 (56.82%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG GLDSG+HAA+LA G+VGV HG  +Y++Q+ +GQ+  THSV  GL+YPGVGPE ++L+D+GRA++   TD EA+ A+   CR+EGI PALE+SHA+A   K+ PTLP    ++VN SG GDKD+ TV
Sbjct:  260 VEAAGEGLDSGRHAASLAAGQVGVLHGNRTYVMQNADGQVQETHSVSAGLDYPGVGPEHAWLKDSGRAQYVGITDDEALAAFHDCCRIEGIMPALESSHAIAQAVKMAPTLPKDTIILVNLSGRGDKDMHTV 391

HSP 2 Score: 29.261 bits (64), Expect = 2.856e-41
Identity = 12/20 (60.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +I  E+VRLIGV
Sbjct:  241 NAMGIFYPYIPHENVRLIGV 260

HSP 3 Score: 21.557 bits (44), Expect = 2.856e-41
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++  VG GS  +G
Sbjct:  226 GRQPDYVVASVGGGSNAMG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_METCA (Tryptophan synthase beta chain OS=Methylococcus capsulatus GN=trpB PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 3.712e-41
Identity = 71/132 (53.79%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++G+H+A L+ G  GV HG  +YL++DE+G+I+ THS+  GL+YPGVGPE ++L+D GRA + +ATD EA++A+  L R EGI PALE+SHA+A+  KL PTL +   V+VN SG GDKD+ T+
Sbjct:  266 VEAAGDGIETGRHSAPLSAGRPGVLHGNRTYLMEDEDGEIIETHSISAGLDYPGVGPEHAWLKDCGRASYVSATDAEALEAFHILTRSEGIIPALESSHAVAYALKLAPTLSSDKIVLVNLSGRGDKDIHTI 397

HSP 2 Score: 30.4166 bits (67), Expect = 3.712e-41
Identity = 11/20 (55.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F++D +V + GV
Sbjct:  247 NAIGLFHPFVDDREVAMYGV 266

HSP 3 Score: 26.5646 bits (57), Expect = 3.712e-41
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD L+ CVG GS  +G
Sbjct:  232 GRLPDALVACVGGGSNAIG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_ACIAC (Tryptophan synthase beta chain OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=trpB PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 4.822e-41
Identity = 74/134 (55.22%), Postives = 99/134 (73.88%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG GLDSGKH+A+L +G  GV HG  +++LQDE GQI  THS+  GL+YPGVGPE ++L++ GRAE+   TD+EA+ A+  LCR EGI PALE+SHA+A+  +L  TL     ++VN SG GDKD+ TV +
Sbjct:  266 VEAAGEGLDSGKHSASLQRGSAGVLHGNRTFILQDENGQITETHSISAGLDYPGVGPEHAWLQEIGRAEYVGITDREALDAFHYLCRTEGIIPALESSHAVAYAMQLARTLRTDQSILVNLSGRGDKDIGTVAD 399

HSP 2 Score: 29.261 bits (64), Expect = 4.822e-41
Identity = 12/20 (60.00%), Postives = 15/20 (75.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH +I  E  RLIGV
Sbjct:  247 NAMGIFHPYIPHEKTRLIGV 266

HSP 3 Score: 24.2534 bits (51), Expect = 4.822e-41
Identity = 8/16 (50.00%), Postives = 11/16 (68.75%), Query Frame = -2 
Query:  699 PDVLLLCVGSGSKCVG 746
            PD ++ CVG GS  +G
Sbjct:  235 PDAVIACVGGGSNAMG 250
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_MYCLE (Tryptophan synthase beta chain OS=Mycobacterium leprae GN=trpB PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 6.271e-41
Identity = 72/134 (53.73%), Postives = 98/134 (73.13%), Query Frame = -1 
Query:  247 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 648
            EAAG G+++G+HAATL  G  G + G  SYLLQDE+GQ + +HS+  GL+YPGVGPE ++LR+TGRAE+   TD EA++A++ LCR EGI PALE++HA+A   KL   +  GA +VVN SG GDKD++    +
Sbjct:  277 EAAGDGVETGRHAATLTGGLPGAFQGTFSYLLQDEDGQTIESHSIAAGLDYPGVGPEHAWLRETGRAEYQPITDSEAIEAFRLLCRTEGILPALESAHAVAGALKLASEIGKGAVIVVNLSGRGDKDIEAAAKW 410

HSP 2 Score: 30.8018 bits (68), Expect = 6.271e-41
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+G+FH F++D  VRL+G
Sbjct:  257 NAIGIFHAFLDDPGVRLVG 275

HSP 3 Score: 24.2534 bits (51), Expect = 6.271e-41
Identity = 8/19 (42.11%), Postives = 11/19 (57.89%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD +  C+G GS  +G
Sbjct:  242 GRLPDAVTACIGGGSNAIG 260
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_EXIS2 (Tryptophan synthase beta chain OS=Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) GN=trpB PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 8.161e-41
Identity = 75/134 (55.97%), Postives = 97/134 (72.39%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG GL++ KHAAT++KGEVGV HG+M YLLQDE GQ+   HS+  GL+YPGVGPE S L+D GR ++   TDQ+A+ A Q LC+ EGI PALE++HA+A  ++L   +     VV+  SG GDKDV TV N
Sbjct:  260 IEAAGSGLETEKHAATMSKGEVGVLHGSMMYLLQDEHGQVTEAHSISAGLDYPGVGPEHSLLKDIGRVQYEAVTDQQALDALQLLCQKEGIIPALESAHAVAHAKELARGMQPEEVVVICLSGRGDKDVMTVRN 393

HSP 2 Score: 32.3426 bits (72), Expect = 8.161e-41
Identity = 12/20 (60.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH FI+DE+V L G+
Sbjct:  241 NAIGMFHPFIDDEEVALYGI 260

HSP 3 Score: 24.2534 bits (51), Expect = 8.161e-41
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  P+ ++ CVG GS  +G
Sbjct:  226 GKLPEAIVACVGGGSNAIG 244
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_POLNA5.899e-4254.48Tryptophan synthase beta chain OS=Polaromonas naph... [more]
TRPB_ACIF51.495e-4157.58Tryptophan synthase beta chain OS=Acidithiobacillu... [more]
TRPB_ACIF21.495e-4157.58Tryptophan synthase beta chain OS=Acidithiobacillu... [more]
TRPB_LEPCP2.193e-4156.72Tryptophan synthase beta chain OS=Leptothrix cholo... [more]
TRPB_ACICJ2.196e-4154.81Tryptophan synthase beta chain OS=Acidiphilium cry... [more]
TRPB_BORPD2.856e-4156.82Tryptophan synthase beta chain OS=Bordetella petri... [more]
TRPB_METCA3.712e-4153.79Tryptophan synthase beta chain OS=Methylococcus ca... [more]
TRPB_ACIAC4.822e-4155.22Tryptophan synthase beta chain OS=Acidovorax avena... [more]
TRPB_MYCLE6.271e-4153.73Tryptophan synthase beta chain OS=Mycobacterium le... [more]
TRPB_EXIS28.161e-4155.97Tryptophan synthase beta chain OS=Exiguobacterium ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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