CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_METFK (Tryptophan synthase beta chain OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=trpB PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.380e-40
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATL-AKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG G+D+G+HAA L A    GV HG  +YL+QDE GQI+ THS+  GL+YPGVGPE ++L+D  RAE+   TD EA++A+  LCR EGI PALE+SHALA+  KL  T+     ++VN SG GDKD++TV +
Sbjct:  260 VEAAGHGIDTGRHAAPLTANSPAGVLHGNRTYLMQDENGQIIETHSISAGLDYPGVGPEHAWLKDIKRAEYVAVTDDEAMEAFHTLCRTEGIIPALESSHALAYAMKLAETMSPDQALLVNLSGRGDKDINTVAS 394

HSP 2 Score: 28.8758 bits (63), Expect = 1.380e-40
Identity = 12/20 (60.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +I+ E VRLIGV
Sbjct:  241 NAMGIFYPYIDVEGVRLIGV 260

HSP 3 Score: 26.1794 bits (56), Expect = 1.380e-40
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  226 GRQPDAVIACVGGGSNAMG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NITMU (Tryptophan synthase beta chain OS=Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) GN=trpB PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.109e-40
Identity = 73/132 (55.30%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++ +HAATL KG  GV HG  +YL+QDE GQI+ THS+  GL+YPGVGPE ++L+D+GRAE++  TD++A++A+  LC  EGI PALE+SHALA+  +L P L +   ++VN SG GDKD+ TV
Sbjct:  259 VEAAGHGVETDQHAATLTKGRPGVLHGNRTYLIQDENGQIVETHSISAGLDYPGVGPEHAWLKDSGRAEYFGITDEQALEAFHALCHYEGIIPALESSHALAYAARLAPALTSDKLLLVNLSGRGDKDMPTV 390

HSP 2 Score: 28.1054 bits (61), Expect = 2.109e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD L+ CVG GS  +G
Sbjct:  226 GRQPDALIACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_MYCTU (Tryptophan synthase beta chain OS=Mycobacterium tuberculosis GN=trpB PE=1 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 3.028e-40
Identity = 70/134 (52.24%), Postives = 97/134 (72.39%), Query Frame = -1 
Query:  247 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 648
            EAAG G+++G+HAAT   G  G +HG+ SYLLQDE+GQ + +HS+  GL+YPGVGPE ++L++ GR ++   TD EA+ A+  LCR+EGI PA+E++HA+A   KL   L  GA +VVN SG GDKDV+T   +
Sbjct:  282 EAAGDGVETGRHAATFTAGSPGAFHGSFSYLLQDEDGQTIESHSISAGLDYPGVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGIIPAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETAAKW 415

HSP 2 Score: 30.8018 bits (68), Expect = 3.028e-40
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+G+FH F++D  VRL+G
Sbjct:  262 NAIGIFHAFLDDPGVRLVG 280

HSP 3 Score: 25.409 bits (54), Expect = 3.028e-40
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  247 GRLPDAVVACVGGGSNAIG 265
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_MYCBO (Tryptophan synthase beta chain OS=Mycobacterium bovis GN=trpB PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 3.028e-40
Identity = 70/134 (52.24%), Postives = 97/134 (72.39%), Query Frame = -1 
Query:  247 EAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 648
            EAAG G+++G+HAAT   G  G +HG+ SYLLQDE+GQ + +HS+  GL+YPGVGPE ++L++ GR ++   TD EA+ A+  LCR+EGI PA+E++HA+A   KL   L  GA +VVN SG GDKDV+T   +
Sbjct:  282 EAAGDGVETGRHAATFTAGSPGAFHGSFSYLLQDEDGQTIESHSISAGLDYPGVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGIIPAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETAAKW 415

HSP 2 Score: 30.8018 bits (68), Expect = 3.028e-40
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = -3 
Query:  653 NALGLFHEFINDEDVRLIG 709
            NA+G+FH F++D  VRL+G
Sbjct:  262 NAIGIFHAFLDDPGVRLVG 280

HSP 3 Score: 25.409 bits (54), Expect = 3.028e-40
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  247 GRLPDAVVACVGGGSNAIG 265
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_ALKMQ (Tryptophan synthase beta chain OS=Alkaliphilus metalliredigens (strain QYMF) GN=trpB PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 3.032e-40
Identity = 71/132 (53.79%), Postives = 98/132 (74.24%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++ +HAAT+ KG +GV HG M+YLLQDE+GQI   HS+  GL+YPG+GPE ++   TGRA +   TD+EA++A+Q L RLEGI PALE++HA+A+L KL P       +V+N SG GDKD+ T+
Sbjct:  258 VEAAGLGVETDQHAATITKGSMGVIHGMMTYLLQDEQGQITPVHSISAGLDYPGIGPEHAYYHHTGRANYVAITDEEALEAFQLLTRLEGIIPALESAHAIAYLMKLAPKTKGDDIIVLNLSGRGDKDIHTI 389

HSP 2 Score: 27.335 bits (59), Expect = 3.032e-40
Identity = 12/20 (60.00%), Postives = 14/20 (70.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F  DE V L GV
Sbjct:  239 NAMGLFYPFYEDEAVALYGV 258

HSP 3 Score: 23.483 bits (49), Expect = 3.032e-40
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  P+ L+ CVG GS  +G
Sbjct:  224 GRLPNYLVACVGGGSNAMG 242
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB1_PYRAB (Tryptophan synthase beta chain 1 OS=Pyrococcus abyssi GN=trpB1 PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 3.580e-40
Identity = 74/138 (53.62%), Postives = 103/138 (74.64%), Query Frame = -1 
Query:  238 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYRYN 651
            +EA G G++SGKH+A+L  GE+GV+HG +SY LQDEEGQI  THS+  GL+YPGVGPE ++L+++GRAE+ T TD+EA++A+  L R EGI PALE++HA+A+  KL   +     ++VN SG GDKD+D V+    N
Sbjct:  250 VEAGGKGIESGKHSASLNAGEIGVFHGMLSYFLQDEEGQIRTTHSIAPGLDYPGVGPEHAYLKESGRAEYVTVTDEEALRAFHELSRTEGIIPALESAHAVAYAIKLAREMSRDDVIIVNLSGRGDKDLDIVLKVSGN 387

HSP 2 Score: 26.9498 bits (58), Expect = 3.580e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PDV++ CVG GS  +G
Sbjct:  216 GDLPDVIVACVGGGSNAMG 234
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_POLSJ (Tryptophan synthase beta chain OS=Polaromonas sp. (strain JS666 / ATCC BAA-500) GN=trpB PE=3 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 5.110e-40
Identity = 72/134 (53.73%), Postives = 100/134 (74.63%), Query Frame = -1 
Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EAAG GL+SGKH+A+L +G  GV HG  +Y+LQ+++GQ+  THS+  GL+YPGVGPE +FL+D GRAE+   TD EA++A+  LCR EGI  ALE+SHA+A+  KL  T+ +   ++VN SG GDKD+ TV +
Sbjct:  261 VEAAGEGLESGKHSASLQRGLPGVLHGNRTYVLQNDDGQVTETHSISAGLDYPGVGPEHAFLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHAVAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVAD 394

HSP 2 Score: 28.1054 bits (61), Expect = 5.110e-40
Identity = 11/20 (55.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ +I  E+ RLIGV
Sbjct:  242 NAMGIFYPYIAHEETRLIGV 261

HSP 3 Score: 24.6386 bits (52), Expect = 5.110e-40
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = -2 
Query:  699 KPDVLLLCVGSGSKCVG 749
            +PD ++ CVG GS  +G
Sbjct:  229 QPDAVIACVGGGSNAMG 245
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NEIMF (Tryptophan synthase beta chain OS=Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) GN=trpB PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 5.124e-40
Identity = 70/137 (51.09%), Postives = 93/137 (67.88%), Query Frame = -1 
Query:  244 LEAAGFGLDSGKHAATLAKGE-VGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYR 651
            +EA G G+++  HAA +  G  +GV HG  SYL+QDE GQ+LGTHSV  GL+YPG+GPE S L D  R E+  A D EA++A+  LCR EGI PALE+SHA+A+  K  P +     ++VN SG GDKD++TV   +
Sbjct:  260 VEAGGLGVNTPDHAAPITSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAKDDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTVAKLK 396

HSP 2 Score: 33.8834 bits (76), Expect = 5.124e-40
Identity = 13/20 (65.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH +I +E+VRL+GV
Sbjct:  241 NAIGLFHPYIGEENVRLVGV 260

HSP 3 Score: 27.335 bits (59), Expect = 5.124e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PDV + CVG GS  +G
Sbjct:  226 GRQPDVAVACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NEIMB (Tryptophan synthase beta chain OS=Neisseria meningitidis serogroup B GN=trpB PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 5.124e-40
Identity = 70/137 (51.09%), Postives = 93/137 (67.88%), Query Frame = -1 
Query:  244 LEAAGFGLDSGKHAATLAKGE-VGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYR 651
            +EA G G+++  HAA +  G  +GV HG  SYL+QDE GQ+LGTHSV  GL+YPG+GPE S L D  R E+  A D EA++A+  LCR EGI PALE+SHA+A+  K  P +     ++VN SG GDKD++TV   +
Sbjct:  260 VEAGGLGVNTPDHAAPITSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAKDDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTVAKLK 396

HSP 2 Score: 33.8834 bits (76), Expect = 5.124e-40
Identity = 13/20 (65.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH +I +E+VRL+GV
Sbjct:  241 NAIGLFHPYIGEENVRLVGV 260

HSP 3 Score: 27.335 bits (59), Expect = 5.124e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PDV + CVG GS  +G
Sbjct:  226 GRQPDVAVACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NEIMA (Tryptophan synthase beta chain OS=Neisseria meningitidis serogroup A GN=trpB PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 5.124e-40
Identity = 70/137 (51.09%), Postives = 93/137 (67.88%), Query Frame = -1 
Query:  244 LEAAGFGLDSGKHAATLAKGE-VGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYR 651
            +EA G G+++  HAA +  G  +GV HG  SYL+QDE GQ+LGTHSV  GL+YPG+GPE S L D  R E+  A D EA++A+  LCR EGI PALE+SHA+A+  K  P +     ++VN SG GDKD++TV   +
Sbjct:  260 VEAGGLGVNTPDHAAPITSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAKDDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTVAKLK 396

HSP 2 Score: 33.8834 bits (76), Expect = 5.124e-40
Identity = 13/20 (65.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH +I +E+VRL+GV
Sbjct:  241 NAIGLFHPYIGEENVRLVGV 260

HSP 3 Score: 27.335 bits (59), Expect = 5.124e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PDV + CVG GS  +G
Sbjct:  226 GRQPDVAVACVGGGSNAIG 244
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_METFK1.380e-4054.81Tryptophan synthase beta chain OS=Methylobacillus ... [more]
TRPB_NITMU2.109e-4055.30Tryptophan synthase beta chain OS=Nitrosospira mul... [more]
TRPB_MYCTU3.028e-4052.24Tryptophan synthase beta chain OS=Mycobacterium tu... [more]
TRPB_MYCBO3.028e-4052.24Tryptophan synthase beta chain OS=Mycobacterium bo... [more]
TRPB_ALKMQ3.032e-4053.79Tryptophan synthase beta chain OS=Alkaliphilus met... [more]
TRPB1_PYRAB3.580e-4053.62Tryptophan synthase beta chain 1 OS=Pyrococcus aby... [more]
TRPB_POLSJ5.110e-4053.73Tryptophan synthase beta chain OS=Polaromonas sp. ... [more]
TRPB_NEIMF5.124e-4051.09Tryptophan synthase beta chain OS=Neisseria mening... [more]
TRPB_NEIMB5.124e-4051.09Tryptophan synthase beta chain OS=Neisseria mening... [more]
TRPB_NEIMA5.124e-4051.09Tryptophan synthase beta chain OS=Neisseria mening... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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