CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NEIM0 (Tryptophan synthase beta chain OS=Neisseria meningitidis serogroup C (strain 053442) GN=trpB PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 5.124e-40
Identity = 70/137 (51.09%), Postives = 93/137 (67.88%), Query Frame = -1 
Query:  244 LEAAGFGLDSGKHAATLAKGE-VGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYR 651
            +EA G G+++  HAA +  G  +GV HG  SYL+QDE GQ+LGTHSV  GL+YPG+GPE S L D  R E+  A D EA++A+  LCR EGI PALE+SHA+A+  K  P +     ++VN SG GDKD++TV   +
Sbjct:  260 VEAGGLGVNTPDHAAPITSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAKDDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTVAKLK 396

HSP 2 Score: 33.8834 bits (76), Expect = 5.124e-40
Identity = 13/20 (65.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH +I +E+VRL+GV
Sbjct:  241 NAIGLFHPYIGEENVRLVGV 260

HSP 3 Score: 27.335 bits (59), Expect = 5.124e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PDV + CVG GS  +G
Sbjct:  226 GRQPDVAVACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_THIDA (Tryptophan synthase beta chain OS=Thiobacillus denitrificans (strain ATCC 25259) GN=trpB PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 6.076e-40
Identity = 77/132 (58.33%), Postives = 95/132 (71.97%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G G+ SGKHAA L  G  GV HG  SYL+QDE GQI+ THSV  GL+YPGVGPE S+L+D GRAE+    D EA+ A+  LCR EGI PALE+SHA+A  +KL PT+     ++VN SG GDKD++TV
Sbjct:  260 VEAGGSGVASGKHAAPLTAGTPGVLHGFRSYLMQDENGQIIETHSVSAGLDYPGVGPEHSYLKDAGRAEYVPINDDEALAAFHDLCRFEGIIPALESSHAVAQAKKLAPTMKKDQVILVNLSGRGDKDINTV 391

HSP 2 Score: 26.5646 bits (57), Expect = 6.076e-40
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++ CVG GS  +G
Sbjct:  226 GRQPDAVVACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NEIG2 (Tryptophan synthase beta chain OS=Neisseria gonorrhoeae (strain NCCP11945) GN=trpB PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 6.661e-40
Identity = 71/137 (51.82%), Postives = 93/137 (67.88%), Query Frame = -1 
Query:  244 LEAAGFGLDSGKHAATLAKGE-VGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYR 651
            +EA G G+D+  HAA +  G  +GV HG  SYL+QDE GQ+LGTHSV  GL+YPG+GPE S L D  R E+  A D EA++A+  LCR EGI PALE+SHA+A+  K  P +     ++VN SG GDKD++TV   +
Sbjct:  260 VEAGGLGVDTPDHAAPITSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAKDDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTVAKLK 396

HSP 2 Score: 31.5722 bits (70), Expect = 6.661e-40
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ +I +E+VRL+GV
Sbjct:  241 NAIGLFYPYIEEENVRLVGV 260

HSP 3 Score: 27.335 bits (59), Expect = 6.661e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PDV + CVG GS  +G
Sbjct:  226 GRQPDVAVACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_NEIG1 (Tryptophan synthase beta chain OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=trpB PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 6.661e-40
Identity = 71/137 (51.82%), Postives = 93/137 (67.88%), Query Frame = -1 
Query:  244 LEAAGFGLDSGKHAATLAKGE-VGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNYR 651
            +EA G G+D+  HAA +  G  +GV HG  SYL+QDE GQ+LGTHSV  GL+YPG+GPE S L D  R E+  A D EA++A+  LCR EGI PALE+SHA+A+  K  P +     ++VN SG GDKD++TV   +
Sbjct:  260 VEAGGLGVDTPDHAAPITSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAKDDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTVAKLK 396

HSP 2 Score: 31.5722 bits (70), Expect = 6.661e-40
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ +I +E+VRL+GV
Sbjct:  241 NAIGLFYPYIEEENVRLVGV 260

HSP 3 Score: 27.335 bits (59), Expect = 6.661e-40
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PDV + CVG GS  +G
Sbjct:  226 GRQPDVAVACVGGGSNAIG 244
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_CLOAB (Tryptophan synthase beta chain OS=Clostridium acetobutylicum GN=trpB PE=3 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 6.664e-40
Identity = 72/133 (54.14%), Postives = 98/133 (73.68%), Query Frame = -1 
Query:  253 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVV 651
            +EAAG G+D+  HAATL KG VG+ HG M+Y+LQDE+GQI   +SV  GL+YPGVGP+ S+L++  RA +   TD+EA++A+  L   EGI PALE+SHA+A+  KL P+L     V++N SG GDKDV+TV+
Sbjct:  252 VEAAGLGIDTPMHAATLTKGSVGIIHGMMTYVLQDEDGQITPAYSVSAGLDYPGVGPQHSYLKEKERASYEAVTDKEALKAFLYLSEKEGIIPALESSHAVAYAMKLAPSLSKDEIVIINLSGRGDKDVNTVM 384

HSP 2 Score: 30.8018 bits (68), Expect = 6.664e-40
Identity = 12/20 (60.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            N++G+F+ FI DE V+LIGV
Sbjct:  233 NSMGIFYPFIKDEGVKLIGV 252

HSP 3 Score: 23.8682 bits (50), Expect = 6.664e-40
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  218 GRLPDYVVACVGGGSNSMG 236
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACWK (Tryptophan synthase beta chain OS=Bacillus weihenstephanensis (strain KBAB4) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.031e-39
Identity = 74/135 (54.81%), Postives = 99/135 (73.33%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD+EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDEEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.031e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACCR (Tryptophan synthase beta chain OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.031e-39
Identity = 74/135 (54.81%), Postives = 99/135 (73.33%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD+EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDEEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.031e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACC4 (Tryptophan synthase beta chain OS=Bacillus cereus (strain B4264) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.031e-39
Identity = 74/135 (54.81%), Postives = 99/135 (73.33%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD+EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDEEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.031e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB1_PYRKO (Tryptophan synthase beta chain 1 OS=Pyrococcus kodakaraensis GN=trpB1 PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.032e-39
Identity = 74/132 (56.06%), Postives = 101/132 (76.52%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EA G GL++G HAA+L  GE+GV+HG +SY LQ+EEGQI  THSV  GL+YPGVGPE ++L+D+GRAE+ T TD+EA++A+  L R EGI PALE++HA+A+  K+ P +     ++VN SG GDKD+D V
Sbjct:  252 VEAGGKGLETGLHAASLNAGELGVFHGMLSYFLQNEEGQITPTHSVSAGLDYPGVGPEHAYLKDSGRAEYVTVTDEEALRAFHELSRTEGILPALESAHAVAYAMKIAPEMDKDEIIIVNLSGRGDKDLDIV 383

HSP 2 Score: 24.6386 bits (52), Expect = 1.032e-39
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  219 GTLPDAVVACVGGGSNAMG 237
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LISIN (Tryptophan synthase beta chain OS=Listeria innocua GN=trpB PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 1.126e-39
Identity = 74/130 (56.92%), Postives = 97/130 (74.62%), Query Frame = -1 
Query:  262 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVD 651
            +EAAG GL++  HAAT++KGE+G+ HGAM  +LQDE GQIL   S+  GL+YPG+GPE SF RD GRAE+++ TD EAV+A+Q LCR EGI PALE+SHA+++  KL   +     +VV  SG GDKDV+
Sbjct:  259 VEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFRDLGRAEYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPDESMVVCLSGRGDKDVN 388

HSP 2 Score: 27.335 bits (59), Expect = 1.126e-39
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F++D  V++ GV
Sbjct:  240 NAMGLFYPFVDDSSVQMHGV 259

HSP 3 Score: 23.483 bits (49), Expect = 1.126e-39
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  P+ ++ CVG GS  +G
Sbjct:  225 GKLPEAIVACVGGGSNAMG 243
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_NEIM05.124e-4051.09Tryptophan synthase beta chain OS=Neisseria mening... [more]
TRPB_THIDA6.076e-4058.33Tryptophan synthase beta chain OS=Thiobacillus den... [more]
TRPB_NEIG26.661e-4051.82Tryptophan synthase beta chain OS=Neisseria gonorr... [more]
TRPB_NEIG16.661e-4051.82Tryptophan synthase beta chain OS=Neisseria gonorr... [more]
TRPB_CLOAB6.664e-4054.14Tryptophan synthase beta chain OS=Clostridium acet... [more]
TRPB_BACWK1.031e-3954.81Tryptophan synthase beta chain OS=Bacillus weihens... [more]
TRPB_BACCR1.031e-3954.81Tryptophan synthase beta chain OS=Bacillus cereus ... [more]
TRPB_BACC41.031e-3954.81Tryptophan synthase beta chain OS=Bacillus cereus ... [more]
TRPB1_PYRKO1.032e-3956.06Tryptophan synthase beta chain 1 OS=Pyrococcus kod... [more]
TRPB_LISIN1.126e-3956.92Tryptophan synthase beta chain OS=Listeria innocua... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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