CN185134 | Citrus Genome Database

Overview


Name	CN185134
Unique Name	CN185134
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	756

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LISW6 (Tryptophan synthase beta chain OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.464e-39
Identity = 74/130 (56.92%), Postives = 97/130 (74.62%), Query Frame = -1

Query:  262 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVD 651
            +EAAG GL++  HAAT++KGE+G+ HGAM  +LQDE GQIL   S+  GL+YPG+GPE SF RD GRAE+++ TD EAV+A+Q LCR EGI PALE+SHA+++  KL   +     +VV  SG GDKDV+
Sbjct:  259 VEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFRDLGRAEYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASKMRPEESMVVCLSGRGDKDVN 388

HSP 2 Score: 27.335 bits (59), Expect = 1.464e-39
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F++D  V++ GV
Sbjct:  240 NAMGLFYPFVDDSSVQMHGV 259

HSP 3 Score: 23.483 bits (49), Expect = 1.464e-39
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  P+ ++ CVG GS  +G
Sbjct:  225 GKLPEAIVACVGGGSNAMG 243

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACHK (Tryptophan synthase beta chain OS=Bacillus thuringiensis subsp. konkukian GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACCZ (Tryptophan synthase beta chain OS=Bacillus cereus (strain ZK / E33L) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPKMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACCQ (Tryptophan synthase beta chain OS=Bacillus cereus (strain Q1) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACC7 (Tryptophan synthase beta chain OS=Bacillus cereus (strain AH187) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKKDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACC3 (Tryptophan synthase beta chain OS=Bacillus cereus (strain 03BB102) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACC2 (Tryptophan synthase beta chain OS=Bacillus cereus (strain G9842) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPKMKKDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACAN (Tryptophan synthase beta chain OS=Bacillus anthracis GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACAH (Tryptophan synthase beta chain OS=Bacillus thuringiensis (strain Al Hakam) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACAC (Tryptophan synthase beta chain OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

The following BLAST results are available for this feature:

BLAST of CN185134 vs. ExPASy Swiss-Prot

BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427


Match Name	E-value	Identity	Description
TRPB_LISW6	1.464e-39	56.92	Tryptophan synthase beta chain OS=Listeria welshim...	[more]
TRPB_BACHK	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus thuring...	[more]
TRPB_BACCZ	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus cereus ...	[more]
TRPB_BACCQ	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus cereus ...	[more]
TRPB_BACC7	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus cereus ...	[more]
TRPB_BACC3	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus cereus ...	[more]
TRPB_BACC2	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus cereus ...	[more]
TRPB_BACAN	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus anthrac...	[more]
TRPB_BACAH	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus thuring...	[more]
TRPB_BACAC	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus anthrac...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC