CN185134 | Citrus Genome Database

Overview


Name	CN185134
Unique Name	CN185134
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	756

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BACAA (Tryptophan synthase beta chain OS=Bacillus anthracis (strain A0248) GN=trpB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.751e-39
Identity = 74/135 (54.81%), Postives = 98/135 (72.59%), Query Frame = -1

Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+ + KHAATL KG VGV HG+M YLLQ+EEGQI   HS+  GL+YPGVGPE S L+D GR  +++ TD EA++A+Q L + EGI PALE+SHA+A+  KL P +     +V+  SG GDKDV+++  Y
Sbjct:  259 VEAAGKGVHTEKHAATLTKGSVGVLHGSMMYLLQNEEGQIQEAHSISAGLDYPGVGPEHSLLKDIGRVSYHSITDDEALEAFQLLTKKEGIIPALESSHAVAYALKLAPQMKEDEGLVICLSGRGDKDVESIKRY 393

HSP 2 Score: 30.0314 bits (66), Expect = 1.751e-39
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+F+ F++DE+V L GV
Sbjct:  240 NAMGMFYPFVHDEEVALYGV 259

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_CAUCR (Tryptophan synthase beta chain OS=Caulobacter crescentus GN=trpB PE=3 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 1.902e-39
Identity = 70/132 (53.03%), Postives = 93/132 (70.45%), Query Frame = -1

Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+ + KHAA+L  G  GV HG  +YLLQD++GQI+  HS+  GL+YPG+GPE SFL D GRAE+ + TD EA++A++    LEGI PALE +HALA + ++   L  G  VV+N  G GDKD+ TV
Sbjct:  267 VEAAGHGVSTDKHAASLTGGRPGVLHGNRTYLLQDDDGQIIDAHSISAGLDYPGIGPEHSFLHDIGRAEYVSTTDTEALEAFKLCSTLEGIIPALEPAHALARVGEIAQELGKGKIVVMNLCGRGDKDIFTV 398

HSP 2 Score: 35.4242 bits (80), Expect = 1.902e-39
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH F+ DE VRLIGV
Sbjct:  248 NAIGLFHPFLGDEGVRLIGV 267

HSP 3 Score: 25.0238 bits (53), Expect = 1.902e-39
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ C+G GS  +G
Sbjct:  233 GRLPDAVVACIGGGSNAIG 251

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_RUTMC (Tryptophan synthase beta chain OS=Ruthia magnifica subsp. Calyptogena magnifica GN=trpB PE=3 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 2.473e-39
Identity = 67/134 (50.00%), Postives = 96/134 (71.64%), Query Frame = -1

Query:  256 LEAAGFGLDSGK--HAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG+GL+ G   H+A L  G VGV HG  +YL++DE GQI+  HS+  GL+YPGVGPE ++L+D+GRA +   TD+EA++A+  L ++EGI PALE+SHA+A+  KL   L     +++N SG GDKD+ T+
Sbjct:  260 VEAAGYGLEKGPTAHSAPLCAGSVGVLHGNRTYLMEDENGQIIEGHSISAGLDYPGVGPEHAYLKDSGRARYVAITDKEALEAFHVLTKVEGILPALESSHAVAYGIKLAKELGKDKHIIINLSGRGDKDIHTI 393

HSP 2 Score: 31.187 bits (69), Expect = 2.473e-39
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PDVL+ CVG GS  +G
Sbjct:  226 GGLPDVLVACVGGGSNAIG 244

HSP 3 Score: 30.0314 bits (66), Expect = 2.473e-39
Identity = 12/20 (60.00%), Postives = 15/20 (75.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFH FI+D  V + GV
Sbjct:  241 NAIGLFHAFIDDASVEIYGV 260

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB2_CHLCV (Tryptophan synthase beta chain 2 OS=Chlamydophila caviae GN=trpB2 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 2.973e-39
Identity = 75/134 (55.97%), Postives = 96/134 (71.64%), Query Frame = -1

Query:  250 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVN 651
            +EA G G++SG HAA  A G  GV HG  SYLLQD EGQ+L THS+  GL+YP VGP+ + L ++GRA +  ATD+ A++A+  L RLEGI PALE+SHALA L  + PTLP  + VVVN SG GDKD++ + N
Sbjct:  250 VEAGGLGIESGHHAARFATGRPGVLHGFYSYLLQDNEGQVLPTHSISAGLDYPAVGPDHAVLYESGRASYTVATDKAALEAFFLLSRLEGIIPALESSHALAELINIAPTLPKESVVVVNLSGRGDKDLEQITN 383

HSP 2 Score: 30.0314 bits (66), Expect = 2.973e-39
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = -2

Query:  696 GGKPDVLLLCVGSGSKCVGF 755
            G  PD+L+ CVG GS  +GF
Sbjct:  216 GFSPDLLIACVGGGSNAIGF 235

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LISMO (Tryptophan synthase beta chain OS=Listeria monocytogenes GN=trpB PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 3.215e-39
Identity = 73/130 (56.15%), Postives = 96/130 (73.85%), Query Frame = -1

Query:  262 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVD 651
            +EAAG GL++  HAAT++KGE+G+ HGAM  +LQDE GQIL   S+  GL+YPG+GPE SF RD GRA +++ TD EAV+A+Q LCR EGI PALE+SHA+++  KL   +     +VV  SG GDKDV+
Sbjct:  259 VEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFRDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASKMRPEESMVVCLSGRGDKDVN 388

HSP 2 Score: 26.9498 bits (58), Expect = 3.215e-39
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F++D  V++ GV
Sbjct:  240 NAMGLFYPFVDDASVQMHGV 259

HSP 3 Score: 25.0238 bits (53), Expect = 3.215e-39
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  225 GKLPDAIVACVGGGSNAMG 243

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LISMH (Tryptophan synthase beta chain OS=Listeria monocytogenes serotype 4a (strain HCC23) GN=trpB PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 4.180e-39
Identity = 73/130 (56.15%), Postives = 96/130 (73.85%), Query Frame = -1

Query:  262 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVD 651
            +EAAG GL++  HAAT++KGE+G+ HGAM  +LQDE GQIL   S+  GL+YPG+GPE SF RD GRA +++ TD EAV+A+Q LCR EGI PALE+SHA+++  KL   +     +VV  SG GDKDV+
Sbjct:  259 VEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFRDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDVN 388

HSP 2 Score: 26.5646 bits (57), Expect = 4.180e-39
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F++D  V++ GV
Sbjct:  240 NAMGLFYPFVDDVSVQMHGV 259

HSP 3 Score: 25.0238 bits (53), Expect = 4.180e-39
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  225 GKLPDAIVACVGGGSNAMG 243

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LISMF (Tryptophan synthase beta chain OS=Listeria monocytogenes serotype 4b (strain F2365) GN=trpB PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 4.180e-39
Identity = 73/130 (56.15%), Postives = 96/130 (73.85%), Query Frame = -1

Query:  262 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVD 651
            +EAAG GL++  HAAT++KGE+G+ HGAM  +LQDE GQIL   S+  GL+YPG+GPE SF RD GRA +++ TD EAV+A+Q LCR EGI PALE+SHA+++  KL   +     +VV  SG GDKDV+
Sbjct:  259 VEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFRDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDVN 388

HSP 2 Score: 26.5646 bits (57), Expect = 4.180e-39
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F++D  V++ GV
Sbjct:  240 NAMGLFYPFVDDVSVQMHGV 259

HSP 3 Score: 25.0238 bits (53), Expect = 4.180e-39
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  225 GKLPDAIVACVGGGSNAMG 243

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_LISMC (Tryptophan synthase beta chain OS=Listeria monocytogenes serotype 4b (strain Clip81459) GN=trpB PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 4.180e-39
Identity = 73/130 (56.15%), Postives = 96/130 (73.85%), Query Frame = -1

Query:  262 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVD 651
            +EAAG GL++  HAAT++KGE+G+ HGAM  +LQDE GQIL   S+  GL+YPG+GPE SF RD GRA +++ TD EAV+A+Q LCR EGI PALE+SHA+++  KL   +     +VV  SG GDKDV+
Sbjct:  259 VEAAGHGLETEFHAATISKGEIGILHGAMMDVLQDENGQILEAFSISAGLDYPGIGPEHSFFRDLGRAAYHSVTDDEAVEAFQLLCRTEGIIPALESSHAISYAVKLASQMRPEESMVVCLSGRGDKDVN 388

HSP 2 Score: 26.5646 bits (57), Expect = 4.180e-39
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+ F++D  V++ GV
Sbjct:  240 NAMGLFYPFVDDVSVQMHGV 259

HSP 3 Score: 25.0238 bits (53), Expect = 4.180e-39
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G  PD ++ CVG GS  +G
Sbjct:  225 GKLPDAIVACVGGGSNAMG 243

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BORPA (Tryptophan synthase beta chain OS=Bordetella parapertussis GN=trpB PE=3 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.180e-39
Identity = 69/132 (52.27%), Postives = 99/132 (75.00%), Query Frame = -1

Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++G+HAA+LA G++GV HG  +Y++Q+ +GQ+  THSV  GL+YPGVGPE ++L+D+GRAE+   TD EA+ A+   CR+EGI PALE++HA+A   K+ P L     ++VN SG GDKD+ TV
Sbjct:  260 VEAAGEGMETGRHAASLAAGQIGVLHGNRTYVMQNADGQVQETHSVSAGLDYPGVGPEHAWLKDSGRAEYAGITDDEALAAFHDCCRIEGIMPALESAHAIAQAVKMAPALSKDKVILVNLSGRGDKDMHTV 391

HSP 2 Score: 30.8018 bits (68), Expect = 4.180e-39
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH +I  E+VRLIGV
Sbjct:  241 NAMGIFHPYIPYENVRLIGV 260

HSP 3 Score: 21.9422 bits (45), Expect = 4.180e-39
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++  VG GS  +G
Sbjct:  226 GRQPDYVVAAVGGGSNAMG 244

[Close]

BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_BORBR (Tryptophan synthase beta chain OS=Bordetella bronchiseptica GN=trpB PE=3 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.180e-39
Identity = 69/132 (52.27%), Postives = 99/132 (75.00%), Query Frame = -1

Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+++G+HAA+LA G++GV HG  +Y++Q+ +GQ+  THSV  GL+YPGVGPE ++L+D+GRAE+   TD EA+ A+   CR+EGI PALE++HA+A   K+ P L     ++VN SG GDKD+ TV
Sbjct:  260 VEAAGEGMETGRHAASLAAGQIGVLHGNRTYVMQNADGQVQETHSVSAGLDYPGVGPEHAWLKDSGRAEYAGITDDEALAAFHDCCRIEGIMPALESAHAIAQAVKMAPALSKDKVILVNLSGRGDKDMHTV 391

HSP 2 Score: 30.8018 bits (68), Expect = 4.180e-39
Identity = 13/20 (65.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+G+FH +I  E+VRLIGV
Sbjct:  241 NAMGIFHPYIPYENVRLIGV 260

HSP 3 Score: 21.9422 bits (45), Expect = 4.180e-39
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = -2

Query:  699 GGKPDVLLLCVGSGSKCVG 755
            G +PD ++  VG GS  +G
Sbjct:  226 GRQPDYVVAAVGGGSNAMG 244

The following BLAST results are available for this feature:

BLAST of CN185134 vs. ExPASy Swiss-Prot

BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427


Match Name	E-value	Identity	Description
TRPB_BACAA	1.751e-39	54.81	Tryptophan synthase beta chain OS=Bacillus anthrac...	[more]
TRPB_CAUCR	1.902e-39	53.03	Tryptophan synthase beta chain OS=Caulobacter cres...	[more]
TRPB_RUTMC	2.473e-39	50.00	Tryptophan synthase beta chain OS=Ruthia magnifica...	[more]
TRPB2_CHLCV	2.973e-39	55.97	Tryptophan synthase beta chain 2 OS=Chlamydophila ...	[more]
TRPB_LISMO	3.215e-39	56.15	Tryptophan synthase beta chain OS=Listeria monocyt...	[more]
TRPB_LISMH	4.180e-39	56.15	Tryptophan synthase beta chain OS=Listeria monocyt...	[more]
TRPB_LISMF	4.180e-39	56.15	Tryptophan synthase beta chain OS=Listeria monocyt...	[more]
TRPB_LISMC	4.180e-39	56.15	Tryptophan synthase beta chain OS=Listeria monocyt...	[more]
TRPB_BORPA	4.180e-39	52.27	Tryptophan synthase beta chain OS=Bordetella parap...	[more]
TRPB_BORBR	4.180e-39	52.27	Tryptophan synthase beta chain OS=Bordetella bronc...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC