EY666500

Overview
NameEY666500
Unique NameEY666500
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length851
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATM_PIG (Aspartate aminotransferase, mitochondrial OS=Sus scrofa GN=GOT2 PE=1 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 3.348e-22
Identity = 51/96 (53.12%), Postives = 64/96 (66.67%), Query Frame = 3 
Query:  294 GRVSMVAAVNVSRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLMLERGENKEYLPIESLAAFNKVTAELLFG 581
            G  +  +A   S +  V M PPDPILGV+EAFK DT+ KK+NLGVGAYR +  +PYVL  V+KAE  +  +  +KEYLPI  LA F K +AEL  G
Sbjct:   20 GLAAAASARASSWWAHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALG 115

HSP 2 Score: 30.8018 bits (68), Expect = 3.348e-22
Identity = 16/42 (38.10%), Postives = 25/42 (59.52%), Query Frame = 2 
Query:  623 LQGLSRTGSLRVAAALIQRYFPGEKD*Y-HLPHGGNHKKHFQ 745
            +Q +S TG+LR+ A  +QR+F   +D +   P  GNH   F+
Sbjct:  129 VQTISGTGALRIGANFLQRFFKFSRDVFLPKPSWGNHTPIFR 170
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATM_HORSE (Aspartate aminotransferase, mitochondrial OS=Equus caballus GN=GOT2 PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.675e-22
Identity = 47/80 (58.75%), Postives = 58/80 (72.50%), Query Frame = 3 
Query:  342 VTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLMLERGENKEYLPIESLAAFNKVTAELLFG 581
            V M PPDPILGV+EA+K DT+ KK+NLGVGAYR +  +PYVL  V+KAE  +  +  +KEYLPI  LA F K +AEL  G
Sbjct:    7 VEMGPPDPILGVTEAYKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALG 86

HSP 2 Score: 31.5722 bits (70), Expect = 5.675e-22
Identity = 16/42 (38.10%), Postives = 25/42 (59.52%), Query Frame = 2 
Query:  623 LQGLSRTGSLRVAAALIQRYFPGEKD*Y-HLPHGGNHKKHFQ 745
            +Q +S TG+LR+ A  +QR+F   +D +   P  GNH   F+
Sbjct:  100 VQSISGTGALRIGANFLQRFFKFSRDVFLPKPSWGNHTPIFR 141
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATC_DAUCA (Aspartate aminotransferase, cytoplasmic OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 8.904e-22
Identity = 50/87 (57.47%), Postives = 64/87 (73.56%), Query Frame = 3 
Query:  327 SRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLML-ERGENKEYLPIESLAAFNKVTAELLFGS 584
            S F  V  AP DPILGV+ A+  D    KLNLGVGAYRTEE +P VL+VVKKAE +++ ++   KEYLPI  LA FNK++A+L+FG+
Sbjct:    3 SVFANVVRAPEDPILGVTVAYHKDQSPNKLNLGVGAYRTEEGKPLVLNVVKKAEQMLVNDQSRVKEYLPIVGLADFNKLSAKLIFGA 89

HSP 2 Score: 28.1054 bits (61), Expect = 8.904e-22
Identity = 16/47 (34.04%), Postives = 23/47 (48.94%), Query Frame = 2 
Query:  602 RKQELQQLQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            ++  +  +Q LS TGSLRV    + R++         P  GNH K F
Sbjct:   95 QENRVATVQCLSGTGSLRVGGEFLARHYHEHTVYIPQPTWGNHPKIF 141

HSP 3 Score: 21.557 bits (44), Expect = 8.904e-22
Identity = 9/15 (60.00%), Postives = 12/15 (80.00%), Query Frame = 1 
Query:  580 GADNSVLKEARVATV 624
            GAD+  ++E RVATV
Sbjct:   88 GADSPAIQENRVATV 102
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATC_ORYSJ (Aspartate aminotransferase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os01g0760600 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 4.227e-21
Identity = 47/87 (54.02%), Postives = 63/87 (72.41%), Query Frame = 3 
Query:  327 SRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLMLERGEN-KEYLPIESLAAFNKVTAELLFGS 584
            S F G+  AP DPILGV+ A+  D    K+NLGVGAYRTEE +P VL+VV++AE +++      KEYLPI  LA FNK++A+L+FG+
Sbjct:    5 SVFAGLAQAPEDPILGVTVAYNKDPSPVKVNLGVGAYRTEEGKPLVLNVVRRAEQMLINNPSRVKEYLPITGLADFNKLSAKLIFGA 91

HSP 2 Score: 28.1054 bits (61), Expect = 4.227e-21
Identity = 16/47 (34.04%), Postives = 23/47 (48.94%), Query Frame = 2 
Query:  602 RKQELQQLQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            ++  +  +Q LS TGSLRV    + R++         P  GNH K F
Sbjct:   97 QENRVATVQCLSGTGSLRVGGEFLARHYHERTIYIPQPTWGNHPKVF 143

HSP 3 Score: 21.557 bits (44), Expect = 4.227e-21
Identity = 9/15 (60.00%), Postives = 12/15 (80.00%), Query Frame = 1 
Query:  580 GADNSVLKEARVATV 624
            GAD+  ++E RVATV
Sbjct:   90 GADSPAIQENRVATV 104
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AAT1_MEDSA (Aspartate aminotransferase 1 OS=Medicago sativa GN=AAT-1 PE=2 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 6.051e-21
Identity = 48/87 (55.17%), Postives = 62/87 (71.26%), Query Frame = 3 
Query:  327 SRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLML-ERGENKEYLPIESLAAFNKVTAELLFGS 584
            S F  +  AP DPILGV+ A+  D    KLNLGVGAYRTEE +P VLDVV++ E  +L +   NKEY+PI  LA FNK++A+L+FG+
Sbjct:   16 SVFAHLVRAPEDPILGVTVAYNKDPSPIKLNLGVGAYRTEEGKPLVLDVVRRVERQLLNDMSRNKEYIPIVGLADFNKLSAKLIFGA 102

HSP 2 Score: 30.0314 bits (66), Expect = 6.051e-21
Identity = 16/47 (34.04%), Postives = 24/47 (51.06%), Query Frame = 2 
Query:  602 RKQELQQLQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            ++  +  +QGLS TGSLRV    + +++         P  GNH K F
Sbjct:  108 QENRVTTVQGLSGTGSLRVGGEFLAKHYHQRIIYLPTPTWGNHTKVF 154
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATM_DICDI (Aspartate aminotransferase, mitochondrial OS=Dictyostelium discoideum GN=aatA PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.956e-20
Identity = 48/107 (44.86%), Postives = 65/107 (60.75%), Query Frame = 3 
Query:  264 TAGFIKTKSFGRVSMVA-AVNVSRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLMLERGENKEYLPIESLAAFNKVTAELLFG 581
            +A  I    FG+ ++   + N + +  V   P DPILGVS A+  DT   K+NLGVGAYR E  +PYVLD VKKA+  + E   + EY PI  +AAFN++ A+L  G
Sbjct:    4 SARLISNIKFGQKNIRQFSTNTNWWANVQKGPEDPILGVSIAYNKDTSPSKINLGVGAYRDENGKPYVLDCVKKADKKIYEANVDHEYAPIVGVAAFNQLAAQLALG 110

HSP 2 Score: 30.8018 bits (68), Expect = 4.956e-20
Identity = 15/47 (31.91%), Postives = 25/47 (53.19%), Query Frame = 2 
Query:  602 RKQELQQLQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            +++ +  +Q +S TG+LR+AA    R+  G+      P  GNH   F
Sbjct:  117 KEKRIATVQSISGTGALRIAADFFARFLKGKTAYVPNPTWGNHNVIF 163
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AAT3_ARATH (Aspartate aminotransferase, chloroplastic OS=Arabidopsis thaliana GN=ASP3 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.396e-19
Identity = 48/87 (55.17%), Postives = 61/87 (70.11%), Query Frame = 3 
Query:  327 SRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAE-NLMLERGENKEYLPIESLAAFNKVTAELLFGS 584
            S F  +  AP DPILGV+ A+  D    KLNLGVGAYRTEE +P VL+VV+KAE  L+ +R   KEYLPI  L  FNK++A+L+ G+
Sbjct:   47 SVFSHLVQAPEDPILGVTVAYNKDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAKLILGA 133

HSP 2 Score: 28.1054 bits (61), Expect = 2.396e-19
Identity = 15/47 (31.91%), Postives = 24/47 (51.06%), Query Frame = 2 
Query:  602 RKQELQQLQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            R+  +  ++ LS TGSLRV    + +++  +      P  GNH K F
Sbjct:  139 RENRITTVECLSGTGSLRVGGEFLAKHYHQKTIYITQPTWGNHPKIF 185
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATC_DICDI (Aspartate aminotransferase, cytoplasmic OS=Dictyostelium discoideum GN=aatB PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 4.054e-19
Identity = 46/83 (55.42%), Postives = 59/83 (71.08%), Query Frame = 3 
Query:  333 FEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLMLERGENKEYLPIESLAAFNKVTAELLFG 581
            F  V + P DPILGVS AFKADTD +K++  VGAYR E  +PYVL  V +AE  +L  G  KEYLPI+ +  FNK++A+LL+G
Sbjct:   41 FANVPLGPVDPILGVSTAFKADTDPRKVDTSVGAYRDENGKPYVLKCVFEAEKRLL--GAPKEYLPIDGIPEFNKLSAKLLYG 121

HSP 2 Score: 26.9498 bits (58), Expect = 4.054e-19
Identity = 10/29 (34.48%), Postives = 19/29 (65.52%), Query Frame = 2 
Query:  602 RKQELQQLQGLSRTGSLRVAAALIQRYFP 688
            +++ +  +Q LS TG+LR+    I++Y P
Sbjct:  127 KEKRMVTVQALSGTGALRIGIIFIRKYLP 155
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AAT2_ARATH (Aspartate aminotransferase, cytoplasmic isozyme 1 OS=Arabidopsis thaliana GN=ASP2 PE=1 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 8.930e-19
Identity = 46/87 (52.87%), Postives = 61/87 (70.11%), Query Frame = 3 
Query:  327 SRFEGVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAE-NLMLERGENKEYLPIESLAAFNKVTAELLFGS 584
            S F  V  AP DPILGV+ A+  D    K+NLGVGAYRTEE +P VLDVV+KAE  L+ +    KEY+PI  ++ FNK++A+L+ G+
Sbjct:    3 SVFSNVARAPEDPILGVTVAYNNDPSPVKINLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPSRVKEYIPIVGISDFNKLSAKLILGA 89

HSP 2 Score: 27.335 bits (59), Expect = 8.930e-19
Identity = 16/40 (40.00%), Postives = 21/40 (52.50%), Query Frame = 2 
Query:  623 LQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            +Q LS TGSLRV A  ++ ++         P  GNH K F
Sbjct:  102 VQCLSGTGSLRVGAEFLKTHYHQSVIYIPKPTWGNHPKVF 141
[Close]
BLAST of EY666500 vs. ExPASy Swiss-Prot
Match: AATM_SCHPO (Aspartate aminotransferase, mitochondrial OS=Schizosaccharomyces pombe GN=aat1 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.601e-17
Identity = 43/111 (38.74%), Postives = 64/111 (57.66%), Query Frame = 3 
Query:  258 NSTAGFIKTKSFGRVSMVAAVNVSRFE---GVTMAPPDPILGVSEAFKADTDEKKLNLGVGAYRTEELQPYVLDVVKKAENLMLERGENKEYLPIESLAAFNKVTAELLFG 581
            N+ A      S+  V  +A+ +   F+    V M PPDPI G++EA+K D D KK+NLG G YR +  +PYVL  V++AE  +L +  +KEY PI  + +F     +L +G
Sbjct:   12 NTFASLKTNVSYHGVKCLASQSKRGFKVWADVPMGPPDPIFGITEAYKKDGDVKKMNLGAGTYRDDAGKPYVLPSVRQAETELLSQKLDKEYAPITGIPSFRVQATKLAYG 122

HSP 2 Score: 30.8018 bits (68), Expect = 1.601e-17
Identity = 16/46 (34.78%), Postives = 23/46 (50.00%), Query Frame = 2 
Query:  605 KQELQQLQGLSRTGSLRVAAALIQRYFPGEKD*YHLPHGGNHKKHF 742
            K  L   Q +S TG+L +AA  +  ++P +      P  GNHK  F
Sbjct:  129 KDRLVSAQSISGTGALCIAANFLASFYPSKTIYVSDPTWGNHKNVF 174
The following BLAST results are available for this feature:
BLAST of EY666500 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 38
Match NameE-valueIdentityDescription
AATM_PIG3.348e-2253.13Aspartate aminotransferase, mitochondrial OS=Sus s... [more]
AATM_HORSE5.675e-2258.75Aspartate aminotransferase, mitochondrial OS=Equus... [more]
AATC_DAUCA8.904e-2257.47Aspartate aminotransferase, cytoplasmic OS=Daucus ... [more]
AATC_ORYSJ4.227e-2154.02Aspartate aminotransferase, cytoplasmic OS=Oryza s... [more]
AAT1_MEDSA6.051e-2155.17Aspartate aminotransferase 1 OS=Medicago sativa GN... [more]
AATM_DICDI4.956e-2044.86Aspartate aminotransferase, mitochondrial OS=Dicty... [more]
AAT3_ARATH2.396e-1955.17Aspartate aminotransferase, chloroplastic OS=Arabi... [more]
AATC_DICDI4.054e-1955.42Aspartate aminotransferase, cytoplasmic OS=Dictyos... [more]
AAT2_ARATH8.930e-1952.87Aspartate aminotransferase, cytoplasmic isozyme 1 ... [more]
AATM_SCHPO1.601e-1738.74Aspartate aminotransferase, mitochondrial OS=Schiz... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-018-E09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY666500 ID=EY666500; Name=EY666500; organism=Citrus sinensis; type=EST; length=851bp
TTCAACGTACTTGTTGCTCCATAGCAAGGAGATCGCACACGCACAGCTTC
GTAATAGTCGCCATTTTCTCGTGAAATCTCTTTACCATCAGTGAATAAGC
TACTTCGATTAAGTCAAGGAGAGCTTAAGCGTTCAATTTTTATCGTAAAA
TCACATAGATGGCCTCAACAATGCTTTCAATAGCTTCTGCAACTCCTTCA
GCTTCGCTATCGATGCACGAGAAGCTCAAGGGCAAGGTTAAGCTTGGAAG
TACCACTAATTCGACAGCAGGATTCATTAAGACTAAATCCTTTGGACGAG
TATCTATGGTTGCTGCTGTCAATGTTTCTCGTTTTGAGGGTGTAACGATG
GCTCCCCCCGATCCAATTCTTGGAGTTTCTGAAGCTTTTAAAGCTGACAC
AGATGAAAAGAAACTCAACCTTGGAGTCGGTGCTTATCGAACCGAAGAGC
TACAGCCTTATGTGCTTGATGTTGTTAAGAAGGCAGAGAATCTTATGCTG
GAAAGGGGGGAAAACAAGGAGTATCTTCCAATTGAAAGTTTGGCTGCATT
CAATAAGGTGACAGCAGAGTTATTGTTTGGGAGCTGACAACTCAGTGCTA
AAGGAAGCAAGAGTTGCAACAGTTGCAAGGTCTTTCAAGAACCGGATCTC
TTCGGGTGGCTGCAGCTCTTATACAACGATATTTTCCTGGGGAAAAGGAC
TAATATCATCTTCCACATGGGGGGAACCACAAGAAACATTTTCAATGATG
CTAAAATTTCATGGGCTGGATAACCGCTACTTTTAATCCAAAAAAGGGGG
GGTTGGGATTTTGAGGGAAAGGAAACCCAAAATAAAAGGGCGCCCCCCGG
G
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