CN184837

Overview
NameCN184837
Unique NameCN184837
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length773
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.168e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 95.1301 bits (235), Expect = 4.439e-19
Identity = 46/76 (60.53%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I L++E  D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.526e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = -2 
Query:  407 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 631
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 93.9745 bits (232), Expect = 9.888e-19
Identity = 46/74 (62.16%), Postives = 57/74 (77.03%), Query Frame = -2 
Query:  182 IFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 403
            I VKTLTGK I +++E  DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.291e-18
Identity = 42/78 (53.85%), Postives = 59/78 (75.64%), Query Frame = -2 
Query:  176 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGDF 409
            + + V+TLTGK I +++E  DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG+F
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGGNF 78

HSP 2 Score: 92.8189 bits (229), Expect = 2.203e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.687e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.2781 bits (225), Expect = 6.409e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I +++E  D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.687e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.2781 bits (225), Expect = 6.409e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I +++E  D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.687e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.2781 bits (225), Expect = 6.409e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I +++E  D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.687e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.2781 bits (225), Expect = 6.409e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I +++E  D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.687e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.2781 bits (225), Expect = 6.409e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I +++E  D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.428e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  410 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 637
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 88.1965 bits (217), Expect = 5.426e-17
Identity = 42/76 (55.26%), Postives = 56/76 (73.68%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M+I VKTLT K I +E+E  DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of CN184837 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.865e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = -2 
Query:  407 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 637
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 87.0409 bits (214), Expect = 1.209e-16
Identity = 41/76 (53.95%), Postives = 56/76 (73.68%), Query Frame = -2 
Query:  182 MQIFVKTLTGKTITLEVES*DTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 409
            M I VKTLTGK I ++++  D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76
The following BLAST results are available for this feature:
BLAST of CN184837 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98
Match NameE-valueIdentityDescription
NEDD8_CAEEL1.168e-1960.53NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ARATH1.526e-1961.33NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
RUB3_ORYSJ1.291e-1853.85NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
NEDD8_RAT1.687e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.687e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.687e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.687e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.687e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ARATH1.428e-1755.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI1.865e-1753.25NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006D07_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006D07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184837 ID=CN184837; Name=CN184837; organism=Citrus sinensis; type=EST; length=773bp
GAGGAACTACTGCTTATATTATTATTGCTGATAGAAAGAGGAAATTAGGC
CCAAGCGGCTGGCCATTACACAATTGTAATTACAAAATAATTAAAGCAAA
GCGCCCAATCCAACCTAATTAATTATTATTAGCGCCATCCAATCAAACGC
GGACACATCCACATATATTTTTTTAGAAGTCACCACCACGGAGACGGAGG
ACAAGGTGGAGAGTGGACTCCTTTTGAATGTTGTAATCGGCGAGAGTCCG
GCCATCCTCGAGCTGCTTGCCGGCGAAGATGAGCCTCTGCTGGTCCGGAG
GAATCCCCTCTTTGTCCTGGATCTTGGCCTTCACGTTGTCGATCGTGTCT
TAGCTCTCCACCTCCAGGGTAATCGTCTTCCCGGTCAACGTCTTGACGAA
GATCTGCATTCCTCCCCTCAGGCGCAACACAAGGTGGAGCGTGGATTCCT
TCTGGATGTTATAATCGGCCAGGGTGCGGCCGTCCTCCAGCTGCTTGCCG
GCGAAGATGAGCCTCTGCTGGTCCGGGGGGATCCCTTCCTTGTCCTGGAT
CTTCGCCTTCACGTTGTCGATCGTGTCGGAGCTCTCCACCTCCAGGGTGA
TCGTCTTCCCGGTCAGAGTCTTGACGAAGATCTGCATGCCGCCGCGGAGA
CGGAGGACGAGGTGGAGAGTCGACTCCTTCTGGATGTTGTAATCCGCCAA
GGTGCGCCCGTCCTCGAGCTGCTTTCCGGCGAAGATCAGCCGCTGCTGGT
CCGGTGGAATCCCTTCCTTGTCC
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