CN184828

Overview
NameCN184828
Unique NameCN184828
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length338
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10200 contig Csv1_Contig10200:153..489. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS2_TULGE (Sucrose synthase 2 OS=Tulipa gesneriana PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 9.082e-29
Identity = 50/84 (59.52%), Postives = 68/84 (80.95%), Query Frame = 1 
Query:    4 MAERALTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFES-ISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            MA+RA+TRVHS+RERL +TLSAH+NE+LAL SR   +G+G+LQ HQL+ E+E+ I   +R+ L +G F +VL+A QEAIV+PPW
Sbjct:    1 MADRAMTRVHSVRERLTDTLSAHKNELLALFSRFVKQGQGMLQPHQLLTEYEAVIPAADREKLKDGVFEDVLKAAQEAIVIPPW 84

HSP 2 Score: 43.1282 bits (100), Expect = 9.082e-29
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVWEY+RVNV  L
Sbjct:   85 VALAIRPRPGVWEYVRVNVSEL 106
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS1_TULGE (Sucrose synthase 1 OS=Tulipa gesneriana PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 7.495e-28
Identity = 48/84 (57.14%), Postives = 65/84 (77.38%), Query Frame = 1 
Query:    4 MAERALTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFES-ISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            M ER LTR+HS++ERL ++LS H NE+LAL SR   +GKG+L+ HQL+ E+ES I E +R+ L +G F + LRA+QEAIV+PPW
Sbjct:    1 MTERRLTRIHSIKERLGDSLSHHPNELLALFSRFIKQGKGMLERHQLLTEYESVIPEADREKLKDGVFEDTLRASQEAIVIPPW 84

HSP 2 Score: 43.8986 bits (102), Expect = 7.495e-28
Identity = 18/22 (81.82%), Postives = 21/22 (95.45%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVWEY+RVNV+ L
Sbjct:   85 VALAIRPRPGVWEYVRVNVNEL 106
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS2_MAIZE (Sucrose synthase 2 OS=Zea mays GN=SUS1 PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.047e-26
Identity = 45/82 (54.88%), Postives = 66/82 (80.49%), Query Frame = 1 
Query:   10 ERALTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEF-ESISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            +R L+R+HS+RER+ ++LSAH NE++A+ +R++  GKG+LQ HQ+IAE+  +I E  R+ L +GAF +VLRA QEAIV+PPW
Sbjct:    7 DRVLSRLHSVRERIGDSLSAHPNELVAVFTRLKNLGKGMLQPHQIIAEYNNAIPEAEREKLKDGAFEDVLRAAQEAIVIPPW 88

HSP 2 Score: 43.1282 bits (100), Expect = 1.047e-26
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVWEY+RVNV  L
Sbjct:   89 VALAIRPRPGVWEYVRVNVSEL 110
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS3_ORYSJ (Sucrose synthase 3 OS=Oryza sativa subsp. japonica GN=SUS3 PE=1 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 3.005e-26
Identity = 46/82 (56.10%), Postives = 64/82 (78.05%), Query Frame = 1 
Query:   10 ERALTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFE-SISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            ERALTR+HS+RER+ ++LSAH NE++A+ SR+  +GKG+LQ HQ+IAE+  +I E  R+ L + A  +VLR  QEAIV+PPW
Sbjct:    7 ERALTRLHSMRERIGDSLSAHTNELVAVFSRLVNQGKGMLQPHQIIAEYNAAIPEGEREKLKDSALEDVLRGAQEAIVIPPW 88

HSP 2 Score: 41.9726 bits (97), Expect = 3.005e-26
Identity = 16/22 (72.73%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            +ALA+RPRPGVWEY+R+NV  L
Sbjct:   89 IALAIRPRPGVWEYLRINVSQL 110
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS1_ORYSJ (Sucrose synthase 1 OS=Oryza sativa subsp. japonica GN=SUS1 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.474e-25
Identity = 43/82 (52.44%), Postives = 65/82 (79.27%), Query Frame = 1 
Query:   10 ERALTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEF-ESISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            +R L+R+HS+RER+ ++LSAH NE++A+ +R+   GKG+LQ HQ+IAE+  +ISE +R+ L +GAF +VLR+ QE IV+ PW
Sbjct:    7 DRVLSRLHSVRERIGDSLSAHPNELVAVFTRLVNLGKGMLQAHQIIAEYNNAISEADREKLKDGAFEDVLRSAQEGIVISPW 88

HSP 2 Score: 43.1282 bits (100), Expect = 2.474e-25
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVWEY+RVNV  L
Sbjct:   89 VALAIRPRPGVWEYVRVNVSEL 110
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS2_HORVU (Sucrose synthase 2 OS=Hordeum vulgare GN=SS2 PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.453e-25
Identity = 44/82 (53.66%), Postives = 61/82 (74.39%), Query Frame = 1 
Query:   10 ERALTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFE-SISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            ERAL+RVHS+RER+  +LSAH NE++A+ SR+  +GKG+LQ HQ+ AE+  +I E  R+ L    F ++LR  QEAIV+PPW
Sbjct:    7 ERALSRVHSVRERIGHSLSAHTNELVAVFSRLVNQGKGMLQPHQITAEYNAAIPEAEREKLKNTPFEDLLRGAQEAIVIPPW 88

HSP 2 Score: 43.1282 bits (100), Expect = 5.453e-25
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVWEY+RVNV  L
Sbjct:   89 VALAIRPRPGVWEYVRVNVSEL 110
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS1_MAIZE (Sucrose synthase 1 OS=Zea mays GN=SH-1 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.649e-24
Identity = 43/78 (55.13%), Postives = 59/78 (75.64%), Query Frame = 1 
Query:   19 LTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFESISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            LTR+HSLRERL  T S+H NE++AL SR   +GKG+LQ HQL+AEF+++ + +++      F ++LRA QEAIVLPPW
Sbjct:    5 LTRLHSLRERLGATFSSHPNELIALFSRYVHQGKGMLQRHQLLAEFDALFDSDKEKY--APFEDILRAAQEAIVLPPW 80

HSP 2 Score: 42.3578 bits (98), Expect = 2.649e-24
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVW+YIRVNV  L
Sbjct:   81 VALAIRPRPGVWDYIRVNVSEL 102
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS2_ORYSJ (Sucrose synthase 2 OS=Oryza sativa subsp. japonica GN=SUS2 PE=1 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 3.447e-24
Identity = 43/78 (55.13%), Postives = 58/78 (74.36%), Query Frame = 1 
Query:   19 LTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFESISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            L R+HSLRERL  T S+H NE++AL SR   +GKG+LQ HQL+AEF+++ E +++      F ++LRA QEAIVLPPW
Sbjct:    5 LARLHSLRERLGATFSSHPNELIALFSRYVNQGKGMLQRHQLLAEFDALIEADKEKY--APFEDILRAAQEAIVLPPW 80

HSP 2 Score: 42.3578 bits (98), Expect = 3.447e-24
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPRPGVW+YIRVNV  L
Sbjct:   81 VALAIRPRPGVWDYIRVNVSEL 102
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS1_HORVU (Sucrose synthase 1 OS=Hordeum vulgare GN=SS1 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 9.884e-24
Identity = 44/78 (56.41%), Postives = 59/78 (75.64%), Query Frame = 1 
Query:   19 LTRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFESISEENRKHLTEGAFGEVLRATQEAIVLPPW 252
            LTR+HSLRERL  T S+H NE++AL SR   +GKG+LQ HQL+AEF+++ E +++      F ++LRA QEAIVLPPW
Sbjct:    5 LTRLHSLRERLGATFSSHPNELIALFSRYVHQGKGMLQRHQLLAEFDALFESDKEKY--APFEDILRAAQEAIVLPPW 80

HSP 2 Score: 39.2762 bits (90), Expect = 9.884e-24
Identity = 17/22 (77.27%), Postives = 19/22 (86.36%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VALA+RPR GVW+YIRVNV  L
Sbjct:   81 VALAIRPRTGVWDYIRVNVSEL 102
[Close]
BLAST of CN184828 vs. ExPASy Swiss-Prot
Match: SUS2_PEA (Sucrose synthase 2 OS=Pisum sativum GN=SUS2 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.056e-22
Identity = 42/79 (53.16%), Postives = 58/79 (73.42%), Query Frame = 1 
Query:   22 TRVHSLRERLDETLSAHRNEILALLSRIEGKGKGILQNHQLIAEFESISEENRK--HLTEGAFGEVLRATQEAIVLPPW 252
            TRV S+R+R+ +TLSAHRNE+++LLSR   +GKGILQ H LI E ++I  E+     L  G FG+++ + QEAIVLPP+
Sbjct:    8 TRVPSIRDRVQDTLSAHRNELISLLSRYVAQGKGILQPHNLIDELDNILGEDHATLDLKNGPFGQIINSAQEAIVLPPF 86

HSP 2 Score: 42.743 bits (99), Expect = 1.056e-22
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2 
Query:  254 VALAVRPRPGVWEYIRVNVHAL 319
            VA+AVRPRPGVWEY+RVNV  L
Sbjct:   87 VAIAVRPRPGVWEYVRVNVFEL 108
The following BLAST results are available for this feature:
BLAST of CN184828 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 21
Match NameE-valueIdentityDescription
SUS2_TULGE9.082e-2959.52Sucrose synthase 2 OS=Tulipa gesneriana PE=2 SV=1[more]
SUS1_TULGE7.495e-2857.14Sucrose synthase 1 OS=Tulipa gesneriana PE=2 SV=1[more]
SUS2_MAIZE1.047e-2654.88Sucrose synthase 2 OS=Zea mays GN=SUS1 PE=1 SV=1[more]
SUS3_ORYSJ3.005e-2656.10Sucrose synthase 3 OS=Oryza sativa subsp. japonica... [more]
SUS1_ORYSJ2.474e-2552.44Sucrose synthase 1 OS=Oryza sativa subsp. japonica... [more]
SUS2_HORVU5.453e-2553.66Sucrose synthase 2 OS=Hordeum vulgare GN=SS2 PE=1 ... [more]
SUS1_MAIZE2.649e-2455.13Sucrose synthase 1 OS=Zea mays GN=SH-1 PE=2 SV=1[more]
SUS2_ORYSJ3.447e-2455.13Sucrose synthase 2 OS=Oryza sativa subsp. japonica... [more]
SUS1_HORVU9.884e-2456.41Sucrose synthase 1 OS=Hordeum vulgare GN=SS1 PE=1 ... [more]
SUS2_PEA1.056e-2253.16Sucrose synthase 2 OS=Pisum sativum GN=SUS2 PE=2 S... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006D02_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006D02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184828 ID=CN184828; Name=CN184828; organism=Citrus sinensis; type=EST; length=338bp
TCAATGGCCGAACGTGCTTTGACCCGCGTTCACAGTCTCCGCGAGCGTTT
GGATGAGACTCTCTCTGCCCACAGGAATGAAATCTTGGCCCTTTTGTCAA
GGATTGAGGGCAAGGGCAAGGGAATCTTGCAAAACCATCAACTTATTGCT
GAGTTTGAATCAATTTCTGAGGAAAACAGAAAGCATCTAACTGAGGGGGC
ATTTGGTGAAGTTCTAAGAGCCACCCAGGAAGCAATTGTTTTGCCTCCGT
GGGGTAGCGCTTGCCGTGCGTCCAAGGCCCGGTGTGTGGGAGTACATCCG
AGTGAATGTCCATGCCCTTGTCCGTTGAAGGAGCTGCT
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