CN184817

Overview
NameCN184817
Unique NameCN184817
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length734
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_HELHP (Serine hydroxymethyltransferase OS=Helicobacter hepaticus GN=glyA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.725e-31
Identity = 75/182 (41.21%), Postives = 109/182 (59.89%), Query Frame = -3 
Query:  130 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVY 672
            KIN+AVFPG+QGGP  H I+G AV  K+   PE+K Y +QV SN    A+ L++R Y+LVSGG++NHL+L++  NK   G   +  L +  I  NKNT+PG++ S  +  GIR+G+PALT+RG  E++FE + E      K+A  I  D   T L+         E I++++ N      +Y
Sbjct:  246 KINKAVFPGIQGGPLMHIIAGKAVGFKENLKPEWKIYAKQVKSNIQALAEVLIKRNYELVSGGSDNHLILMSFLNKDFSGKDADLALGNAGITVNKNTIPGEIRSPFITSGIRIGSPALTARGMKEKEFEWIGE------KIA-DILDDINNTNLQ---------EHIKAQVKNFSRDFRIY 411
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOPS (Serine hydroxymethyltransferase OS=Clostridium perfringens (strain SM101 / Type A) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.725e-31
Identity = 65/140 (46.43%), Postives = 94/140 (67.14%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++ +FPG+QGGP  H I+  AV  K+   P +K Y EQV+ N+   A++L   G+ LVS GT+NHL+LV+L NK I G   E +L+S+ I  NKNTVP +  S  V  G+R+GTPA+T+RGF EE+ +++A   N A+K
Sbjct:  248 LDKNIFPGIQGGPLMHIIAAKAVCFKEALEPSFKTYMEQVVKNAHVLAEALESYGFKLVSNGTDNHLILVDLTNKDITGKDAEILLDSIGITLNKNTVPNETRSPFVTSGVRIGTPAITTRGFKEEEMKEIASIINDAIK 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOP1 (Serine hydroxymethyltransferase OS=Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.725e-31
Identity = 65/140 (46.43%), Postives = 94/140 (67.14%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++ +FPG+QGGP  H I+  AV  K+   P +K Y EQV+ N+   A++L   G+ LVS GT+NHL+LV+L NK I G   E +L+S+ I  NKNTVP +  S  V  G+R+GTPA+T+RGF EE+ +++A   N A+K
Sbjct:  248 LDKNIFPGIQGGPLMHIIAAKAVCFKEALEPSFKTYMEQVVKNAHVLAEALESYGFKLVSNGTDNHLILVDLTNKDITGKDAEILLDSIGITLNKNTVPNETRSPFVTSGVRIGTPAITTRGFKEEEMKEIASIINDAIK 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB8 (Serine hydroxymethyltransferase OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.725e-31
Identity = 64/140 (45.71%), Postives = 93/140 (66.43%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +N+ +FPG+QGGP  H I+  AV  K+   P +K Y E V+ N  + A+ L+ RG+ +VSGGT+NH+ LV+L NK I G   E +L+SV I  NKNTVP +  S  V  GIR+GT A+T+RGFV++D  ++A   + A++
Sbjct:  248 LNKNIFPGIQGGPLEHIIAAKAVCFKEALDPSFKTYGENVVENCKELAEQLIARGFKIVSGGTDNHVFLVDLNNKDITGKEAEALLDSVGITVNKNTVPNETRSPFVTSGIRIGTAAITTRGFVKDDMAEIAAVISEAIE 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CAMHC (Serine hydroxymethyltransferase OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.725e-31
Identity = 77/184 (41.85%), Postives = 106/184 (57.61%), Query Frame = -3 
Query:  124 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAK 672
            KIN ++FPG+QGGP  H I+G AV  K   +PE+K Y +QV +N      +L++RG+DLVSGGT+NHL+LV+   K   G      LE+  I  NKNTVPG+  S  V  GIR+G+ ALTSRG  E++FE +A       K+A  +  D   T L+           I++E+  L  K  +Y K
Sbjct:  245 KINSSIFPGMQGGPLVHVIAGKAVGFKHNLSPEWKTYAKQVKANCKILGDTLMKRGFDLVSGGTDNHLILVSFLKKDYSGKDASNALENAGITVNKNTVPGETRSPFVTSGIRVGSAALTSRGMKEKEFEWIAN------KIA-DVLNDINNTSLQ---------SKIKAEVKELASKFIIYDK 412
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA2_PSEPF (Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=glyA2 PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.725e-31
Identity = 72/193 (37.31%), Postives = 109/193 (56.48%), Query Frame = -3 
Query:  106 MKTKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFPTVG 681
            ++ K+N AVFPG QGGP  H I+  A+  K+   PE+KAYQ+QV+ N+   A   +ERG+D+VSGGTENHL L++L  + I G   +  L    I  NKN+VP D  S  V  G+R GTPA+T+RGF E + +++A                        ++  +++D + ++ I  +R+KV+   K+ P  G
Sbjct:  247 IEKKLNSAVFPGAQGGPLEHVIAAKAICFKEALQPEFKAYQQQVVKNAQAMAGVFIERGFDVVSGGTENHLFLLSLIKQEISGKDADAALGKAFITVNKNSVPNDPRSPFVTSGLRFGTPAVTTRGFKEAECKELA-----------------------GWICDILADLNNEAVIDAVREKVKAICKKLPVYG 416
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_SHISS (Serine hydroxymethyltransferase OS=Shigella sonnei (strain Ss046) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.558e-31
Identity = 68/133 (51.13%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  277 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVA 672
            K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S  V  GIR+GTPA+T RGF E + +++A
Sbjct:  251 KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELA 383
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_SHIFL (Serine hydroxymethyltransferase OS=Shigella flexneri GN=glyA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.558e-31
Identity = 68/133 (51.13%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  277 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVA 672
            K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S  V  GIR+GTPA+T RGF E + +++A
Sbjct:  251 KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELA 383
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_SHIF8 (Serine hydroxymethyltransferase OS=Shigella flexneri serotype 5b (strain 8401) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.558e-31
Identity = 68/133 (51.13%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  277 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVA 672
            K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S  V  GIR+GTPA+T RGF E + +++A
Sbjct:  251 KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELA 383
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_SHIDS (Serine hydroxymethyltransferase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=glyA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.558e-31
Identity = 68/133 (51.13%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  277 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVA 672
            K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S  V  GIR+GTPA+T RGF E + +++A
Sbjct:  251 KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELA 383
The following BLAST results are available for this feature:
BLAST of CN184817 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_HELHP2.725e-3141.21Serine hydroxymethyltransferase OS=Helicobacter he... [more]
GLYA_CLOPS2.725e-3146.43Serine hydroxymethyltransferase OS=Clostridium per... [more]
GLYA_CLOP12.725e-3146.43Serine hydroxymethyltransferase OS=Clostridium per... [more]
GLYA_CLOB82.725e-3145.71Serine hydroxymethyltransferase OS=Clostridium bei... [more]
GLYA_CAMHC2.725e-3141.85Serine hydroxymethyltransferase OS=Campylobacter h... [more]
GLYA2_PSEPF2.725e-3137.31Serine hydroxymethyltransferase 2 OS=Pseudomonas f... [more]
GLYA_SHISS3.558e-3151.13Serine hydroxymethyltransferase OS=Shigella sonnei... [more]
GLYA_SHIFL3.558e-3151.13Serine hydroxymethyltransferase OS=Shigella flexne... [more]
GLYA_SHIF83.558e-3151.13Serine hydroxymethyltransferase OS=Shigella flexne... [more]
GLYA_SHIDS3.558e-3151.13Serine hydroxymethyltransferase OS=Shigella dysent... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006C16_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006C16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184817 ID=CN184817; Name=CN184817; organism=Citrus sinensis; type=EST; length=734bp
GAGGCTATTTTATATGCTTTCAAGAAACAAATATATGACATACCTTGCTG
CATAAATATCATTAATCATCCTTCAGTTCTTGTATTTCATTGTTTCTTTC
TCAAAACCAACAGTAGGAAATCGTTTTGCGTACACCTCGACTTTGTCTCG
GAGGTTTGAAATCTCAGATTGAATGCTTTCATCTGACATCAAAGTTGCTA
CAAAATCCTTCAACTTTGTTCCTTTAGTATCACCCTTGATCTTTAATGCC
AACTTCACAGCTGCATTGAAGAACTCAGCGACCTTTTCAAAATCTTCCTC
AACAAACCCCCTAGATGTGAGGGCTGGGGTTCCCATGCGGATGCCACCAG
GAACCATAGCAGACACGTCACCAGGAACAGTGTTTTTGTTGGCTGCAATA
TGAACTGATTCCAAAACCTTTTCAACTCTTGATCCGTCAATACCCTTGTT
TCTCAAGTTCACCAAGACTAGATGGTTCTCAGTTCCACCAGATACCAGAT
CATAACCTCTCTCTAGCAAACTCTGTGCAAACTTTGAAGAATTGCTGAGA
ACTTGCTCCTGGTAGGCCTTGTATTCAGGGGTCTTTACCTGCTTAAGTGC
GACAGCTAATCCTGATATGGTGTGATTATGTGGACCACCTTGAAGTCCAG
GGAAAACTGCCTGATTAATTTTTGTCTTCATAGGGCATACATCACTTCTT
GTCCTTGTTTATTAATCTCTTTAACCCCCTTCCT
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