CN184817

Overview
NameCN184817
Unique NameCN184817
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length734
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_RICRO (Serine hydroxymethyltransferase OS=Rickettsia rickettsii (strain Iowa) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 69/129 (53.49%), Postives = 88/129 (68.22%), Query Frame = -3 
Query:  289 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDF 672
            KIN A+FPGLQGGP  H I+  AVA  +   PEYK+Y +QV+SN+   A SL ERGYD+++GGT+NH+VLV+LR  GI G      L+   I  NKN +P D  S  +  GIR+GTPA T+RGF E+DF
Sbjct:  250 KINSALFPGLQGGPLMHIIAAKAVAFLENLQPEYKSYIQQVISNAKALASSLQERGYDILTGGTDNHIVLVDLRKDGITGKLAANSLDRAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEKDF 378
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_RICPU (Serine hydroxymethyltransferase OS=Rickettsia peacockii (strain Rustic) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 69/129 (53.49%), Postives = 88/129 (68.22%), Query Frame = -3 
Query:  289 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDF 672
            KIN A+FPGLQGGP  H I+  AVA  +   PEYK+Y +QV+SN+   A SL ERGYD+++GGT+NH+VLV+LR  GI G      L+   I  NKN +P D  S  +  GIR+GTPA T+RGF E+DF
Sbjct:  250 KINSALFPGLQGGPLMHIIAAKAVAFLENLQPEYKSYIQQVISNAKALASSLQERGYDILTGGTDNHIVLVDLRKDGITGKLAANSLDRAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEKDF 378
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_RICCN (Serine hydroxymethyltransferase OS=Rickettsia conorii GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 69/129 (53.49%), Postives = 88/129 (68.22%), Query Frame = -3 
Query:  289 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDF 672
            KIN A+FPGLQGGP  H I+  AVA  +   PEYK+Y +QV+SN+   A SL ERGYD+++GGT+NH+VLV+LR  GI G      L+   I  NKN +P D  S  +  GIR+GTPA T+RGF E+DF
Sbjct:  250 KINSALFPGLQGGPLMHIIAAKAVAFLENLQPEYKSYIQQVISNAKALASSLQERGYDILTGGTDNHIVLVDLRKDGITGKLAANSLDRAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEKDF 378
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_RICAE (Serine hydroxymethyltransferase OS=Rickettsia africae (strain ESF-5) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 69/129 (53.49%), Postives = 88/129 (68.22%), Query Frame = -3 
Query:  289 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDF 672
            KIN A+FPGLQGGP  H I+  AVA  +   PEYK+Y +QV+SN+   A SL ERGYD+++GGT+NH+VLV+LR  GI G      L+   I  NKN +P D  S  +  GIR+GTPA T+RGF E+DF
Sbjct:  250 KINSALFPGLQGGPLMHIIAAKAVAFLENLQPEYKSYIQQVISNAKALASSLQERGYDILTGGTDNHIVLVDLRKDGITGKLAANSLDRAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEKDF 378
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_PSEU5 (Serine hydroxymethyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 73/193 (37.82%), Postives = 108/193 (55.96%), Query Frame = -3 
Query:  106 MKTKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFPTVG 681
            ++ K+N AVFPG QGGP  H I+  AV  K+   PE+KAYQ+QV+ N+   A+  ++RG+D+VSGGT+NHL L++L  + I G   +  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RGF                       G+ +  +L  ++  ++ +   +S I  +R KVE    +FP  G
Sbjct:  247 IEKKLNSAVFPGAQGGPLEHVIAAKAVCFKEALQPEFKAYQQQVVKNAQAMAEVFIQRGFDVVSGGTQNHLFLLSLIKQDITGKDADAALGRAHITVNKNSVPNDPRSPFVTSGLRIGTPAVTTRGF-----------------------GEAECRELAGWICDILDNMGDESVIDAVRGKVEAVCAKFPVYG 416
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_PHYAS (Serine hydroxymethyltransferase OS=Phytoplasma australiense GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 67/143 (46.85%), Postives = 100/143 (69.93%), Query Frame = -3 
Query:  253 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLR--NKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 672
            +IN++VFPG+QGGP  H I+  AV+ K+ ++ E+K YQ+QV+ N+  FAQ+  ++GY +VS GT+NHL L+NL+  N    G ++  +LE V+I  NKNT+P D    M   GIR+GTPA+T++GF E DF K+A+  + A+K
Sbjct:  248 QINRSVFPGIQGGPLMHVIAAKAVSFKEAQSLEFKNYQQQVIKNAQAFAQTFQKKGYHVVSQGTDNHLFLINLKKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSGIRLGTPAMTTKGFQEADFIKLADLIDQAIK 390
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_LEGPH (Serine hydroxymethyltransferase OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 74/190 (38.95%), Postives = 109/190 (57.37%), Query Frame = -3 
Query:  115 MKTKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFP 681
            ++ K+N +VFPG+QGGP  H I+  AVA  +   PE+K YQ+QVL+N+      L  RGYD+VSGGT+NHL+LV+L NKGI G   +  +   +I  NKN+VP D  S  V  G+R+GTPA T+RGF E +                        T L ++VA ++ +   ++ IS ++ +V +  + FP
Sbjct:  248 IEKKLNSSVFPGMQGGPLMHVIAAKAVAFAEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEADAAVGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREI-----------------------TLLSNWVADVLDNVHDETNISRVKTQVLLLCREFP 414
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_LEGPA (Serine hydroxymethyltransferase OS=Legionella pneumophila (strain Paris) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 74/190 (38.95%), Postives = 109/190 (57.37%), Query Frame = -3 
Query:  115 MKTKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFP 681
            ++ K+N +VFPG+QGGP  H I+  AVA  +   PE+K YQ+QVL+N+      L  RGYD+VSGGT+NHL+LV+L NKGI G   +  +   +I  NKN+VP D  S  V  G+R+GTPA T+RGF E +                        T L ++VA ++ +   ++ IS ++ +V +  + FP
Sbjct:  248 IEKKLNSSVFPGMQGGPLMHVIAAKAVAFAEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEADAAVGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREI-----------------------TLLSNWVADVLDNVHDETNISRVKTQVLLLCREFP 414
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBM (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 64/140 (45.71%), Postives = 97/140 (69.29%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++A+FPG+QGGP  HTI+  AV   +    +YK Y +QV+ N+    + L   G+ L+SGGT+NHL+L++L NK I G   EK+L+SV I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+ +K+A F N +++
Sbjct:  248 VDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBK (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Okra / Type B1) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 64/140 (45.71%), Postives = 97/140 (69.29%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++A+FPG+QGGP  HTI+  AV   +    +YK Y +QV+ N+    + L   G+ L+SGGT+NHL+L++L NK I G   EK+L+SV I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+ +K+A F N +++
Sbjct:  248 VDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387
The following BLAST results are available for this feature:
BLAST of CN184817 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_RICRO5.490e-3253.49Serine hydroxymethyltransferase OS=Rickettsia rick... [more]
GLYA_RICPU5.490e-3253.49Serine hydroxymethyltransferase OS=Rickettsia peac... [more]
GLYA_RICCN5.490e-3253.49Serine hydroxymethyltransferase OS=Rickettsia cono... [more]
GLYA_RICAE5.490e-3253.49Serine hydroxymethyltransferase OS=Rickettsia afri... [more]
GLYA_PSEU55.490e-3237.82Serine hydroxymethyltransferase OS=Pseudomonas stu... [more]
GLYA_PHYAS5.490e-3246.85Serine hydroxymethyltransferase OS=Phytoplasma aus... [more]
GLYA_LEGPH5.490e-3238.95Serine hydroxymethyltransferase OS=Legionella pneu... [more]
GLYA_LEGPA5.490e-3238.95Serine hydroxymethyltransferase OS=Legionella pneu... [more]
GLYA_CLOBM5.490e-3245.71Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOBK5.490e-3245.71Serine hydroxymethyltransferase OS=Clostridium bot... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006C16_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006C16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184817 ID=CN184817; Name=CN184817; organism=Citrus sinensis; type=EST; length=734bp
GAGGCTATTTTATATGCTTTCAAGAAACAAATATATGACATACCTTGCTG
CATAAATATCATTAATCATCCTTCAGTTCTTGTATTTCATTGTTTCTTTC
TCAAAACCAACAGTAGGAAATCGTTTTGCGTACACCTCGACTTTGTCTCG
GAGGTTTGAAATCTCAGATTGAATGCTTTCATCTGACATCAAAGTTGCTA
CAAAATCCTTCAACTTTGTTCCTTTAGTATCACCCTTGATCTTTAATGCC
AACTTCACAGCTGCATTGAAGAACTCAGCGACCTTTTCAAAATCTTCCTC
AACAAACCCCCTAGATGTGAGGGCTGGGGTTCCCATGCGGATGCCACCAG
GAACCATAGCAGACACGTCACCAGGAACAGTGTTTTTGTTGGCTGCAATA
TGAACTGATTCCAAAACCTTTTCAACTCTTGATCCGTCAATACCCTTGTT
TCTCAAGTTCACCAAGACTAGATGGTTCTCAGTTCCACCAGATACCAGAT
CATAACCTCTCTCTAGCAAACTCTGTGCAAACTTTGAAGAATTGCTGAGA
ACTTGCTCCTGGTAGGCCTTGTATTCAGGGGTCTTTACCTGCTTAAGTGC
GACAGCTAATCCTGATATGGTGTGATTATGTGGACCACCTTGAAGTCCAG
GGAAAACTGCCTGATTAATTTTTGTCTTCATAGGGCATACATCACTTCTT
GTCCTTGTTTATTAATCTCTTTAACCCCCTTCCT
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