CN184817

Overview
NameCN184817
Unique NameCN184817
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length734
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBJ (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Kyoto / Type A2) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 64/140 (45.71%), Postives = 97/140 (69.29%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++A+FPG+QGGP  HTI+  AV   +    +YK Y +QV+ N+    + L   G+ L+SGGT+NHL+L++L NK I G   EK+L+SV I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+ +K+A F N +++
Sbjct:  248 VDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBH (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 64/140 (45.71%), Postives = 97/140 (69.29%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++A+FPG+QGGP  HTI+  AV   +    +YK Y +QV+ N+    + L   G+ L+SGGT+NHL+L++L NK I G   EK+L+SV I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+ +K+A F N +++
Sbjct:  248 VDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB6 (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain 657 / Type Ba4) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 64/140 (45.71%), Postives = 97/140 (69.29%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++A+FPG+QGGP  HTI+  AV   +    +YK Y +QV+ N+    + L   G+ L+SGGT+NHL+L++L NK I G   EK+L+SV I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+ +K+A F N +++
Sbjct:  248 VDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB1 (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain ATCC 19397 / Type A) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 64/140 (45.71%), Postives = 97/140 (69.29%), Query Frame = -3 
Query:  253 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVK 669
            +++A+FPG+QGGP  HTI+  AV   +    +YK Y +QV+ N+    + L   G+ L+SGGT+NHL+L++L NK I G   EK+L+SV I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+ +K+A F N +++
Sbjct:  248 VDKAIFPGIQGGPLMHTIAAKAVCFGEALREDYKEYMQQVVKNTKVLGEELKNYGFRLISGGTDNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_ACIRA (Serine hydroxymethyltransferase OS=Acinetobacter radioresistens GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.490e-32
Identity = 73/190 (38.42%), Postives = 109/190 (57.37%), Query Frame = -3 
Query:  115 MKTKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFP 681
            ++ K+  AVFPG QGGP  H ++  A+  K+   PEYKAYQ+QV+ N+   A+ L+ERGYD+VSGGT+NHL L++L  + I G   +  L + HI  NKN+VP D  S  V  GIR+GTPA+T+RGF                       G+ +   L  ++A ++  +  ++ I+ ++ KVE    +FP
Sbjct:  247 IEKKLQSAVFPGNQGGPLVHAVAAKAICFKEAMAPEYKAYQQQVVKNAQAMAEVLIERGYDVVSGGTKNHLFLLSLIKQDITGKDADAWLGAAHITVNKNSVPNDPRSPFVTSGIRIGTPAVTTRGF-----------------------GEAEVRDLASWIADILDSKGDEAVINTVKAKVEAVCAKFP 413
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_XANCP (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. campestris GN=glyA PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.170e-32
Identity = 71/190 (37.37%), Postives = 106/190 (55.79%), Query Frame = -3 
Query:  106 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFPTVG 672
            K+   VFPG+QGGP  H I+  AVA K+   PE+K YQ+QV+ N+   A +L+ RGY +VSGGTENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D                          L +++A ++   +  + ++ +RD V    K++P  G
Sbjct:  251 KLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPEFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDC-----------------------VDLANWIADVLDAPADDAVLAKVRDAVTAQCKKYPVYG 417
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_XANCB (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. campestris (strain B100) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.170e-32
Identity = 71/190 (37.37%), Postives = 106/190 (55.79%), Query Frame = -3 
Query:  106 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFPTVG 672
            K+   VFPG+QGGP  H I+  AVA K+   PE+K YQ+QV+ N+   A +L+ RGY +VSGGTENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D                          L +++A ++   +  + ++ +RD V    K++P  G
Sbjct:  251 KLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPEFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDC-----------------------VDLANWIADVLDAPADDAVLAKVRDAVTAQCKKYPVYG 417
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_XANC8 (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. campestris (strain 8004) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.170e-32
Identity = 71/190 (37.37%), Postives = 106/190 (55.79%), Query Frame = -3 
Query:  106 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMSDESIQSEISNLRDKVEVYAKRFPTVG 672
            K+   VFPG+QGGP  H I+  AVA K+   PE+K YQ+QV+ N+   A +L+ RGY +VSGGTENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D                          L +++A ++   +  + ++ +RD V    K++P  G
Sbjct:  251 KLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPEFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDC-----------------------VDLANWIADVLDAPADDAVLAKVRDAVTAQCKKYPVYG 417
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_THEP1 (Serine hydroxymethyltransferase OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.170e-32
Identity = 71/152 (46.71%), Postives = 96/152 (63.16%), Query Frame = -3 
Query:  217 INQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGT 669
            +++ +FPG+QGGP  H I+  AV  K+  T E+K YQ+QV+ N+ K A+   +RGY +VSGGT+ HL LV+L  K I G   EK LES  I  NKNT+P +  S  V  GIR+GTPA+T+RG  EE+ E++AE     + L L    D  GT
Sbjct:  248 VDKTIFPGIQGGPLMHVIAAKAVCFKEAMTEEFKEYQKQVVKNAKKMAEEFQKRGYRIVSGGTDTHLFLVDLTPKDITGKAAEKALESCGITVNKNTIPNEKRSPFVASGIRIGTPAVTTRGMKEEEMEEIAEM----IDLVLSNVTDENGT 395
[Close]
BLAST of CN184817 vs. ExPASy Swiss-Prot
Match: GLYA_STACT (Serine hydroxymethyltransferase OS=Staphylococcus carnosus (strain TM300) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.170e-32
Identity = 73/165 (44.24%), Postives = 104/165 (63.03%), Query Frame = -3 
Query:  184 KINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNK-GIDGSRVEKVLESVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFEKVAEFFNAAVKLALKIKGDTKGTKLKDFVATLMS 672
            +I++ +FPG+QGGP  H I+  AVA  +   P++K YQEQV+ N+   A +L+E G+ +VSGGT+NHLV V+++   GI G   E+ L+ V I  NKNT+P D     V  G+R+GTPA T+RGF E DFE+VA+  +  V+     K + K  +  D VA L S
Sbjct:  245 QIDKTIFPGIQGGPLEHVIAAKAVAFGEALNPDFKDYQEQVVKNAKALADTLIEEGFRVVSGGTDNHLVAVDVKGSFGITGKEAEEALDEVGITCNKNTIPFDQEKPFVTSGLRLGTPAATTRGFEEADFEEVAKIISLVVQ---NPKDEAKLKEASDRVAALTS 406
The following BLAST results are available for this feature:
BLAST of CN184817 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_CLOBJ5.490e-3245.71Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOBH5.490e-3245.71Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOB65.490e-3245.71Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOB15.490e-3245.71Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_ACIRA5.490e-3238.42Serine hydroxymethyltransferase OS=Acinetobacter r... [more]
GLYA_XANCP7.170e-3237.37Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]
GLYA_XANCB7.170e-3237.37Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]
GLYA_XANC87.170e-3237.37Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]
GLYA_THEP17.170e-3246.71Serine hydroxymethyltransferase OS=Thermotoga petr... [more]
GLYA_STACT7.170e-3244.24Serine hydroxymethyltransferase OS=Staphylococcus ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006C16_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006C16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184817 ID=CN184817; Name=CN184817; organism=Citrus sinensis; type=EST; length=734bp
GAGGCTATTTTATATGCTTTCAAGAAACAAATATATGACATACCTTGCTG
CATAAATATCATTAATCATCCTTCAGTTCTTGTATTTCATTGTTTCTTTC
TCAAAACCAACAGTAGGAAATCGTTTTGCGTACACCTCGACTTTGTCTCG
GAGGTTTGAAATCTCAGATTGAATGCTTTCATCTGACATCAAAGTTGCTA
CAAAATCCTTCAACTTTGTTCCTTTAGTATCACCCTTGATCTTTAATGCC
AACTTCACAGCTGCATTGAAGAACTCAGCGACCTTTTCAAAATCTTCCTC
AACAAACCCCCTAGATGTGAGGGCTGGGGTTCCCATGCGGATGCCACCAG
GAACCATAGCAGACACGTCACCAGGAACAGTGTTTTTGTTGGCTGCAATA
TGAACTGATTCCAAAACCTTTTCAACTCTTGATCCGTCAATACCCTTGTT
TCTCAAGTTCACCAAGACTAGATGGTTCTCAGTTCCACCAGATACCAGAT
CATAACCTCTCTCTAGCAAACTCTGTGCAAACTTTGAAGAATTGCTGAGA
ACTTGCTCCTGGTAGGCCTTGTATTCAGGGGTCTTTACCTGCTTAAGTGC
GACAGCTAATCCTGATATGGTGTGATTATGTGGACCACCTTGAAGTCCAG
GGAAAACTGCCTGATTAATTTTTGTCTTCATAGGGCATACATCACTTCTT
GTCCTTGTTTATTAATCTCTTTAACCCCCTTCCT
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