CN184725 | Citrus Genome Database

Overview


Name	CN184725
Unique Name	CN184725
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	506

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig459	contig	Csv1_Contig459:565..1070.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.081e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 78.1814 bits (191), Expect = 2.443e-14
Identity = 35/59 (59.32%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:   18 EPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 1.132e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -1

Query:  102 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 323
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76

HSP 2 Score: 79.7221 bits (195), Expect = 8.395e-15
Identity = 37/60 (61.67%), Postives = 47/60 (78.33%), Query Frame = -1

Query:  327 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 506
            E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:   18 EPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.337e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.0998 bits (183), Expect = 2.068e-13
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   18 EPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.337e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.0998 bits (183), Expect = 2.068e-13
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   18 EPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 7.337e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.0998 bits (183), Expect = 2.068e-13
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   18 EPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.337e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.0998 bits (183), Expect = 2.068e-13
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   18 EPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 7.337e-19
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.0998 bits (183), Expect = 2.068e-13
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   18 EPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 9.583e-19
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -1

Query:  102 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 329
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 76.6406 bits (187), Expect = 7.107e-14
Identity = 34/59 (57.63%), Postives = 45/59 (76.27%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:   18 EMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.635e-18
Identity = 43/78 (55.13%), Postives = 58/78 (74.36%), Query Frame = -1

Query:   96 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGEC 329
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG C
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGGCC 78

HSP 2 Score: 72.4034 bits (176), Expect = 1.340e-12
Identity = 32/59 (54.24%), Postives = 44/59 (74.58%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:   18 ELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

BLAST of CN184725 vs. ExPASy Swiss-Prot
Match: UBL1_SCHPO (Ubiquitin-like protein 1 OS=Schizosaccharomyces pombe GN=ubl1 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.135e-18
Identity = 41/78 (52.56%), Postives = 57/78 (73.08%), Query Frame = -1

Query:   96 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGEC 329
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG C
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGGSC 78

HSP 2 Score: 69.3218 bits (168), Expect = 1.135e-11
Identity = 29/59 (49.15%), Postives = 43/59 (72.88%), Query Frame = -1

Query:  330 ESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 506
            + +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:   18 DPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76

The following BLAST results are available for this feature:

BLAST of CN184725 vs. ExPASy Swiss-Prot

BLAST of CN184725 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 97


Match Name	E-value	Identity	Description
NEDD8_CAEEL	5.081e-20	60.53	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB2_ARATH	1.132e-19	62.16	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	7.337e-19	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	7.337e-19	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	7.337e-19	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	7.337e-19	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	7.337e-19	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	9.583e-19	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	1.635e-18	55.13	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
UBL1_SCHPO	2.135e-18	52.56	Ubiquitin-like protein 1 OS=Schizosaccharomyces po...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS04_0005P18_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005P18, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN184725 ID=CN184725; Name=CN184725; organism=Citrus sinensis; type=EST; length=506bp
GAGGGTCATGAATTAGTTTGTTGCTTTTGGGTTATGGTGGAACCAACAAT
AACCCAACCAAACAAAGACACAAGCACAACTTAACAACCATCTCAGCATT
CACCACCACGAAGACGGAGGACAAGATGGAGGGTGGACTCCTTTTGGATG
TTATAATCAGCAAGGGTACGGCCGTCCTCTAGCTGCTTCCCAGCGAAGAT
GAGCCTCTGCTGATCTGGTGGAATCCCTTCCTTGTCCTGAATCTTTGCCT
TGACATTATCAATTGTATCAGAACTCTCCACCTCCAGAGTAATAGTCTTC
CCAGTCAAAGTCTTGACAAAAATTTGCATGCCTCCACGGAGACGGAGGAC
AAGGTGAAGAGTTGACTCTTTCTGGATGTTATAGTCAGCCAACGTGCGGC
CGTCTTCCAATTGTTTTCCGGCAAAGATCAATCTTTGTTGATCTGGTGGG
ATCCCTTCCTTGTCTTGGATCTTGGCCTTGACATTATCAATCGTATCTGA
ACTCTC