CN184455

Overview
NameCN184455
Unique NameCN184455
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length393
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7281 contig Csv1_Contig7281:929..1319. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT11_ARATH (Actin-11 OS=Arabidopsis thaliana GN=ACT11 PE=1 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 5.190e-50
Identity = 77/78 (98.72%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 5.190e-50
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT7_SOLTU (Actin-75 OS=Solanum tuberosum GN=AC75 PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.765e-50
Identity = 78/78 (100.00%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 60.077 bits (144), Expect = 6.765e-50
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K E+DESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEFDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT3_SOLTU (Actin-58 OS=Solanum tuberosum GN=AC58 PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 8.817e-50
Identity = 76/78 (97.44%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMFPGIADRMSKE+TALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKELTALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 8.817e-50
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT1_PEA (Actin-1 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 8.818e-50
Identity = 77/78 (98.72%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  270 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 347

HSP 2 Score: 60.8474 bits (146), Expect = 8.818e-50
Identity = 27/29 (93.10%), Postives = 27/29 (93.10%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDE GPSIVHRKCF
Sbjct:  348 ASLSTFQQMWISKAEYDERGPSIVHRKCF 376
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT_COLSC (Actin OS=Coleochaete scutata PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.149e-49
Identity = 76/78 (97.44%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME+AGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MESAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 1.149e-49
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKSEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT1_TOBAC (Actin OS=Nicotiana tabacum PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.149e-49
Identity = 77/78 (98.72%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYG IVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGKIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 1.149e-49
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT_PINCO (Actin (Fragment) OS=Pinus contorta PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 1.164e-49
Identity = 78/78 (100.00%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:   55 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 132

HSP 2 Score: 59.3066 bits (142), Expect = 1.164e-49
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EY+ESGPSIVHRKCF
Sbjct:  133 ASLSTFQQMWIAKTEYEESGPSIVHRKCF 161
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT2_PEA (Actin-2 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.953e-49
Identity = 76/78 (97.44%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMFPGIADRMSKEITALAPSSMKIKVVAPPER+YSVWIGGSIL
Sbjct:  270 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERRYSVWIGGSIL 347

HSP 2 Score: 60.8474 bits (146), Expect = 1.953e-49
Identity = 27/29 (93.10%), Postives = 27/29 (93.10%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDE GPSIVHRKCF
Sbjct:  348 ASLSTFQQMWISKAEYDERGPSIVHRKCF 376
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT1_ARATH (Actin-1/3 OS=Arabidopsis thaliana GN=ACT1 PE=1 SV=2)

HSP 1 Score: 154.836 bits (390), Expect = 2.545e-49
Identity = 76/78 (97.44%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME AGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MENAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 2.545e-49
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT2_ORYSJ (Actin-2 OS=Oryza sativa subsp. japonica GN=ACT2 PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 5.635e-49
Identity = 77/78 (98.72%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEA GIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAPGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 58.9214 bits (141), Expect = 5.635e-49
Identity = 25/29 (86.21%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWIS+ EY+ESGP+IVHRKCF
Sbjct:  349 ASLSTFQQMWISRAEYEESGPAIVHRKCF 377
The following BLAST results are available for this feature:
BLAST of CN184455 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 417
Match NameE-valueIdentityDescription
ACT11_ARATH5.190e-5098.72Actin-11 OS=Arabidopsis thaliana GN=ACT11 PE=1 SV=... [more]
ACT7_SOLTU6.765e-50100.00Actin-75 OS=Solanum tuberosum GN=AC75 PE=3 SV=1[more]
ACT3_SOLTU8.817e-5097.44Actin-58 OS=Solanum tuberosum GN=AC58 PE=3 SV=1[more]
ACT1_PEA8.818e-5098.72Actin-1 OS=Pisum sativum PE=2 SV=1[more]
ACT_COLSC1.149e-4997.44Actin OS=Coleochaete scutata PE=2 SV=1[more]
ACT1_TOBAC1.149e-4998.72Actin OS=Nicotiana tabacum PE=3 SV=1[more]
ACT_PINCO1.164e-49100.00Actin (Fragment) OS=Pinus contorta PE=3 SV=1[more]
ACT2_PEA1.953e-4997.44Actin-2 OS=Pisum sativum PE=2 SV=1[more]
ACT1_ARATH2.545e-4997.44Actin-1/3 OS=Arabidopsis thaliana GN=ACT1 PE=1 SV=... [more]
ACT2_ORYSJ5.635e-4998.72Actin-2 OS=Oryza sativa subsp. japonica GN=ACT2 PE... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0005I15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005I15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184455 ID=CN184455; Name=CN184455; organism=Citrus sinensis; type=EST; length=393bp
GAGGATGGAAGCTGCTGGTATTCATGAGACTACCTACAACTCCATCATGA
AGTGTGATGTGGATATTAGAAAGGATCTGTATGGTAACATTGTTCTCAGT
GGGGGTTCAACTATGTTCCCCGGTATTGCCGACCGTATGAGCAAGGAAAT
CACAGCACTTGCTCCAAGCAGCATGAAGATCAAGGTCGTGGCTCCACCAG
AGAGAAAATACAGTGTTTGGATTGGAGGATCAATCCTTTGCATCCCTCAG
CACCTTCCAGCAGATGTGGATCTCAAAGGGCGAGTATGATGAGTCTGGTC
CATCCATTGTCCACAGGAAATGCTTCTAAGTTCTTTAAGTGCTTGATGGG
TGAGTTCTTTTTTTCCCATTTAGTTGGTTTTTTGTGTCTCGTT
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