CN184455

Overview
NameCN184455
Unique NameCN184455
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length393
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT2_ORYSI (Actin-2 OS=Oryza sativa subsp. indica GN=ACT2 PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 5.635e-49
Identity = 77/78 (98.72%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEA GIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAPGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 58.9214 bits (141), Expect = 5.635e-49
Identity = 25/29 (86.21%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWIS+ EY+ESGP+IVHRKCF
Sbjct:  349 ASLSTFQQMWISRAEYEESGPAIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT3_PEA (Actin-3 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 5.635e-49
Identity = 75/78 (96.15%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME+ GIHETT+NSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MESPGIHETTFNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 5.635e-49
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT6_SOLTU (Actin-71 OS=Solanum tuberosum GN=AC71 PE=3 SV=2)

HSP 1 Score: 153.295 bits (386), Expect = 7.345e-49
Identity = 76/78 (97.44%), Postives = 76/78 (97.44%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGN VLSGGSTMFPGIADRMSKEI ALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGNHVLSGGSTMFPGIADRMSKEIQALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 61.6178 bits (148), Expect = 7.345e-49
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  349 ASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT1_DAUCA (Actin-1 OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 1.248e-48
Identity = 76/79 (96.20%), Postives = 78/79 (98.73%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYS-VWIGGSIL 238
            ME+AGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYS +WIGGSIL
Sbjct:  270 MESAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSDLWIGGSIL 348

HSP 2 Score: 62.7734 bits (151), Expect = 1.248e-48
Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKC 323
            ASLSTFQQMWISKGEYDESGPSIVHRKC
Sbjct:  349 ASLSTFQQMWISKGEYDESGPSIVHRKC 376
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT8_ARATH (Actin-8 OS=Arabidopsis thaliana GN=ACT8 PE=1 SV=2)

HSP 1 Score: 154.066 bits (388), Expect = 1.248e-48
Identity = 76/78 (97.44%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMF GIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFSGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 60.077 bits (144), Expect = 1.248e-48
Identity = 26/29 (89.66%), Postives = 27/29 (93.10%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDE+GP IVHRKCF
Sbjct:  349 ASLSTFQQMWISKAEYDEAGPGIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT2_ARATH (Actin-2 OS=Arabidopsis thaliana GN=ACT2 PE=1 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.248e-48
Identity = 76/78 (97.44%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGG+TMF GIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL
Sbjct:  271 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFSGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 348

HSP 2 Score: 60.077 bits (144), Expect = 1.248e-48
Identity = 26/29 (89.66%), Postives = 27/29 (93.10%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDE+GP IVHRKCF
Sbjct:  349 ASLSTFQQMWISKAEYDEAGPGIVHRKCF 377
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT1_DICDI (Major actin OS=Dictyostelium discoideum GN=act1 PE=1 SV=2)

HSP 1 Score: 151.754 bits (382), Expect = 1.626e-48
Identity = 70/78 (89.74%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME+AGIHETTYNSIMKCDVDIRKDLYGN+VLSGG+TMFPGIADRM+KE+TALAPS+MKIK++APPERKYSVWIGGSIL
Sbjct:  270 MESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSIL 347

HSP 2 Score: 62.003 bits (149), Expect = 1.626e-48
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDESGPSIVHRKCF
Sbjct:  348 ASLSTFQQMWISKEEYDESGPSIVHRKCF 376
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT10_DICDI (Actin-10 OS=Dictyostelium discoideum GN=act10 PE=1 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 1.626e-48
Identity = 70/78 (89.74%), Postives = 78/78 (100.00%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME+AGIHETTYNSIMKCDVDIRKDLYGN+VLSGG+TMFPGIADRM+KE+TALAPS+MKIK++APPERKYSVWIGGSIL
Sbjct:  270 MESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSIL 347

HSP 2 Score: 62.003 bits (149), Expect = 1.626e-48
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDESGPSIVHRKCF
Sbjct:  348 ASLSTFQQMWISKEEYDESGPSIVHRKCF 376
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACTA_PHYPO (Actin, plasmodial isoform OS=Physarum polycephalum GN=ARDA PE=1 SV=2)

HSP 1 Score: 151.369 bits (381), Expect = 2.120e-48
Identity = 70/78 (89.74%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME+AGIHETTYNSIMKCDVDIRKDLYGN+VLSGG+TMFPGIADRM KE+TALAPS+MKIK++APPERKYSVWIGGSIL
Sbjct:  270 MESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMQKELTALAPSTMKIKIIAPPERKYSVWIGGSIL 347

HSP 2 Score: 62.003 bits (149), Expect = 2.120e-48
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDESGPSIVHRKCF
Sbjct:  348 ASLSTFQQMWISKEEYDESGPSIVHRKCF 376
[Close]
BLAST of CN184455 vs. ExPASy Swiss-Prot
Match: ACT1_ACACA (Actin-1 OS=Acanthamoeba castellanii PE=3 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 2.120e-48
Identity = 70/78 (89.74%), Postives = 77/78 (98.72%), Query Frame = 2 
Query:    5 MEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSIL 238
            ME+AGIHETTYNSIMKCDVDIRKDLYGN+VLSGG+TMFPGIADRM KE+TALAPS+MKIK++APPERKYSVWIGGSIL
Sbjct:  269 MESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMQKELTALAPSTMKIKIIAPPERKYSVWIGGSIL 346

HSP 2 Score: 62.003 bits (149), Expect = 2.120e-48
Identity = 28/29 (96.55%), Postives = 28/29 (96.55%), Query Frame = 3 
Query:  240 ASLSTFQQMWISKGEYDESGPSIVHRKCF 326
            ASLSTFQQMWISK EYDESGPSIVHRKCF
Sbjct:  347 ASLSTFQQMWISKEEYDESGPSIVHRKCF 375
The following BLAST results are available for this feature:
BLAST of CN184455 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 417
Match NameE-valueIdentityDescription
ACT2_ORYSI5.635e-4998.72Actin-2 OS=Oryza sativa subsp. indica GN=ACT2 PE=3... [more]
ACT3_PEA5.635e-4996.15Actin-3 OS=Pisum sativum PE=2 SV=1[more]
ACT6_SOLTU7.345e-4997.44Actin-71 OS=Solanum tuberosum GN=AC71 PE=3 SV=2[more]
ACT1_DAUCA1.248e-4896.20Actin-1 OS=Daucus carota PE=2 SV=1[more]
ACT8_ARATH1.248e-4897.44Actin-8 OS=Arabidopsis thaliana GN=ACT8 PE=1 SV=2[more]
ACT2_ARATH1.248e-4897.44Actin-2 OS=Arabidopsis thaliana GN=ACT2 PE=1 SV=1[more]
ACT1_DICDI1.626e-4889.74Major actin OS=Dictyostelium discoideum GN=act1 PE... [more]
ACT10_DICDI1.626e-4889.74Actin-10 OS=Dictyostelium discoideum GN=act10 PE=1... [more]
ACTA_PHYPO2.120e-4889.74Actin, plasmodial isoform OS=Physarum polycephalum... [more]
ACT1_ACACA2.120e-4889.74Actin-1 OS=Acanthamoeba castellanii PE=3 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0005I15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005I15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184455 ID=CN184455; Name=CN184455; organism=Citrus sinensis; type=EST; length=393bp
GAGGATGGAAGCTGCTGGTATTCATGAGACTACCTACAACTCCATCATGA
AGTGTGATGTGGATATTAGAAAGGATCTGTATGGTAACATTGTTCTCAGT
GGGGGTTCAACTATGTTCCCCGGTATTGCCGACCGTATGAGCAAGGAAAT
CACAGCACTTGCTCCAAGCAGCATGAAGATCAAGGTCGTGGCTCCACCAG
AGAGAAAATACAGTGTTTGGATTGGAGGATCAATCCTTTGCATCCCTCAG
CACCTTCCAGCAGATGTGGATCTCAAAGGGCGAGTATGATGAGTCTGGTC
CATCCATTGTCCACAGGAAATGCTTCTAAGTTCTTTAAGTGCTTGATGGG
TGAGTTCTTTTTTTCCCATTTAGTTGGTTTTTTGTGTCTCGTT
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