CN189059 | Citrus Genome Database

Overview


Name	CN189059
Unique Name	CN189059
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	789

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Rind cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig7217	contig	Csv1_Contig7217:1266..2052.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_STAAR (Aconitate hydratase OS=Staphylococcus aureus (strain MRSA252) GN=acnA PE=3 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 8.245e-44
Identity = 82/150 (54.67%), Postives = 101/150 (67.33%), Query Frame = -2

Query:  207 EKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGK----SFTCTVRFDTEVELAYFDHGGILPFVIRNLIKQ 644
            E + +FDAAMKYK  G G ++LAG +YG GSSRDWAAKG  LLGVK VIA+S+ERIHRSNLV MG++PL FK GE ADSLGL G E  S+++      ++P   V VT          F   VRFD+ VE+ Y+ HGGIL  V+RN + Q
Sbjct:  755 EVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNID---ENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ 901

HSP 2 Score: 35.8094 bits (81), Expect = 8.245e-44
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            R+FNS GSRRGN EVM RG F
Sbjct:  710 REFNSYGSRRGNHEVMVRGTF 730

HSP 3 Score: 29.6462 bits (65), Expect = 8.245e-44
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = -1

Query:  649 LEGTFANIRLVNKLLNGEVGPKTVHVPT 732
            + GTFANIR+ N+L  G  G  T + PT
Sbjct:  726 VRGTFANIRIKNQLAPGTEGGFTTYWPT 753

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_STAAN (Aconitate hydratase OS=Staphylococcus aureus (strain N315) GN=acnA PE=1 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 8.245e-44
Identity = 82/150 (54.67%), Postives = 101/150 (67.33%), Query Frame = -2

Query:  207 EKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGK----SFTCTVRFDTEVELAYFDHGGILPFVIRNLIKQ 644
            E + +FDAAMKYK  G G ++LAG +YG GSSRDWAAKG  LLGVK VIA+S+ERIHRSNLV MG++PL FK GE ADSLGL G E  S+++      ++P   V VT          F   VRFD+ VE+ Y+ HGGIL  V+RN + Q
Sbjct:  755 EVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNID---ENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ 901

HSP 2 Score: 35.8094 bits (81), Expect = 8.245e-44
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            R+FNS GSRRGN EVM RG F
Sbjct:  710 REFNSYGSRRGNHEVMVRGTF 730

HSP 3 Score: 29.6462 bits (65), Expect = 8.245e-44
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = -1

Query:  649 LEGTFANIRLVNKLLNGEVGPKTVHVPT 732
            + GTFANIR+ N+L  G  G  T + PT
Sbjct:  726 VRGTFANIRIKNQLAPGTEGGFTTYWPT 753

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_STAAM (Aconitate hydratase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=acnA PE=1 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 8.245e-44
Identity = 82/150 (54.67%), Postives = 101/150 (67.33%), Query Frame = -2

Query:  207 EKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGK----SFTCTVRFDTEVELAYFDHGGILPFVIRNLIKQ 644
            E + +FDAAMKYK  G G ++LAG +YG GSSRDWAAKG  LLGVK VIA+S+ERIHRSNLV MG++PL FK GE ADSLGL G E  S+++      ++P   V VT          F   VRFD+ VE+ Y+ HGGIL  V+RN + Q
Sbjct:  755 EVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNID---ENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ 901

HSP 2 Score: 35.8094 bits (81), Expect = 8.245e-44
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            R+FNS GSRRGN EVM RG F
Sbjct:  710 REFNSYGSRRGNHEVMVRGTF 730

HSP 3 Score: 29.6462 bits (65), Expect = 8.245e-44
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = -1

Query:  649 LEGTFANIRLVNKLLNGEVGPKTVHVPT 732
            + GTFANIR+ N+L  G  G  T + PT
Sbjct:  726 VRGTFANIRIKNQLAPGTEGGFTTYWPT 753

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_STAAC (Aconitate hydratase OS=Staphylococcus aureus (strain COL) GN=acnA PE=3 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 8.245e-44
Identity = 82/150 (54.67%), Postives = 101/150 (67.33%), Query Frame = -2

Query:  207 EKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGK----SFTCTVRFDTEVELAYFDHGGILPFVIRNLIKQ 644
            E + +FDAAMKYK  G G ++LAG +YG GSSRDWAAKG  LLGVK VIA+S+ERIHRSNLV MG++PL FK GE ADSLGL G E  S+++      ++P   V VT          F   VRFD+ VE+ Y+ HGGIL  V+RN + Q
Sbjct:  755 EVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNID---ENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ 901

HSP 2 Score: 35.8094 bits (81), Expect = 8.245e-44
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            R+FNS GSRRGN EVM RG F
Sbjct:  710 REFNSYGSRRGNHEVMVRGTF 730

HSP 3 Score: 29.6462 bits (65), Expect = 8.245e-44
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = -1

Query:  649 LEGTFANIRLVNKLLNGEVGPKTVHVPT 732
            + GTFANIR+ N+L  G  G  T + PT
Sbjct:  726 VRGTFANIRIKNQLAPGTEGGFTTYWPT 753

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON1_ECOLI (Aconitate hydratase 1 OS=Escherichia coli (strain K12) GN=acnA PE=1 SV=3)

HSP 1 Score: 146.747 bits (369), Expect = 3.067e-43
Identity = 76/147 (51.70%), Postives = 98/147 (66.67%), Query Frame = -2

Query:  210 LSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVT----TDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNLIK 638
            +S++DAAM+YK       ++AG EYGSGSSRDWAAKGP LLG++ VIA+SFERIHRSNL+GMGI+PL F  G    +LGLTG E+  I     +  ++PG  V VT      S +   C  R DT  EL Y+ + GIL +VIRN++K
Sbjct:  748 VSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDI---GDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK 891

HSP 2 Score: 39.6614 bits (91), Expect = 3.067e-43
Identity = 18/23 (78.26%), Postives = 19/23 (82.61%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            ER+DFNS GSRRGN EVM RG F
Sbjct:  699 ERKDFNSYGSRRGNHEVMMRGTF 721

HSP 3 Score: 30.8018 bits (68), Expect = 3.067e-43
Identity = 13/27 (48.15%), Postives = 19/27 (70.37%), Query Frame = -1

Query:  652 LEGTFANIRLVNKLLNGEVGPKTVHVP 732
            + GTFANIR+ N+++ G  G  T H+P
Sbjct:  717 MRGTFANIRIRNEMVPGVEGGMTRHLP 743

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_DEIRA (Aconitate hydratase OS=Deinococcus radiodurans GN=acn PE=1 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 4.497e-43
Identity = 86/154 (55.84%), Postives = 107/154 (69.48%), Query Frame = -2

Query:  207 FQQGEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVT--VTTDSGKSFTCTV--RFDTEVELAYFDHGGILPFVIRNLIKQ 656
            F  GE  S+FDA+  YK AG   ++LAG +YG GSSRDWAAKG  LLGVKAVIA+SFERIHRSNLVGMG++PL +K GE ADSLG+ G E F   LP    +++P QDVT  VT   G +   TV  R DT VE+ Y+ +GGIL  V+R ++ +
Sbjct:  749 FLNGEVTSIFDASTAYKEAGVPLVVLAGKDYGMGSSRDWAAKGTFLLGVKAVIAESFERIHRSNLVGMGVLPLQYKNGETADSLGINGDETFEFVLP---GDLKPRQDVTVKVTGKDGNTRDITVMCRIDTPVEIDYYKNGGILQTVLRGILSK 899

HSP 2 Score: 36.5798 bits (83), Expect = 4.497e-43
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            +DFNS GSRRGND +M RG F
Sbjct:  708 KDFNSYGSRRGNDRIMTRGTF 728

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_LEGPH (Aconitate hydratase OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=acn PE=3 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 3.259e-42
Identity = 76/148 (51.35%), Postives = 99/148 (66.89%), Query Frame = -2

Query:  216 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSG----KSFTCTVRFDTEVELAYFDHGGILPFVIRNL 647
            GE +S++DAAM+Y+      +I+AG EYG+GSSRDWAAKG  LLGVKAVI +SFERIHRSNL+GMGI+PL FK G    +L L G ER SI++  K++   PG  V VT +      +      R DT  EL Y+ +GGIL +V+R +
Sbjct:  745 GETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESFERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLT---PGAMVPVTIERQDGDIEKIETLCRIDTADELEYYKNGGILQYVLRKI 889

HSP 2 Score: 36.5798 bits (83), Expect = 3.259e-42
Identity = 16/23 (69.57%), Postives = 18/23 (78.26%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            + +DFNS GSRRGN EVM RG F
Sbjct:  699 DEKDFNSYGSRRGNHEVMMRGTF 721

HSP 3 Score: 34.2686 bits (77), Expect = 3.259e-42
Identity = 15/29 (51.72%), Postives = 21/29 (72.41%), Query Frame = -1

Query:  646 LEGTFANIRLVNKLLNGEVGPKTVHVPTG 732
            + GTFANIR+ N++  G+ G  T +VPTG
Sbjct:  717 MRGTFANIRIRNEMTPGQEGGVTRYVPTG 745

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_STRMU (Aconitate hydratase OS=Streptococcus mutans GN=acn PE=3 SV=2)

HSP 1 Score: 149.828 bits (377), Expect = 4.238e-42
Identity = 75/144 (52.08%), Postives = 103/144 (71.53%), Query Frame = -2

Query:  222 QGEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSG-KSFTCTVRFDTEVELAYFDHGGILPFVIR 650
            +GE L +++AAM YK  G  TI++AG +YG GSSRDWAAKG  LLGVK V+A+SFERIHRSNLV MGI+PL F  G+ A+SL LTG+E ++++LP +  ++     V  T+  G K F   +RFD + ++ Y+ +GGILP V+R
Sbjct:  740 EGEILPIYEAAMNYKKNGVSTIVIAGKDYGMGSSRDWAAKGANLLGVKVVLAESFERIHRSNLVMMGILPLQFLDGQTAESLQLTGYETYTVELPEQ-PQVHDIVKVKATSKEGTKEFQVLLRFDADADIRYYQNGGILPMVVR 882

HSP 2 Score: 33.4982 bits (75), Expect = 4.238e-42
Identity = 15/20 (75.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  719 DFNS*GSRRGNDEVMARGNF 778
            +FNS GSRRGN EVM RG F
Sbjct:  700 EFNSYGSRRGNHEVMMRGTF 719

HSP 3 Score: 30.0314 bits (66), Expect = 4.238e-42
Identity = 12/23 (52.17%), Postives = 18/23 (78.26%), Query Frame = -1

Query:  664 LEGTFANIRLVNKLLNGEVGPKT 732
            + GTFANIR+ N+L +G++G  T
Sbjct:  715 MRGTFANIRIKNELADGKIGGYT 737

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: IREB2_PIG (Iron-responsive element-binding protein 2 OS=Sus scrofa GN=IREB2 PE=2 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 8.264e-42
Identity = 79/163 (48.47%), Postives = 111/163 (68.10%), Query Frame = -2

Query:  222 SVLLTSS*MGKWVQRRSMFQQGEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGKSFTCTVRFDTEVELAYFDHGGILPFVIR 710
            ++ L +  +GK   +   F  G+ L VF+AA  Y+  G   IILAG +YGSG+SRDWAAKGP LLGVKAV+A+S+E+IH+ +L+G+GI PL F  GE+ADSLGL+G E FS+  P ++S   PG  + + T +GK F+    F+ +VE+  + HGG+L FV R
Sbjct:  802 NIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLQFLPGENADSLGLSGRETFSLTFPEELS---PGVTLNIKTSTGKIFSVIASFENDVEIILYKHGGLLNFVAR 961

HSP 2 Score: 35.039 bits (79), Expect = 8.264e-42
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            R+FNS G+RRGND VM RG F
Sbjct:  780 REFNSYGARRGNDAVMTRGTF 800

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: IREB2_RAT (Iron-responsive element-binding protein 2 OS=Rattus norvegicus GN=Ireb2 PE=1 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 1.077e-41
Identity = 79/166 (47.59%), Postives = 111/166 (66.87%), Query Frame = -2

Query:  213 SVLLTSS*MGKWVQRRSMFQQGEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNLI 710
            ++ L +  +GK   +   F  G+ L VF+AA  Y+  G   IILAG +YGSG+SRDWAAKGP LLGVKAV+A+S+E+IH+ +L+G+GI PL F  GE+ADSLGL+G E FS+  P    E+ PG  + + T +GK F+    F+ +VE+  + HGG+L FV R  +
Sbjct:  801 NIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLGLSGREVFSLSFP---EELFPGITLNIKTSTGKEFSVIAAFENDVEITLYKHGGLLNFVARKFL 963

HSP 2 Score: 35.039 bits (79), Expect = 1.077e-41
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = -3

Query:  719 RDFNS*GSRRGNDEVMARGNF 781
            R+FNS G+RRGND VM RG F
Sbjct:  779 REFNSYGARRGNDAVMTRGTF 799

The following BLAST results are available for this feature:

BLAST of CN189059 vs. ExPASy Swiss-Prot

BLAST of CN189059 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53


Match Name	E-value	Identity	Description
ACON_STAAR	8.245e-44	54.67	Aconitate hydratase OS=Staphylococcus aureus (stra...	[more]
ACON_STAAN	8.245e-44	54.67	Aconitate hydratase OS=Staphylococcus aureus (stra...	[more]
ACON_STAAM	8.245e-44	54.67	Aconitate hydratase OS=Staphylococcus aureus (stra...	[more]
ACON_STAAC	8.245e-44	54.67	Aconitate hydratase OS=Staphylococcus aureus (stra...	[more]
ACON1_ECOLI	3.067e-43	51.70	Aconitate hydratase 1 OS=Escherichia coli (strain ...	[more]
ACON_DEIRA	4.497e-43	55.84	Aconitate hydratase OS=Deinococcus radiodurans GN=...	[more]
ACON_LEGPH	3.259e-42	51.35	Aconitate hydratase OS=Legionella pneumophila subs...	[more]
ACON_STRMU	4.238e-42	52.08	Aconitate hydratase OS=Streptococcus mutans GN=acn...	[more]
IREB2_PIG	8.264e-42	48.47	Iron-responsive element-binding protein 2 OS=Sus s...	[more]
IREB2_RAT	1.077e-41	47.59	Iron-responsive element-binding protein 2 OS=Rattu...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS06_0001E02_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001E02, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN189059 ID=CN189059; Name=CN189059; organism=Citrus sinensis; type=EST; length=789bp
GAGGAAAGAATTGTATTACTGAAAATAATTCTTCGTTCACAGAACAAAGA
TATCGACCTTTTGAATTGTTCCCAGAAAAAAAGACTCAAGTCCCATCAAA
GGGATCTTTCTTACATTATTTGAACTTTATTCAACTCTCACATTTTACAA
CCGTAAGTTTCTTTGGATGAAGCAAGTCCGCCTAGTAGAAATGATGCAGT
TATTTACTGCTTAATCAGGTTGCGAATGACAAACGGAAGGATGCCTCCAT
GATCAAAGTAAGCCAATTCTACCTCTGTGTCAAAACGGACTGTGCAGGTA
AAGGATTTTCCACTGTCAGTGGTGACAGTCACATCTTGGCCAGGTCTTAT
TTCACTGATCTTACTTGGGAGATCAATACTAAATCTCTCATGACCAGTCA
AACCCAATGAGTCTGCATCCTCACCAGCCTTGAAACAGAGAGGAATGATA
CCCATTCCAACCAAATTACTGCGATGAATTCTTTCAAAACTTTTGGCGAT
CACTGCTTTAACTCCCAACAACATTGGACCCTTGGCAGCCCAATCACGGG
AACTTCCACTTCCATATTCAGCTCCAGCCAAGATAATTGTACCATGACCA
GCACTCTTGTATTTCATTGCTGCATCAAAAACAGAGAGTTTCTCCCCCTG
TTGGAACATGGACCGTCTTTGGACCCACTTCCCCATTCAAGAGCTTGTTA
ACAAGACGGATATTTGCAAAAGTTCCCTCTAGCCATCACTTCATCATTGC
CACGACGACTACCCTAAGAATTGAAGTCCCTGCGTTCCA