CN189059 | Citrus Genome Database

Overview


Name	CN189059
Unique Name	CN189059
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	789

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Rind cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig7217	contig	Csv1_Contig7217:1266..2052.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_FRATH (Aconitate hydratase OS=Francisella tularensis subsp. holarctica (strain LVS) GN=acn PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 2.314e-38
Identity = 77/150 (51.33%), Postives = 102/150 (68.00%), Query Frame = -2

Query:  213 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTV-----TTDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNLI 647
            G +  VFDAAMKYK  G   +ILAG EYG+GSSRDWAAKG  LLGVKAVIA+S+ERIHRSNLVGMG++PL +  G++A +LGL G E F+I     ++ I+P Q V V      T    +F    R D +V++ Y  +GGIL  V+++++
Sbjct:  749 GSQQYVFDAAMKYKEKGIPLVILAGKEYGTGSSRDWAAKGTFLLGVKAVIAESYERIHRSNLVGMGVLPLEYVNGQNAKTLGLDGTEMFNI---KNLNNIKPRQIVIVEAVHPKTAHTTTFEALARLDADVDVDYLKNGGILQTVLKDIM 895

HSP 2 Score: 38.5058 bits (88), Expect = 2.314e-38
Identity = 17/23 (73.91%), Postives = 19/23 (82.61%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            E++DFNS GSRRGN EVM RG F
Sbjct:  703 EKKDFNSYGSRRGNHEVMMRGTF 725

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_RICBR (Aconitate hydratase OS=Rickettsia bellii (strain RML369-C) GN=acnA PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.221e-35
Identity = 73/144 (50.69%), Postives = 92/144 (63.89%), Query Frame = -2

Query:  216 SVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDV--TVTTDSGKSFT--CTVRFDTEVELAYFDHGGILPFVIRNL 635
            +++DAAM YK+ G   +I AG EYGSGSSRDWAAKGP LLGVKAVIA+SFERIHRSNLVGMG++PL F        L L G E  SID+      I+P   V   +   +G+  T    ++  T+ E+ Y  HG I+ FV+ NL
Sbjct:  739 TIYDAAMDYKANGVSAVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGVLPLIFTNNMTRFDLKLDGSE--SIDIIGLNEHIKPYNSVKCIIKKQNGEMQTIDLILQIFTDNEINYIKHGSIMHFVVENL 880

HSP 2 Score: 35.039 bits (79), Expect = 2.221e-35
Identity = 16/20 (80.00%), Postives = 16/20 (80.00%), Query Frame = -3

Query:  719 DFNS*GSRRGNDEVMARGNF 778
            DFNS GSRRGN EVM RG F
Sbjct:  692 DFNSYGSRRGNHEVMMRGTF 711

HSP 3 Score: 26.9498 bits (58), Expect = 2.221e-35
Identity = 11/25 (44.00%), Postives = 17/25 (68.00%), Query Frame = -1

Query:  658 LEGTFANIRLVNKLLNGEVGPKTVH 732
            + GTFANIR+ N++  G  G  T++
Sbjct:  707 MRGTFANIRIKNEMCKGVEGGFTIN 731

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_RICFE (Aconitate hydratase OS=Rickettsia felis GN=acnA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 3.750e-35
Identity = 71/144 (49.31%), Postives = 90/144 (62.50%), Query Frame = -2

Query:  216 SVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTV----TTDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNL 635
            +++DAAM YK+     +I AG EYGSGSSRDWAAKGP LLGVKAVIA+SFERIHRSNLVGMGI+PL F        L L G E  +ID+     +I+P   V       T   ++    ++  T+ E+ Y  HG I+ FV+ NL
Sbjct:  735 TIYDAAMDYKANDVPVVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGILPLTFTGNNTRLDLKLDGSE--TIDIIGLSEQIKPYNPVKCMIKKQTGETRTIDLILQIFTDNEINYIKHGSIMHFVVENL 876

HSP 2 Score: 35.4242 bits (80), Expect = 3.750e-35
Identity = 17/23 (73.91%), Postives = 17/23 (73.91%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            E  DFNS GSRRGN EVM RG F
Sbjct:  685 EPLDFNSYGSRRGNHEVMMRGTF 707

HSP 3 Score: 26.9498 bits (58), Expect = 3.750e-35
Identity = 11/25 (44.00%), Postives = 17/25 (68.00%), Query Frame = -1

Query:  658 LEGTFANIRLVNKLLNGEVGPKTVH 732
            + GTFANIR+ N++  G  G  T++
Sbjct:  703 MRGTFANIRIKNEMCKGVEGGFTIN 727

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_RICCN (Aconitate hydratase OS=Rickettsia conorii GN=acnA PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 6.332e-35
Identity = 72/146 (49.32%), Postives = 91/146 (62.33%), Query Frame = -2

Query:  216 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSI-DLPSKISEIRPGQDV-TVTTDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNL 647
            G + +++DAAM YK+     +I AG EYGSGSSRDWAAKGP LLGVKAVIA+SFERIHRSNLVGMGI+PL F        L L G E   I  L   IS   P + V    T + ++    ++  T+ E+ Y  HG I+ FV+ +L
Sbjct:  731 GTQQTIYDAAMDYKAHDVSVVIFAGKEYGSGSSRDWAAKGPGLLGVKAVIAESFERIHRSNLVGMGILPLTFTGNNTRLDLKLDGSETIDITGLSENISSYHPVKCVIKKQTGAIRTIDLILQIFTDNEINYIKHGSIMHFVVESL 876

HSP 2 Score: 35.4242 bits (80), Expect = 6.332e-35
Identity = 17/23 (73.91%), Postives = 17/23 (73.91%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            E  DFNS GSRRGN EVM RG F
Sbjct:  685 EPLDFNSYGSRRGNHEVMMRGTF 707

HSP 3 Score: 27.335 bits (59), Expect = 6.332e-35
Identity = 12/29 (41.38%), Postives = 18/29 (62.07%), Query Frame = -1

Query:  646 LEGTFANIRLVNKLLNGEVGPKTVHVPTG 732
            + GTFANIR+ N++  G  G  T++   G
Sbjct:  703 MRGTFANIRIKNEMCKGVEGGFTINQLNG 731

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_RICTY (Aconitate hydratase OS=Rickettsia typhi GN=acnA PE=3 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 1.389e-34
Identity = 70/148 (47.30%), Postives = 92/148 (62.16%), Query Frame = -2

Query:  216 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVT----TDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNL 647
            G + +++DAAM Y++     ++ AG EYGSGSSRDWAAKGP LLG+KA+IA+SFERIHRSNLVGMGI+PL F       SL L G E  +ID+      IRP   V       T+   +    ++  TE E+ Y  HG I+ FV+ +L
Sbjct:  731 GVQQTIYDAAMDYQAHDIPLVVFAGKEYGSGSSRDWAAKGPGLLGIKAIIAESFERIHRSNLVGMGILPLTFTGNNTRLSLKLDGSE--TIDIIGLSKNIRPFNLVKCVIKKQTNEISTIDLILQIFTENEINYIKHGSIMQFVVESL 876

HSP 2 Score: 33.4982 bits (75), Expect = 1.389e-34
Identity = 16/23 (69.57%), Postives = 17/23 (73.91%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            E  +FNS GSRRGN EVM RG F
Sbjct:  685 EPLNFNSYGSRRGNHEVMMRGTF 707

HSP 3 Score: 30.0314 bits (66), Expect = 1.389e-34
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = -1

Query:  646 LEGTFANIRLVNKLLNGEVGPKTVHVPTG 732
            + GTFANIR+ N++ NG  G  T++  +G
Sbjct:  703 MRGTFANIRIKNEMCNGVEGGFTINQLSG 731

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_RICPR (Aconitate hydratase OS=Rickettsia prowazekii GN=acnA PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.344e-34
Identity = 71/148 (47.97%), Postives = 90/148 (60.81%), Query Frame = -2

Query:  216 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVT----TDSGKSFTCTVRFDTEVELAYFDHGGILPFVIRNL 647
            G + +++D AM YK+     +I AG EYGSGSSRDWAAKGP LLG+KAVIA+SFERIHRSNLVGMGI+PL F       +L L G E   IDL      I+P   V       T+   +    ++  TE E+ Y  HG I+ FV+ +L
Sbjct:  731 GVQQTIYDTAMDYKAHDIPLVIFAGKEYGSGSSRDWAAKGPGLLGIKAVIAESFERIHRSNLVGMGILPLTFTGKNTRLNLKLDGSE--IIDLIGLSENIKPYNLVKCVIKKQTNEISTIDLILQIFTENEINYIKHGSIMQFVVESL 876

HSP 2 Score: 35.4242 bits (80), Expect = 2.344e-34
Identity = 17/23 (73.91%), Postives = 17/23 (73.91%), Query Frame = -3

Query:  719 ERRDFNS*GSRRGNDEVMARGNF 787
            E  DFNS GSRRGN EVM RG F
Sbjct:  685 EPLDFNSYGSRRGNHEVMMRGTF 707

HSP 3 Score: 30.0314 bits (66), Expect = 2.344e-34
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = -1

Query:  646 LEGTFANIRLVNKLLNGEVGPKTVHVPTG 732
            + GTFANIR+ N++ NG  G  T++  +G
Sbjct:  703 MRGTFANIRIKNEMCNGVEGGFTINQLSG 731

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_MYCAV (Aconitate hydratase OS=Mycobacterium avium GN=acn PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 5.130e-34
Identity = 73/150 (48.67%), Postives = 96/150 (64.00%), Query Frame = -2

Query:  210 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGKS----FTCTVRFDTEVELAYFDHGGILPFVIRNLIK 647
            G +  ++DAA  Y +     ++L G EYGSGSSRDWAAKG  LLGV+AVIA+SFERIHRSNL+GMG+IPL F  G+ A  LGL G E F I    ++++ +  + V V      S    F   VR DT  E  Y+ +GGIL +V+RN++K
Sbjct:  810 GPQAFIYDAAQNYAAQNIPLVVLGGKEYGSGSSRDWAAKGTRLLGVRAVIAESFERIHRSNLIGMGVIPLQFPDGKSAKDLGLDGTEVFDITGIEELNKGKTPKTVHVKASKNGSDAAEFDAVVRIDTPGEADYYRNGGILQYVLRNMLK 959

HSP 2 Score: 26.1794 bits (56), Expect = 5.130e-34
Identity = 11/15 (73.33%), Postives = 11/15 (73.33%), Query Frame = -3

Query:  719 GSRRGNDEVMARGNF 763
            G RRGN EVM RG F
Sbjct:  770 GCRRGNHEVMIRGTF 784

HSP 3 Score: 23.483 bits (49), Expect = 5.130e-34
Identity = 12/23 (52.17%), Postives = 14/23 (60.87%), Query Frame = -1

Query:  664 LEGTFANIRLVNKLLNGEVGPKT 732
            + GTFANIRL N L +   G  T
Sbjct:  780 IRGTFANIRLRNLLHDDVAGGYT 802

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_CORJK (Aconitate hydratase OS=Corynebacterium jeikeium (strain K411) GN=acn PE=2 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 4.281e-33
Identity = 79/159 (49.69%), Postives = 102/159 (64.15%), Query Frame = -2

Query:  213 RSMFQQGEKLS-VFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQ-----DVTVTTDSGK--SFTCTVRFDTEVELAYFDHGGILPFVIRNLI 665
            R   Q+G   S ++DAAM Y+      ++L G EYG+GSSRDWAAKG +LLGVKAVIA+SFERIHRSNL+GMG++PL F  GE   SLG+ G E F I+    I E+  G       VT T ++G+   F    R DT  E  Y+ +GGIL FV+RN++
Sbjct:  778 RDFTQEGGPQSFIYDAAMNYQKENTPLVVLGGKEYGTGSSRDWAAKGTLLLGVKAVIAESFERIHRSNLIGMGVVPLQFPEGESWKSLGIEGTETFDIE---GIEELNNGSTPKTVKVTATKENGEKIEFDAVTRIDTPGEADYYRNGGILQFVLRNMM 933

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_CANAL (Aconitate hydratase, mitochondrial OS=Candida albicans GN=ACO1 PE=1 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 2.209e-13
Identity = 51/144 (35.42%), Postives = 76/144 (52.78%), Query Frame = -2

Query:  228 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDV--TVTTDSGKSF--TCTVRFDTEVELAYFDHGGILPFV 647
            G+   V   A  Y+ AGH  +++    +G GSSR+ AA  P  LG  A+I KSF RIH +NL   G++PL FK   D D +         +DL   ++ + PG+DV   V    G+++    T  F++E +L +F HG  L F+
Sbjct:  636 GKYDGVPQTAAAYRDAGHKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETNLKKQGLLPLNFKNPADYDKINFDD----EVDLIG-LTTLAPGKDVILRVHPKEGEAWEAVLTHTFNSE-QLEWFKHGSALNFI 773

[Close]

BLAST of CN189059 vs. ExPASy Swiss-Prot
Match: ACON_DICDI (Probable aconitate hydratase, mitochondrial OS=Dictyostelium discoideum GN=aco2 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.870e-12
Identity = 45/114 (39.47%), Postives = 61/114 (53.51%), Query Frame = -2

Query:  306 GEKLSVFDAAMKYKSAGHGTIILAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERFSIDLPSKISEIRPGQDVTVTTDSGK 647
            GE   V   A  YK  G   I++    YG GSSR+ AA  P  LG KA++ KSF RIH +NL   GI+PL F    D D   ++G +R SI     + ++ PG+ +T+   S K
Sbjct:  625 GEIGPVPTVARDYKKRGVNWIVVGDENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGILPLTFANPSDYDK--ISGDDRISI---IGLKDLAPGKQLTLIVKSAK 733

The following BLAST results are available for this feature:

BLAST of CN189059 vs. ExPASy Swiss-Prot

BLAST of CN189059 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53


Match Name	E-value	Identity	Description
ACON_FRATH	2.314e-38	51.33	Aconitate hydratase OS=Francisella tularensis subs...	[more]
ACON_RICBR	2.221e-35	50.69	Aconitate hydratase OS=Rickettsia bellii (strain R...	[more]
ACON_RICFE	3.750e-35	49.31	Aconitate hydratase OS=Rickettsia felis GN=acnA PE...	[more]
ACON_RICCN	6.332e-35	49.32	Aconitate hydratase OS=Rickettsia conorii GN=acnA ...	[more]
ACON_RICTY	1.389e-34	47.30	Aconitate hydratase OS=Rickettsia typhi GN=acnA PE...	[more]
ACON_RICPR	2.344e-34	47.97	Aconitate hydratase OS=Rickettsia prowazekii GN=ac...	[more]
ACON_MYCAV	5.130e-34	48.67	Aconitate hydratase OS=Mycobacterium avium GN=acn ...	[more]
ACON_CORJK	4.281e-33	49.69	Aconitate hydratase OS=Corynebacterium jeikeium (s...	[more]
ACON_CANAL	2.209e-13	35.42	Aconitate hydratase, mitochondrial OS=Candida albi...	[more]
ACON_DICDI	1.870e-12	39.47	Probable aconitate hydratase, mitochondrial OS=Dic...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS06_0001E02_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001E02, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN189059 ID=CN189059; Name=CN189059; organism=Citrus sinensis; type=EST; length=789bp
GAGGAAAGAATTGTATTACTGAAAATAATTCTTCGTTCACAGAACAAAGA
TATCGACCTTTTGAATTGTTCCCAGAAAAAAAGACTCAAGTCCCATCAAA
GGGATCTTTCTTACATTATTTGAACTTTATTCAACTCTCACATTTTACAA
CCGTAAGTTTCTTTGGATGAAGCAAGTCCGCCTAGTAGAAATGATGCAGT
TATTTACTGCTTAATCAGGTTGCGAATGACAAACGGAAGGATGCCTCCAT
GATCAAAGTAAGCCAATTCTACCTCTGTGTCAAAACGGACTGTGCAGGTA
AAGGATTTTCCACTGTCAGTGGTGACAGTCACATCTTGGCCAGGTCTTAT
TTCACTGATCTTACTTGGGAGATCAATACTAAATCTCTCATGACCAGTCA
AACCCAATGAGTCTGCATCCTCACCAGCCTTGAAACAGAGAGGAATGATA
CCCATTCCAACCAAATTACTGCGATGAATTCTTTCAAAACTTTTGGCGAT
CACTGCTTTAACTCCCAACAACATTGGACCCTTGGCAGCCCAATCACGGG
AACTTCCACTTCCATATTCAGCTCCAGCCAAGATAATTGTACCATGACCA
GCACTCTTGTATTTCATTGCTGCATCAAAAACAGAGAGTTTCTCCCCCTG
TTGGAACATGGACCGTCTTTGGACCCACTTCCCCATTCAAGAGCTTGTTA
ACAAGACGGATATTTGCAAAAGTTCCCTCTAGCCATCACTTCATCATTGC
CACGACGACTACCCTAAGAATTGAAGTCCCTGCGTTCCA