CN188997

Overview
NameCN188997
Unique NameCN188997
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length686
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN188997_ssr207 microsatellite CN188997_ssr207:207..218. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9569 contig Csv1_Contig9569:980..1665. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.279e-20
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGG 76

HSP 2 Score: 97.4413 bits (241), Expect = 7.304e-20
Identity = 44/75 (58.67%), Postives = 58/75 (77.33%), Query Frame = -2 
Query:  458 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 682
            VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    5 VKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 1.627e-19
Identity = 45/73 (61.64%), Postives = 58/73 (79.45%), Query Frame = -2 
Query:  464 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 682
            VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    5 VKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 95.9005 bits (237), Expect = 2.125e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -2 
Query:  239 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.378e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.6633 bits (226), Expect = 4.008e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    5 VKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.378e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.6633 bits (226), Expect = 4.008e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    5 VKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.378e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.6633 bits (226), Expect = 4.008e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    5 VKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.378e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.6633 bits (226), Expect = 4.008e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    5 VKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.378e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 91.6633 bits (226), Expect = 4.008e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    5 VKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.799e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 3.069e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    5 VRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.166e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 87.0409 bits (214), Expect = 9.872e-17
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = -2 
Query:  467 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 682
            VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    5 VKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of CN188997 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 2.598e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  239 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 466
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76

HSP 2 Score: 88.1965 bits (217), Expect = 4.431e-17
Identity = 39/73 (53.42%), Postives = 56/73 (76.71%), Query Frame = -2 
Query:  464 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 682
            VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    5 VKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77
The following BLAST results are available for this feature:
BLAST of CN188997 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
NEDD8_DROME3.279e-2059.21NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=... [more]
RUB2_ARATH1.627e-1961.64NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
NEDD8_RAT1.378e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.378e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.378e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.378e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.378e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ORYSJ1.799e-1853.95NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
RUB3_ARATH1.166e-1755.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI2.598e-1753.95NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001C13_f Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001C13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188997 ID=CN188997; Name=CN188997; organism=Citrus sinensis; type=EST; length=686bp
TTTTTTTTTAATAAAGTACTGCACATTAAACATGTATTTACTCAAGAAAA
AGATTCTTAGATATTATAATGGGACGTTAGTGGGACCATATCAATATCTT
AGAAACAGAGTTCAACTACCACAACCAAAATAAACTTCAAATAGTTATTT
AAGCCATTTCACCAATAGCAAGCAAACATTAACCAAGACAAACCGAAGCA
AATAAGACACACACACACCCAGATGACCAAACTCAGAAACCTCCACGGAG
ACGGAGCACAAGATGCAGAGTGGACTCCTTCTGGATGTTGTAATCAGCAA
GAGTCCTTCCATCCTCAAGTTGTTTTCCAGCAAAGATCAACCTCTGCTGG
TCTGGGGGAATCCCCTCCTTATCCTGGATCTTAGCCTTCACATTGTCAAT
TGTGTCCGAACTCTCAACCTCCAATGTGATGGTTTTCCCAGTGAGTGTCT
TCACAAAAATCTGCATACCACCACGGAGGCGAAGAACCAAGTGAAGAGTG
GATTCTTTCTGGATGTTATAGTCTGCAAGAGTCCTTCCATCTTCAAGCTG
CTTTCCAGCAAAAATGAGCCTCTGCTGATCTGGTGGGATACCCTCCTTGT
CTTGAATTTTTGCTTTCACGTTGTCGATGGTGTCAGAACTTTCAACCTCA
AGAGTAATTGTTTTTCCGGTGAGGGTTTTCACAAAG
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