CN182055

Overview
NameCN182055
Unique NameCN182055
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length686
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: CDPKG_ARATH (Calcium-dependent protein kinase 16 OS=Arabidopsis thaliana GN=CPK16 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.631e-11
Identity = 43/154 (27.92%), Postives = 89/154 (57.79%), Query Frame = 3
Query:   60 MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNP---TEAELQDMINEVDADGNGTIDFPEFLN--LMARKMKDTDSE---EELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVM 497
            +A  L ++++++ ++ F   D D +G I+ +E+   +     +P    +A + +++  +D++ +G +DF EF+   L   ++++ DSE   +  + AF  FD D +GFI+A ELR    + G K     ++ ++ EAD+D DG+I+ +EF +++
Sbjct:  404 LATTLDEEELADLRDQFDAIDVDKNGVISLEEMRQALAK--DHPWKLKDARVAEILQAIDSNTDGFVDFGEFVAAALHVNQLEEHDSEKWQQRSRAAFEKFDIDGDGFITAEELR---MHTGLK---GSIEPLLEEADIDNDGKISLQEFRRLL 549          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: EFCB2_HUMAN (EF-hand calcium-binding domain-containing protein 2 OS=Homo sapiens GN=EFCAB2 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.782e-11
Identity = 40/105 (38.10%), Postives = 60/105 (57.14%), Query Frame = 3
Query:   93 EFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEV-DADGNGTIDFPEFLNLMARKMKDTD----SEEELKEAFRVFDKDQNGFISAAELRHVMT 392
            + KEAF +FD + +  +  +E+GT++RSLG  PTE EL D+I EV + +  G I F +FL +M   + +       E+ L  AF V D  + GF++  EL   MT
Sbjct:  154 KIKEAFEVFDHESNNTVDVREIGTIIRSLGCCPTEGELHDLIAEVEEEEPTGYIRFEKFLPVMTEILLERKYRPIPEDVLLRAFEVLDSAKRGFLTKDELIKYMT 258          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: SCMC2_XENLA (Calcium-binding mitochondrial carrier protein SCaMC-2 OS=Xenopus laevis GN=slc25a25 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.633e-11
Identity = 35/126 (27.78%), Postives = 70/126 (55.56%), Query Frame = 3
Query:  108 FSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEF 485
            F   D + DG I   +L   ++ LG + TE EL+ ++   D D +G +DF EF++ +       D E++L+  F+  DK  +G I A E+   + +LG  +++++ +++++  D +G   I++ E+
Sbjct:   65 FQELDVNKDGAICINDLAVGLKRLGVHRTELELRKIVKAGDKDQDGQLDFDEFVHYL------RDHEKKLRLVFKSLDKKNDGRIDAQEIMQSLRDLGVNISEQQAEKILKSMDKNGTMTIDWNEW 184          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: EFH52_TRYCR (EF-hand protein 5 variant 2 OS=Trypanosoma cruzi PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.633e-11
Identity = 38/126 (30.16%), Postives = 74/126 (58.73%), Query Frame = 3
Query:  141 ITTKELGTVMRSLGQNPTEAELQD---MINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVD-GDGQINYEEFVKVMMAK 506
            I +K++  +M+++G + TE E  D   +I + +      + F +FL LM R++ DT ++E L+ AF  +DK + G+++  +   +   L E+ T EE++E++  A+VD  D +I+Y  FV  + ++
Sbjct:   80 IPSKDVYALMQNVGMHLTEEEFHDALRVIGQSEPQNADELSFSDFLLLMTREVDDTMADE-LRSAFFHYDKHKTGYVTRKQFTELFATLAERSTPEELEELLAVAEVDETDDKIDYNRFVNELTSR 204          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: MLC1_DROPS (Myosin light chain alkali OS=Drosophila pseudoobscura pseudoobscura GN=Mlc1 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.745e-11
Identity = 43/144 (29.86%), Postives = 75/144 (52.08%), Query Frame = 3
Query:   84 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA--RKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREA--DVDGDGQINYEEFVKVMMA 503
            +I   +  F +    G+G I   +LG  +R+L  NPT A ++ M           I   EFL + +  +K K+    E+  E  +++DK++NG +  AEL+H +  LGE L DE+V+ +  +     D +G I Y +F++ +M+
Sbjct:    8 EIENVEFVFEVMGSAGEG-IDAVDLGDALRALNLNPTLALIEKM-GGTKKRNEKKIKMDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMMLAELQHALLALGESLDDEQVETLFADCMDPEDDEGLIPYSQFIQRLMS 149          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: ACTN2_HUMAN (Alpha-actinin-2 OS=Homo sapiens GN=ACTN2 PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.745e-11
Identity = 35/106 (33.02%), Postives = 64/106 (60.38%), Query Frame = 3
Query:   63 ADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELR 380
            A  +T +Q++EF+ +F+ FD+  +G +  ++    + S+G +  EAE   ++  VD +G GT+ F  F++ M R+  DTD+ E++  +FR+   D+  +I A ELR
Sbjct:  747 AKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDK-PYILAEELR 851          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: ACTN2_BOVIN (Alpha-actinin-2 OS=Bos taurus GN=ACTN2 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.745e-11
Identity = 35/106 (33.02%), Postives = 64/106 (60.38%), Query Frame = 3
Query:   63 ADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELR 380
            A  +T +Q++EF+ +F+ FD+  +G +  ++    + S+G +  EAE   ++  VD +G GT+ F  F++ M R+  DTD+ E++  +FR+   D+  +I A ELR
Sbjct:  747 AKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDK-PYILAEELR 851          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: SCMC2_XENTR (Calcium-binding mitochondrial carrier protein SCaMC-2 OS=Xenopus tropicalis GN=slc25a25 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.094e-11
Identity = 35/126 (27.78%), Postives = 70/126 (55.56%), Query Frame = 3
Query:  108 FSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEF 485
            F   D + DG I   +L   ++ LG + TE EL+ ++   D D +G +DF EF++ +       D E++L+  F+  DK  +G I A E+   + +LG  +++++ +++++  D +G   I++ E+
Sbjct:   64 FQELDVNKDGGICINDLAVGLKRLGVHRTELELRKIVKAGDKDQDGQLDFEEFVHYL------RDHEKKLRLVFKSLDKKNDGRIDAQEIMQSLRDLGVNISEQQAEKILKSMDKNGTMTIDWNEW 183          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: MLC1_DROVI (Myosin light chain alkali OS=Drosophila virilis GN=Mlc1 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.094e-11
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 3
Query:   84 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA--RKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREA--DVDGDGQINYEEFVKVMMA 503
            ++   +  F +    G+G I   +LG  +R+L  NPT A ++ M           I   EFL + +  +K K+    E+  E  +++DK++NG +  AEL+H +  LGE L DE+V+ +  +     D +G I Y +F++ +M+
Sbjct:    8 EVENVEFVFEVMGSPGEG-IDAFDLGDALRALNLNPTLALIEKM-GGTKKRNEKKIKLDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMLLAELQHALLALGESLDDEQVENLFADCMDPEDDEGFIPYSQFIQRLMS 149          
BLAST of CN182055 vs. ExPASy Swiss-Prot
Match: MLC1_DROSI (Myosin light chain alkali OS=Drosophila simulans GN=Mlc1 PE=4 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 8.094e-11
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 3
Query:   84 QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA--RKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREA--DVDGDGQINYEEFVKVMMA 503
            ++   +  F +    G+G I   +LG  +R+L  NPT A L + +          I   EFL + +  +K K+    E+  E  +++DK++NG +  AEL+H +  LGE L DE+V+ +  +     D +G I Y +F++ +M+
Sbjct:    8 EVENVEFVFEVMGSPGEG-IDAVDLGDALRALNLNPTLA-LIEKLGGTKKRNEKKIKLDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMMLAELQHALLALGESLDDEQVETLFADCMDPEDDEGFIPYSQFIQRLMS 149          
The following BLAST results are available for this feature:
BLAST of CN182055 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 482
Match NameE-valueIdentityDescription
CDPKG_ARATH1.631e-1127.92Calcium-dependent protein kinase 16 OS=Arabidopsis... [more]
EFCB2_HUMAN2.782e-1138.10EF-hand calcium-binding domain-containing protein ... [more]
SCMC2_XENLA3.633e-1127.78Calcium-binding mitochondrial carrier protein SCaM... [more]
EFH52_TRYCR3.633e-1130.16EF-hand protein 5 variant 2 OS=Trypanosoma cruzi P... [more]
MLC1_DROPS4.745e-1129.86Myosin light chain alkali OS=Drosophila pseudoobsc... [more]
ACTN2_HUMAN4.745e-1133.02Alpha-actinin-2 OS=Homo sapiens GN=ACTN2 PE=1 SV=1[more]
ACTN2_BOVIN4.745e-1133.02Alpha-actinin-2 OS=Bos taurus GN=ACTN2 PE=2 SV=1[more]
SCMC2_XENTR8.094e-1127.78Calcium-binding mitochondrial carrier protein SCaM... [more]
MLC1_DROVI8.094e-1129.17Myosin light chain alkali OS=Drosophila virilis GN... [more]
MLC1_DROSI8.094e-1129.17Myosin light chain alkali OS=Drosophila simulans G... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS04_0001O05_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001O05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN182055 ID=CN182055; Name=CN182055; organism=Citrus sinensis; type=EST; length=686bp
GAGGATTTGATTTTGCGGGGAAAAAAAAAACCTAGTTTTCGGTGTAAAAA
AAGAAGAAAATGGCGGATCAGCTCACCGACGATCAGATCTCCGAGTTTAA
AGAAGCCTTCAGCTTGTTCGACAAGGACGGCGACGGTTGCATTACCACCA
AGGAGCTTGGCACTGTTATGAGGTCACTGGGTCAGAACCCCACTGAGGCT
GAGCTCCAGGACATGATCAATGAAGTGGATGCTGATGGTAATGGAACTAT
TGATTTTCCTGAATTCCTAAACCTCATGGCCAGGAAGATGAAGGATACCG
ACTCTGAGGAGGAGCTGAAAGAGGCCTTCCGGGTTTTCGACAAGGATCAG
AATGGTTTCATCTCAGCTGCTGAACTGCGTCATGTGATGACAAACCTTGG
GGAGAAGCTTACTGATGAGGAAGTCGATGAGATGATAAGGGAAGCTGATG
TCGATGGTGATGGCCAGATAAATTATGAGGAATTTGTCAAGGTTATGATG
GCTAAGTAAGTGACTCCACCCAACCAAAACTTCCTGGAAAAAGAAGAAAT
TGGTTGATTAATATAATTGGTTCCCTAGTTTCCTTTTTTATAAATTTTAA
TTTGATAACTCTGAGGTCAAGCTAAAGGAAGCATGTTGTCTCCTGCTTCA
ATTTGTCTTGTGACTGGAGCAACTTTTCAGTTTTTT
back to top