CN182036

Overview
NameCN182036
Unique NameCN182036
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length534
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_PSEA8 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pseudomonas aeruginosa (strain LESB58) GN=metE PE=3 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 4.659e-46
Identity = 96/179 (53.63%), Postives = 121/179 (67.60%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKDE---AYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKP 530
            WLAFA QK  EV  LA+A +  +     A    + A QASR SSPR+   AVQ   AA+K SD +R +  + R+  Q+  L+LP  PTTTIGSFPQT  +R  R+ FK  K+SE +Y++ +  EI + V++QE+L +DVLVHGE ERNDMVEYF EQL G+ FT  GWVQSYGSRCVKP
Sbjct:  350 WLAFAVQKCREVAVLARAATEPEAAEVLAALEESRAVQASRASSPRIHKPAVQARLAAIKASDAQRRSPFAERIARQRAGLDLPAFPTTTIGSFPQTSSIRLARQSFKQGKLSEAEYIEAMHSEIRHAVQIQEQLGLDVLVHGEAERNDMVEYFAEQLDGYVFTRFGWVQSYGSRCVKP 528
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_XYLFT (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=metE PE=3 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.085e-46
Identity = 97/178 (54.49%), Postives = 116/178 (65.17%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKD-EAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL  + + E   +    A  +R+ SP+V    V +  AAL     RR T    R  AQQ  LNLP  PTTTIGSFPQT+++R  R +FK+ K+SE DY   +K E    V+ QEE+ +DVLVHGE ERNDMVEYFGEQL GF FT  GWVQSYGSRCVKPP
Sbjct:  348 WLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQRLAALTPDTTRRNTAYPQRSQAQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEIGLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPP 525
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_XYLFM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M12) GN=metE PE=3 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.085e-46
Identity = 97/178 (54.49%), Postives = 116/178 (65.17%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKD-EAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL  + + E   +    A  +R+ SP+V    V +  AAL     RR T    R  AQQ  LNLP  PTTTIGSFPQT+++R  R +FK+ K+SE DY   +K E    V+ QEE+ +DVLVHGE ERNDMVEYFGEQL GF FT  GWVQSYGSRCVKPP
Sbjct:  348 WLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQRLAALTPDTTRRNTAYPQRSQAQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEIGLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPP 525
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_XYLF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M23) GN=metE PE=3 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.085e-46
Identity = 97/178 (54.49%), Postives = 116/178 (65.17%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKD-EAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL  + + E   +    A  +R+ SP+V    V +  AAL     RR T    R  AQQ  LNLP  PTTTIGSFPQT+++R  R +FK+ K+SE DY   +K E    V+ QEE+ +DVLVHGE ERNDMVEYFGEQL GF FT  GWVQSYGSRCVKPP
Sbjct:  348 WLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQRLAALTPDTTRRNTAYPQRSQAQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEIGLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPP 525
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_NITOC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=metE PE=3 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.085e-46
Identity = 94/179 (52.51%), Postives = 119/179 (66.48%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKDEAY--FSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ EV  L +AL   +D      + +AAAQASR+ S R+    ++     +     RR      R   Q+++L LP  PTTTIGSFPQT D+R+ RR+FKA +I+E +Y + ++ EI   ++ QE   +DVLVHGE ERNDMVEYFGEQL GFAFT NGWVQSYGSRCVKPP
Sbjct:  347 WLAFAVQKIEEVATLKRALVEGRDVVAQALADSAAAQASRRQSERIYRPEIRARMTEVNEDMTRRRHKYPVRAQVQREELKLPLYPTTTIGSFPQTPDIRKARRDFKAGRIAEPEYRRQMEAEIARAIQAQESYGLDVLVHGEAERNDMVEYFGEQLEGFAFTQNGWVQSYGSRCVKPP 525
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.085e-46
Identity = 97/179 (54.19%), Postives = 120/179 (67.04%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKDEAY--FSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL+  +D      ++NAAA  SR+ SPRV N AV+ A A +      RA+  + R   Q  +LNLP  PTTTIGSFPQT ++R+ R  FKA  + E  Y   ++ EI   V+ QE L++DVLVHGE ERNDMVEYFGEQL G+AF+  GWVQSYGSRCVKPP
Sbjct:  351 WLAFALQKLDELKVLATALNEGRDTVADALAANAAAIESRRRSPRVNNPAVKAAIARIDARLGNRASPYAQRASKQSARLNLPAFPTTTIGSFPQTAEIRQARSRFKAGTLDEAGYRTAMQAEIERSVREQESLELDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPP 529
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_XYLFA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa GN=metE PE=3 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.038e-45
Identity = 95/178 (53.37%), Postives = 117/178 (65.73%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKD-EAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL  + + E   +    A  +R+ SP+V    V +  AAL     +R T    R  AQQ+ LNLP  PTTTIGSFPQT+++R  R +FK+ K+SE DY   +K E    ++ QEE+ +DVLVHGE ERNDMVEYFGEQL GF FT  GWVQSYGSRCVKPP
Sbjct:  348 WLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPEVAQRLAALTPDTTQRNTAYPQRSQAQQQTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYETFLKAETERCIRTQEEIGLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPP 525
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_BURVG (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=metE PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.770e-45
Identity = 98/179 (54.75%), Postives = 117/179 (65.36%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKDEAY--FSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL+  +D      + NAAA  SR+ SPRV N AV+ A A +      RA+    R  AQ  +L LP  PTTTIGSFPQT ++R  RR FKA ++ E  Y   +  EI   V+ QE L +DVLVHGE ERNDMVEYFGEQL G+AF+  GWVQSYGSRCVKPP
Sbjct:  351 WLAFALQKLAELKLLATALNQGRDTVADALAENAAAIESRRRSPRVHNPAVKSAVARIDARLGNRASAYPQRAAAQSARLKLPLFPTTTIGSFPQTAEIRDARRRFKAGELDEAGYRHAMHAEIERSVREQESLQLDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPP 529
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.770e-45
Identity = 95/179 (53.07%), Postives = 120/179 (67.04%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKDEAY--FSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL+  +D+     ++NAAA  SR+ SPRV N  V+ A A +      RA+  + R   Q  +LNLP  PTTTIGSFPQT ++R+ R  F+A  + E  Y   ++ EI   V+ QE L++DVLVHGE ERNDMVEYFGEQL G+AF+  GWVQSYGSRCVKPP
Sbjct:  351 WLAFALQKLDELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVNNPVVKAAIARIDAQLGNRASPYTQRASKQSARLNLPAFPTTTIGSFPQTAEIRQARSRFRAGALDEAGYRTAMRAEIERSVREQESLELDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPP 529
[Close]
BLAST of CN182036 vs. ExPASy Swiss-Prot
Match: METE_BURCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain AU 1054) GN=metE PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.770e-45
Identity = 95/179 (53.07%), Postives = 120/179 (67.04%), Query Frame = 3 
Query:    3 WLAFAAQKVVEVNALAKALSGQKDEAY--FSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPNLPTTTIGSFPQTMDLRRVRREFKAKKISEDDYVKTIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPP 533
            WLAFA QK+ E+  LA AL+  +D+     ++NAAA  SR+ SPRV N  V+ A A +      RA+  + R   Q  +LNLP  PTTTIGSFPQT ++R+ R  F+A  + E  Y   ++ EI   V+ QE L++DVLVHGE ERNDMVEYFGEQL G+AF+  GWVQSYGSRCVKPP
Sbjct:  351 WLAFALQKLDELKVLATALNEGRDKVADALAANAAAIDSRRRSPRVNNPVVKAAIARIDAQLGNRASPYTQRASKQSARLNLPAFPTTTIGSFPQTAEIRQARSRFRAGALDEAGYRTAMRAEIERSVREQESLELDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPP 529
The following BLAST results are available for this feature:
BLAST of CN182036 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 271
Match NameE-valueIdentityDescription
METE_PSEA84.659e-4653.635-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFT6.085e-4654.495-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFM6.085e-4654.495-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLF26.085e-4654.495-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NITOC6.085e-4652.515-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ6.085e-4654.195-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_XYLFA1.038e-4553.375-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURVG1.770e-4554.755-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH1.770e-4553.075-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCA1.770e-4553.075-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001N16_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001N16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN182036 ID=CN182036; Name=CN182036; organism=Citrus sinensis; type=EST; length=534bp
GCTGGCTTGCATTTGCTGCACAGAAAGTAGTTGAAGTAAATGCATTGGCC
AAGGCATTGTCTGGGCAAAAGGATGAGGCATACTTCTCTTCTAATGCTGC
AGCTCAGGCTTCAAGAAAATCCTCCCCAAGGGTGACAAATGAGGCTGTCC
AAAAGGCTGCTGCTGCTTTGAAGGGTTCTGACCATCGCCGAGCCACAAAT
GTCAGTGCTAGGTTGGATGCTCAGCAGAAGAAGTTGAACTTGCCAAATCT
TCCTACCACCACCATTGGATCTTTCCCACAAACGATGGATCTCAGAAGAG
TCCGTCGTGAATTCAAGGCCAAGAAAATCTCTGAGGATGACTATGTCAAG
ACTATCAAGGAGGAAATCAACAACGTTGTCAAGCTCCAGGAAGAGCTTGA
CATTGATGTTTTGGTGCATGGAGAACCAGAGAGGAATGATATGGTTGAGT
ACTTCGGTGAGCAATTATCTGGTTTTGCCTTCACTGTGAATGGGTGGGTT
CAATCTTATGGCTCCCGCTGTGTCAAGCCTCCTA
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