CN188821

Overview
NameCN188821
Unique NameCN188821
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length757
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: INO80_ASPTN (Putative DNA helicase ino80 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=ino80 PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.906e-15
Identity = 40/104 (38.46%), Postives = 61/104 (58.65%), Query Frame = -1
Query:  440 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDD 751
            M+D + +    +     R+DG T    R+  V +FQ++ ++   +LS +AGG+G+ LTAA TVIF +  W P    QA DRAHR+GQ   V VY L+   T+++
Sbjct: 1427 MIDLMEEYLTYRNYKYCRLDGSTKLEDRRDTVADFQQRPEIFVFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLITRGTIEE 1530          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: INO80_ASPNC (Putative DNA helicase ino80 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN=ino80 PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.906e-15
Identity = 40/104 (38.46%), Postives = 61/104 (58.65%), Query Frame = -1
Query:  440 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDD 751
            M+D + +    +     R+DG T    R+  V +FQ++ ++   +LS +AGG+G+ LTAA TVIF +  W P    QA DRAHR+GQ   V VY L+   T+++
Sbjct: 1434 MIDLMEEYLTYRNYKYCRLDGSTKLEDRRDTVADFQQRPEIFVFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLITRGTIEE 1537          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: STH1_YEAST (Nuclear protein STH1/NPS1 OS=Saccharomyces cerevisiae GN=STH1 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 6.473e-15
Identity = 42/122 (34.43%), Postives = 68/122 (55.74%), Query Frame = -1
Query:  389 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKD-DVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDDIVWDVVRSKLENLGQVL 751
            ++D +      K +  +R+DG T    R  ++  F   D D    +LS +AGG+GL L  A TVI  +  W P   +QA+DRAHRIGQ + V +  L+  D+V++++ +    KL+  G+V+
Sbjct:  819 VMDIMEDFLRMKDLKYMRLDGSTKTEERTEMLNAFNAPDSDYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITTDSVEEVILERAMQKLDIDGKVI 940          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: INO80_GIBZE (Putative DNA helicase INO80 OS=Gibberella zeae GN=INO80 PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 6.473e-15
Identity = 45/131 (34.35%), Postives = 69/131 (52.67%), Query Frame = -1
Query:  359 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDDIVWDVVRSKLENLGQVLDGHENSLEVS 751
            M+D + +    +     R+DG T    R+  V +FQ + ++   +LS +AGG+G+ LT A TVIF +  W P    QA DRAHR+GQ   V VY L+   T+++ +      K E    V+ G   S++ S
Sbjct: 1654 MIDMMEEYLTYRNYKYCRLDGSTKLEDRRDTVHDFQTRPEIFIFLLSTRAGGLGINLTTADTVIFYDSDWNPTIDSQAMDRAHRLGQTKQVTVYRLITRGTIEERIRKRAMQKEEVQRVVIQGGGASVDFS 1784          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: INO80_CANAL (Putative DNA helicase INO80 OS=Candida albicans GN=INO80 PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 6.473e-15
Identity = 43/115 (37.39%), Postives = 64/115 (55.65%), Query Frame = -1
Query:  407 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDDIVWDVVRSKLE 751
            M+    +    K    IR+DG T   SR+ +V  +Q   ++   +LS +AGG+GL LT+A TVIF +  W P    QA DRAHRIGQ   V V+ L+  +T++  + +  + K E
Sbjct: 1264 MMQIFEEYLAYKSYKYIRLDGSTTIESRREMVQAWQTNPEIFIFMLSTRAGGLGLNLTSADTVIFYDSDWNPTIDSQAMDRAHRIGQTKQVKVFRLVTRNTIEQKILERAKEKEE 1378          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: INO80_NEUCR (Putative DNA helicase ino-80 OS=Neurospora crassa GN=ino-80 PE=3 SV=3)

HSP 1 Score: 80.4925 bits (197), Expect = 1.104e-14
Identity = 43/123 (34.96%), Postives = 66/123 (53.66%), Query Frame = -1
Query:  383 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDDIVWDVVRSKLENLGQVLDG 751
            M+D + +    +     R+DG T    R+  V +FQ + ++   +LS +AGG+G+ LT+A TVIF +  W P    QA DRAHR+GQ   V VY L+   T+++ +      K E    V+ G
Sbjct: 1726 MIDLMEEYLTYRNYKYCRLDGSTKLEDRRDTVADFQTRPEIFIFLLSTRAGGLGINLTSADTVIFYDSDWNPTIDSQAMDRAHRLGQTKQVTVYRLITRGTIEERIRKRAMQKEEVQRVVITG 1848          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: SSL1_CAEEL (Helicase ssl-1 OS=Caenorhabditis elegans GN=ssl-1 PE=2 SV=4)

HSP 1 Score: 79.7221 bits (195), Expect = 1.883e-14
Identity = 40/104 (38.46%), Postives = 58/104 (55.77%), Query Frame = -1
Query:  440 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDD 751
            MLD +            R+DG T    RQA++  F     V   +LS ++GGVG+ LT A TVIF +  W P    QA+DR HRIGQ  +V++Y L++  T+++
Sbjct: 1216 MLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEE 1319          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: SRCAP_HUMAN (Helicase SRCAP OS=Homo sapiens GN=SRCAP PE=1 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 1.883e-14
Identity = 40/104 (38.46%), Postives = 59/104 (56.73%), Query Frame = -1
Query:  440 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDD 751
            MLD + +         +R+DG T    RQAL+  F     +   +LS ++GGVG+ LT A TV+F +  W P    QA+DR HRIGQ   V++Y L++  TV++
Sbjct: 2071 MLDVLEQFLTYHGHLYLRLDGSTRVEQRQALMERFNADKRIFCFILSTRSGGVGVNLTGADTVVFYDSDWNPTMDAQAQDRCHRIGQTRDVHIYRLISERTVEE 2174          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: RAD5_USTMA (DNA repair protein RAD5 OS=Ustilago maydis GN=RAD5 PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.883e-14
Identity = 48/139 (34.53%), Postives = 71/139 (51.08%), Query Frame = -1
Query:  347 LDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVL---SMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDDIVWDVVRSKLENLGQVL--DGHENSLEVSSSQI 748
            LD I  +  + + H +R+DG TP   R  LV EFQ        VL   S+KAGGVGL LTAA+ +   +  W      QA DR HR GQ S V+V+  +  D+++D +  + + K   +   L  D H +  + +S  +
Sbjct: 1240 LDLIEPVLTRYRFHFLRLDGSTPQKVRDKLVLEFQSPSPTNHVVLFLISLKAGGVGLNLTAANKIWLLDFWWNSSIENQAIDRIHRFGQTSPVSVFRYIIKDSIEDRILLIQKRKDMLIKHALNTDNHPHGTKPNSEML 1378          
BLAST of CN188821 vs. ExPASy Swiss-Prot
Match: CHD1L_HUMAN (Chromodomain-helicase-DNA-binding protein 1-like OS=Homo sapiens GN=CHD1L PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.883e-14
Identity = 46/123 (37.40%), Postives = 68/123 (55.28%), Query Frame = -1
Query:  383 MLDAIHKLFLKKKVHCIRIDGGTPPASRQALVTEFQEKDDVKAAVLSMKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRAHRIGQVSSVNVYYLLANDTVDDIVWDVVRSKLENLGQVLDG 751
            MLD +      +     R+DG      R   +  F ++  +   +LS +AGGVG+ LTAA TVIF +  + P + +QA  RAHRIGQ  SV V  L+  DTV++IV+    SKL+    +++G
Sbjct:  375 MLDILQDYMDYRGYSYERVDGSVRGEERHLAIKNFGQQP-IFVFLLSTRAGGVGMNLTAADTVIFVDSDFNPQNDLQAAARAHRIGQNKSVKVIRLIGRDTVEEIVYRKAASKLQLTNMIIEG 496          
The following BLAST results are available for this feature:
BLAST of CN188821 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 138
Match NameE-valueIdentityDescription
INO80_ASPTN2.906e-1538.46Putative DNA helicase ino80 OS=Aspergillus terreus... [more]
INO80_ASPNC2.906e-1538.46Putative DNA helicase ino80 OS=Aspergillus niger (... [more]
STH1_YEAST6.473e-1534.43Nuclear protein STH1/NPS1 OS=Saccharomyces cerevis... [more]
INO80_GIBZE6.473e-1534.35Putative DNA helicase INO80 OS=Gibberella zeae GN=... [more]
INO80_CANAL6.473e-1537.39Putative DNA helicase INO80 OS=Candida albicans GN... [more]
INO80_NEUCR1.104e-1434.96Putative DNA helicase ino-80 OS=Neurospora crassa ... [more]
SSL1_CAEEL1.883e-1438.46Helicase ssl-1 OS=Caenorhabditis elegans GN=ssl-1 ... [more]
SRCAP_HUMAN1.883e-1438.46Helicase SRCAP OS=Homo sapiens GN=SRCAP PE=1 SV=2[more]
RAD5_USTMA1.883e-1434.53DNA repair protein RAD5 OS=Ustilago maydis GN=RAD5... [more]
CHD1L_HUMAN1.883e-1437.40Chromodomain-helicase-DNA-binding protein 1-like O... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006N06_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006N06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188821 ID=CN188821; Name=CN188821; organism=Citrus sinensis; type=EST; length=757bp
CTTTTTTTATAAACGATGTTATTGTATTCATCTACAAAACAGAGGAATAT
CCCTATAAAGCCTCATGTCAGTATTACACTTACACTGCCTGTCATATAGA
GATTCTTGTCTCTCACTAATTTGGAGTAGTAATCAATGTTCTCTGAGGCT
CCGCATATCAAAAGGGCACATTTTCATGTGTCTGTGTACATGTGATGCAT
AGACCGACGCATCTCAAAATATACATTTTATAGTGTGTGGATTAGAGCTT
TCGGTATTTTAGCTTCTGCTGATGTTCAGAGTCATCCACATTGTTGCAAC
GCTTCAGGAAGGAGTCGAGTGTTTTCTGCTTTGCAGGGCTGCTTCTTATT
TGACTGCTTGAAACTTCCAAACTATTTTCATGACCATCAAGCACCTGTCC
CAAATTTTCCAACTTGCTCCGAACAACATCCCATACTATGTCATCAACTG
TGTCATTTGCCAGTAGGTAGTATACATTTACGGAAGATACCTGGCCGATC
CTGTGAGCACGATCTTCAGCTTGAATGAGATCACCTGGAGTCCATGACAA
CTCCGCGAAAATAACAGTGCTTGCAGCCGTTAATGTTAAACCAACACCTC
CGGCTTTCATAGATAGCACTGCTGCCTTGACATCATCTTTCTCCTGAAAT
TCAGTAACCAAAGCTTGTCTTGAAGCAGGAGGGGTACCCCCATCAATCCG
AATGCAATGCACTTTCTTCTTAAGAAAAAGTTTATGTATTGCATCAAGCA
TGGGCTG
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